State of the art in bile analysis in forensic toxicology

AbstractIn forensic toxicology, alternative matrices to blood are useful in case of limited, unavailable or unusable blood sample, suspected postmortem redistribution or long drug intake-to-sampling interval. The present article provides an update on the state of knowledge for the use of bile in forensic toxicology, through a review of the Medline literature from 1970 to May 2015. Bile physiology and technical aspects of analysis (sampling, storage, sample preparation and analytical methods) are reported, to highlight specificities and consequences from an analytical and interpretative point of view. A table summarizes cause of death and quantification in bile and blood of 133 compounds from more than 200 case reports, providing a useful tool for forensic physicians and toxicologists involved in interpreting bile analysis. Qualitative and quantitative interpretation is discussed. As bile/blood concentration ratios are high for numerous molecules or metabolites, bile is a matrix of choice for screening when blood concentrations are low or non-detectable: e.g., cases of weak exposure or long intake-to-death interval. Quantitative applications have been little investigated, but small molecules with low bile/blood concentration ratios seem to be good candidates for quantitative bile-based interpretation. Further experimental data on the mechanism and properties of biliary extraction of xenobiotics of forensic interest are required to improve quantitative interpretation

Transesophageal echocardiographic evaluation of baboons during microgravity induced by parabolic flight

Transthoracic echocardiography (TTE) is a feasible method to noninvasively examine cardiac anatomy during parabolic flight. However, transducer placement on the chest wall is very difficult to maintain during transition to microgravity. In addition, TTE requires the use of low frequency transponders which limit resolution. Transesophical echocardiography (TEE) is an established imaging technique which obtains echocardiographic information from the esophagus. It is a safe procedure and provides higher quality images of cardiac structure than obtained with TTE. This study is designed to determine whether TEE was feasible to perform during parabolic flight and to determine whether acute central volume responses occur in acute transition to zero gravity by direct visualization of the cardiac chambers

Global retrieval of phytoplankton functional types based on empirical orthogonal functions using CMEMS GlobColour merged products and further extension to OLCI data

This study presents an algorithm for globally retrieving chlorophyll a (Chl-a) concentrations of phytoplankton functional types (PFTs) from multi-sensor merged ocean color (OC) products or Sentinel-3A (S3) Ocean and Land Color Instrument (OLCI) data from the GlobColour archive in the frame of the Copernicus Marine Environmental Monitoring Service (CMEMS). The retrieved PFTs include diatoms, haptophytes, dinoflagellates, green algae and prokaryotic phytoplankton. A previously proposed method to retrieve various phytoplankton pigments, based on empirical orthogonal functions (EOF), is investigated and adapted to retrieve Chl-a concentrations of multiple PFTs using extensive global data sets of in situ pigment measurements and matchups with satellite OC products. The performance of the EOF-based approach is assessed and cross-validated statistically. The retrieved PFTs are compared with those derived from diagnostic pigment analysis (DPA) based on in situ pigment measurements. Results show that the approach predicts well Chl-a concentrations of most of the mentioned PFTs. The performance of the approach is, however, less accurate for prokaryotes, possibly due to their general low variability and small concentration range resulting in a weak signal which is extracted from the reflectance data and corresponding EOF modes. As a demonstration of the approach utilization, the EOF-based fitted models based on satellite reflectance products at nine bands are applied to the monthly GlobColour merged products. Climatological characteristics of the PFTs are also evaluated based on ten years of merged products (2002−2012) through inter-comparisons with other existing satellite derived products on phytoplankton composition including phytoplankton size class (PSC), SynSenPFT, OC-PFT and PHYSAT. Inter-comparisons indicate that most PFTs retrieved by our study agree well with previous corresponding PFT/PSC products, except that prokaryotes show higher Chl-a concentration in low latitudes. PFT dominance derived from our products is in general well consistent with the PHYSAT product. A preliminary experiment of the retrieval algorithm using eleven OLCI bands is applied to monthly OLCI products, showing comparable PFT distributions with those from the merged products, though the matchup data for OLCI are limited both in number and coverage. This study is to ultimately deliver satellite global PFT products for long-term continuous observation, which will be updated timely with upcoming OC data, for a comprehensive understanding of the variability of phytoplankton composition structure at a global or regional scale

Thermocapillary actuation of liquid flow on chemically patterned surfaces

We have investigated the thermocapillary flow of a Newtonian liquid on hydrophilic microstripes which are lithographically defined on a hydrophobic surface. The speed of the microstreams is studied as a function of the stripe width w, the applied thermal gradient |dT/dx| and the liquid volume V deposited on a connecting reservoir pad. Numerical solutions of the flow speed as a function of downstream position show excellent agreement with experiment. The only adjustable parameter is the inlet film height, which is controlled by the ratio of the reservoir pressure to the shear stress applied to the liquid stream. In the limiting cases where this ratio is either much smaller or much larger than unity, the rivulet speed shows a power law dependency on w, |dT/dx| and V. In this study we demonstrate that thermocapillary driven flow on chemically patterned surfaces can provide an elegant and tunable method for the transport of ultrasmall liquid volumes in emerging microfluidic technologies

Broad iron lines in Active Galactic Nuclei

An intrinsically narrow line emitted by an accretion disk around a black hole appears broadened and skewed as a result of the Doppler effect and gravitational redshift. The fluorescent iron line in the X-ray band at 6.4-6.9keV is the strongest such line and is seen in the X-ray spectrum of many active galactic nuclei and, in particular, Seyfert galaxies. It is an important diagnostic with which to study the geometry and other properties of the accretion flow very close to the central black hole. The broad iron line indicates the presence of a standard thin accretion disk in those objects, often seen at low inclination. The broad iron line has opened up strong gravitational effects around black holes to observational study with wide-reaching consequences for both astrophysics and physics.Comment: 26 pages. Invited review, accepted for publication in PAS

A global climatology of the mesospheric sodium layerfrom GOMOS data during the 2002-2008 period

This paper presents a climatology of the mesospheric sodium layer built from the processing of 7 years of GOMOS data. With respect to preliminary results already published for the year 2003, a more careful analysis was applied to the averaging of occultations inside the climatological bins (10° in latitude-1 month). Also, the slant path absorption lines of the Na doublet around 589 nm shows evidence of partial saturation that was responsible for an underestimation of the Na concentration in our previous results. The sodium climatology has been validated with respect to the Fort Collins lidar measurements and, to a lesser extent, to the OSIRIS 2003–2004 data. Despite the important natural sodium variability, we have shown that the Na vertical column has a marked semi-annual oscillation at low latitudes that merges into an annual oscillation in the polar regions, a spatial distribution pattern that was unreported so far. The sodium layer seems to be clearly influenced by the mesospheric global circulation and the altitude of the layer shows clear signs of subsidence during polar winter. The climatology has been parameterized by time-latitude robust fits to allow for easy use. Taking into account the non-linearity of the transmittance due to partial saturation, an experimental approach is proposed to derive mesospheric temperatures from limb remote sounding measurements

Global Retrieval Algorithms for Phytoplankton Functional Types (PFTs): toward the Applications to OLCI and GlobColour Merged Products

With integrated use of extensive in situ measurements from various cruises in different regions, this study focuses on PFT retrieval algorithms that are then applied to Sentinel-3 (S3) OLCI data and merged ocean colour (OC) products from CMEMS GlobColour archive. The main retrieved PFTs include diatoms, haptophytes, prokaryotic phytoplankton (cyanobacteria). Previously investigated retrieval methods, empirical orthogonal functions (EOF) for pigment concentrations estimation (Bracher et al. 2015) and generalized IOP (GIOP) (Werdell et al. 2013, 2014) for PFT discrimination, are tested and adapted potentially with full use of our current available in situ measurements from various campaigns worldwide, in which we have a number of collocated remote sensing reflectance spectra (Rrs) and PFT data based on HPLC pigments in addition to other bio-optical measurements. Algorithms are tested and compared by both taking hyperspectral and multispectral in situ Rrs spectra as input data, and the multispectral based approach is later on applied to the above mentioned satellite data. Performances of both EOF- and GIOP-based approaches are assessed statistically and cross-validated, with results showing that both could well predict chlorophyll-a concentrations for diatoms and haptophytes but less good for prokaryotes. In a next step these algorithms are adapted to satellite OC data collocated to an even larger in-situ PFT database derived from HPLC phytoplankton pigments. This is to eventually develop the global satellite PFT products for long-term observation, updated timely with more available OLCI data in the future, and intercompared to the results with other existing PFT products (e.g. PhytoDOAS, OC-PFT, SynSenPFT)

Multi-Temperature Blackbody Spectrum of a Thin Accretion Disk around a Kerr Black Hole: Model Computations and Comparison with Observations

We use a ray-tracing technique to compute the observed spectrum of a thin accretion disk around a Kerr black hole. We include all relativistic effects such as frame-dragging, Doppler boost, gravitational redshift, and bending of light by the gravity of the black hole. We also include self-irradiation of the disk as a result of light deflection. Assuming that the disk emission is locally blackbody, we show how the observed spectrum depends on the spin of the black hole, the inclination of the disk, and the torque at the inner edge of the disk. We find that the effect of a nonzero torque on the spectrum can, to a good approximation, be absorbed into a zero-torque model by adjusting the mass accretion rate and the normalization. We describe a computer model, called KERRBB, which we have developed for fitting the spectra of black hole X-ray binaries. Using KERRBB within the X-ray data reduction package XSPEC, and assuming a spectral hardening factor f_col = 1.7, we analyze the spectra of three black hole X-ray binaries: 4U1543-47, XTE J1550-564, and GRO J1655-40. We estimate the spin parameters of the black holes in 4U1543-47 and GRO J1655-40 to be a/M ~ 0.6 and ~ 0.6-0.7, respectively. If f_col ~ 1.5-1.6, as in a recent study, then we find a/M ~ 0.7-0.8 and ~ 0.8-0.9, respectively. These estimates are subject to additional uncertainties in the assumed black hole masses, distances and disk inclinations.Comment: 75 pages, including 14 figures; accepted for publication in ApJS on December 14, 200

Gravitating discs around black holes

Fluid discs and tori around black holes are discussed within different approaches and with the emphasis on the role of disc gravity. First reviewed are the prospects of investigating the gravitational field of a black hole--disc system by analytical solutions of stationary, axially symmetric Einstein's equations. Then, more detailed considerations are focused to middle and outer parts of extended disc-like configurations where relativistic effects are small and the Newtonian description is adequate. Within general relativity, only a static case has been analysed in detail. Results are often very inspiring, however, simplifying assumptions must be imposed: ad hoc profiles of the disc density are commonly assumed and the effects of frame-dragging and completely lacking. Astrophysical discs (e.g. accretion discs in active galactic nuclei) typically extend far beyond the relativistic domain and are fairly diluted. However, self-gravity is still essential for their structure and evolution, as well as for their radiation emission and the impact on the environment around. For example, a nuclear star cluster in a galactic centre may bear various imprints of mutual star--disc interactions, which can be recognised in observational properties, such as the relation between the central mass and stellar velocity dispersion.Comment: Accepted for publication in CQG; high-resolution figures will be available from http://www.iop.org/EJ/journal/CQ

Gravitational Lensing by Black Holes

We review the theoretical aspects of gravitational lensing by black holes, and discuss the perspectives for realistic observations. We will first treat lensing by spherically symmetric black holes, in which the formation of infinite sequences of higher order images emerges in the clearest way. We will then consider the effects of the spin of the black hole, with the formation of giant higher order caustics and multiple images. Finally, we will consider the perspectives for observations of black hole lensing, from the detection of secondary images of stellar sources and spots on the accretion disk to the interpretation of iron K-lines and direct imaging of the shadow of the black hole.Comment: Invited article for the GRG special issue on lensing (P. Jetzer, Y. Mellier and V. Perlick Eds.). 31 pages, 12 figure