Comparative study of various intra ocular lens formulae by IOL master

Background: The aim and objective of the study was to calculate intraocular lens power with IOL master in 100 eyes of 100 patients with long axial lengths between 25mm to 32mm.To analyse and compare the results of various formulae by postoperative auto refractometry and corrected distance visual acuity and to know the most accurate formula for highly myopic eyes (Axial length more than 25.00 mm).Methods: Patients coming to Sarojini Devi Eye Hospital from December 2012-September 2014 for cataract surgery were considered in this study. All patients with visually significant cataract having fundus findings within normal limits were included in this study and patients of complicated cataract due to trauma, uveitis, Glaucoma and any corneal pathology were excluded from the study.Results: The Mean AL was 27.25 ± 1.25 mm, the Mean keratometric value was 43.62 ± 1.45 D, and the Mean Absolute Error (MAE) calculated by the Haigis was 0.07 DD. Compared to the MAEs generated by the other formulae, the MAE generated by the Haigis was comparable to that by the SRK/T (0.231 D), and significantly lower than those by the Hoffer Q (0.481 D) and Holladay (0.864 D).Conclusions: The Mean post-operative refractive error (spherical equivalent) was found to be the least with Haigis formula followed by SRK/T for eyes with long axial length.The HAIGIS formula has a better predictability and accuracy. The postoperative hyperopic shift was comparable between HAIGIS and SRK-T formulae the least postoperative hyperopic shift with Haigis formula compared to other formulae.

In silico approach towards the identification of potential inhibitors from Curcuma amada Roxb against H. pylori: ADMET screening and molecular docking studies

Introduction: The present study attempts to identify potential targets of H. pylori for novel inhibitors from therapeutic herb, mango ginger (Curcuma amada Roxb.). Methods: Crystal structure of all the selected drug targets obtained from Protein Data Bank (PDB) were subjected to molecular docking against a total of 130 compounds (found to have biological activity against H. pylori) were retrieved from public databases. Compounds with good binding affinity were selected for Prime MM-GBSA rescoring and molecular dynamics (MD) simulation. Final list of compounds were taken for ADMET predictions. Results: Based on binding affinity denoted by glide score and ligand efficiency, mango ginger compounds were found selective to shikimate kinase and type II dehydroquinase through hydrogen bonding and salt bridge interactions. Stability of the interactions and free energy calculations by Prime MM-GBSA results confirmed the affinity of mango ginger compounds towards both shikimate kinase and type II dehydroquinase. From the above results, 15 compounds were calculated for ADMET parameters, Lipinski’s rule of five, and the results were found promising without any limitations. MD simulations identified gentisic acid as hit compound for shikimate kinase of H. pylori. Conclusion: Current study could identify the in silico potential of mango ginger compounds against shikimate kinase and type II dehydroquinase targets for H. pylori infections and are suitable for in vitro and in vivo evaluation