Genome-wide microRNA screening in Nile tilapia reveals pervasive isomiRs’ transcription, sex-biased arm switching and increasing complexity of expression throughout development

MicroRNAs (miRNAs) are key regulators of gene expression in multicellular organisms. The elucidation of miRNA function and evolution depends on the identification and characterization of miRNA repertoire of strategic organisms, as the fast-evolving cichlid fishes. Using RNA-seq and comparative genomics we carried out an in-depth report of miRNAs in Nile tilapia (Oreochromis niloticus), an emergent model organism to investigate evo-devo mechanisms. Five hundred known miRNAs and almost one hundred putative novel vertebrate miRNAs have been identified, many of which seem to be teleost-specific, cichlid-specific or tilapia-specific. Abundant miRNA isoforms (isomiRs) were identified with modifications in both 5p and 3p miRNA transcripts. Changes in arm usage (arm switching) of nine miRNAs were detected in early development, adult stage and even between male and female samples. We found an increasing complexity of miRNA expression during ontogenetic development, revealing a remarkable synchronism between the rate of new miRNAs recruitment and morphological changes. Overall, our results enlarge vertebrate miRNA collection and reveal a notable differential ratio of miRNA arms and isoforms influenced by sex and developmental life stage, providing a better picture of the evolutionary and spatiotemporal dynamics of miRNAs

- Kant-Index, Band 30, Teilband I

Il primo volume della prima edizione critica attendibile del Naturrecht Feyerabend, il manoscritto relativo ad un corso di lezioni sul diritto naturale tenuto da Kant nel 1784. Si tratta dell’unica trascrizione manoscritta da noi posseduta di un corso di Kant sull’argomento, a cura di uno studente di nome appunto Feyerabend. Essa era stata edita nel vol. XXVII, 2,2 dell’edizione dell’Accademia delle opere kantiane (Berlin, 1979), ma con una trascrizione rivelatasi molto imprecisa. L’edizione è prevista in due volumi. L’edizione critica del testo tedesco (rivista sul manoscritto) è accompagnata da note, da indici e concordanze, dall’indicazione dei paralleli con la coeva Fondazione della metafisica dei costumi di Kant.[...

Let the play set come alive: Supporting playful learning through the digital augmentation of a traditional toy environment

The Augmented Knight's Castle (AKC) comprises traditional play figures and scenery embedded with radio frequency identification (RFID) technology to enhance user experience by triggering various forms of audio output. In this paper we present the results of a user study with over 100 children to evaluate the AKC in terms of playful learning, compared with an identical, non-augmented version. Findings suggest that children who played with the AKC remembered facts about the Middle Ages that were presented to them in the form of verbal commentaries, both immediately after the play session as well as in a post-test two months later. 2010 IEEE