Altered expression of the lymphocyte activation antigen CD30 in active celiac disease

11 páginas.-- et al.Interleukin (IL)-15 and CD30 may be associated with the ongoing intestinal immunologic activation in celiac disease (CD). We studied duodenal biopsies and blood samples of patients with active CD (Cel) and controls in order to determine the regulatory role proposed for CD30+ T cells in this Th1-driven disease and the potential influences of IL-15 on CD30 expression. We detected that a CD30+ T-cell subpopulation persists longer in Cel after a 5 day incubation with anti-CD3 antibody than in controls (p = 0.0063). CD30 upregulation by IL-15 in T blasts was greater in Cel than in controls (p = 0.0062). At the mucosal compartment, the CD30 antigen was examined by immunohistochemistry and quantified on isolated lamina propria (LP) and epithelial T cells by flow cytometry. For Cel and controls, similar mean percentages of CD3+CD30+ intraepithelial T cells (5.88 vs. 5.51, p = ns) and LP T cells (7.38 vs. 7.49, p = ns) were observed at baseline and after in vitro gliadin challenge of duodenal biopsy samples. Our study demonstrates the occurrence of potentially important alterations of the immune response at the peripheral compartment. Our findings also allow us to speculate that a negative effect of soluble mediators at the mucosal compartment might counteract the latent influence of IL-15 on CD30 expression precluding a more severe course of active CD.Peer reviewe

Liver infiltrating mononuclear cells in children with type 1 autoimmune hepatitis

OBJECTIVE: To investigate infiltrating cells in the liver of children with type 1 autoimmune hepatitis (AH‐1). METHODS: liver biopsies from 24 untreated AH‐1 patients (14 children, 10 adults), five patients with hepatitis C virus related chronic hepatitis (HCV), and 10 control liver specimens (CL) were processed for immunohistochemical cell characterisation. RESULTS: Two different cell distribution patterns were detected in the liver of patients with AH‐1: (1) CD4(+) and CD20(+) cells were found in the central areas of the portal tracts (portal distribution); (2) CD8(+) cells were observed at the periphery of the portal space (periportal distribution). Some cell subsets, like CD56, CD57, Fas‐L, and Bak, showed a non‐defined distribution pattern. The presence of two well defined patterns of cell distribution was not observed in HCV and CL (CD4(+), CD20(+), and CD8(+) cells were uniformly distributed in the portal space). In AH‐1 and CL, the NK markers CD56 and CD57 were found scattered throughout the liver parenchyma. However, in HCV biopsies, CD56(+) cells were also clearly increased in both the portal and the periportal areas. Biopsies of AH‐1 and HCV patients showed a uniform distribution of Fas‐L and Bak in the portal and periportal areas, with Bak staining also detected in the hepatic parenchyma. CONCLUSIONS: Despite clinical and genetic differences, there was a similar distribution of liver infiltrating mononuclear cells in children and adults with AH‐1. These results raise the possibility of reclassifying cryptogenic chronic hepatitis by immunohistochemical analysis of infiltrating liver cells

Protocolo de uso de "enoxaparina" en el HNC

3 p. Documento elaborado por la Farmacia Central del Hospital Nacional de ClínicasFil: Bustos Fierro, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Hospital Nacional de Clínicas. Farmacia Central; Argentina.Se entiende por MEDICAMENTO DE USO RESTRINGIDO a aquel para el que, mediante un procedimiento participativo, multidisciplinar y representativo del hospital, su uso ha sido restringido a determinados grupos de pacientes o a determinadas situaciones clínicas para asegurar una mayor eficacia, evitar efectos adversos, por motivos epidemiológicos o por motivos económicos. El Hospital Nacional de Clínicas (HNC) cuenta con un listado de medicamentos de uso restringido entre los que se encuentra la ENOXAPARINA (EX), corresponde a una Heparina de Bajo Peso Molecular. La EX es un agente antitrombótico que inhibe la coagulación potenciando el efecto inhibitorio de la antitrombina III sobre los factores IIa y Xa. Posee elevada actividad anti-Xa y débil actividad anti-IIa.info:eu-repo/semantics/acceptedVersionFil: Bustos Fierro, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Hospital Nacional de Clínicas. Farmacia Central; Argentina

Versión 2

6 p. Versión 2Fil: Bustos Fierro, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Hospital Nacional de Clínicas. Farmacia Central; Argentina.Se entiende por PRODUCTO MÉDICO (PM) de uso restringido a aquel para el que, mediante un procedimiento participativo, multidisciplinar y representativo del hospital, su uso ha sido restringido a determinados grupos de pacientes o a determinadas situaciones clínicas por motivos epidemiológicos, económicos o para evitar complicaciones. El Hospital Nacional de Clínicas (HNC) cuenta con PM de uso restringido como es el CATETER VENOSO CENTRAL (CVC).info:eu-repo/semantics/acceptedVersionFil: Bustos Fierro, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Hospital Nacional de Clínicas. Farmacia Central; Argentina

Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity

Background: During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives: The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design: This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results: Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions: Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.Fil: Periolo, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Avaro, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Czech, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Russo, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Benedetti, E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Pontoriero, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Campos, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Martinez Peralta, Liliana A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Baumeister, Elsa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentin