RNase T1 mimicking artificial ribonuclease

Recently, artificial ribonucleases (aRNases)—conjugates of oligodeoxyribonucleotides and peptide (LR)4-G-amide—were designed and assessed in terms of the activity and specificity of RNA cleavage. The conjugates were shown to cleave RNA at Pyr-A and G–X sequences. Variations of oligonucleotide length and sequence, peptide and linker structure led to the development of conjugates exhibiting G–X cleavage specificity only. The most efficient catalyst is built of nonadeoxyribonucleotide of unique sequence and peptide (LR)4-G-NH2 connected by the linker of three abasic deoxyribonucleotides (conjugate pep-9). Investigation of the cleavage specificity of conjugate pep-9 showed that the compound is the first single-stranded guanine-specific aRNase, which mimics RNase T1. Rate enhancement of RNA cleavage at G–X linkages catalysed by pep-9 is 108 compared to non-catalysed reaction, pep-9 cleaves these linkages only 105-fold less efficiently than RNase T1 (kcat_RNase T1/kcat_pep-9 = 105)

The minimal compound-complex sentence hierarchy

This article deals with the problem of the study of the subsystem of such type of the Multiple Compound-Complex Sentence as a minimal compound-complex sentence. The authors analyze the formal structure of the multiple compoundcomplex sentences and try to build up the structural models hierarchy of Minimal Compound-Complex SentenceyesBelgorod State Universit

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A(2) inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A(2) by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.Peer reviewe

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed

Issues of invention in the construction sector in conditions of its digitalization

The article considers the possibility of creating a digital environment of engineering creativity for organizing training for students of construction universities based on cloud technologies, which is an urgent task in the context of the implementation of the State program "Digital Economy of the Russian Federation". The purpose of the study is to develop theoretical grounds for creating a cloud digital environment of engineering creativity (DEEC) for interaction between participants in the creative process in construction universities. The practical value of the study lies in the possibility of applying the proposed solution when implementing distance learning for students of civil engineering universities

The economic efficiency of european football clubs - Data Envelopment Analysis (DEA) approach

The relevance of this paper lies in the fact that football business has grown significantly in the past 20 years and football clubs have become large companies, which in an effort to be profitable and successful on the field need to improve the efficiency of their business. The aim of this article is to measure economic efficiency of 48 big European football clubs and assess the relationship between efficiency and different financial and sportive indicators (variables). To measure efficiency, we used both widely used Data Envelopment Analysis (DEA) method and its extensions: DEA Super-efficiency and DEA Cross-efficiency models. The results showed that these methods can successively be applied to football clubs’ efficiency measurement and the analysis of the them can help to explain why some clubs are efficient or inefficient and which factors affects the efficiency. This paper will be interesting football clubs’ managers, football analytics, economists and other people interested in football business because we combine in it the most interesting ideas and methods about football clubs’ efficiency measurement. © 2016 Pyatunin et al

Видове різноманіття та особливості поширення дереворуйнівних грибів у парку культури і відпочинку імені Михайла Чекмана міста Хмельницького

The results of studies of the species composition of wood-destroying fungus in the Park of Recreation and Leisure named after Mykhailo Chekman in the city of Khmelnytskyi are presented here. In the Park, 29 species of wood-destroying fungus were found, which are widespread on 14 tree species. The most common types of destroying fungus were: Fomitopsis pinicola, Bjercandera adusta, Bjercandera fumosa, Phellinus igniarius, and Stereum hirsutum. These types of wood-destroying fungus are found on almost all tree species of the park which have been damaged by the fungi. The tree species, which are most susceptible to wood-destroying fungus, are: Betula pendula Roth., Fraxinus excelsior L., Salix alba L., Populus nigra and Quercus robur L. The lowest occurance of wood-destroying fungi was found on вільсі чорній and грабові звичайному. It has been proved that the role of wood-destroying fungus in urban plantations is negative. Most species of wood-destroying fungus cause a variety of rot. Such trees have no external signs of weakening for a long time. It is possible to find affected trees with wood-destroying mushrooms by the presence of fruit bodies. The methods of dealing with wood-destroying fungus are presented here. The main method is conducting periodic forest-pathological surveys to detect the species composition of wood-destroying fungus and the degree of affliction of plantations. In the park, along with the implementation of sanitary measures, the individual protection of trees is important. Methods of protection are; wound healing, wound rubbing after pruning and filling of tree hollows. Protection of trees on the basis of chemical methods should be carried out with single defeat. Measures to regulate recreational loads and prevent mechanical and other tree damage are also important in Chekman Park. The obtained research results broaden the idea of the species diversity of wood-destroying fungi in the park plantations of Khmelnytskyi and can be used in the organization and implementation of forest-pathological monitoring, as well as determining the locations of future sanitary and recreational activities.Наведено результати дослідження видового складу дереворуйнівних грибів у Парку культури і відпочинку ім. Михайла Чекмана міста Хмельницького та біоекологічні особливості їх поширення. У Парку культури і відпочинку ім. Михайла Чекмана виявлено 29 видів дереворуйнівних грибів, які поширені на 14 видах деревних порід. Найпоширенішими виявилися таки види дереворуйнівних грибів: трутовик облямований, б'єркандера обвуглена, б'єркандера димчаста, трутовик несправжній та стереум жорстковолосистий. Ці види дереворуйнівних грибів трапляються майже на всіх деревних видах парку, які пошкоджені дереворуйнівними грибами. Ці гриби трапляються поодиноко на видах дерев I та ІІ стадій деструкції, дуже рідко – на III стадії. Встановлено деревні породи, які найбільше піддаються ураженню дереворуйнівними грибами, це такі види: береза повисла, ясен звичайний, верба біла, тополя чорна та дуб звичайний. Доведено, що роль дереворуйнівних грибів у міських насадженнях негативна. Більшість видів дереворуйнівних грибів спричиняють різноманітну гниль. Такі дерева довгий час не мають зовнішніх ознак ослаблення. Встановити вражені дерева дереворуйнівними грибами можна тільки за наявністю плодових тіл. Запропоновано основні методи боротьби з дереворуйнівними грибами у парку

Assessment of the Preschoolers and Younger Schoolchildren Readiness for Artistic Creativity by the Rank Standardization Method

Статья посвящена проблеме оценки готовности дошкольников и младших школьников к художественному творчеству, которое является основой художественно-эстетического развития детей. Благодаря художественному творчеству ребенок формирует свою картину окружающего мира, приобретает индивидуальный опыт в освоении различных культурных средств и формируется как эстетически развитая, творчески активная личность.The article is devoted to the problem of assessing the readiness of preschoolers and primary schoolchildren for artistic creativity, which is the basis of the artistic and aesthetic development of children. Thanks to artistic creativity, the child forms his own picture of the world around him, acquires individual experience in the development of various cultural means and is formed as an aesthetically developed, creatively active person