403 research outputs found
Heat-induced formation of one-dimensional coordination polymers on Au(111): an STM study
The formation of one-dimensional coordination polymers of cyano- substituted porphyrin derivatives on Au(111) induced by thermal annealing is demonstrated by means of scanning tunnelling microscopy. The polymer is stabilised by an unusual threefold coordination motif mediated between an Au atom and the cyano groups of the porphyrin derivatives.ISSN:1359-7345ISSN:1364-548
Assessing Spatiotemporal Drought Dynamics and Its Related Environmental Issues in the Mekong River Delta
Drought is a major natural disaster that creates a negative impact on socio-economic development and environment. Drought indices are typically applied to characterize drought events in a meaningful way. This study aims at examining variations in agricultural drought severity based on the relationship between standardized ratio of actual and potential evapotranspiration (ET and PET), enhanced vegetation index (EVI), and land surface temperature (LST) derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) platform. A new drought index, called the enhanced drought severity index (EDSI), was developed by applying spatiotemporal regression methods and time-series biophysical data derived from remote sensing. In addition, time-series trend analysis in the 2001â2018 period, along with the MannâKendal (MK) significance test and the Theil Sen (TS) slope, were used to examine the spatiotemporal dynamics of environmental parameters (i.e., LST, EVI, ET, and PET), and geographically weighted regression (GWR) was subsequently applied in order to analyze the local correlations among them. Results showed that a significant correlation was discovered among LST, EVI, ET, and PET, as well as their standardized ratios (|r| > 0.8, p 0.7 and a statistical significance p < 0.01. Besides, it was found that the temporal tendency of this phenomenon was the increase in intensity of drought, and that coastal areas in the study area were more vulnerable to this phenomenon. This study demonstrates the effectiveness of EDSI and the potential application of integrating spatial regression and time-series data for assessing regional drought conditions
Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response
Schult F, Lee TN, Albersmeier A, et al. Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response. NUCLEIC ACIDS RESEARCH. 2018;46(14):7179-7192.Exposure to UV light can result in severe DNA damage. The alternative general transcription factor (GTF) TFB3 has been proposed to play a key role in the UV stress response in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. Reporter gene assays confirmed that tfb3 is upregulated 90-180 min after UV treatment. In vivo tagging and immunodetection of TFB3 confirmed the induced expression at 90 min. Analysis of a tfb3 insertion mutant showed that genes encoding proteins of the Ups pili and the Ced DNA importer are no longer induced in a tfb3 insertion mutant after UV treatment, which was confirmed by aggregation assays. Thus, TFB3 plays a crucial role in the activation of these genes. Genome wide transcriptome analysis allowed a differentiation between a TFB3-dependent and a TFB3-independent early UV response. The TFB3-dependent UV response is characterized by the early induction of TFB3, followed by TFB3-dependent expression of genes involved in e.g. Ups pili formation and the Ced DNA importer. Many genes were downregulated in the tfb3 insertion mutant confirming the hypothesis that TFB3 acts as an activator of transcription. The TFB3-independent UV response includes the repression of nucleotide metabolism, replication and cell cycle progression in order to allow DNA repair
Topological Constraint Theory Analysis of Rigidity Transition in Highly Coordinate Amorphous Hydrogenated Boron Carbide
Topological constraint theory (TCT) has revealed itself to be a powerful tool in interpreting the behaviors of amorphous solids. The theory predicts a transition between a ârigidâ overconstrained network and a âfloppyâ underconstrained network as a function of connectivity or average coordination number, â©râȘ. The predicted results have been shown experimentally for various glassy materials, the majority of these being based on 4-fold-coordinate networks such as chalcogenide and oxide glasses. Here, we demonstrate the broader applicability of topological constraint theory to uniquely coordinated amorphous hydrogenated boron carbide (a-BC:H), based on 6-fold-coordinate boron atoms arranged into partially hydrogenated interconnected 12-vertex icosahedra. We have produced a substantial set of plasma-enhanced chemical vapor deposited a-BC:H films with a large range of densities and network coordination, and demonstrate a clear threshold in Young\u27s modulus as a function of â©râȘ, ascribed to a rigidity transition. We investigate constraint counting strategies in this material and show that by treating icosahedra as âsuperatoms,â a rigidity transition is observed within the range of the theoretically predicted â©râȘc value of 2.4 for covalent solids with bond-stretching and bond-bending forces. This experimental data set for a-BC:H is unique in that it represents a uniform change in connectivity with â©râȘ and demonstrates a distinct rigidity transition with data points both above and below the transition threshold. Finally, we discuss how TCT can be applied to explain and optimize mechanical and dielectric properties in a-BC:H and related materials in the context of microelectronics applications
Recent developments in Tuberculous meningitis pathogenesis and diagnostics [version 2; peer review: 1 approved]
The pathogenesis of Tuberculous meningitis (TBM) is poorly understood, but contemporary molecular biology technologies have allowed for recent improvements in our understanding of TBM. For instance, neutrophils appear to play a significant role in the immunopathogenesis of TBM, and either a paucity or an excess of inflammation can be detrimental in TBM. Further, severity of HIV-associated immunosuppression is an important determinant of inflammatory response; patients with the advanced immunosuppression (CD4+ T-cell count of 150 cells/ÎŒL. Host genetics may also influence outcomes with LT4AH genotype predicting inflammatory phenotype, steroid responsiveness and survival in Vietnamese adults with TBM. Whist in Indonesia, CSF tryptophan level was a predictor of survival, suggesting tryptophan metabolism may be important in TBM pathogenesis. These varying responses mean that we must consider whether a âone-size-fits-allâ approach to anti-bacillary or immunomodulatory treatment in TBM is truly the best way forward. Of course, to allow for proper treatment, early and rapid diagnosis of TBM must occur. Diagnosis has always been a challenge but the field of TB diagnosis is evolving, with sensitivities of at least 70% now possible in less than two hours with GeneXpert MTB/Rif Ultra. In addition, advanced molecular techniques such as CRISPR-MTB and metagenomic next generation sequencing may hold promise for TBM diagnosis. Host-based biomarkers and signatures are being further evaluated in childhood and adult TBM as adjunctive biomarkers as even with improved molecular assays, cases are still missed. A better grasp of host and pathogen behaviour may lead to improved diagnostics, targeted immunotherapy, and possibly biomarker-based, patient-specific treatment regimens
Host directed therapies for tuberculous meningitis [version 2; peer review: 1 approved, 1 approved with reservations]
A dysregulated host immune response significantly contributes to morbidity and mortality in tuberculous meningitis (TBM). Effective host directed therapies (HDTs) are critical to improve survival and clinical outcomes. Currently only one HDT, dexamethasone, is proven to improve mortality. However, there is no evidence dexamethasone reduces morbidity, how it reduces mortality is uncertain, and it has no proven benefit in HIV co-infected individuals. Further research on these aspects of its use, as well as alternative HDTs such as aspirin, thalidomide and other immunomodulatory drugs is needed. Based on new knowledge from pathogenesis studies, repurposed therapeutics which act upon small molecule drug targets may also have a role in TBM. Here we review existing literature investigating HDTs in TBM, and propose new rationale for the use of novel and repurposed drugs. We also discuss host variable responses and evidence to support a personalised approach to HDTs in TBM.</ns3:p
CarbonâEnriched Amorphous Hydrogenated Boron Carbide Films for VeryâLowâk Interlayer Dielectrics
A longstanding challenge in ultralargeâscale integration has been the continued improvement in lowâdielectricâconstant (lowâk) interlayer dielectric materials and other specialized layers in backâendâofâtheâline interconnect fabrication. Modeled after the success of carbonâcontaining organosilicate materials, carbonâenriched amorphous hydrogenated boron carbide (aâBxC:Hy) films are grown by plasmaâenhanced chemical vapor deposition from orthoâcarborane and methane. These films contain more extraicosahedral sp3 hydrocarbon groups than nonenriched aâBxC:Hy films, as revealed by FTIR and NMR spectroscopy, and also exhibit lower dielectric constants than their nonenriched counterparts, notably due to low densities combined with a low distortion and orientation contribution to the total polarizability. Films with dielectric constant as low as 2.5 are reported with excellent electrical stability (leakage current of 10â9 A cmâ2 at 2 MV cmâ1 and breakdown voltage of >6 MV cmâ1), good thermal conductivity of 0.31 ± 0.03 W mâ1 Kâ1, and high projected Youngâs modulus of 12 ± 3 GPa. These properties rival those of leading SiOC:H materials, and position aâBxC:Hy as an important complement to traditional Siâbased materials to meet the complex needs of nextâgeneration interconnect fabrication.Carbonâenriched amorphous hydrogenated boron carbide films are demonstrated with dielectric constant (k) as low as 2.5âattributed to low densities combined with networkârigidifying CH2 bridging groupsâas well as excellent electrical, thermal, and mechanical properties, rivaling those of stateâofâtheâart siliconâbased lowâk dielectric materials.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141869/1/aelm201700116_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141869/2/aelm201700116.pd
Sources of Multidrug Resistance in Patients With Previous Isoniazid-Resistant Tuberculosis Identified Using Whole Genome Sequencing: A Longitudinal Cohort Study
Background
Meta-analysis of patients with isoniazid-resistant tuberculosis given standard first-line anti-tuberculosis treatment indicated an increased risk of multi-drug resistant tuberculosis (MDR-TB) emerging (8%), compared to drug-sensitive tuberculosis (0.3%). Here we use whole genome sequencing (WGS) to investigate whether treatment of patients with pre-existing isoniazid resistant disease with first-line anti-tuberculosis therapy risks selecting for rifampicin resistance, and hence MDR-TB.
Methods
Patients with isoniazid-resistant pulmonary TB were recruited and followed up for 24 months. Drug-susceptibility testing was performed by Microscopic observation drug-susceptibility assay (MODS), Mycobacterial Growth Indicator Tube (MGIT) and by WGS on isolates at first presentation and in the case of re-presentation. Where MDR-TB was diagnosed, WGS was used to determine the genomic relatedness between initial and subsequent isolates. De novo emergence of MDR-TB was assumed where the genomic distance was five or fewer single nucleotide polymorphisms (SNPs) whereas reinfection with a different MDR-TB strain was assumed where the distance was 10 or more SNPs.
Results
239 patients with isoniazid-resistant pulmonary tuberculosis were recruited. Fourteen (14/239, 5.9%) patients were diagnosed with a second episode of tuberculosis that was multi-drug resistant. Six (6/239, 2.5%) were identified as having evolved MDR-TB de novo and six as having been re-infected with a different strain. In two cases the genomic distance was between 5-10 SNPs and therefore indeterminate.
Conclusions
In isoniazid-resistant TB, de novo emergence and reinfection of MDR-TB strains equally contributed to MDR development. Early diagnosis and optimal treatment of isoniazid resistant TB are urgently needed to avert the de novo emergence of MDR-TB during treatment
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