Interstitial laser therapy of benign prostatic hyperplasia

The objective of interstitial laser coagulation (ILC) of benign prostatic enlargement is to achieve a marked volume reduction and to decrease bladder outlet obstruction and lower urinary tract symptoms with minimal morbidity. Coagulation necrosis is generated well inside the adenoma by means of specifically designed laser applicators combined with either a Nd:YAG laser or a diode laser. Because the laser applicators can be inserted as deeply and as often as necessary, it is possible to coagulate any amount of tissue at any desired location while preserving adjacent structures such as the urethra. Postprocedural, the intraprostatic lesions result in secondary atrophy and regression of the prostate lobes, rather than sloughing of necrotic tissue. Several single-armed and randomized studies indicated the effectiveness of interstitial laser coagulation of BPH-syndrome. Marked improvements in AUA score, peak flow rate, residual urine volume and prostate volume were reported. Pressure-flow studies demonstrated a sufficient decrease of the intravesical/detrusor pressure, urethral opening pressure and urethral resistance. Long-term results demonstrating sustained success for up to 3 years were reported on a series of 394 patients. ILC is suitable to debulk even large prostates and to treat highly obstructive patients. Therefore, ILC can be seen as a true alternative to TURF with certain advantages, such as almost no serious morbidity and with certain disadvantages, such as the need for postoperative catheterization. However, ILC can be done under local anesthesia and does not require hospitalization

IZBOR DOBAVLJAČA I VIŠEKRITERIJALNA ANALIZA : Diplomski rad

U ovom je radu prikazan kvantitativni model izbora dobavljača. Model koristi tri metode operacijske pretrage: hijerarhijski proces analize, PROMETHEE metodu i linearno programiranje. Prva metoda, AHP, koristi se za izračun težina svakog kriterija pomoću usporedbe u parovima. Koristeći PROMETHEE metodu alternative (dobavljači) rangiraju se te se izračunavaju koeficijenti preferencije za svakog dobavljača. LP model, koji se temelji na prethodno prikupljenim koeficijentima, distribuira količine narudžbi svakom dobavljaču u svrhu maksimiziranja TVP-a.This thesis presents a supplier selection quantitative model. The model deals with three methods of operation research: Analytic Hierarchy Process, PROMETHEE method and linear programming. The first of them – AHP – is used to calculate the weights of each criteria by pairwise comprehension. By using the PROMETHEE method the alternatives (suppliers) are ranked and preference coefitions for each supplier are calculated. The LP model, which is based on the previous gathered coefitions, distributes order quantities to each supplier in order to maximise the Total Value of Purchasing (TVP)

Understanding Person Identification Through Gait

Gait recognition is the process of identifying humans from their bipedal locomotion such as walking or running. As such, gait data is privacy sensitive information and should be anonymized where possible. With the rise of higher quality gait recording techniques, such as depth cameras or motion capture suits, an increasing amount of detailed gait data is captured and processed. Introduction and rise of the Metaverse is but one popular application scenario in which the gait of users is transferred onto digital avatars. As a first step towards developing effective anonymization techniques for high-quality gait data, we study different aspects of movement data to quantify their contribution to gait recognition. We first extract categories of features from the literature on human gait perception and then design experiments for each category to assess how much the information they contain contributes to recognition success. Our results show that gait anonymization will be challenging, as the data is highly redundant and interdependent

Temporal Integration Windows in Neural Processing and Perception Aligned to Saccadic Eye Movements

SummaryWhen processing dynamic input, the brain balances the opposing needs of temporal integration and sensitivity to change. We hypothesized that the visual system might resolve this challenge by aligning integration windows to the onset of newly arriving sensory samples. In a series of experiments, human participants observed the same sequence of two displays separated by a brief blank delay when performing either an integration or segregation task. First, using magneto-encephalography (MEG), we found a shift in the stimulus-evoked time courses by a 150-ms time window between task signals. After stimulus onset, multivariate pattern analysis (MVPA) decoding of task in occipital-parietal sources remained above chance for almost 1 s, and the task-decoding pattern interacted with task outcome. In the pre-stimulus period, the oscillatory phase in the theta frequency band was informative about both task processing and behavioral outcome for each task separately, suggesting that the post-stimulus effects were caused by a theta-band phase shift. Second, when aligning stimulus presentation to the onset of eye fixations, there was a similar phase shift in behavioral performance according to task demands. In both MEG and behavioral measures, task processing was optimal first for segregation and then integration, with opposite phase in the theta frequency range (3–5 Hz). The best fit to neurophysiological and behavioral data was given by a dampened 3-Hz oscillation from stimulus or eye fixation onset. The alignment of temporal integration windows to input changes found here may serve to actively organize the temporal processing of continuous sensory input

Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading

Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA) pathogenesis, but there is no information on reliable housekeeping genes available. Therefore the goal of this study was to identify a set of reliable housekeeping genes suitable for studies of mechanical loading on SF from healthy and OA patients. Nine genes were evaluated towards expression stability and ranked according their relative stability determined by four different mathematical procedures (geNorm, NormFinder, BestKeeper and comparative ΔCq). We observed that RPLP0 (ribosomal protein, large, P0) and EEF1A1 (eukaryotic translation elongation factor 1 alpha 1) turned out to be the genes with the most stable expression in SF from non-OA or OA patients treated with or without mechanical loading. According to geNorm two genes are sufficient for normalization throughout. Expression of one tested target gene varied considerably, if normalized to different candidate housekeeping genes. Our study provides a tool for accurate and valid housekeeping gene selection in gene expression experiments on SF from healthy and OA patients with and without mechanical loading in consistent with the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines and additionally demonstrates the impact of proper housekeeping gene selection on the expression of the gene of interest

Reliable and redundant FPGA based read-out design in the ATLAS TileCal Demonstrator

The Tile Calorimeter at ATLAS is a hadron calorimeter based on steel plates and scintillating tiles read out by PMTs. The current read-out system uses standard ADCs and custom ASICs to digitize and temporarily store the data on the detector. However, only a subset of the data is actually read out to the counting room. The on-detector electronics will be replaced around 2023. To achieve the required reliability the upgraded system will be highly redundant. Here the ASICs will be replaced with Kintex-7 FPGAs from Xilinx. This, in addition to the use of multiple 10 Gbps optical read-out links, will allow a full read-out of all detector data. Due to the higher radiation levels expected when the beam luminosity is increased, opportunities for repairs will be less frequent. The circuitry and firmware must therefore be designed for sufficiently high reliability using redundancy and radiation tolerant components. Within a year, a hybrid demonstrator including the new read-out system will be installed in one slice of the ATLAS Tile Calorimeter. This will allow the proposed upgrade to be thoroughly evaluated well before the planned 2023 deployment in all slices, especially with regard to long term reliability. Different firmware strategies alongside with their integration in the demonstrator are presented in the context of high reliability protection against hardware malfunction and radiation induced errors

Towards a VR Evaluation Suite for Tactile Displays in Telerobotic Space Missions

Research and development of telerobotic systems supplemented by haptic feedback for future planetary exploration missions has gained significant importance in the past decade. Major space agencies endeavor to deploy such systems before sending humans to the surface of unknown or unexplored celestial bodies. Astronauts control these telerobotic systems from remote locations, such as an orbital space station. Haptic feedback for teleoperating the robots in outer space is extremely important, not only to improve user immersion and task performance, but also to improve our understanding of surface properties. At the same time, for spaceflight, making use of compact, light-weight and robust devices are preferred for precise tactile feedback from telemanipulation tasks. In this paper, we introduce "ViESTac", a first attempt to develop a generic VR suite to be able to evaluate and compare fingertip-wearable tactile devices. Applications of such a suite include, but are not limited to allowing teleoperators to judiciously choose suitable tactile devices for a particular task. To account for the wide variety of existing fingertip-wearable tactile devices and their display capabilities, the suite contains a set of virtual scenarios to investigate different tactile properties of virtual objects. It also dedicates a virtual scenario to evaluate how tactile feedback may govern the accuracy of human positioning in standard tasks. This proposed suite is advocated by a pilot study with 13 participants and two distinct state-of-the-art tactile devices. Results of the study clearly indicate that the virtual suite can successfully cater to the need of evaluating and comparing fingertip-wearable tactile devices

Impact of Mechanical Load on the Expression Profile of Synovial Fibroblasts from Patients with and without Osteoarthritis

Osteoarthritis (OA) affects the integrity of the entire joint including the synovium. The most abundant cells in the synovium are fibroblasts (SF). Excessive mechanical loading might contribute to OA pathogenesis. Here, we investigate the effects of mechanical loading on SF derived from non-OA (N-SF) and OA patients (OA-SF). We treated N-SF and OA-SF with or without mechanical loading for 48h after 24h of preincubation. Then we assessed gene and protein expression of proinflammatory factors (TNF, COX-2, PG-E2, IL-6), extracellular matrix (ECM) components (COL1, FN1) and glycosaminoglycans (GAGs) via RT-qPCR, ELISA, DMMB assay and HPLC. Mechanical loading significantly increased TNF and PG-E2 secretion by N-SF and OA-SF, whereas in OA-SF IL-6 secretion was reduced. COL1 and FN1 secretion were downregulated in N-SF during loading. OA-SF secreted less COL1 compared to N-SF under control conditions. In contrast, OA-SF in general expressed more FN1. GAG synthesis was upregulated in N-SF, but not in OA-SF during loading with OA-SF displaying a higher charge density than N-SF. Mechanical loading enhanced proinflammatory factor expression and GAG synthesis and decreased secretion of ECM components in N-SFs, indicating a contributing role of SF to OA development

Mechanical stress induce PG-E2 in murine synovial fibroblasts originating from the temporomandibular joint

Genetic predisposition, traumatic events, or excessive mechanical exposure provoke arthritic changes in the temporomandibular joint (TMJ). We analysed the impact of mechanical stress that might be involved in the development and progression of TMJ osteoarthritis (OA) on murine synovial fibroblasts (SFs) of temporomandibular origin. SFs were subjected to different protocols of mechanical stress, either to a high-frequency tensile strain for 4 h or to a tensile strain of varying magnitude for 48 h. The TMJ OA induction was evaluated based on the gene and protein secretion of inflammatory factors (Icam-1, Cxcl-1, Cxcl-2, Il-1ß, Il-1ra, Il-6, Ptgs-2, PG-E2), subchondral bone remodelling (Rankl, Opg), and extracellular matrix components (Col1a2, Has-1, collagen and hyaluronic acid deposition) using RT-qPCR, ELISA, and HPLC. A short high-frequency tensile strain had only minor effects on inflammatory factors and no effects on the subchondral bone remodelling induction or matrix constituent production. A prolonged tensile strain of moderate and advanced magnitude increased the expression of inflammatory factors. An advanced tensile strain enhanced the Ptgs-2 and PG-E2 expression, while the expression of further inflammatory factors were decreased. The tensile strain protocols had no effects on the RANKL/OPG expression, while the advanced tensile strain significantly reduced the deposition of matrix constituent contents of collagen and hyaluronic acid. The data indicates that the application of prolonged advanced mechanical stress on SFs promote PG-E2 protein secretion, while the deposition of extracellular matrix components is decreased

β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis

Purpose: Recent studies demonstrated a contribution of adrenoceptors (ARs) to osteoarthritis (OA) pathogenesis. Several AR subtypes are expressed in joint tissues and the β2-AR subtype seems to play a major role during OA progression. However, the importance of β2-AR has not yet been investigated in knee OA. Therefore, we examined the development of knee OA in β2-AR-deficient (Adrb2-/- ) mice after surgical OA induction. Methods: OA was induced by destabilization of the medial meniscus (DMM) in male wildtype (WT) and Adrb2-/- mice. Cartilage degeneration and synovial inflammation were evaluated by histological scoring. Subchondral bone remodeling was analyzed using micro-CT. Osteoblast (alkaline phosphatase - ALP) and osteoclast (cathepsin K - CatK) activity were analyzed by immunostainings. To evaluate β2-AR deficiency-associated effects, body weight, sympathetic tone (splenic norepinephrine (NE) via HPLC) and serum leptin levels (ELISA) were determined. Expression of the second major AR, the α2-AR, was analyzed in joint tissues by immunostaining. Results: WT and Adrb2-/- DMM mice developed comparable changes in cartilage degeneration and synovial inflammation. Adrb2-/- DMM mice displayed elevated calcified cartilage and subchondral bone plate thickness as well as increased epiphyseal BV/TV compared to WTs, while there were no significant differences in Sham animals. In the subchondral bone of Adrb2-/- mice, osteoblasts activity increased and osteoclast activity deceased. Adrb2-/- mice had significantly higher body weight and fat mass compared to WT mice. Serum leptin levels increased in Adrb2-/- DMM compared to WT DMM without any difference between the respective Shams. There was no difference in the development of meniscal ossicles and osteophytes or in the subarticular trabecular microstructure between Adrb2-/- and WT DMM as well as Adrb2-/- and WT Sham mice. Number of α2-AR-positive cells was lower in Adrb2-/- than in WT mice in all analyzed tissues and decreased in both Adrb2-/- and WT over time. Conclusion: We propose that the increased bone mass in Adrb2-/- DMM mice was not only due to β2-AR deficiency but to a synergistic effect of OA and elevated leptin concentrations. Taken together, β2-AR plays a major role in OA-related subchondral bone remodeling and is thus an attractive target for the exploration of novel therapeutic avenues