Editorial: Emerging Infectious and Vector-Borne Diseases: A Global Challenge

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Caractérisation d'un nouveau densovirus infectant le puceron Myzus persicae

National audienc

Characterization of a new densovirus infecting the green peach aphid Myzus persicae.

International audienceA new icosahedral DNA virus was isolated from aphids (Myzus persicae) that showed abnormal growth and development. The purified virus particles have a diameter of 20 nm and contain a single-stranded DNA molecule of approximately 5.7 kb. The viral particles are composed of five structural proteins (92, 85, 68, 64, and 57 kDa). As the main biophysical properties of this virus are similar to those of the members of the genus Densovirus it was tentatively named Myzus persicae densovirus (MpDNV). A PCR-based detection method and a polyclonal antiserum raised against MpDNV allowed the detection of the virus in a single-infected aphid. MpDNV is immunologically related to Junonia coenia densovirus, but not to other members of the subfamily Densovirinae. Biological assays showed that MpDNV could be both transmitted transovarially and horizontally via honeydew and saliva. MpDNV was able to infect whiteflies but not other aphid species tested

Subtype richness and sampling effort varies among studies.

<p>(a) Predicted AIV subtype richness (diamonds) with 95% confidence intervals based on the mean Chao2 richness estimator for seven distinct study locations for which the criteria of five sampling events and >5,000 birds tested non-discriminately for AIV subtypes were met (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone-0090826-t001" target="_blank">Table? 1</a>). Further, predicted AIV subtype Chao2 richness estimates are displayed from GenBank. (b) The sample size at each study location (blue bar) and minimum sufficient sampling size necessary to capture 75% of total estimated AIV richness (black dot). EGY  =  Egypt <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone.0090826-Soliman1" target="_blank">[50]</a>, PRT  =  Portugal <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone.0090826-Henriques1" target="_blank">[51]</a>, MNG  =  Mongolia (this study), EU  =  European Union (this study), CAN  =  Alberta, Canada <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone.0090826-Hinshaw1" target="_blank">[34]</a>, SWE  =  Sweden <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone.0090826-LatorreMargalef1" target="_blank">[14]</a>, TWN  =  Taiwan <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090826#pone.0090826-Cheng1" target="_blank">[37]</a>, and GB  =  sequences with subtypes deposited in GenBank (1959–2012).</p