Femtosecond Pump-Probe Diagnostics Of Preformed Plasma Channels

We report on recent ultrafast pump-probe experiments 28 in He plasma waveguides using 800 nm, 80 fs pump pulses of 0.2 x 1018 W/cm2 peak guided intensity, and single orthogonally-polarized 800 nm probe pulses with similar to0.1% of pump intensity. The main results are: (1) We observe frequency-domain interference between the probe and a weak, depolarized component of the pump that differs substantially in mode shape from the injected pump pulse; (2) we observe spectral blue-shifts in the transmitted probe that are not evident in the transmitted pump. The evidence indicates that pump depolarization and probe blue-shifts both originate near the channel entrance.Physic

Tracing the first steps of American sturgeon pioneers in Europe

Background: A Baltic population of Atlantic sturgeon was founded ~1,200 years ago by migrants from North America, but after centuries of persistence, the population was extirpated in the 1960s, mainly as a result of over-harvest and habitat alterations. As there are four genetically distinct groups of Atlantic sturgeon inhabiting North American rivers today, we investigated the genetic provenance of the historic Baltic population by ancient DNA analyses using mitochondrial and nuclear markers. Results: The phylogeographic signal obtained from multilocus microsatellite DNA genotypes and mitochondrial DNA control region haplotypes, when compared to existing baseline datasets from extant populations, allowed for the identification of the region-of-origin of the North American Atlantic sturgeon founders. Moreover, statistical and simulation analyses of the multilocus genotypes allowed for the calculation of the effective number of individuals that originally founded the European population of Atlantic sturgeon. Our findings suggest that the Baltic population of A. oxyrinchus descended from a relatively small number of founders originating from the northern extent of the species' range in North America. Conclusion: These results demonstrate that the most northerly distributed North American A. oxyrinchus colonized the Baltic Sea ~1,200 years ago, suggesting that Canadian specimens should be the primary source of broodstock used for restoration in Baltic rivers. This study illustrates the great potential of patterns obtained from ancient DNA to identify population-of-origin to investigate historic genotype structure of extinct populations

Excited States of Proton-bound DNA/RNA Base Homo-dimers: Pyrimidines

We are presenting the electronic photo fragment spectra of the protonated pyrimidine DNA bases homo-dimers. Only the thymine dimer exhibits a well structured vibrational progression, while protonated monomer shows broad vibrational bands. This shows that proton bonding can block some non radiative processes present in the monomer.Comment: We acknowledge the use of the computing facility cluster GMPCS of the LUMAT federation (FR LUMAT 2764

Metaphors in Nanomedicine: The Case of Targeted Drug Delivery

International audienceThe promises of nanotechnology have been framed by a variety of metaphors, that not only channel the attention of the public, orient the questions asked by researchers, and convey epistemic choices closely linked to ethical preferences. In particular, the image of the 'therapeutic missile' commonly used to present targeted drug delivery devices emphasizes precision, control, surveillance and efficiency. Such values are highly praised in the current context of crisis of pharmaceutical innovation where military metaphors foster a general mobilization of resources from multiple fields of cutting-edge research. The missile metaphor, reminiscent of Paul Ehrlich's 'magic bullet', has framed the problem in simple terms: how to deliver the right dose in the right place at the right moment? Chemists, physicists and engineers who design multi-functional devices operating in vitro can think in such terms, as long as the devices are not actually operating through the messy environment of the body. A close look at what has been done and what remains to be done suggests that the metaphor of the "therapeutic missile" is neither sufficient, nor even necessary. Recent developments in nanomedicine suggest that therapeutic efficacy cannot be obtained without negotiating with the biological milieu and taking advantage of what it affords. An 'oïkological' approach seems more appropriate, more heuristic and more promising than the popular missile. It is based on the view of organism as an oikos that has to be carefully managed. The dispositions of nanocapsules have to be coupled with the affordances of the environment. As it requires dealing with nanoparticles as relational entities (defined by their potential for interactions) rather than as stable substances (defined by intrinsic properties) this metaphor eventually might well change research priorities in nanotechnology in general

Genetic analyses of archival specimens of the Atlantic sturgeon Acipenser sturio L., 1758

Se analizó la variabilidad genética en Acipenser sturio L., 1758 y Acipenser oxyrinchus oxyrinchus Mitchill, 1815 usando la variación en la región D-loop del ADN mitocondrial y en un número de microsatélites (marcadores nucleares). El material estudiado incluyó muestras de tejidos de: (1) 38 ejemplares almacenados de A. sturio colectados en diferentes museos de historia natural alemanes, suecos, daneses y franceses; (2) 27 A. sturio vivos correspondientes al stock de cría para recuperación de esta especie en aguas alemanas (Instituto de Ecología Dulceacuícola y Pescas Interiores, Berlín, Alemania); (3) 30 A. o. oxyrinchus silvestres capturados en el océano Atlántico cerca de la costa de Nueva Jersey y en el río Delaware (USA); y (4) 60 individuos de A. o. oxyrinchus de un stock reproducido artificialmente obtenido originalmente de diversos esturiones silvestres capturados en el río San Juan (Canadá). Se clonó y secuenció un fragmento de 250 pares de bases de la región D-loop del ADN mitocondrial. La longitud de una unidad repetida fue de 80 pares de bases en A. sturio y de 79 en A. o. oxyrinchus. Las unidades repetidas de A. sturio y A. o. oxyrinchus difirieron por 11 sustituciones y una delección o inserción, respectivamente. No se encontró heteroplasmia. Se observaron tres diferentes haplotipos de ADN mitocondrial en ambas especies. Cinco microsatélites presentaron patrones polimórficos de bandas. En A. sturio los análisis de microsatélites mostraron una disminución en números alélicos entre los años 1823 y 1992. Este declive tuvo como consecuencia la fijación de varios alelos. Para A. sturio, se observaron un haplotipo de ADN mitocondrial y siete alelos sólo en los ejemplares almacenados. Los cálculos de distancia genética mostraron una gran similitud genética entre las poblaciones del Gironda y del Mar del Norte, y una posición basal de las poblaciones de A. sturio del Mediterráneo y del Adriático. En A. o. oxyrinchus el número de alelos de microsatélite varió entre 14 (ríos Hudson y San Juan) y 22 (río Delaware). Los cálculos de distancia genética mostraron una gran similitud genética entre las subpoblaciones de A. o. oxyrinchus.Genetic variability was analysed in Acipenser sturio L., 1758 and Acipenser oxyrinchus oxyrinchus Mitchill, 1815 using variation in the D-loop region of mtDNA and a number of microsatellites (nuclear markers). The studied material included tissue samples from: (1) 38 A. sturio archival specimens collected in different German, Swedish, Danish, and French museums of natural history; (2) 27 live A. sturio representing a broodstock for restoration of this species in German waters (Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany); (3) 30 wild A. o. oxyrinchus caught in the Atlantic Ocean near the coast of New Jersey and in the Delaware River (USA); and (4) 60 individuals of A. o. oxyrinchus from an artificially reproduced stock originally obtained from several wild sturgeons captured in the St. John River (Canada). A 250-bp fragment of the D-loop region of mtDNA was cloned and sequenced. The length of a repeated unit was 80 bp in A. sturio and 79 bp in A. o. oxyrinchus. The repeated units of A. sturio and A. o. oxyrinchus differed by 11 substitutions and one deletion or insertion, respectively. No heteroplasmy was found. Three different haplotypes of mtDNA were observed in both species. Five microsatellites had polymorphic band patterns. In A. sturio, analyses of microsatellites showed a decrease in allelic numbers between the years 1823 and 1992. This decline resulted in a fixation of several alleles. For A. sturio, one mtDNA haplotype and seven alleles were observed only in archival samples. Genetic distance calculations showed a great genetic similarity between A. sturio populations in the Gironde River and the North Sea, and a basal position of the Mediterranean and Adriatic Sea A. sturio populations. In A. o. oxyrinchus, the number of microsatellite alleles ranged between 14 (Hudson and St. John rivers) and 22 (Delaware River). Genetic distance calculations showed a high genetic similarity between subpopulations of A. o. oxyrinchus.Instituto Español de Oceanografí

iMARS Phase 2

The file attached is the Published/publisher’s pdf version of the articl

Perceived Overqualification and Collectivism Orientation:Implications for Work and Nonwork Outcomes

In this research, we simultaneously examined the relative applicability of person-environment fit and relative deprivation theories in explaining the interactive effects of perceived overqualification and collectivism cultural orientations on positive outcomes. We hypothesized that the negative (positive) influence of perceived overqualification on person-environment fit (relative deprivation) will be weaker among employees with high collectivism cultural orientation. We also examined which of these two different mechanisms would explain the hypothesized interactive effects in predicting these workers’ citizenship behavior, personal initiative, work engagement, and life satisfaction. We tested our hypotheses in two studies. In Study 1, we recruited professional staff (n = 852) and their coworkers (n = 301) from 95 universities and tested our hypotheses in a matched sample of 190 employees and their peers. The moderated mediation results supported the idea of person-environment fit (but not relative deprivation) as the mechanism explaining why collectivism orientations assuaged the negative effects of perceived overqualification on these outcomes. We constructively replicated these results in Study 2, which was a time-lagged design with full-time employees (n = 224). Study 2’s results further supported the robustness of our model by testing alternative moderators, mediators, and outcomes.</p