Diversity and Taxonomic Identity of Rumen Bacterial Community in Cattle Fed Different Diets

In this study, the identity of rumen bacterial community and their taxonomic classification in Zebu heifers (n=24) fed 4 different diets (range grasses, Bracharia MulatoII, Azolla and Cassava leaf meal) was surveyed using metagenomics sequencing of the 16SrRNA gene. Rumen liquor samples were collected from the heifers from which a total of 192 DNA samples were amplified and the resulting 16S rRNA sequences compared to the existing sequences at the National Centre for Biotechnology Information (NCBI) BLAST database through the MetagenAssist . Bioinformatics analyses indicated that 17 operational taxonomic units (OTUs) were present at the phylum level. Of these, 43.3% were affiliated to the phylum Firmicutes, 27.2% Bacteroidetes, 22.8% Proteobacteria and 1.7% Euryarchaeota. The remaining were Cyanobacteria (1.4%), Chloroflexi (1%) while Actinobacteria, Verrucomicrobia, Spirochaetes, Tenericutes, Planctomycetes, Elusimicrobia, Lentisphaerae, Armatimonadetes, Fibrobacteres, Synergistetes and Arthropoda were all below 1%. Both Time and Diet had significant effect on the abundance of microbes but did not affect their diversity. Different diets therefore can affect the abundance of rumen microbiome and eventually the performance of animals

Tomato Management Practices and Diseases Occurrence in Mwea West Sub County

Tomato is an important crop in Mwea West Sub County, Kirinyaga County, Kenya. A survey was carried out in the area to investigate tomato management practices, diseases and pests that hinder tomato production. The study endeavoured to establish farmers’ knowledge on fusarium wilt disease and root-knot nematodes and the methods used to control them. Data was collected from two hundred and eighteen randomly selected small holder producers who were equally distributed in the study area. Data collected included tomato management practices, diseases and pests that hinder production. Majority (85.3%) of the respondents were males while a few (14.7%) were female. The respondents (71.6%) indicated that tomato was the most important crop grown for income generation in the area. Most important varieties grown were cultivars, Safari, Kilele F1, Prosta F1 and Rio- Grande. Most important diseases affecting tomato crop were; early blight (Alternaria solani) and late blight (Phytophthora infestans), fungal wilts (Fusarium sp. Verticillium sp. Rhizoctonia sp.) and bacterial wilt (Ralstonia solanacearum). Plant parasitic nematodes and pests (thrips, aphids, spider mites) were also reported in the study area. There was a significant(P<0.05) association between the following variables; type of land owner and major use of land, type of land owner and cropping system, source of agricultural information and whether or not to apply pesticides into the soil. Farmers were quite knowledgeable about tomato farming as they had access to information from various sources; however there are still major gaps in knowledge especially on diseases and pests. Keywords: Tomato, diseases, pests, nematodes, managemen

Differences In \u3ci\u3eUrochloa\u3c/i\u3e Hybrids and Cultivars Biomass Production in Several Sites in Western Kenya

Forage production is at the core of improved livestock productivity, especially in sub Saharan Africa. The genetic potential of existing animals remains underutilized due to limited forage quality and quantity. Albeit wide range of forage germplasm that exists, little data is available for identifying suitable genotypes, matched to specific environments and production systems. Due to the spatial and temporal diverse environments in which livestock production happens, multi-locational screening of forage production and characterizing genotype by environment interaction is key. We selected seven Urochloa (Syn. Brachiaria) genotypes comprising three hybrids and four cultivars and established them in on-farm trials in western Kenya for dry matter evaluation and nutritional quality. We selected eight sites covering four administrative counties (Siaya, Kakamega, Busia, Bungoma), and each county hosting two replicated trials, with each trial replicated 3 times. We observed dry matter yield differences across the counties in the order Bungoma \u3e Busia \u3e Kakamega \u3e Siaya. Similarly, the genotypes returned varied performance across the sites. Hybrids did well in one of the county, a mix of hybrids and cultivars in two counties and cultivars in the last county. Amongst sites, variation was least in Busia, and more pronounced in Bungoma. Continued assessments in subsequent cuts are underway. These will feed into context-specific recommendations about suitable forages for sustainable intensification in the face of global warming

Effect of Daily Antenatal Iron Supplementation on Plasmodium Infection in Kenyan Women: A Randomized Clinical Trial.

IMPORTANCE: Anemia affects most pregnant African women and is predominantly due to iron deficiency, but antenatal iron supplementation has uncertain health benefits and can increase the malaria burden. OBJECTIVE: To measure the effect of antenatal iron supplementation on maternal Plasmodium infection risk, maternal iron status, and neonatal outcomes. DESIGN, SETTING, AND PARTICIPANTS: Randomized placebo-controlled trial conducted October 2011 through April 2013 in a malaria endemic area among 470 rural Kenyan women aged 15 to 45 years with singleton pregnancies, gestational age of 13 to 23 weeks, and hemoglobin concentration of 9 g/dL or greater. All women received 5.7 mg iron/day through flour fortification during intervention, and usual intermittent preventive treatment against malaria was given. INTERVENTIONS: Supervised daily supplementation with 60 mg of elemental iron (as ferrous fumarate, n = 237 women) or placebo (n = 233) from randomization until 1 month postpartum. MAIN OUTCOMES AND MEASURES: Primary outcome was maternal Plasmodium infection at birth. Predefined secondary outcomes were birth weight and gestational age at delivery, intrauterine growth, and maternal and infant iron status at 1 month after birth. RESULTS: Among the 470 participating women, 40 women (22 iron, 18 placebo) were lost to follow-up or excluded at birth; 12 mothers were lost to follow-up postpartum (5 iron, 7 placebo). At baseline, 190 of 318 women (59.7%) were iron-deficient. In intention-to-treat analysis, comparison of women who received iron vs placebo, respectively, yielded the following results at birth: Plasmodium infection risk: 50.9% vs 52.1% (crude difference, -1.2%, 95% CI, -11.8% to 9.5%; P = .83); birth weight: 3202 g vs 3053 g (crude difference, 150 g, 95% CI, 56 to 244; P = .002); birth-weight-for-gestational-age z score: 0.52 vs 0.31 (crude difference, 0.21, 95% CI, -0.11 to 0.52; P = .20); and at 1 month after birth: maternal hemoglobin concentration: 12.89 g/dL vs 11.99 g/dL (crude difference, 0.90 g/dL, 95% CI, 0.61 to 1.19; P < .001); geometric mean maternal plasma ferritin concentration: 32.1 µg/L vs 14.4 µg/L (crude difference, 123.4%, 95% CI, 85.5% to 169.1%; P < .001); geometric mean neonatal plasma ferritin concentration: 163.0 µg/L vs 138.7 µg/L (crude difference, 17.5%, 95% CI, 2.4% to 34.8%; P = .02). Serious adverse events were reported for 9 and 12 women who received iron and placebo, respectively. There was no evidence that intervention effects on Plasmodium infection risk were modified by intermittent preventive treatment use. CONCLUSIONS AND RELEVANCE: Among rural Kenyan women with singleton pregnancies, administration of daily iron supplementation, compared with administration of placebo, resulted in no significant differences in overall maternal Plasmodium infection risk. Iron supplementation led to increased birth weight. TRIAL REGISTRATION: clinicaltrials.gov Identifier: NCT01308112

Comparison of demographic and clinical characteristics between pulmonary and extra-pulmonary tuberculosis patients in Kiambu County, 2012-2015

Background: Tuberculosis (TB) continues to be a public health challenge globally. The most common organ to be involved is the lung although it can affect any organ in the body. The diagnosis of extra-pulmonary TB (EPTB) has faced many challenges mainly due to inadequate expertise to diagnose or lack of equipment for diagnosis.Objective: To compare the demographic and clinical characteristics between pulmonary and extrapulmonary tuberculosis in Kiambu CountyDesign: Retrospective cross-sectional studySetting: Kiambu County, KenyaSubjects: Tuberculosis patients notified in TIBU surveillance systemResults: Of the 15, 833 patients analyzed, 2,704 (17%) had extra-pulmonary tuberculosis. Male to female ratio was 1:1.7 in PTB and 1:1.3 in EPTB patients. There was declining trend of TB cases notified over the years for both PTB and EPTB. Pleural TB accounted for 38% with TB lymphadenitis accounting for 14% of the EPTB subtypes. TB-HIV co-infection was higher among EPTB (36%) compared to PTB (30%). The treatment success rate was 85% and 86% among PTB and EPTB cases respectively. The mortality was 10% among EPTB and 5% in PTB cases. The 5-14 age category were more likely to developing EPTB compared to PTB (AOR 4.67 95% CI (1.5-13.99). Kabete zone was most affected with EPTB (AOR 2.11(1.19-2.74) while a protective factor was observed among the HIV positive clients (AOR 0.58 (0.43 - 0.78)Conclusion: There was a general decline in cases for both EPTB and PTB. However, the age category most affected was 5-14 years. The co-infectivity rate was higher among the EPTB patients compared to the PTB patients. High index of suspicion and appropriate diagnostic tools are needed in evaluation particularly in EPTB which will assist in early management of the patients. ART uptake could play a big role in protecting HIV positive clients from getting EPTB

Understanding the association between skin involvement and joint activity in patients with psoriatic arthritis: experience from the Corrona Registry.

Objective: To compare the characteristics of patients with psoriatic arthritis among patient groups stratified by degree of skin and joint involvement, and to evaluate the relationship between skin severity and joint activity. Methods: Body surface area (BSA) and Clinical Disease Activity Index (CDAI) at enrolment were analysed. Patient characteristics were stratified by skin severity and joint activity. Baseline patient characteristics, clinical and disease characteristics and patient-reported outcomes were compared. The strength of the relationship of skin severity and joint activity was evaluated using methods for categorical variables (χ Results: 1542 adult patients in the Corrona Psoriatic Arthritis/Spondyloarthritis Registry enrolled between 21 May 2013 and 20 September 2016 were analysed. Most patients in the BSA \u3e3%/CDAI moderate/high subgroup had worse clinical and patient-reported outcomes. A significant (p Conclusion: Skin severity is modestly correlated with joint activity, and patients with higher skin severity are two times more likely to have increased joint involvement. Clinicians need to address both skin severity and joint activity in treatment decisions

An assessment of the magnitudes and factors associated with postharvest losses in off-farm grain stores in Kenya

Off-farm grain storage is an important postharvest undertaking by government, traders and processors in Kenya. A survey was conducted in 2014/2015 to assess the kinds and magnitudes of perceived losses experienced in off-farm stores, and the factors associated with them. Store supervisors or key personnel charged with grain storage in 39 public and 74 private stores, spread across the six maize growing agro-ecological zones, were interviewed using a structured questionnaire. Total perceived losses averaged 17.6± 2.3%, and were attributed to insects (7.2± 1.0%), molds (5.7± 2.1%), moisture loss (3.4± 0.5%), rodents (2.0± 0.5%), spillage (0.50 ± 0.0%) and birds (0.10± 0.0%). Total losses experienced in public and privately owned stores were not significantly different. The losses attributed to insect and the vertebrate pests, moisture loss and spillage, were also not significantly different in the two storage systems. However, losses due to molds were significantly higher in the private stores. From regression analysis, higher losses were associated with the use of residual insecticides, purchasing low quality maize for storage, reuse of storage bags, untimely control of storage pests, and the agro-climatic conditions of the moist transitional, moist mid-altitude and dry mid-altitude zones. Relying on infestation reports to initiate pest control had the highest marginal effect (21.7%). Lower loss magnitudes were associated with cleaning and drying grain before storage, early pest control and storage periods shorter than two months. These findings provide important reasons for appraising current off-farm storage techniques with a view to taking possible actions for improvements. It is recommended that innovations to mitigate maize postharvest losses should also target off-farm storage, as the losses incurred are significant

Blockade of adenosine A2B receptors ameliorates murine colitis

Background and purpose: The adenosine 2B (A2B) receptor is the predominant adenosine receptor expressed in the colon. Acting through the A2B receptor, adenosine mediates chloride secretion, as well as fibronectin and interleukin (IL)-6 synthesis and secretion in intestinal epithelial cells. A2B receptor mRNA and protein expression are increased during human and murine colitis. However, the effect of the A2B receptor in the activation of the intestinal inflammatory response is not known. In this study, we examined the effect of A2B receptor antagonism on murine colitis. Experimental approach: Dextran sodium sulphate (DSS)-treated mice and piroxicam-treated IL-10/ mice were used as animal models of colitis. The A2B receptor-selective antagonist, ATL-801, was given in the diet. Key results: Mice fed ATL-801 along with DSS showed a significantly lower extent and severity of colitis than mice treated with DSS alone, as shown by reduced clinical symptoms, histological scores, IL-6 levels and proliferation indices. The administration of ATL-801 prevented weight loss, suppressed the inflammatory infiltrate into colonic mucosa and decreased epithelial hyperplasia in piroxicam-treated IL-10/ mice. IL-6 and keratinocyte-derived chemokine (KC) concentrations in the supernatants of colonic organ cultures from colitic mice were significantly reduced by ATL-801 administration. Conclusions and implications: Taken together, these data demonstrate that the intestinal epithelial A2B receptor is an important mediator of pro-inflammatory responses in the intestine and that A2B receptor blockade may be an effectiv

Intestinal parasitic infections in schoolchildren in different settings of Côte d'Ivoire : effect of diagnostic approach and implications for control

BACKGROUND: Social-ecological systems govern parasitic infections in humans. Within the frame of assessing the accuracy of a rapid diagnostic test for Schistosoma mansoni in Cote d'Ivoire, three different endemicity settings had to be identified and schoolchildren's intestinal parasitic infection profiles were characterized. METHODS: In September 2010, a rapid screening was conducted in 11 schools in the Azaguie district, south Cote d'Ivoire. In each school, 25 children were examined for S. mansoni and S. haematobium. Based on predefined schistosome endemicity levels, three settings were selected, where schoolchildren aged 8-12 years were asked to provide three stool and three urine samples for an in-depth appraisal of parasitic infections. Triplicate Kato-Katz thick smears were prepared from each stool sample for S. mansoni and soil-transmitted helminth diagnosis, whereas urine samples were subjected to a filtration method for S. haematobium diagnosis. Additionally, a formol-ether concentration method was employed on one stool sample for the diagnosis of helminths and intestinal protozoa. Multivariable logistic regression models were employed to analyse associations between schoolchildren's parasitic infections, age, sex and study setting. RESULTS: The prevalences of S. mansoni and S. haematobium infections in the initial screening ranged from nil to 88% and from nil to 56%, respectively. The rapid screening in the three selected areas revealed prevalences of S. mansoni of 16%, 33% and 78%. Based on a more rigorous diagnostic approach, the respective prevalences increased to 92%, 53% and 33%. S. haematobium prevalences were 0.8%, 4% and 65%. Prevalence and intensity of Schistosoma spp., soil-transmitted helminths and intestinal protozoan infections showed setting-specific patterns. Infections with two or more species concurrently were most common in the rural setting (84%), followed by the peri-urban (28.3%) and urban setting (18.2%). CONCLUSIONS: More sensitive diagnostic tools or rigorous sampling approaches are needed to select endemicity settings with high fidelity. The observed small-scale heterogeneity of helminths and intestinal protozoan infections has important implications for contro