Kondisi Subpopulasi Limfosit Penderita Early-Onset Periodontitis Pengunjung Klinik Periodonsia Rumah Sakit Gigi & Mulut FKG UI

In the last decade, much attention has been devoted to study immunologic factors in early-onset periodontitis (EOP). This study was designed to investigate peripheral blood lymphocyte subpopulations cells in patients with EOP, 32 patients with EOP and 10 normal healthy control subjects were included in the study. Peripheral blood T-lymphocytes, helper T-cells, suppressor T-cells, B-lymphocytes, natural killer (NK) cells and the Th/Ts ratio were determined using appropriate monoclonal antibodies and the indirect immunofluorescence method. In the EOP group, T-lymphocytes and helper T-cells were found to be significantly lowere where as NK cells was significantly higher than the control group. They had normal numbers of B-lymphocytes, suppressor T-cells, and the Th/Ts ratio. These findings could contribute to the science about EOP in Indonesia, and to the immunopathogenesis of EOP

DISCOLORATION OF TOOTH ENAMEL DUE TO BETEL LEAF EXTRACT (Piper betle Linn): DISKOLORISASI EMAIL GIGI AKIBAT EKSTRAK DAUN SIRIH (Piper betle Linn)

The tooth enamel colour play an important role in esthetics. The discoloured teeth could be due to the using of boild Piper betle extract as a mouth wash. Up to now many people in Indonesia using the boiling Piper betle extract to manage oral diseases and to prevent tooth decay. The aim of this in vitro study are to know the effective concentration and application time of this agent to change the enamel colour. Fresh Piper betle leaves were processed to get 4 differents concentration (50, 33, 25, and 5%). Aquabidest was used as negative control. Every concentration was studyed using 2 maxillae premolar teeth in 24, 48, and 72 hours application times. 30 good condition teeth were used in this research.    The results showed that every concentration gaves discoloured changes. The optimal concentration and duration are 25% and 48 hours. It is concluded that using of this gargling agent must be intermittent. It can be suggested to use this agent only once or twice daily, each for 2 until 3 minutes gargling

ACTIVITY OF PERICARP EXTRACT OF MANGOSTEEN AGAINST ORAL STREPTOCOCCI: AKTIVITAS EKSTRAK PERICARP BUAH MANGGIS MELAWAN STREPTOCOCCI ORAL

The increasing prevalence of dental caries is still as a major world health problem. Caries is the direct result of acid production by cariogenic oral pathogens, especially Streptococcus mutans. New and better antimicrobial agents active against cariogenic bacteria with minimal side effects on the oral tissues are much needed, especially natural agents derived directly from plants. Phytochemical studies have shown that the extracts from various parts of mangosteen or Garciniamangostana Linn tree contain varieties of secondary metabolites such as prenylated and oxygenated xanthones, many of which have been found in vitro to have antimicrobial properties against oral pathogens. Several studies which examined the eficacy of herbal for human health have shown that xanthones from mangosteen have remarkable biological activities such as antioxidant, antimicrobial, anticancer etc, and had no cytotoxic effects on human gingival fibroblasts. Their results showed that among these xanthone derivatives obtain from pericarp extract of mangosteen, α-mangostin has the most potent antimicrobial activity against cariogenic Streptococcus mutans. It can be concluded that the strong antimicrobial activity of the pericarp extract of mangosteen is a good drug of choice that might be helpful in preventing the dental caries

DETEKSI DNA BABI PADA PRODUK SOSIS MENGGUNAKAN REAL TIME–POLYMERASE CHAIN REACTION (RT–PCR)

Sosis adalah salah satu produk olahan daging yang saat ini di Indonesia sudah diperjualbelikan sampai ke pinggiran kota bahkan ke desa-desa. Bahan baku sosis adalah daging sapi, ayam, ikan dan babi. Sampai saat ini harga daging sapi dirasakan oleh banyak konsumen masih sangat mahal. Namun, ternyata produsen sosis banyak yang menjual produknya dengan harga yang sangat murah. Kondisi tersebut dapat menimbulkan kecurigaan dari konsumen jangan-jangan ada penggantian atau pencampuran daging sapi dengan atau oleh daging babi. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah untuk menguji kandungan daging babi (DNA babi) pada produk sosis yang dijual di pasar-pasar tradisional di Jakarta dan di gerai-gerai frozen food di perkampungan sekitar Jakarta. Pengujian DNA babi menggunakan metode Real Time Polymerace Chain Reaction (RT-PCR). Hasil penelitian menunjukkan bahwa semua sampel uji (10 sampel uji) dengan merek yang berbeda baik yang mencantumkan atau tidak mencantumkan logo halal MUI, semuanya tidak terdeteksi mengandung DNA babi

EFFECTIVENESS OF LAWSONIA INERMIS LINNEAUS LEAVES INFUSION IN GINGIVITIS HEALING: EFEKTIFITAS INFUSA DAUN LAWSONIA INERMIS LINNEAUS TERHADAP PENYEMBUHAN GINGIVITIS

Gingivitis is one type of periodontal disease and it is chronic in nature. The main etiological factor of gingivitis is bacterialplaque. Untreated gingivitis can spread to underlying tissues and it can become periodontitis and produce tissuedestruction. One of the treatments for gingivitis is plaque removal, helped by antibacterial mouthrinsing to reducebacterial plaque. Lawsonia inermis L. leaves have been used by Indonesian villagers for healing skin wounds andreported to show antibacterial effect in vitro and in vivo. This study aimed to determine the effect of Lawsonia inermis L.leaves infusion in gingivitis healing. A total of 63 gingivitis patients devided into 5 groups were instructed to rinse with 3concentrations (50000 µg/mL, 10000 µg/mL, 5000 µg/mL) of Lawsonia inermis L. leaves infusion; 0.1% hexetidinesolution, and placebo as control. Papilla Bleeding Index (PBI) used in measurement for bleeding on probing. The resultshowed that decreasing of PBI in Lawsonia inermis L. leaves infusion at 10000 µg/mL concentratation (80%), wasstronger than hexetidine 0.1% (76%). In conclusion, Lawsonia inermis L. leaves infusion effective to reduce PBI betterthan hexetidine. In conclusion, Lawsonia inermis L. leaves is effective to treat gingivitis

BAKTERI PATOGEN PERIODONTAL PUTATIF PADA IBU HAMIL SEBAGAI PENYEBAB KELAHIRAN BAYI PREMATUR DENGAN BERAT BADAN LAHIR RENDAH

Preterm low birth weight (PLBW) infants are more likely to die during the neonatal period, and PLBW survivors face neurodevelopmental problems, respiratory problems, and congenital anomalies. It is said that the poor periodontal health of the pregnant woman is a potential risk factor of PLBW. This investigation aims to study the oral hygiene and gingival inflammation condition of 2nd and 3rd trimester of pregnancy women and relate to the putative periodontal pathogen bacteriae as the probable cause of PLBW. The plaque index (PII), calculus index (CI), and bleeding on probing (BOP) of the subjects of both groups were measured and compared. Maternal infection during pregnancy has been demonstrated to perturb cytokines and hormone-regulated gestation because of the translocation of the infection. The level of gingival crevicular fluid prostaglandin E2 (PG E2), interleukin 1β (IL-1β), IL-6, tumor necrotizing factors α (TNF-α) are usually highly correlated with those in amnion fluid of pregnant women without bacterial vaginosis (BV) and with intact placental membrane. It is concluded that the periodontal health of pregnant women must be in good condition to prevent the risk of having PLBW still need to be anticipated due to the presence of the inflammation signs

PENGARUH PASTA GIGI YANG MENGANDUNG ENZIM TEHADAP PENURUNAN KADAR VOLATILE SULFUR COMPOUND : EFFECT OF ENZYME TOOTH PASTE IN DECREASING VOLATILE SULFUR COMPOUND LEVEL

Halitosis or breath malodor may have negative efffect in communication. Halitosis is caused by chemical sulfur known as volatile sulfur compound (VSC) as a result of sulfur containing of amino acid degradation by anaerobic bacteria. Halitosis can be prevented by toothbrushing with tooth paste. The aim of this study was to analyze the effect of teeth and tongue brushing with dentrifice containing of amiloglucooxidase and glucooxidase enzyme. This experimental clinic study was conducted in the Clinic of Periodontology, Faculty of Dentistry, University of Indonesia. Subjects consisted of 77 students aged 17-30 years old, divided into 2 groups, 39 subjects conducted to brush their teeth and tongue with amiloglucooxidase and glucooxidase enzyme and 38 subjects conducted to brush their teeth and tongue with non enzyme tooth paste. The measurement of VSC level used cromatography gas, conducted after 30 minutes after teeth and tongue brushing. The result showed that there was no statical different in reducing VSC components in both groups (p>0,05). In conclusion, teeth and tongue brushing with dentrifice containing amiloglucooxidase and glucooxidase enzyme were not different to decrease the VSC level compared with non enzyme paste

Analisis Antibodi IgG Spesifik Fimbria Porphyromonas gingivalis dengan Metoda Elisa pada Penderita Early-Onset Periodontitis

The adherence of bacteria to the host tissues is a first step in the development of infections. Bacterial fimbriae have been shown to play an important role in the interaction between bacteria and host cells or among bacterial cells. Optical density of serum IgG specific Porphyromonas gingivalis strain ATCC 33277 fimbriae antibodies has been estimated in 32 cases of Early-Onset Periodontitis and 14 healthy periodontal subjects as control. The mean level of IgG were significantly elevated (p=0,000) in their cases. The data support the role of P. gingivalis as a key pathogen in Early-Onset Periodontitis

In-vitro studies on antioxidant and antidiabetic potential of Sesoot (Garcinia picrorrhiza Miq.) fruit ethanolic extract from Indonesia

Diabetes mellitus (DM) is a disease that can be identified by high levels of blood glucose. Garcinia plants have been widely used for many traditional medicines as antioxidant, anticancer, antidiabetics, and antiinflammation. The antioxidant and antidiabetic activities of (Garcinia picrorrhiza Miq.) or sesoot fruit extract were evaluated in this study and compared with xanthone. The antioxidant and antidiabetic of ethanolic ripe sesoot (G. picrorrhiza Miq.) fruit extract (GpKar) was evaluated by (ABTS) reducing activity, α-glucosidase, β-glucosidase, and α-amylase inhibitor activity. GpKar showed higher ABTS-reducing activity (IC50 = 49.30 µg/mL) than xanthone (IC50 = 404.30 µg/mL). GpKar showed IC50 = 109.32 µg/mL for α-glucosidase inhibitory activity, while xanthones had a better activity (IC50 = 33.97 µg/mL). GpKar also showed lower α-amylase inhibitory activity and  β-glucosidase (IC50 = 126.01 and 9432.09 µg/mL) compared to xanthone (IC50 = 44.32 and 405.03 µg/mL, respectively). The compounds of GpKar are proven to have antioxidant and antidiabetic activities. Therefore, it will be industrially relevant to develop a natural medicine for decreasing DM risk, thus evaluating the antioxidant and antidiabetic effect of G. picrorrhiza by a pre-clinic study is needed

In-vitro studies on antioxidant and antidiabetic potential of Sesoot (Garcinia picrorrhiza Miq.) fruit ethanolic extract from Indonesia

Diabetes mellitus (DM) is a disease that can be identified by high levels of blood glucose. Garcinia plants have been widely used for many traditional medicines as antioxidant, anticancer, antidiabetics, and antiinflammation. The antioxidant and antidiabetic activities of (Garcinia picrorrhiza Miq.) or sesoot fruit extract were evaluated in this study and compared with xanthone. The antioxidant and antidiabetic of ethanolic ripe sesoot (G. picrorrhiza Miq.) fruit extract (GpKar) was evaluated by (ABTS) reducing activity, α-glucosidase, β-glucosidase, and α-amylase inhibitor activity. GpKar showed higher ABTS-reducing activity (IC50 = 49.30 µg/mL) than xanthone (IC50 = 404.30 µg/mL). GpKar showed IC50 = 109.32 µg/mL for α-glucosidase inhibitory activity, while xanthones had a better activity (IC50 = 33.97 µg/mL). GpKar also showed lower α-amylase inhibitory activity and β-glucosidase (IC50 = 126.01 and 9432.09 µg/mL) compared to xanthone (IC50 = 44.32 and 405.03 µg/mL, respectively). The compounds of GpKar are proven to have antioxidant and antidiabetic activities. Therefore, it will be industrially relevant to develop a natural medicine for decreasing DM risk, thus evaluating the antioxidant and antidiabetic effect of G. picrorrhiza by a pre-clinic study is needed