Geographic Patterns of seed trait variation in an invasive species: how much can close populations differ?

Seeds play a major role in plant species persistence and expansion, and therefore they are essential when modeling species dynamics. However, homogeneity in seed traits is generally assumed, underestimating intraspecifc trait variability across the geographic space, which might bias species success models. The aim of this study was to evaluate the existence and consequences of interpopulation variability in seed traits of the invasive species Carpobrotus edulis at diferent geographical scales. We measured seed production, morphology, vigour and longevity of nine populations of C. edulis along the Catalan coast (NE Spain) from three diferentiated zones with a human presence gradient. Geographic distances between populations were contrasted against individual and multivariate trait distances to explore trait variation along the territory, evaluating the role of bioclimatic variables and human density of the diferent zones. The analysis revealed high interpopulation variability that was not explained by geographic distance, as regardless of the little distance between some populations (<0.5 km), signifcant diferences were found in several seed traits. Seed production, germination, and persistence traits showed the strongest spatial variability up to 6000% of percent trait variability between populations, leading to diferentiated C. edulis soil seed bank dynamics at small distances, which may demand diferentiated strategies for a cost-efective species management. Seed trait variability was infuenced by human density but also bioclimatic conditions, suggesting a potential impact of increased anthropogenic pressure and climate shifts. Geographic interpopulation trait variation should be included in ecological models and will be important for assessing species responses to environmental heterogeneity and change

A rapid and sensitive method to assess seed longevity through accelerated aging in an invasive plant species

Background: Seed longevity and vigor assessment is crucial for efficient ex situ biodiversity conservation in genebanks but may also have potential applications for the understanding of ecological processes and in situ biodiversity conservation. In fact, one of the factors determining the persistence of invasive species, a main threat to global biodiversity, is the generation of soil seed banks where seeds may remain viable for several years. Artificial seed aging tests using high temperatures and high relative humidity have been described for seed longevity estimation but have been mainly optimized for species with commercial interest. Thus, the aim of the study is to define a rapid and sensitive method to assess seed longevity and vigor through accelerated aging in the worldwide distributed invasive species Carpobrotus edulis to provide tools to biodiversity managers to evaluate invasive potential and develop effective post-eradication plans. Results:Slow seed deterioration rate was obtained when C. edulis seeds were subjected to common accelerated aging temperatures (43-45 °C). This contrasts with the rapid viability decay between 24-72 h when seeds were subjected to temperatures superior to 55 °C, a strong inflection point for this species' thermosensitivity. Relative humidity also played a role in defining seed survival curves, but only at high temperatures, speeding up the deterioration process. The selected aging conditions, 55 °C at 87% relative humidity were tested over two C. edulis populations and three measures were proposed to parametrize the differential sigmoidal seed survival curves, defining the seed resistance to deterioration (L5, aging time where 95% of seeds maintain their viability), medium longevity (L50, 50% of seeds lose their viability) and lethal aging time (L95, 95% of viability loss). Conclusions: An accelerated aging test at 55 °C and 87% relative humidity constitutes a rapid and sensitive method that can be performed within a working week, allowing managers to easily test seed vigor and longevity. This test may contribute to assess invasive potential, design effective monitoring programs and soil seed bank eradication treatments

Valoració per carpetes de les estades clíniques dels estudis de fisioteràpia de l'EUCS Manresa

L'objectiu general que ens proposem aconseguir al llarg d'aquesta assignatura, és integrar el procés d'avaluació dins del procés d'ensenyament-aprenentatge, i aconseguir que els mateixos estudiants esdevinguin més autònoms a l'hora de controlar el seu procés d'aprenentatge

Clinical Validation of the Vitro HPV Screening Assay for Its Use in Primary Cervical Cancer Screening

The advances in cervical cancer screening have mostly focused on high-risk human papillomavirus (HR HPV) detection as the primary screening tool in women over 30 years old. Although many new HPV assays have been commercialized during the last few years, only a few of them are validated according to international guidelines. Recently, new approaches for genotype-based risk-stratification and triage of HPV positive women have been developed. In this study, the Vitro HPV Screening assay targeting 14 oncogenic genotypes (HPV16, HPV18, and another 12 HR HPV genotypes) has demonstrated an appropriate clinical performance to be used as primary screening test. Additionally, the 12 HR HPV genotypes have been individually genotyped with a complementary assay performed by reverse dot blot hybridization of the PCR product from the HPV Screening assay. In conclusion, this n ew assay represents a unique integrated solution for cervical cancer screening with the extended HPV genotyping as a clinical tool for risk determination. Many scientific societies have issued guidelines to introduce population-based cervical cancer screening with HPV testing. The Vitro HPV Screening assay is a fully automatic multiplex real-time PCR test targeting the L1 GP5+/GP6+ region of HPV genome. The assay detects 14 high risk (HR) HPV genotypes, identifying individual HPV16 and HPV18 genotypes, and the HPV-positive samples for the other 12 HR HPV types are subsequently genotyped with the HPV Direct Flow Chip test. Following international guidelines, the aim of this study was to validate the clinical accuracy of the Vitro HPV Screening test on ThinPrep-collected samples for its use as primary cervical cancer screening, using as comparator the validated cobas (R) 4800 HPV test. The non-inferiority analysis showed that the clinical sensitivity and specificity of the Vitro HPV Screening assay for a diagnosis of cervical intraepithelial neoplasia of grade 2 or worse (CIN2+) were not inferior to those of cobas (R) 4800 HPV (p = 0.0049 and p < 0.001 respectively). The assay has demonstrated a high intra- and inter-laboratory reproducibility, also among the individual genotypes. The Vitro HPV Screening assay is valid for cervical cancer screening and it provides genotyping information on HPV-positive samples without further sample processing in a fully automated workflow

Charming and bewitching : considering the Harry Potter series

Aquest document està relacionat amb: Addictive and wonderful : the experience of reading the Harry Potter serie

Addictive and wonderful : the experience of reading the Harry Potter series

Aquest document està relacionat amb: Charming and bewitching : considering the Harry Potter serie

Processing overwhelms biostimulant‐induced vitamin E improvement in lentils

Abstract Although biostimulants are used in crop cultivation to improve productivity and/or quality of fruit and vegetables, their effect is rarely investigated up to processed food. Here, we investigated whether vitamin E content in lentils can be influenced by caffeine used as a biostimulant in a greenhouse‐controlled experiment, and how cooking influences its intake in our diet. Results showed that contents of γ‐tocopherol, the most abundant vitamin E homologue, increased by 27% upon biostimulant application during cultivation in raw lentils. However, these positive effects were completely abolished in the processed food, so cultivation differences had no effect on the cooked lentils. Cooking reduced by 43% and 50% of the contents of α‐ and γ‐tocopherols, respectively. A comparative study of vitamin E contents revealed that our lentil variety (variety ‘Pardina’ from ecological origin) contained more vitamin E than any other variety described thus far, with α‐ and γ‐tocopherol contents of 0.86 and 6.83 mg/100 g FW in raw lentils, and 0.49 and 3.42 mg/100 g FW in cooked lentils, respectively. A bowl of cooked ‘Pardina’ lentils, which are very typical in Mediterranean and Middle Eastern diets, can therefore represent a substantial proportion of the daily intake of vitamin E

Functional interplay between protein kinase CK2 and salicylic acid sustains PIN transcriptional expression and root development

13 Pags.- 2 Tabls.- 6 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12481/abstractWe have previously reported that CK2-defective Arabidopsis thaliana plants (CK2mut plants) were impaired severely in root development and auxin polar transport, and exhibited transcriptional misregulation of auxin-efflux transporters (Plant J., 67, 2011a, 169). In this work we show that CK2mut roots accumulate high levels of salicylic acid (SA) and that the gene that encodes isochorismate synthase (SID2) is overexpressed, strongly suggesting that CK2 activity is required for SA biosynthesis via the shikimate pathway. Moreover, SA activates transcription of CK2-encoding genes and, thus, SA and CK2 appear to be part of an autoregulatory feed-back loop to fine-tune each other's activities. We also show that exogenous SA and constitutive high SA levels in cpr mutants reproduce the CK2mut root phenotypes (decrease of root length and of number of lateral roots), whereas inhibition of CK2 activity in SA-defective and SA-signalling mutants lead to less severe phenotypes, suggesting that the CK2mut root phenotypes are SA-mediated effects. Moreover, exogenous SA mediates transcriptional repression of most of PIN-FORMED (PIN) genes, which is the opposite effect observed in CK2mut roots. These results prompted us to propose a model in which CK2 acts as a link between SA homeostasis and transcriptional regulation of auxin-efflux transporters. We also show that CK2 overexpression in Arabidopsis has neither impact on SA biosynthesis nor on auxin transport, but it improves the Arabidopsis root system. Thus, unlike the outcome in mammals, an excess of CK2 in plant cells does not produce neoplasia, but it might be advantageous for plant fitness. © 2014 John Wiley & Sons Ltd.This work was supported by grants BFU2010-15090 (Ministerio de Educación y Ciencia, Spain) and 2009SGR-795 (Generalitat de Catalunya, Catalunya, Spain). L.A. was recipient of a fellowship from the Ministerio de Educación y Ciencia (Spain). The authors declare to have no conflict of interest.Peer Reviewe

Valoració per carpetes de les estades clíniques dels estudis de fisioteràpia de l'EUCS Manresa

L'objectiu general que ens proposem aconseguir al llarg d'aquesta assignatura, és integrar el procés d'avaluació dins del procés d'ensenyament-aprenentatge, i aconseguir que els mateixos estudiants esdevinguin més autònoms a l'hora de controlar el seu procés d'aprenentatge

Application of a rapid and sensitive method for hormonal and Vitamin E profiling reveals crucial regulatory mechanisms in flower senescence and fruit ripening

Knowledge of ripeness and regulation of postharvest processes is an important tool to prevent loss of commercial value in both fruit and cut flower markets. The joint analysis of hormones and vitamin E levels can reveal complex interactions between hormones and oxidative stress as key regulators of postharvest processes. Profiling of both groups of metabolic compounds was performed during the ripening of non-climacteric fruits (red raspberry, Rubus idaeus L.) and senescence of ethylene-insensitive flowers (Dutch Iris, Iris x hollandica L.). After an initial extraction of the sample, without further purification steps, the hormonal profile was analyzed by UPLC-MS/MS and vitamin E levels were measured by HPLC. This methodological approach was very fast and had enough sensitivity for the analysis of small samples. Raspberry fruit maturation was characterized by a decline of cytokinin levels [zeatin, zeatin riboside, 2-isopentenyl adenine, and isopentenyl adenosine (Z, ZR, 2-iP, and IPA, respectively)] and gibberellins (GA1 in particular). Exogenous application of ABA prevented δ-tocopherol loss during fruit ripening. Iris floral senescence was also under strict hormonal control, also mediated by cytokinins and gibberellins. Z, ZR, 2-iP, GA9, and GA24 levels decreased in inner tepals, whereas the level of IPA decreased in style-merged-to-stigma tissues, thus suggesting tissue-specific roles for different hormones. α-Tocopherol levels decreased during senescence of inner tepals, hence suggesting enhanced oxidative stress. In conclusion, the rapid and sensitive hormonal and vitamin E profiling presented here can help in understanding the key physiological processes underlying fruit ripening and floral senescence