Intoxication with some pesticides induce release of cytochrome C and DNA fragmentation in human cell line Huh 7

USMF „N. Testemiţanu” Catedra Biochimie şi Biochimie Clinică; Universitatea din Bucureşti, Facultatea de Biologie, Catedra BiochimieLindane and deltametrine are insecticides with very large utilization in agriculture and public health wich are dangerous contaminants for human organism. We studied the capacity of lindane and deltametrin to induce release of cytochrome C and DNA fragmentation in human cell line Huh 7, as a posibele mecanism of toxiticity. Our results sugest that intoxication with lindane and deltametrin induce biochemical modifications related to apoptosis. Lindanul şi deltametrinul sunt insecticide pe larg utilizate în agricultură şi sănătate publică, periculoase ca contaminanţi pentru organismul uman. Noi am studiat capacitatea acestor insecticide de a provoca eliberarea citocromului c şi fragmentatrea ADN în culturi de hepatocite umane transformate Huh7, ca un posibil mecanism al toxicităţii lor urmat de declansarea prcesului apoptotic. Rezultatele obţinute, sugerează că intoxicaţia cu aceste pesticide induce modificările biochimice caracteristice apoptozei

The Carina dSph galaxy: where is the edge?

Recent cosmological N-body simulations suggest that current empirical estimates of tidal radii in dSphs might be underestimated by at least one order of magnitude. To constrain the plausibility of this theoretical framework, we undertook a multiband (U,B,V,I) survey of the Carina dSph. Deep B,V data of several fields located at radial distances from the Carina center ranging from 0.5 to 4.5 degrees show a sizable sample of faint blue objects with the same magnitudes and colors of old, Turn-Off stars detected across the center. We found that the (U-V,B-I) color-color plane is a robust diagnostic to split stars from background galaxies. Unfortunately, current U,I-band data are too shallow to firmly constrain the real extent of Carina.Comment: To be published on the proceedings of the XLIX meeting of the Italian Astronomical Society. Requires mem.cl

Control and Supervision of Wind Energy Conversion Systems

This paper is about a PhD thesis and includes the study and analysis of the performance of an onshore wind energy conversion system. First, mathematical models of a variable speed wind turbine with pitch control are studied, followed by the study of different controller types such as integer-order controllers, fractional-order controllers, fuzzy logic controllers, adaptive controllers and predictive controllers and the study of a supervisor based on finite state machines is also studied. The controllers are included in the lower level of a hierarchical structure composed by two levels whose objective is to control the electric output power around the rated power. The supervisor included at the higher level is based on finite state machines whose objective is to analyze the operational states according to the wind speed. The studied mathematical models are integrated into computer simulations for the wind energy conversion system and the obtained numerical results allow for the performance assessment of the system connected to the electric grid. The wind energy conversion system is composed by a variable speed wind turbine, a mechanical transmission system described by a two mass drive train, a gearbox, a doubly fed induction generator rotor and by a two level converter

Electrochromic and electrofluorochromic properties of a new boron dipyrromethene–ferrocene conjugate

A new boron dipyrromethene–ferrocene (BODIPY–Fc) conjugate with pentafluorophenyl as the meso substituent and two Fc termini was synthesized and its spectroscopic and electrochemical features were analyzed. An intramolecular charge transfer from the donor Fc to the acceptor BODIPY has been predicted by theory and confirmed experimentally, leading to efficient fluorescence quenching when the dyad is in the neutral state. Fluorescence can be triggered by oxidizing both ferrocenyl units either chemically or electrochemically. Eventually, a fully reversible fluorescence switch is evidenced by coupling TIRF microscopy with electrolysis in an electrochemical cell

Classic vs laparoscopic approach in colorectal cancer. Experience of a tertiary center, Surgery No 3 Clinic, Cluj-Napoca

Clinica Chirurgie 3, Cluj-Napoca, România, Al XIII-lea Congres al Asociației Chirurgilor „Nicolae Anestiadi” și al III-lea Congres al Societății de Endoscopie, Chirurgie miniminvazivă și Ultrasonografie ”V.M.Guțu” din Republica MoldovaIntroducere: Cancerul colorectal este unul dintre cele mai frecvente cancere și cu tendință în creștere la nivel global. Majoritatea studiilor recente au demonstrat non inferioritatea și chiar o ușoară superioritate în abordul laparoscopic prin prisma rezultatelor obținute și a supraviețuirii la distanță. Material și metode: Au fost selectate retrospectiv 2186 de cazuri din baza de date completată prospectiv a Clinicii Chirurgie 3 pentru perioada ian 2013-dec 2018 (6 ani). Din acestea s-au exclus 76 cazuri laparoscopie/laparotomie exploratorie, 154 cazuri colostomii, 51 derivații interne; în final au fost analizate 1905 cazuri de cancer colorectal. Rezultate: Din 1905 cazuri s-au efectuat rezecții laparoscopice la un număr de 310 (16.27%) și clasice la un număr de 1595 cazuri (83.73%). Au fost analizați între cele două loturi următorii parametri: pregătire preoperatorie, durata operației, pierderi sangvine, complicații postoperatorii (fistulă, abces, hemoragie, ocluzie, complicații generale), supurații de plagă, zile spitalizare, necesar antibiotic, mobilizare postoperatorie, mortalitate. Concuzii: Abordul laparoscopic prezintă avantaje privind recuperarea postoperatorie, pierderi sangvine, zile spitalizare, necesar antialgice/antibiotic, lipsa supurațiilor de plagă. Dezavantajele sunt curba de învățare, aparatura specifică și dificultatea păstrării principiilor oncologice.Introduction: Colorectal cancer remains one of the most frequently diagnosed malignant pathologies with a continuously increasing rate worldwide. Most of the recent studies have shown the non-inferiority and slight superiority in the laparoscopic approach through obtained results. Material and methods: 2186 cases were selected retrospectively from a prospectively completed database of the Surgical no 3 Clinic in Cluj-Napoca over the course of 6 years (ian 2013 – dec 2018). Out of these cases, 76 cases were excluded for exploratory laparoscopy/laparotomy, 154 which underwent only colostomy, and 51 which underwent internal derivation. At the end of the study, 1905 cases were eligible. Results: Out of 1905 cases, 310 underwent a laparoscopic approach (16.27%) and 1595 cases underwent a classic approach (83.73%). Between the two approaches, a series of parameters were analyzed: preoperative care, duration of the surgery, intraoperative blood loss, postoperative complications (fistula, abscess, hemorrhage, occlusion, general complications), antibiotic necessity, postoperative mobilization, mortality, prevalence of surgical site infection. Conclusions: The laparoscopic approach proves many advantages regarding postoperative care, blood loss, hospitalization care, necessity of antibiotics and painkillers, and surgical site infection, cosmetic advantages. Disadvantages are the learning curve, specific instruments requirements, difficulty of maintaining the oncology principles

Ti-Zr-Si-Nb nanocrystalline alloys and metallic glasses: Assessment on the structure, thermal stability, corrosion and mechanical properties

The development of novel Ti-based amorphous or \u3b2-phase nanostructured metallic materials could have significant benefits for implant applications, due to improved corrosion and mechanical characteristics (lower Young's modulus, better wear performance, improved fracture toughness) in comparison to the standardized \u3b1+\u3b2 titanium alloys. Moreover, the devitrification phenomenon, occurring during heating, could contribute to lower input power during additive manufacturing technologies. Ti-based alloy ribbons were obtained by melt-spinning, considering the ultra-fast cooling rates this method can provide. The titanium alloys contain in various proportions Zr, Nb, and Si (Ti60Zr10Si15Nb15, Ti64Zr10Si15Nb11, Ti56Zr10Si15Nb19) in various proportions. These elements were chosen due to their reported biological safety, as in the case of Zr and Nb, and the metallic glass-forming ability and biocompatibility of Si. The morphology and chemical composition were analyzed by scanning electron microscopy and energy-dispersive X-ray spectroscopy, while the structural features (crystallinity, phase attribution after devitrification (after heat treatment)) were assessed by X-ray diffraction. Some of the mechanical properties (hardness, Young's modulus) were assessed by instrumented indentation. The thermal stability and crystallization temperatures were measured by differential thermal analysis. High-intensity exothermal peaks were observed during heating of melt-spun ribbons. The corrosion behavior was assessed by electrocorrosion tests. The results show the potential of these alloys to be used as materials for biomedical applications

Rezultate preliminarii privind germinaţia şi velocitatea seminţelor de mazăre la soiul kelvedon wonder

The seed is an important factor of production that ensures the biological material necessary for the establishment of agricultural and horticultural crops. The seed of a cultivation will faithfully pass the characteristics determined by the genome if it has a higher cultural value, a primordial value determined by germination, purity and state of health. In the case of seed produced in the year 2016, the germination index varied from 84.0% determined to five days, up to 93.5% (on the ninth day). Germination speed is maximum at first determination (21.0%) and decreases to 8.5% when seed germination has reached the highest value during the 12 days. The velocity coefficient of germination has descended values, the lowest value of 8% registering on day 12, which can be genetically influenced, but also by storage conditions

Resection margin in liver metastasectomy secondary colorectal cancer

Clinica Chirurgie 3, Cluj-Napoca, România, Al XIII-lea Congres al Asociației Chirurgilor „Nicolae Anestiadi” și al III-lea Congres al Societății de Endoscopie, Chirurgie miniminvazivă și Ultrasonografie ”V.M.Guțu” din Republica MoldovaIntroducere: Rezectia hepatică este gold standardul în tratamentul metastazelor hepatice. Marginea de rezectie hepatica în cazul metastazelor hepatice a fost si este un subiect des dezbătut. Unii chirurgi considerau marginea de siguranță oncologică de 1 cm, în timp ce în ultima vreme se acceptă tot mai des marginea de rezecție de 1 mm. Materiale și metode: În studiul prezent au fost analizați 140 de pacienți internați din 01.01.2011 pana in 30.06.2018 si s-au comparat rezultatele obținute în funcție de marginile de rezecție R0 si R1. Rezultate și concluzii: Concluziile acestui studiu sunt că lipsa tratamentului neoadjuvant se corelează cu apariția mai frecventă marginii de rezecție microscopic pozitive, nu sunt diferențe semnificativ statistice de supraviețuire între pacienții cu R0 și R1, supraviețuirea acestora la 5 ani fiind de 45% vs. 33%, pe termen scurt și mediu, supraviețuirea estimată este mai mică în grupul pacienților cu metastaze mai mari de 5 cm, metastazele din hemificatul stang prezic un prognostic mai lung al supraviețuirii, iar supraviețuirea este influențată de gradul de diferențiere tumoral - biologia tumorală.Introduction: Liver resection is the gold standard in the treatment of liver metastases. The hepatic resection margin for liver metastases has been and is a frequently debated topic. Some surgeons considered the 1 cm margin of oncology safety, whereas lately, the 1 mm resection margin is increasingly accepted. Material and methods: In the current study, 140 patients were admitted from January 01, 2011 to June 30, 2018 and the results obtained according to the resection edges R0 and R1 were compared. Results and conclusion: The conclusion of this study is that the lack of neoadjuvant treatment correlates with the more frequent occurrence of the positive microscopic resection margin, there are no statistically significant survival differences between patients with R0 and R1, their survival at 5 years being 45% vs. 33%, in the short and medium term, the estimated survival is lower in the patients with metastases greater than 5 cm, the left hemi-liver metastases predict a longer prognosis of survival, and survival is influenced by the degree of tumor differentiation - tumor biology

On the k-Symplectic, k-Cosymplectic and Multisymplectic Formalisms of Classical Field Theories

The objective of this work is twofold: First, we analyze the relation between the k-cosymplectic and the k-symplectic Hamiltonian and Lagrangian formalisms in classical field theories. In particular, we prove the equivalence between k-symplectic field theories and the so-called autonomous k-cosymplectic field theories, extending in this way the description of the symplectic formalism of autonomous systems as a particular case of the cosymplectic formalism in non-autonomous mechanics. Furthermore, we clarify some aspects of the geometric character of the solutions to the Hamilton-de Donder-Weyl and the Euler-Lagrange equations in these formalisms. Second, we study the equivalence between k-cosymplectic and a particular kind of multisymplectic Hamiltonian and Lagrangian field theories (those where the configuration bundle of the theory is trivial).Comment: 25 page

Сравнительная оценка структуры заболеваемости студентов I курса Государственного Медицинского и Фармацевтического Университета им. Н. A. Тестемицану

Department of Family Medicine and Laboratory of Genetics, Nicolae Testemiţanu State Medical and Pharmaceutical University, Chisinau, Republic of Moldova, Congresul III al Medicilor de Familie din Republica Moldova, 17–18 mai, 2012, Chişinău, Republica Moldova, Conferinţa Naţională „Maladii bronhoobstructive la copii”, consacrată profesorului universitar, doctor habilitat Victor Gheţeul, 27 aprilie, Chişinău, Republica MoldovaThe purpose of this work was to comparatively evaluate the morbidity spectrum of the first year students of the SMPhU ”Nicolae Testemiţanu” enrolled in 2002-2004 and 2010-2011. Materials and methods: the data of medical examination performed at the University Clinic of Primary Care were analyzed in order to estimate the morbidity spectra. Results: the comparative analysis of the health status of students revealed an increase of students being “sick” from 24.0% in 2002-2004 to 35.0% in 2010-2011. The comparative analysis of morbidity spectra denotes a significant increase in proportions of eye pathology, pathologies of urinary and digestive tracts and endocrine system in the morbidity structure during 2002-2011 period. Conclusions: our data support the need of medical examination of first-year students in order to identify the existing chronic pathologies, proper treatment, and monitoring their diseases during the academic years.Целью настоящей работы была сравнительная оценка структуры заболеваемости студентов первого курса Государственного Медицинского и Фармацевтического Университета имени „Николая Тестемицану”, поступивших на учебу в 2002-2004 и 2010-2011 годы. Материал и методы: результаты медицинского осмотра студентов первого курса 2002-2004 и 2010-2011 годов поступления, для определения спектра заболеваемости. Результаты: сравнительный анализ состояния здоровья студентов показал увеличение группы студентов «больных» от 24,0% в 2002-2004 гг. до 35,0% в 2010-2011 гг. В структуре заболеваемости за период 2002-2011 годы произошло значительное увеличение доли глазной патологии, патологии мочеполового, пищеварительного трактов и эндокринной системы. Выводы: Для выявления существующих хронических заболеваний, своевременного их лечения и мониторинга динамики состояния здоровья студентов во время учебы, необходимо проведение медицинского осмотра студентов на первом курсе