Characterization of Shewanella oneidensis MtrC: a cell-surface decaheme cytochrome involved in respiratory electron transport to extracellular electron acceptors

MtrC is a decaheme c-type cytochrome associated with the outer cell membrane of Fe(III)-respiring species of the Shewanella genus. It is proposed to play a role in anaerobic respiration by mediating electron transfer to extracellular mineral oxides that can serve as terminal electron acceptors. The present work presents the first spectropotentiometric and voltammetric characterization of MtrC, using protein purified from Shewanella oneidensis MR-1. Potentiometric titrations, monitored by UV–vis absorption and electron paramagnetic resonance (EPR) spectroscopy, reveal that the hemes within MtrC titrate over a broad potential range spanning between approximately +100 and approximately -500 mV (vs. the standard hydrogen electrode). Across this potential window the UV–vis absorption spectra are characteristic of low-spin c-type hemes and the EPR spectra reveal broad, complex features that suggest the presence of magnetically spin-coupled low-spin c-hemes. Non-catalytic protein film voltammetry of MtrC demonstrates reversible electrochemistry over a potential window similar to that disclosed spectroscopically. The voltammetry also allows definition of kinetic properties of MtrC in direct electron exchange with a solid electrode surface and during reduction of a model Fe(III) substrate. Taken together, the data provide quantitative information on the potential domain in which MtrC can operate

Gravel pits support waterbird diversity in an urban landscape

We assessed the benefit of 11 gravel pits for the settlement of waterbird communities in an urbanized area lacking natural wetlands. Gravel pits captured 57% of the regional species pool of aquatic birds. We identified 39 species, among which five were regionally rare. We used the Self Organizing Map algorithm to calculate the probabilities of presence of species, and to bring out habitat conditions that predict assemblage patterns. The age of the pits did not correlate with assemblage composition and species richness. There was a positive influence of macrophyte cover on waterbird species richness. Larger pits did not support more species, but species richness increased with connectivity. As alternative wetland habitats, gravel pits are attractive to waterbirds, when they act as stepping stones that ensure connectivity between larger natural and/or artificial wetlands separated in space

Performance of parental genotypes and inheritance of Angular Leaf Spot (Phaeosariopsis griseola) resistance in the common bean (Phaseolus vulgaris)

Two studies, one on performance of six common bean parental genotypes and another on inheritance of resistance to Phaeosariopsis griseola (Pg) in the common bean were carried out in Malawi. Common bean entries namely; Chimbamba, Nasaka, RC 15, CAL 143 and Mexico 54 were evaluated on station in the 2004/2005 growing season at Bunda, Dedza, Ng'onga and Ntchenachena sites. The second study started by generating F1s and then F2 and F3 seeds in greenhouse at Bunda College. CAL 143 and Mexico 54 were sources of Pg resistance genes while Chimbamba, Nasaka and RC 15 were susceptible recipient parents. Following green house trials, Pg resistance was evaluated on station in the samesites. CAL 143 was highest yielding but unstable across sites. RC 15 was stable and gave the highest yield at the dry-spell-stricken Ng’onga whereas Mexico 54 was superior at Ntchenachena but highly unstable across sites. Yield was strongly correlated to number of effective pods per plant in all genotypes. The inheritance study showed that resistance to Pg in the common bean is controlled by one gene using both CAL 143 and Mexico 54 as resistant parents.&#160

Massive Quantum Liquids from Holographic Angel's Trumpets

We explore the small-temperature regime in the deconfined phase of massive fundamental matter at finite baryon number density coupled to the 3+1 dimensional N=4 SYM theory. In this setting, we can demonstrate a new type of non-trivial temperature-independent scaling solutions for the probe brane embeddings. Focusing mostly on matter supported in 2+1 dimensions, the thermodynamics indicate that there is a quantum liquid with interesting density-dependent low-temperature physics. We also comment about 3+1 and 1+1 dimensional systems, where we further find for example a new thermodynamic instability.Comment: 18+1 pages, 6 figures; replaced fig. 6 and comments in sec. 5.2; minor explanations added and typos fixed, final version published in JHEP (modulo fig. 3); factor of \sqrt{\lambda} and corresponding comments fixe

Study of Foaming Properties and Effect of the Isomeric Distribution of Some Anionic Surfactants

Using different reaction conditions of photosulfochlorination of n-dodecane, two samples of anionic surfactants of sulfonate type are obtained. Their micellar behavior has been already reported and the relationship between their isomeric distribution and their chemical structures and micellar behaviors have been more thoroughly explored. In this investigation, we screened the foaming properties (foaming power and foam stability) by a standardized method very similar to the Ross–Miles foaming tests to identify which surfactants are suitable for applications requiring high foaming, or, alternatively, low foaming. The results obtained for the synthesized surfactants are compared to those obtained for an industrial sample of secondary alkanesulfonate (Hostapur 60) and to those of a commercial sample of sodium dodecylsulfate used as reference for anionic surfactants. The foam formation and foam stability of aqueous solutions of the two samples of dodecanesulfonate are compared as a function of their isomeric distribution. These compounds show good foaming power characterized in most cases by metastable or dry foams. The highest foaming power is obtained for the sample rich in primary isomers which also produces foam with a relatively high stability. For the sample rich in secondary isomers we observe under fixed conditions a comparable initial foam height but the foam stability turns out to be low. This property is interesting for applications requiring low foaming properties such as dishwashing liquid for machines. The best results are observed near and above the critical micellar concentrations and at 25 C for both the samples

Meson Thermalization in Various Dimensions

In gauge/gravity duality framework the thermalization of mesons in strongly coupled (p+1)-dimensional gauge theories is studied for a general Dp-Dq system, q>=p, using the flavour Dq-brane as a probe. Thermalization corresponds to the horizon formation on the flavour Dq-brane. We calculate the thermalization time-scale due to a time-dependent change in the baryon number chemical potential, baryon injection in the field theory. We observe that for such a general system it has a universal behaviour depending only on the t'Hooft coupling constant and the two parameters which describe how we inject baryons into the system. We show that this universal behaviour is independent of the details of the theory whether it is conformal and/or supersymmetric.Comment: 26 pages, 2 figure

Corner contributions to holographic entanglement entropy

The entanglement entropy of three-dimensional conformal field theories contains a universal contribution coming from corners in the entangling surface. We study these contributions in a holographic framework and, in particular, we consider the effects of higher curvature interactions in the bulk gravity theory. We find that for all of our holographic models, the corner contribution is only modified by an overall factor but the functional dependence on the opening angle is not modified by the new gravitational interactions. We also compare the dependence of the corner term on the new gravitational couplings to that for a number of other physical quantities, and we show that the ratio of the corner contribution over the central charge appearing in the two-point function of the stress tensor is a universal function for all of the holographic theories studied here. Comparing this holographic result to the analogous functions for free CFT's, we find fairly good agreement across the full range of the opening angle. However, there is a precise match in the limit where the entangling surface becomes smooth, i.e., the angle approaches $\pi$, and we conjecture the corresponding ratio is a universal constant for all three-dimensional conformal field theories. In this paper, we expand on the holographic calculations in our previous letter arXiv:1505.04804, where this conjecture was first introduced.Comment: 62 pages, 6 figures, 1 table; v2: minor modifications to match published version, typos fixe

Viscosity Bound and Causality in Superfluid Plasma

It was argued by Brigante et.al that the lower bound on the ratio of the shear viscosity to the entropy density in strongly coupled plasma is translated into microcausality violation in the dual gravitational description. Since transport properties of the system characterize its infrared dynamics, while the causality of the theory is determined by its ultraviolet behavior, the viscosity bound/microcausality link should not be applicable to theories that undergo low temperature phase transitions. We present an explicit model of AdS/CFT correspondence that confirms this fact.Comment: 27 pages, 5 figures. References added, typos fixe