Collaborative Lubricating Oil Study on Emissions: November 28, 2006 - March 31, 2011

The Collaborative Lubricating Oil Study on Emissions (CLOSE) project was a pilot investigation of how fuels and crankcase lubricants contribute to the formation of particulate matter (PM) and semi-volatile organic compounds (SVOC) in vehicle exhaust. As limited vehicles were tested, results are not representative of the whole on-road fleet. Long-term effects were not investigated. Pairs of vehicles (one normal PM emitting, one high-PM emitting) from four categories were selected: light-duty (LD) gasoline cars, medium-duty (MD) diesel trucks, heavy-duty (HD) natural-gas-fueled buses, and HD diesel buses. HD vehicles procured did not exhibit higher PM emissions, and thus were labeled high mileage (HM). Fuels evaluated were non-ethanol gasoline (E0), 10 percent ethanol (E10), conventional low-sulfur TxLED diesel, 20% biodiesel (B20), and natural gas. Temperature effects (20 degrees F, 72 degrees F) were evaluated on LD and MD vehicles. Lubricating oil vintage effects (fresh and aged) were evaluated on all vehicles. LD and MD vehicles were operated on a dynamometer over the California Unified Driving Cycle, while HD vehicles followed the Heavy Duty Urban Dynamometer Driving Schedule. Regulated and unregulated emissions were measured. Chemical markers from the unregulated emissions measurements and a tracer were utilized to estimate the lubricant contribution to PM

Measurement of ambient aerosol hydration state at Great Smoky Mountains National Park in the southeastern United States

We present results from two field deployments of a unique tandem differential mobility analyzer (TDMA) configuration with two primary capabilities: identifying alternative stable or meta-stable ambient aerosol hydration states associated with hysteresis in aerosol hydration behavior and determining the actual Ambient hydration State (AS-TDMA). This data set is the first to fully classify the ambient hydration state of aerosols despite recognition that hydration state significantly impacts the roles of aerosols in climate, visibility and heterogeneous chemistry. The AS-TDMA was installed at a site in eastern Tennessee on the border of Great Smoky Mountains National Park for projects during the summer of 2006 and winter of 2007–2008. During the summer, 12% of the aerosols sampled in continuous AS-TDMA measurements were found to posses two possible hydration states under ambient conditions. In every case, the more hydrated of the possible states was occupied. The remaining 88% did not posses multiple possible states. In continuous measurements during the winter, 49% of the aerosols sampled possessed two possible ambient hydration states; the remainder possessed only one. Of those aerosols with multiple possible ambient hydration states, 65% occupied the more hydrated state; 35% occupied the less hydrated state. This seasonal contrast is supported by differences in the fine particulate (PM<sub>2.5</sub>) composition and ambient RH as measured during the two study periods. In addition to seasonal summaries, this work includes case studies depicting the variation of hydration state with changing atmospheric conditions

Nonlinear optical properties of selected natural pigments extracted from spinach: Carotenoids

They are report here, for the first time in authors knowledge, results on third order nonlinear optical susceptibilities from a series of natural pigments extracted from spinach. The measurements were performed in-situ at 532 nm wavelength using degenerate four wave mixing technique (DFWM). For comparison third order nonlinear optical susceptibilities of the same pigments were also evaluated using third harmonic generation (THG) set up at 1064 nm. The electronic contribution to the observed properties was also deduced. The measurements were performed on thin films deposited on a thick glass substrate. These pigments were also identified by UV–VIS spectral analysis. All these results were in good agreement with the literature data

Effect of zinc nanoparticles on embryo and chicken growth, and the content of zinc in tissues and faeces

The hypothesis was that owing to their high bioavailability, zinc oxide nanoparticles (NanoZnO) can effectively replace (Zn) salts and reduce Zn excretion with faeces. The objective of this study was to investigate the effects of NanoZnO on the development of chicken embryos, the growth of broiler chickens, and Zn excretion with faeces. At day 1 of incubation, 120 eggs were randomly divided between a control group (not injected) and groups injected with a hydrocolloid of NanoZnO in increasing concentrations (50, 100, 500 mg/L). At day 19 of incubation, no differences were observed in the bodyweight, but 100 and 500 mg/L affected liver and heart weights, indicating that high levels of NanoZnO may induce differential organ development. In the subsequent experiment, 308 chickens were randomly divided into six groups. The control diet was supplemented with 55 mg Zn/kg (standard level), the 0 group received no Zn supplement, and groups fed NanoZnO received 25%, 50%, 75%, and 100% of the standard level. The 100% replacement of ZnO with NanoZnO increased the chickens’ bodyweight compared with the standard level of ZnO, but to the same level as the diet without ZnO supplementation. Furthermore, NanoZnO did not reduce the content of Zn in faeces, which was only significantly lower in the group without ZnO supplementation in comparison with other groups. The results indicate that the replacement of ZnO with NanoZnO had no negative effects on chicken growth. Compared with ZnO, NanoZnO did not reduce Zn excretion with faeces. Keywords: broiler, development, excretion, mineral, nanonutritio

The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes

DNA double-strand breaks (DSBs) are toxic to mammalian cells. However, during meiosis, more than 200 DSBs are generated deliberately, to ensure reciprocal recombination and orderly segregation of homologous chromosomes. If left unrepaired, meiotic DSBs can cause aneuploidy in gametes and compromise viability in offspring. Oocytes in which DSBs persist are therefore eliminated by the DNA-damage checkpoint. Here we show that the DNA-damage checkpoint eliminates oocytes via the pro-apoptotic BCL-2 pathway members Puma, Noxa and Bax. Deletion of these factors prevents oocyte elimination in recombination-repair mutants, even when the abundance of unresolved DSBs is high. Remarkably, surviving oocytes can extrude a polar body and be fertilised, despite chaotic chromosome segregation at the first meiotic division. Our findings raise the possibility that allelic variants of the BCL-2 pathway could influence the risk of embryonic aneuploidy

Clinical significance of morphological variations of the inferior phrenic arteries

The rapid development of sciences such as genetics and molecular biology offers hope that better therapeutic methods can be developed and diagnosis and treatment made more effective. However, we must not forget that the basis for understanding the complex mechanisms of diseases and associated symptoms is knowledge of the relevant location and correlation among organs. In the present study, we focus on the clinical significance of the inferior phrenic artery. The diaphragm is a muscular structure that separatesthe abdominal and chest cavities. Thanks to this position, the inferior phrenic artery is much more significant than formerly assumed. A rich network of collaterals makes this vessel important in the development of neoplasms and metastases. Knowledge of anatomical variants of the inferior phrenic artery is also crucial for radiological procedures such as embolization. The main aim of this study is to review the involvement of the inferior phrenic artery in physiological and pathophysiological processes. This work has value for all practicing doctors, especially radiologists and surgeons

Five-headed superior omohyoid

The omohyoid is an infrahyoid muscle with two bellies. It is responsible for lowering and positioning of the hyoid bone. It is morphologically variable in the origin, insertion and morphology of its bellies. Quantitative variations of the superior belly of the omohyoid muscle are not common. We present a case of a five-headed superior omohyoid, and a short clinical review related to this muscle. All the bellies had their origin in an intermediate tendon and were attached to the hyoid bone. The volume of its superior part was greater than usual. Knowledge of the anatomy of this muscle is important, especially for surgeons operating in the anterolateral neck region

Variant origin of three main coronary ostia from the right sinus of Valsalva: report of a rare case

Observing anomalies in the origin of the coronary arteries is a rare but recognized scenario during coronarography. All the major coronary arteries originating from the right sinus of Valsalva is an extremely rare anomaly, its reported incidence being 0.008% in angiographic studies. Most coronary artery variations are benign and are therefore found accidentally or postmortem. However, some anomalies in the origin of the coronary arteries are associated with myocardial ischemia and a higher risk of sudden cardiac death. Herein, we report a sporadic case of anomalous origin of the coronary arteries, in which the right coronary artery, anterior interventricular artery and left circumflex artery arise separately from the right sinus of Valsalva, each originating from a separate ostium. Regardless of their low incidence rate, coronary artery anomalies can cause serious technical challenges during coronary angiography and percutaneous interventions because of the unusual location and course of the artery. Echocardiography, computed tomography, and magnetic resonance imaging can be useful in such cases

Perillaldehyde 1,2-epoxide loaded SLN-tailored mAb: production, physicochemical characterization and in vitro cytotoxicity profile in MCF-7 cell lines

We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer–Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN.The authors wish to acknowledge the financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and from FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe). E.B. Souto acknowledges the sponsorship of the projects M-ERA-NET-0004/2015-PAIRED and UIDB/04469/2020, receiving support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020.info:eu-repo/semantics/publishedVersio

Sensitivity of intermediate mass fragment flows to the symmetry energy

The NIMROD-ISiS array was used to study the transverse flow of intermediate mass fragments in 35 MeV/nucleon ${}^{70}\mathrm{Zn}+{}^{70}\mathrm{Zn}$, ${}^{64}\mathrm{Zn}+{}^{64}\mathrm{Zn}$, and ${}^{64}\mathrm{Ni}+{}^{64}\mathrm{Ni}$ reactions. The intermediate mass fragment flow was previously shown to be sensitive to the density dependence of the symmetry energy. To explore the model dependence of the results, the antisymmetrized molecular dynamics, constrained molecular dynamics, and stochastic mean-field models were each compared to the experimental results to extract information on the form of the symmetry energy. The results demonstrate that sensitivity of the models to the nuclear equation of state can vary significantly based on the treatment of the nuclear dynamics. Despite the differences in the sensitivity, improved agreement with the experimental data is observed for each model with a stiff density dependence of the symmetry energy