Calibración de sensores de humedad capacitivos usando redes neuronales

X Jornadas de Investigación de la Zona no Saturada del Suelo, Salamanca (España), 2011El estudio de la variabilidad espacial de la humedad del suelo a escala de parcela o cuenca agrícola requiere el uso de redes de sensores de humedad de bajo coste, que suelen mostrar una fiabilidad limitada y requieren de calibraciones específicas, especialmente en suelos con elevados contenidos en arcilla. El presente trabajo pretende plantear una calibración más fiable de sondas de humedad mediante un análisis mixto campo-laboratorio. Para la calibración de campo se dispone de datos gravimétricos; para la calibración en laboratorio se han usado columnas de suelo inalterado que tras ser saturadas fueron desecadas en un entorno controlado mientras se monitorizaba la evolución de su peso y la de su humedad volumétrica, medida con diferentes sondas capacitivas Decagon. Tras obtener curvas de secado y la relación entre la humedad gravimétrica y la volumétrica es posible realizar una calibración mejorada específica para cada tipo de suelo. Las redes neuronales son particularmente útiles para el modelado de procesos físicos y el ajuste de modelos. En este trabajo se propone el empleo de dichas herramientas para obtener calibraciones para las sondas analizadas en el tipo de suelo objeto de estudio. Los resultados muestran que dichas calibraciones permiten mejorar la precisión de las mediciones de humedad realizadas.The study of the spatial variability of soil water content at agricultural plot or catchment scales requires the use of low-cost soil water content sensor networks, which usually show a limited reliability and require specific calibrations, specially for soils with a high clay content. This work proposes a more reliable calibration of soil water content probes with a laboratory analysis. Minimally disturbed soil columns were saturated with water and dried in a controlled environment while monitorizing the evolution of their volumetric soil water content (with different capacitive Decagon Probes) and weights. After obtaining the drying curves and the relation between the volumetric and the measured gravimetric soil water contents it is possible to achieve an improved calibration specific for different kinds of soil. Neural networks are especially interesting for the modeling of physical processes and model adjustment. In this work, these tools were used in order to obtain improved calibrations for the analyzed probes in the studied soil type. Results show that this calibration improves the accuracy and pMinisterio de Ciencia e Innovación AGL2009 C03-03Junta de Andalucía AGR-478

Probing pre-formed alpha particles in the ground state of nuclei

In this Letter, we report on alpha particle emission through the nuclear break-up in the reaction 40Ca on a 40Ca target at 50A MeV. It is observed that, similarly to nucleons, alpha particles can be emitted to the continuum with very specific angular distribution during the reaction. The alpha particle properties can be understood as resulting from an alpha cluster in the daughter nucleus that is perturbed by the short range nuclear attraction of the collision partner and emitted. A time-dependent theory that describe the alpha particle wave-function evolution is able to reproduce qualitatively the observed angular distribution. This mechanism offers new possibilities to study alpha particle properties in the nuclear medium.Comment: 4 pages, 3 figure

Assessment of a New ROS1 Immunohistochemistry Clone (SP384) for the Identification of ROS1 Rearrangements in Patients with Non–Small Cell Lung Carcinoma: the ROSING Study

Introduction: The ROS1 gene rearrangement has become an important biomarker in NSCLC. The College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology testing guidelines support the use of ROS1 immunohistochemistry (IHC) as a screening test, followed by confirmation with fluorescence in situ hybridization (FISH) or a molecular test in all positive results. We have evaluated a novel anti-ROS1 IHC antibody (SP384) in a large multicenter series to obtain real-world data. Methods: A total of 43 ROS1 FISH-positive and 193 ROS1 FISH-negative NSCLC samples were studied. All specimens were screened by using two antibodies (clone D4D6 from Cell Signaling Technology and clone SP384 from Ventana Medical Systems), and the different interpretation criteria were compared with break-apart FISH (Vysis). FISH-positive samples were also analyzed with next-generation sequencing (Oncomine Dx Target Test Panel, Thermo Fisher Scientific). Results: An H-score of 150 or higher or the presence of at least 70% of tumor cells with an intensity of staining of 2+ or higher by the SP384 clone was the optimal cutoff value (both with 93% sensitivity and 100% specificity). The D4D6 clone showed similar results, with an H-score of at least 100 (91% sensitivity and 100% specificity). ROS1 expression in normal lung was more frequent with use of the SP384 clone (p < 0.0001). The ezrin gene (EZR)-ROS1 variant was associated with membranous staining and an isolated green signal FISH pattern (p = 0.001 and p = 0.017, respectively). Conclusions: The new SP384 ROS1 IHC clone showed excellent sensitivity without compromising specificity, so it is another excellent analytical option for the proposed testing algorithm

Evolution of the use of corticosteroids for the treatment of hospitalised COVID-19 patients in Spain between March and November 2020: SEMI-COVID national registry

Objectives: Since the results of the RECOVERY trial, WHO recommendations about the use of corticosteroids (CTs) in COVID-19 have changed. The aim of the study is to analyse the evolutive use of CTs in Spain during the pandemic to assess the potential influence of new recommendations. Material and methods: A retrospective, descriptive, and observational study was conducted on adults hospitalised due to COVID-19 in Spain who were included in the SEMI-COVID- 19 Registry from March to November 2020. Results: CTs were used in 6053 (36.21%) of the included patients. The patients were older (mean (SD)) (69.6 (14.6) vs. 66.0 (16.8) years; p < 0.001), with hypertension (57.0% vs. 47.7%; p < 0.001), obesity (26.4% vs. 19.3%; p < 0.0001), and multimorbidity prevalence (20.6% vs. 16.1%; p < 0.001). These patients had higher values (mean (95% CI)) of C-reactive protein (CRP) (86 (32.7-160) vs. 49.3 (16-109) mg/dL; p < 0.001), ferritin (791 (393-1534) vs. 470 (236- 996) µg/dL; p < 0.001), D dimer (750 (430-1400) vs. 617 (345-1180) µg/dL; p < 0.001), and lower Sp02/Fi02 (266 (91.1) vs. 301 (101); p < 0.001). Since June 2020, there was an increment in the use of CTs (March vs. September; p < 0.001). Overall, 20% did not receive steroids, and 40% received less than 200 mg accumulated prednisone equivalent dose (APED). Severe patients are treated with higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%. Conclusions: Patients with greater comorbidity, severity, and inflammatory markers were those treated with CTs. In severe patients, there is a trend towards the use of higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk

Genome-Wide Association Study in BRCA1 Mutation Carriers Identifies Novel Loci Associated with Breast and Ovarian Cancer Risk

BRCA1-associated breast and ovarian cancer risks can be modified by common genetic variants. To identify further cancer risk-modifying loci, we performed a multi-stage GWAS of 11,705 BRCA1 carriers (of whom 5,920 were diagnosed with breast and 1,839 were diagnosed with ovarian cancer), with a further replication in an additional sample of 2,646 BRCA1 carriers. We identified a novel breast cancer risk modifier locus at 1q32 for BRCA1 carriers (rs2290854, P = 2.7×10-8, HR = 1.14, 95% CI: 1.09-1.20). In addition, we identified two novel ovarian cancer risk modifier loci: 17q21.31 (rs17631303, P = 1.4×10-8, HR = 1.27, 95% CI: 1.17-1.38) and 4q32.3 (rs4691139, P = 3.4×10-8, HR = 1.20, 95% CI: 1.17-1.38). The 4q32.3 locus was not associated with ovarian cancer risk in the general population or BRCA2 carriers, suggesting a BRCA1-specific associat

A case-only study to identify genetic modifiers of breast cancer risk for BRCA1/BRCA2 mutation carriers

Breast cancer (BC) risk for BRCA1 and BRCA2 mutation carriers varies by genetic and familial factors. About 50 common variants have been shown to modify BC risk for mutation carriers. All but three, were identified in general population studies. Other mutation carrier-specific susceptibility variants may exist but studies of mutation carriers have so far been underpowered. We conduct a novel case-only genome-wide association study comparing genotype frequencies between 60,212 general population BC cases and 13,007 cases with BRCA1 or BRCA2 mutations. We identify robust novel associations for 2 variants with BC for BRCA1 and 3 for BRCA2 mutation carriers, P < 10−8, at 5 loci, which are not associated with risk in the general population. They include rs60882887 at 11p11.2 where MADD, SP11 and EIF1, genes previously implicated in BC biology, are predicted as potential targets. These findings will contribute towards customising BC polygenic risk scores for BRCA1 and BRCA2 mutation carriers

Thrombosis and agennesis of the rami spinales of the avertebralis in bullfighting cattle and the claudicatio intermittents during the fight

We have carried out macro and microscopical studies of the spinal rami of the a. vertebralis in seven backbones of fight heifers from the Pablo Romero Livestock, wich collapsed 2 or3 times during the filth-t. We wished to verify if the intermittent collapse is correlated with a medular ischemia, and if this is due to a thrombosis of the mentioned arteries. In the study carried out only a thrombus was found (table I), but we also found agennesis of the spinal arteries in 41 of the 98 dissected vessels. To verify if this agennesis was the cause for the intermittent collapse, we examined two hfifers wich did no collapse during the fight, and we found the same extent of agennesis of spinal rami in these test animals. Therefore we can deduce that the agennesis is not a sufficient factor for the intermittent trouble and that thrombosis is a rather rare condition.Hemos realizado un estudio macro y microscópico de los ramos espi¬nales de las arterias vertebrales pertenecientes a siete raquis de otras tantas becerras de la ganadería de Pablo Romero, las cuales se habían caldo durante la tienta, para comprobar si la claudicación intermitente del ganado vacuno de lidia corresponde a una isquemia medular, y si ésta se debe a la presencia de procesos trombóticos en dichas arterias. En las disecciones realizadas hallamos sólo un trombo organizado; en cambio comprobamos la ausencia de ramos espinales de la a. vertebrales en 41 de los 98 agujeros de conjunción disecados. Para comprobar si esta agenesia era determinante de la claudicación intermitente estudiamos dos testigos no caldos y encontramos que endichos testigos también exis¬tía el mismo grado de agenesia arterial Así, pues, deducimos que dichas agenesia§ no son factores suficientes de la claudicación intermitente y que la presencia de trombos en dichas arterias es rara