Evaluation of rice cultivars for resistance to rice yellow mottle virus

Rice yellow mottle virus (RYMV), which is only found in Africa, threatens rice farming on the continent. A local Oryza sativa cultivar collected from Burkina Faso (named BM24), was evaluated with that of well known highly resistant and tolerant cultivars. Firstly, three RYMV isolates were used to characterise the differential interaction within the cultivars. Secondly, disease kinetics of symptom expression and virus titer on leaves at 21 days after inoculation were assessed using the BF1 isolate. Thirdly, the allelic profile of O. sativa varieties using SSR marker RM101 located on chromosome 12 was also assessed. IR64 showed susceptibility to all isolates; while Tog5681 was resistant to all isolates. Ng122 overcame the resistance of Gigante, with mild leaf symptoms at 42 dpi. Azucena and BM24 had, therefore, different resistance level regarding the three isolates (Ng117b, Ng122 and Ng144). When infected with the isolate, BF1, BM24 and Azucena exhibited same resistance patterns in early growth stages with delayed of symptoms appearance, but BM24 outperformed Azucena at later stages. The virus content in the two accessions, at 14 days post inoculation, was statistically different with BM24, showing less virus compared to Azucena. However, the two accessions depicted an identical allelic profile at RM101 locus

Otomastoïdites aiguës dans le Service d’ORL du CHU Yalgadode Ouagadougou: à propos de 44 cas

Analyser les aspects diagnostiques et thérapeutiques des otomastoïdites dans le Service d’ORL du CHU-YO de Ouagadougou. Méthode : Il s’est agit d’une étude rétrospective de 10 ans, qui a porté sur 44 cas. Résultats: l’incidence annuelle était de 4,4 cas et l’âge moyen de 13,41 ans. Les mastoïdites étaient majoritairement fistulisées (59,09%) et les principaux germes incriminés étaient Staphylococcus aureus (35,29%), Proteus mirabilis (23,53%) et Pseudomonas aeruginosa (23,53%). Ils étaient surtout sensibles à la ciprofloxacine (82,35%) et la ceftriaxone (58,82%). Le traitement a été médico-chirurgical dans 86,36% des cas et l’évolution favorable dans 72,73%. Douze cas (27,27%) de décès par complication systémique et endocrânienne ont été enregistrés. Conclusion : Il convient de prioriser les mesures préventives et la prise en charge précoce des otites infectieuses.Mots clés: Otomastoïdite, épidémiologie, diagnostic, thérapeutique

Hernie diaphragmatique post-traumatique de l’enfant: à propos d’un cas au Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle de Ouagadougou

La hernie diaphragmatique post-traumatique est une urgence chirurgicale rare chez l’enfant mais pouvant mettre rapidement en jeu le pronosticvital. Les auteurs rapportent le cas d’un garçon de 04 ans admis aux urgences pour douleur abdominale suite à une contusion thoraco-abdominale par accident de la voie publique. Le bilan radiologique initial a consisté en une échographie abdominale qui a révélé un hémopéritoine de petite abondance sans lésion focale. Douze heures après son admission, le patient a présenté une détresse respiratoire avec tableau clinique depneumothorax gauche qui a nécessité une exsufflation en urgence. Le diagnostic de hernie diaphragmatique gauche a été posé à la radiographiedu thorax réalisée après la ponction. L’enfant a bénéficié d’une cure  chirurgicale. L’évolution a été favorable. La hernie diaphragmatique posttraumatique, bien que rare chez l’enfant, devrait être systématiquement recherchée par une radiographie thoracique ou un scanner  thoracoabdominal devant tout traumatisme abdominal avec hyper pression. Son traitement est chirurgical

Proximal ischemia is a frequent cause of exercise-induced pain in patients with a normal ankle to brachial index at rest

BACKGROUND: Excluding a vascular origin of exercise-related pain is often difficult in clinical practice. Recent papers have underlined the frequent association of concurrent lumbar spine degenerative disease and peripheral arterial disease. Furthermore, even when suspected, isolated exercise-induced proximal ischemia is difficult to diagnose. Measurement of transcutaneous oxygen pressure (tcpO2) is an interesting and accurate method to differentiate proximal (buttock) from distal (calf) regional blood flow impairment (RBFI) during exercise. OBJECTIVES: We searched for isolated proximal-without-distal RBFI as a possible cause of claudication, in patients with borderline (ABI-b: 0.91 - 0.99) or normal (ABI-n: 1.00 to 1.40) ankle to brachial index at rest. STUDY DESIGN: Retrospective cohort design study. We analyzed patients referred to our laboratory with symptom limiting claudication and an ankle brachial index within normal limits. SETTING: University-based exercise-investigation center. METHODS: Over a 12-year period, we identified 463 patients referred to our laboratory that had their lowest resting ABI between 0.90 and 1.40. The tcpO2 on chest, buttocks, and calves were recorded during treadmill walking tests (3.2 km/h, 10% slope) in 220 ABI-b and 243 ABI-n unique consecutive patients complaining of limiting claudication (each patient\u27s ABI was the lowest of the 2 legs). Limiting claudication was defined as the reported inability to walk 1 kilometer without stopping. A DROP index (limb tcpO2-changes minus chest tcpO2-changes from rest) below -15 mmHg was used to indicate a positive result (i.e. exercise-induced RBFI). RESULTS: Treadmill exercise showed evidence for proximal or distal RBFI, of at least one side, in 128 out of 220 patients (58.2%) and in 86 out of 243 (35.4%) patients with ABI-b and ABI-n, respectively. Isolated proximal-without-distal RBFI was found in 32 out of the 128 (25.0 %) positive tests in ABI-b and 32 out of the 86 (37.2%) positive tests in ABI-n patients. LIMITATIONS: Study limitations include the absence of systematic follow-up of diagnosed patients and absence of systematic search for cardio-respiratory co-morbid conditions. CONCLUSION: Isolated proximal-without distal RBFI is found in approximately one out of 7 patients complaining of symptom limiting claudication with a borderline or normal resting ABI. Exercise-tcpO2 may help to discriminate patients with arterial claudication that could benefit from invasive vascular investigations and procedures

Calf muscle stimulation with the Veinoplus device results in a significant increase in lower limb inflow without generating limb ischemia or pain in patients with peripheral artery disease

OBJECTIVE: Increase in arterial inflow to the lower limbs is important to obtain functional improvement in peripheral artery disease (PAD) patients with claudication. The aim of this study was to assess the effect of electrical stimulation of calf muscles on arterial inflow and tissue oxygen content in PAD in the area of stimulation.METHODS: Fifteen adult patients [mean (standard deviation) age, 62 (12 ) years; height, 165 (8)cm; weight, 76 (13) kg; lowest ankle-brachial index 0.66 (0.19)] with stable arterial claudication were recruited. All patients performed a treadmill test (3.2 km/h, 10% slope) associated with a transcutaneous oximetry test expressed as decrease from rest of oxygen pressure (DROP) index values (calf changes minus chest changes from rest) with a maximum walking distance (median [25th/75th percentiles]) of 295 [133-881] m. The DROP index on the symptomatic side was -25 [-18/-34] mm Hg. On another day the patients underwent electrical stimulation in the seated position on the leg that was the most symptomatic on the treadmill. After resting values were recorded, the gastrocnemius was stimulated for 20minutes at increasing contraction rates at 5-minute steps of 60, 75, 86, and 100bpm on the most symptomatic side. Arterial blood inflow with duplex Doppler ultrasound scanning of the femoral artery, DROP transcutaneous oxygen pressure value, and oxygen concentration (O2Hb) from the near-infrared spectroscopic signal of the calf were recorded on both sides. Patients were instructed to report eventual contraction-induced pain in the stimulated calf. Results are given as mean (standard deviation) or median [25th/75th percentiles] according to distribution, and the level of statistical significance was set at P < .05 on two-tailed tests. RESULTS: Lower limb inflow (mL/min) was 64 [48/86] vs 63 [57/81] (P> .05) before stimulation, 123 [75/156] vs 57 [44/92] (P < .01) at 60bpm, 127 [91/207] vs 49 [43/68] (P < .01) at 75bpm, 140 [84/200] vs 57 [45/71] (P < .01) at 86bpm, and 154 [86/185] vs 55 [46/94] (P < .01) at 100bpm on the stimulated vs nonstimulated limb, respectively. No apparent decrease or significant leg difference was observed in DROP index or O2Hb values. None of the patients reported contraction-induced pain in the leg. CONCLUSIONS: Electrical stimulation of calf muscle with the Veinoplus device results in a significant increase of arterial inflow without measurable muscle ischemia or pain. Potential use of this device as an adjuvant treatment to improve walking capacity in PAD patients remains to be evaluated

Heart rate recovery after constant-load exercise tests is decreased in proportion to the importance (severity and diffusion) of exercise-induced lower-limb ischaemia

BACKGROUND: Conditions that may influence heart rate recovery at 1 min of recovery from exercise (HRR1: end-exercise heart rate minus heart rate 1 min after exercise) are not fully understood. We hypothesized that the \u27importance\u27 (both local severity and regional diffusion) of peripheral skeletal muscle ischaemia is associated with low HRR1. DESIGN AND METHODS: In 529 patients with suspected or confirmed peripheral vascular disease not receiving beta-blockers (61.4 +/- 11.3 years old), we retrospectively studied the relationship of HRR1 to exercise-induced changes in transcutaneous oxygen DROP index (limb changes minus chest changes from rest). The sum of DROP indices observed on both calves and both buttocks (DROPtot) provides the unique opportunity to estimate both the severity and the diffusion of exercise-induced ischaemia on the right and left side simultaneously. It was used during a constant-load treadmill test (3.2 km h(-1) ; 10% grade) to classify patients in quartiles, the fourth quartile representing the more \u27important\u27 ischaemias.RESULTS: There was an inverse relationship between quartiles of DROPtot and HRR1, even after adjustment for heart rate reserve (Delta HR: end-exercise minus resting heart rate), age (60 years), gender, body mass index, treadmill maximal walking distance and ankle brachial index: adjusted R = 0.629; P<0.0001. CONCLUSIONS: During constant-load treadmill testing, DROPtot, an index of the \u27importance\u27 of exercise-induced lower-limb ischaemia, correlates with HRR1. Whether HRR1 is improved in proportion of DROPtot improvement in patients undergoing surgery or rehabilitation for peripheral artery disease is a fascinating issue for future studies

Using Seasonal Forecast as an Adaptation Strategy: Gender Differential Impact on Yield and Income in Senegal

The use of seasonal forecast has been demonstrated as a good option to reduce the effects of climate variability in sub-Saharan African countries. However, its use, benefits and interests may be different depending on gender. This paper aims at analyzing the gender differential impact of the use of seasonal forecast on the main crop yields (rice, maize, sorghum, millet and groundnut) and farm income in Senegal. We collected data from 1481 farmers (44% women) in four regions of Senegal. We applied the counterfactual outcomes framework of modern evaluation theory to estimate the local average treatment effect (LATE) of the use of the seasonal forecast on crop yield and farm income. The results showed a significant impact of the use of the seasonal forecast (SF) in the main crop yields and the agricultural income for farmers in Senegal. This impact varies according to the sex and the type of the crops. The users (men and women) of the seasonal forecast gained on average 158 kg/ha and 140 kg/ha more yield than the non-users, respectively, for millet and rice crops. The impact of the use of SF is greater for men on millet (202.7 kg/ha vs. 16.7 kg/ha) and rice (321.33 kg/ha vs

Vasodilator effect of Zanthoxylum zanthoxyloïdes, Calotropis procera and FACA, a mixture of these two plants

Root bark extracts from Zanthoxylum zanthoxyloides Lam (Rutaceae) and Calotropis procera Aït (Asclepiadaceae) were used in Burkina Faso folk medicine for several diseases particularly sickle cell anemia. Authors reported here vasorelaxant effect of these plants on rat isolated aorta. Z. zanthoxyloïdes (cumulative addition) produces a concentration-dependent relaxation on the aorta. Maximal effects are respectively of 60.34 ± 2.34 and 100% in the presence and in the absence of endothelium. C. procera extract induces a more relaxing effect on endothelium-denuded aorta (Emax = 59.78 ± 2.13% and 100% in presence and absence of endothelium respectively). FACA, the mixture of these two plants also induces vasorelaxation (Emax = 100%), with better effect in the presence of endothelium (EC50 = 2.76 ± 0.38 mg/mL and 4.90 ± 0.69 mg/mL in presence and absence of endothelium respectively). Endothelium-dependent vasodilator effect of FACA was inhibited by L-NAME; this clearly indicates that NO is involved in aorta relaxation process by FACA. Taken together this study revealed that FACA and its components possess vasodilator effect. This vascular property of FACA may be involved in the amendment of sickle cell crisis through inhibition of vaso-occlusion process.Keywords: Sickle cell anemia, phytotherapy, rat aorta, endotheliu

Evaluation de la Translocation du Cadmium, du Cuivre, du Plomb et du Zinc par Zea mays L. cultivé Sur un sSol Ferrugineux Tropical dans l’Ouest du Burkina Faso

L’utilisation des déchets urbains comme fertilisants organiques peut être à l’origine d’une contamination du sol en éléments traces métalliques. Le maïs, l’une des céréales les plus consommées au Burkina Faso, pourrait accumuler ces éléments toxiques et provoquer des problèmes sanitaires à l’homme à travers leur consommation. Pour évaluer le potentiel de translocation du maïs, un essai est mis en place dans le périmètre maraicher de Sakabi, dans la commune de Bobo Dioulasso. Pour ce faire, des parcelles ont été confectionnées et organisées selon un dispositif en bloc complètement randomisé avec six répétitions. Des doses croissantes de  20, 40 et 60 tonnes de déchet par hectare ont été apportées dans les différentes parcelles pour constituer les traitements. Les échantillons de sol et la biomasse végétale ont été prélevés pour déterminer les teneurs en éléments traces métalliques. Les résultats des analyses montrent que les sols sont contaminés en métaux lourds et ont contribué à la translocation de ces derniers dans les différents organes du maïs. Les facteurs de translocation sont supérieurs à 1 dans tous les organes exception faite pour les tiges donc les valeurs sont comprises entre 0,5-0,8 et 0,76-0,93 respectivement pour le cadmium et le cuivre. En outre la corrélation de Pearson a montré une forte relation entre les teneurs en métaux lourds dans les différents organes. Lorsque le cadmium dans le sol augmente de 1 mg il y a une forte probabilité qu’il augmente de 0,22 mg dans les racines, 1,33 mg dans les tiges, 0, 31 mg dans les feuilles et 0,56 mg dans les grains. De même, au niveau du cuivre, il peut augmenter de 2,56 mg, 0,28 mg, 0,57 mg respectivement pour les racines, tiges et feuilles pour une augmentation de 1 mg de Cu dans le sol. Au niveau du plomb, il y a une forte probabilité que les teneurs dans les feuilles et dans les grains augmentent respectivement de 6,84 mg et 5,81 mg pour une augmentation de 1 mg de plomb dans le sol. Pour le cas du zinc, il peut augmenter de 0,61 mg, 0,17 mg, 0,15 mg respectivement pour les tiges, feuilles et grains pour une augmentation de 1 mg dans le sol.   The use of municipal waste as organic fertilizers may result in contamination of the soil with trace metals. Corn, one of the most widely consumed cereals in Burkina Faso, could accumulate these elements and cause health problems for humans. To assess the translocation potential of maize, a trial is being carried out in the Sakabi vegetable perimeter. To do this, plots were made and organized according to a completely randomized block device with six repetitions. Increasing amounts of waste  20, 40 and 60 tons per hectare were brought into the different plots to constitute the treatments. Soil samples and plant biomass were collected to determine trace metal content. The results of the analyzes show that the soils are contaminated with heavy metals and have contributed to their translocation to the different organs of the maize. The translocation factors are greater than 1 in all organs except for the rods, so the values are between 0.5-0.8 and 0.76-0.93 respectively for cadmium and copper. In addition, the Pearson correlation showed a strong relationship between the heavy metal contents in the different organs. When cadmium in soil increases by 1 mg, there is a high probability that it will increase by 0.22 mg in roots, 1.33 mg in stems, 0.31 mg in leaves, and 0.56 mg in grains. Similarly, at the level of copper, it can increase by 2.56 mg, 0.28 mg, 0.57 mg respectively for roots, stems and leaves for an increase of 1 mg of Cu in the soil. At this level, there is a high probability that leaf and grain levels will increase by 6.84 mg and 5.81 mg, respectively, for an increase of 1 mg lead in soil. For zinc, it can increase by 0.61 mg, 0.17 mg, 0.15 mg for stems, leaves and grains respectively for an increase of 1 mg in the soil