Suicide in Kenyan Universities

This study examines the prevalence of suicidal thinking, planning, attempts and self-harm and the associated psychological stressors in two Kenyan universities. The participants are undergraduate university students aged (18 -29) years. A convergent mixed method study design was adopted to collect data. Self-administered questionnaire and focus group discussions were carried out among 431 students and six university counselors participated in a three rounds delphi survey. The study instruments were adopted from the Suicide Assessment Five-Step Evaluation & Triage (SAFE-T), Columbia Suicide Severity Rating Scale (C-SSRS), Non-Suicidal Self-Injury Assessment Tool (NSSI-AT) and Emerging Adult Stress Inventory (EASI). A multivariate analysis (MANOVA) was performed. The prevalence rate of suicidal thinking was 17.1%, suicidal planning 5.9%, suicidal attempts 7.8% and self-harm 5.5%. Female students and private university students had high prevalence for all suicidal behaviors. Feeling hopeless and depressed, financial difficult, feeling anxious, family conflicts and academic challenges were psychological stressors compelling emerging adults to suicidal behaviors. The prevalence for suicidal behaviors were similar to that found in other studies carried out in other countries. Suicidal behaviors are associated with mental illness symptoms. This should be taken into account by peers, parents and university counselors to prevent and intervene for suicidal behaviors

Suicidal Behaviors Among Emerging Adults in Kenyan Universities

This study examined the prevalence of suicidal thinking, planning, attempts, and self-harm and the associated psychological stressors in two Kenyan universities. The participants were undergraduate university students aged (18 -29) years. A convergent mixed-method study design was adopted to collect data. Self-administered questionnaires and focus group discussions were carried out among 431 students and six university counselors participated in a three-round Delphi survey. The study instruments were adopted from the Suicide Assessment Five-Step Evaluation & Triage (SAFE-T), Columbia Suicide Severity Rating Scale (C-SSRS), Non-Suicidal Self-Injury Assessment Tool (NSSI-AT), and Emerging Adult Stress Inventory (EASI). A multivariate analysis (MANOVA) was performed. The prevalence rate of suicidal thinking was 17.1%, suicidal planning 5.9%, suicidal attempts 7.8%, and self-harm 5.5%. Female students and private university students have a high prevalence of all suicidal behaviors. Feeling hopeless and depressed, financial difficulty, feeling anxious, family conflicts, and academic challenges were psychological stressors compelling emerging adults to suicidal behaviors. The prevalence of suicidal behaviors was like that found in other studies carried out in other countries. Suicidal behaviors are associated with mental illness symptoms. This should be considered by peers, parents, and university counselors to prevent and intervene in suicidal behaviors

Suicide in Kenyan Universities

This study examines the prevalence of suicidal thinking, planning, attempts and self-harm and the associated psychological stressors in two Kenyan universities. The participants are undergraduate university students aged (18 -29) years. A convergent mixed method study design was adopted to collect data. Self-administered questionnaire and focus group discussions were carried out among 431 students and six university counselors participated in a three rounds delphi survey. The study instruments were adopted from the Suicide Assessment Five-Step Evaluation & Triage (SAFE-T), Columbia Suicide Severity Rating Scale (C-SSRS), Non-Suicidal Self-Injury Assessment Tool (NSSI-AT) and Emerging Adult Stress Inventory (EASI). A multivariate analysis (MANOVA) was performed. The prevalence rate of suicidal thinking was 17.1%, suicidal planning 5.9%, suicidal attempts 7.8% and self-harm 5.5%. Female students and private university students had high prevalence for all suicidal behaviors. Feeling hopeless and depressed, financial difficult, feeling anxious, family conflicts and academic challenges were psychological stressors compelling emerging adults to suicidal behaviors. The prevalence for suicidal behaviors were similar to that found in other studies carried out in other countries. Suicidal behaviors are associated with mental illness symptoms. This should be taken into account by peers, parents and university counselors to prevent and intervene for suicidal behaviors

Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals

Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon-γ (IFN-γ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of β-D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P<0.05) increased IFN-γ mRNA expression by foal neutrophils on days 3, 9, and 14. Degranulation was significantly (P<0.05) lower for foals in the CpG-ODN-treated group than the control group at days 3 and 14, but not at other days. No effect of treatment on ROS generation was detected. These results indicate that CpG-ODN administration to foals might improve innate and adaptive immune responses that could protect foals against infectious diseases and possibly improve responses to vaccination.The open access fee for this work was funded through the Texas A&M University Open Access to Knowledge (OAK) Fund

Effect of the antimicrobial peptide, D-hecate, on Trichomonads

Tritrichomonas foetus and Trichomonas vaginalis are protozoan parasites that cause sexually transmitted diseases in cattle and humans, respectively. There is a need for new antimicrobial agents to treat or prevent trichomoniasis because there are currently no approved chemotherapeutic agents against T. foetus and resistance of T. vaginalis to metronidazole does occur. Therefore, we evaluated the effect of a novel antimicrobial peptide, D-hecate, on the viability of 6 isolates of T. foetus and T. vaginalis in vitro. Tritrichomonas foetus and T. vaginalis were grown to mid log phase (24 hr) or late log/stationary phase (48 hr). Parasites at 106/ml were mixed with equal volumes of D-hecate to final concentrations of 10 μM, 20 μM, and 40 μM of D-hecate. Controls had minimal essential medium (MEM) alone. The numbers of viable parasites were determined microscopically after 10, 20, and 30 min of incubation at 37 C with D-hecate or MEM. Our results show that D-hecate killed all 6 isolates of T. foetus and T. vaginalis evaluated. The killing effect was dependent on the concentration of the peptide, incubation time, and phase of growth of the parasites. Ultrastructural studies of parasites treated with 10 μM of D-hecate revealed extensive damage to the plasma membrane of most T. foetus and T. vaginalis cells, while a few cells were distorted but remained intact. D-Hecate may be a useful chemotherapeutic agent for the treatment of trichomoniasis

Demonstration of \u3ci\u3eTritrichomonas foetus\u3c/i\u3e in the External Genitalia and of Specific Antibodies in Preputial Secretions of Naturally Infected Bulls

Portions of penis and prepuce were collected from 24 bulls with current or recent Tritrichomonas foetus infection. Epididymides were collected from seven of the bulls, and seminal vesicles and prostate were collected from four. Following immunohistochemical staining with two monoclonal antibodies (34.7C4.4 and TF1.15) prepared against T. foetus surface antigens, trichomonads were identified in sections from 15 of the bulls. Organisms were most often located in penile crypts in the midshaft and caudal regions and less often in preputial crypts. Trichomonads were not observed in sections from other genitalia or in subepithelial tissue. T. foetus antigen, however, was present in the cytoplasm of some epithelial cells and the cytoplasm of some mononuclear cells in subepithelial lymphoid aggregates and follicles. Preputial smegma was collected from 16 T. foetus-infected bulls and from 16 control bulls with negative T. foetus cultures. Preputial antibody levels to TF1.17, a surface antigen of T. foetus, were determined by an enzyme-linked immunosorbent assay. Preputial secretions from infected bulls contained specific antibody of each isotype and subisotype tested. IgG1 responses were the greatest, IgM and IgA responses were approximately equal, and IgG2 responses were low. Each isotype and subisotype response in infected bulls was significantly greater than that in the controls. These results confirm previous speculation concerning anatomical sites of infection and suggest that parasite antigen can be taken up and processed locally, resulting in deposition of specific IgG1, IgG2, IgA, and IgM antibodies in the preputial cavity

The role of agricultural institutions in providing support towards sustainable rural development in South Pacific Island countries

This paper examines the agricultural training in higher education institutions and tertiary colleges, their pre-eminent role and how best they can contribute to alleviate poverty in rural communities in Fiji and other South Pacific island countries. These institutions provide support through training farmers (vocational and adult education) and/or extension officers and providing researchers. Unfortunately, agricultural training institutions are not adapting to the rapid changing times early enough and have more or less maintained the traditional way of training. There is a need for agricultural institutions to amend their programs to facilitate the new emerging areas, together with new learning and teaching frameworks, establish new partnerships with the private sector in addition to expanding their representation in governance in addition to holding continuous dialogue with policymakers. Further, these institutions can potentially showcase local customs and knowledge, mirroring the regional culture, and ethical customs of the Pacific island community, as well as global movements and development forces. In reinforcing their title role as contributors to a culture of education and rural agricultural development, we suggest that agricultural institutions engage more directly and more effectively in partnerships and dialogue with other local agricultural stakeholders and their surrounding rural communities in Fiji and other Pacific island countries

Antimicrobial resistance management in Pacific Island countries: Current status, challenges, and strategic solutions

Antimicrobial resistance (AMR) is currently recognized as a major emerging threat to human and animal health. The burden of antimicrobial-resistant infections affects the economy in developed and developing countries. There is a rapid rise in AMR in human and veterinary medicine globally. AMR profiles are poorly documented in Fiji, and limited data are accessible. Fiji currently has no national veterinary antibiotic resistance surveillance network or regulations and guidelines on veterinary drug use. However, available literature shows that although human drugs are better managed than veterinary drugs, the knowledge is still constrained and dispersed. Furthermore, Fiji was chosen as a case study to develop a prototype AMR surveillance and control in the Pacific region. Pacific Island countries share similar geographic and climatic conditions. Currently, the Australian Centre for International Agricultural Research has funded an AMR project that addresses some gaps in managing AMR in the region. The project is the first to adopt the One Health approach to research the AMR in humans, animals, and the Pacific region’s environment. Combating AMR needs human health and veterinary personnel to work with all other stakeholders. Continuous surveillance for resistant clinical isolates in humans and animals and the development of appropriate policy intervention measures in human and veterinary drug use are necessary to alleviate AMR burden. Therefore, there is a need to educate farmers, human patients, and the public on the fight against AMR. In addition, AMR data are necessary to develop effective AMR control strategies. This review gives a comprehensive information assessment on AMR in Fiji and the other South Pacific Islands in relation to global trends. Suggestions on the most appropriate ways of effectively managing AMR in Fiji have been mad