Feed the Future IPM Innovation Lab: A Critical Role in Global Food Security

The World Food Summit of 1966 defined ”food security” as existing “when all people at all times have access to sufficient, safe, and nutritious food to maintain a healthy and active life.” Food insecurity is part of a continuum that includes hunger (food deprivation), malnutrition (deficiencies, imbalances, or excess of nutrients), and famine. The world faces three major challenges: (1) to match the rapidly changing demand for food, (2) to do so in ways that are environmentally and socially acceptable, and (3) to ensure that the world’s poorest people are no longer hungry. World population is expected to reach 9 billion in 2050. To feed this population, there must be a 60–70% increase in food production. The effects of climate change must also be dealt with. The area under cultivation is not expected to expand to meet the gap, and we have yet to meet it by increasing yield per unit area and reducing losses in field and post-harvest handling. A concerted effort to reduce losses without jeopardizing environmental and public health concerns by adopting Integrated Pest Management (IPM) could reduce the loss by 50%, leading to a needed increase in food production of only 30%. Over several decades, the IPM Collaborative Research Program (CRSP) consortium developed IPM packages for tomatoes, other tropical vegetables, fruit, and grain crops and disseminated in host countries through research and extension arms. In addition, several national, regional, and international workshops have been conducted. The IPM Innovation Lab (new name for CRSP in 2013) is playing a vital role in the struggle for global food security. This will continue through the new Feed the Future IPM Innovation Lab which has expanded beyond a limited number of vegetables to include more vegetables, rice, fruit, maize, chickpea, climate change, and invasive species

Field performance of the parasitoid wasp, Trichogrammatoidea armigera (Hymenoptera: Trichogrammatidae) following releases against the millet head miner, Heliocheilus albipunctella (Lepidoptera: Noctuidae) in the Sahel

The effectiveness of the egg parasitoid Trichogrammatoidea armigera Nagaraja (Hymenoptera: Trichogrammatidae) in controlling Heliocheilus albipunctella de Joannis (Lepidoptera: Noctuidae), a major insect pest of pearl millet in the Sahel was assessed during two consecutive years in Niger on-station and on-farm conditions. We found that released T. armigera were able to find and parasitize host eggs within pearl millet fields both onstation and in farmers’ fields. On-station releases of T. armigera led to an average 4.86-fold increase in T. armigera parasitism compared to control fields, where no parasitoids were released. Likewise, on-farm releases of T. armigera led to up to 5.31-fold more egg parasitism by T. armigera in release fields than in control. Our results suggest the effectiveness of T. armigera and lays the groundwork for using T.armigera in augmentative biological control of H. albipunctella in the Sahel

Parasitism of Locally Recruited Egg Parasitoids of the Fall Armyworm in Africa

The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an insect native to the tropical and subtropical Americas that has recently spread to Africa, where it predominately attacks maize, sorghum and other plant species. Biological control is an environmentally friendly way of combatting the pest and contributes to an integrated pest management approach. In Africa, several trichogrammatid parasitoids and Telenomus remus Nixon (Hymenoptera: Platygastridae) have been found parasitizing eggs of the FAW. In Niger, the egg parasitoids encountered include Trichogrammatoidea sp. (Hymenoptera: Trichogrammatidae) and Telenomus remus Nixon. Parasitism of the FAW eggs by the two egg parasitoids was assessed in the laboratory, followed by field testing on sentinel eggs. In the laboratory, T. remus parasitized on average 78% of FAWeggs, compared to 25% for Trichogrammatoidea sp. Telenomus remus was able to parasitize egg masses that were fully covered with scales, while Trichogrammatoidea sp. parasitized only uncovered egg masses. On-farm releases of T. remus in sorghum fields caused up to 64% of FAW egg parasitism. Parasitized eggs yielded viable progeny, which can contribute to FAW egg parasitism build-up during the cropping season. Our findings lay the groundwork for the use of T. remus in augmentative releases against FAW in Africa

Development of an Optimum Diet for Mass Rearing of the Rice Moth, Corcyra cephalonica (Lepidoptera: Pyralidae), and Production of the Parasitoid, Habrobracon hebetor (Hymenoptera: Braconidae), for the Control of Pearl Millet Head Miner

The rice moth, Corcyra cephalonica Stainton, an alternate host for the production of the parasitoid, Habrobracon hebetor Say, was reared on different diets, including pearl millet [Pennisetum glaucum (L.) R. Br.] (Poales: Poaceae) flour only, and in combinations of flours of sorghum [Sorghum bicolor (L.) Moench] (Poales: Poaceae), peanut (Arachis hypogea L.) (Fabales: Fabaceae), and cowpea [Vigna unguiculata (L.) Walp.] (Fabales: Fabaceae) to identify the optimal and economical proportion to be used under the conditions of Niger. The addition of cowpea or peanut to the pearl millet diet slightly increased C. cephalonica larval development time. Likewise, the addition of cowpea or peanut to cereal diets yielded a higher C. cephalonica larval survival. Female moths emerging from larvae fed on cereal and legume mixed diets produced higher eggs compared to the ones fed on sole and mixed cereals. Among legumes, cowpea addition is most interesting in terms of cost/production of C. cephalonica larvae. However, female moths emerging from larvae fed on different millet cowpea mix (5, 25, and 50%) laid significantly more eggs than those fed on sole pearl millet. Further, individual C. cephalonica larvae fed on 75% pearl millet + 25% cowpea produced significantly more H. hebetor. With an initial 25 C. cephalonica larvae kept for a 3-mo rearing period, the number of H. hebetor parasitoids produced will reach 2.68–10.07 million. In terms of cost/production ratio, the 75% pearl millet: 25% cowpea yielded better results

How does IPM 3.0 look like (and why do we need it in Africa)?

Open Access Article; Published online: 09 Aug 2022The concept of Integrated Pest Management (IPM) was introduced sixty years ago to curb the overuse of agricultural pesticides, whereby its simplest version (IPM 1.0) was aiming at reducing the frequency of applications. Gradually, agro-ecological principles, such as biological control and habitat management, were included in IPM 2.0. However, throughout this time, smallholder farmers did not improve their decision-making skills and continue to use hazardous pesticides as their first control option. We are therefore proposing a new paradigm — IPM 3.0 — anchored on 3 pillars: 1) real-time farmer access to decision-making, 2) pest-management options relying on science-driven and nature-based approaches, and 3) the integration of genomic approaches, biopesticides, and habitat-management practices. We are convinced that this new paradigm based on technological advances, involvement of youth, gender-responsiveness, and climate resilience will be a game changer. However, this can only become effective through redeployment of public funding and stronger policy support

A Guide to Biological Control of Fall Armyworm in Africa Using Egg Parasitoids

Fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), a voracious agricultural pest native to North and South America, was first detected on the African continent in 2016 and has subsequently spread throughout the continent and across Asia. Fall armyworm (FAW) is known to feed on over 350 plant species and it has been predicted to cause up to $US 13 billion per annum in crop losses throughout sub-Saharan Africa, thereby threatening the livelihoods of millions of poor farmers. Since the occurrence of FAW in Africa, synthetic chemical insecticides have been widely used as emergency responses to halt distribution of the pest and minimize damage in maize fields. Most smallholder farmers in Africa and Asia, however, cannot afford frequent insecticide applications. Furthermore, dependence on chemical insecticides results in the development of resistance to major classes of insecticides, effects on nontarget organisms, as well as other adverse effects to humans and the environment. This highlights the need for the development of integrated pest management (IPM) strategies that are suitable to African smallholder farmers. Biological control using egg parasitoids particularly from the genus Trichogramma and Telenomus remus is part of the IPM approach presently underway to control FAW in North and South America. The approach involves mass rearing and release of these egg parasitoids to control FAW. These egg parasitoids are reared on factitious and natural hosts. Various species of both parasitoids are already present in Africa. After identifying the species/strain that best suit the local condition, the parasitoid wasps can be mass reared and used against FAW and other lepidopteran pests. Therefore, the purpose of this book is to provide guidelines on mass rearing systems for both the egg parasitoids and their hosts. The book describes the methods used to mass produce FAW (S. frugiperda), rice meal moth (Corcyra cephalonica (Stainton), Lepidoptera: Pyralidae), egg parasitoids - (Trichogramma chilonis Ishii, Hymenoptera: Trichogrammatidae) and (Telenomus remus Nixon, Hymenoptera: Platygastridae) in the facilities at icipe- Kenya and ICRISAT-Niger. This guide is primarily intended for biological control practitioners at universities, research institutes and commercial laboratories particularly involved in managing FAW and other lepidopteran pests. The information in this document is also intended to assist those who are relatively new at rearing FAW, rice meal moth, and the parasitoid wasps and to those who wish to improve existing rearing systems. The document covers virtually all aspects of information on the rearing techniques of each species such as colony establishment, stock culture maintenance, diet preparation, mass rearing, storage, quality control and field release. Each section is interrelated, contains step-by-step procedures, and is supported by colour pictures. The guide produced jointly by the International Centre of Insect Physiology and Ecology (icipe), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Haramaya University, and Virginia Tech through support provided by the Feed the Future Innovation Lab for Integrated Pest Management, funded by the of the US Agency for International Development (USAID) under the Cooperative Agreement No. AID-OAA-L-15-00001. International Centre of Insect Physiology and Ecology (icipe) is an international scientific research institute, headquartered in Nairobi, Kenya that works towards improving lives and livelihoods of people in Africa. The center’s main objective is to research and develop alternative and environmentally friendly pest and vector management strategies that are effective, selective, non-polluting, non-resistance inducing, and which are affordable to resource-limited rural and urban communities. icipe's mandate extends to the conservation and use of the rich insect biodiversity found in Africa. Today, icipe is the only international center in sub-Saharan Africa working primarily on arthropods. icipe focuses on sustainable development using human health as the basis and the environment as the foundation for sustainability (http://www.icipe.org/). International Crops Research Institute for the Semi-Arid Tropics (ICRISAT): is a non-profit, non-political organization that conducts agricultural research for development in the drylands of Asia and sub-Saharan Africa. Covering 6.5 million square kilometers of land in 55 countries, the semi-arid or dryland tropics has over 2 billion people, and 644 million of these are the poorest of the poor. ICRISAT and its partners help empower these poor people to overcome poverty, hunger and a degraded environment through better agriculture. ICRISAT is headquartered in Hyderabad, Telangana State, in India, with two regional hubs (Nairobi, Kenya and Bamako, Mali) and country offices in Niger, Nigeria, Zimbabwe, Malawi, Ethiopia and Mozambique. ICRISAT is a member of the CGIAR system Organization (https://www.icrisat.org/). Virginia Polytechnic Institute and State University, commonly known as Virginia Tech, is a public, land-grant research university with its main campus in Blacksburg, Virginia (https://vt.edu/). The university houses the Feed the Future Innovation Lab for Integrated Pest Management, which aims to improve the livelihoods of smallholder farmers by implementing sustainable crop solutions in the developing world. Haramaya University is a public academic and research university with its main campus in Haramaya, located at about 510 km East of Addis Ababa, Ethiopia. The university offers 264 academic programs of which 113 are undergraduate programs, 131 are second degree (M.Sc./M.Ed./MPH) and 20 are PhD level training programs. In addition, the university has been actively involved in research activities, primarily in the fields of agriculture

Timing of releases of the parasitoid Habrobracon hebetor and numbers needed in augmentative biological control against the millet head miner Heliocheilus albipunctella

Heliocheilus albipunctella de Joannis (Lepidoptera: Noctuidae) is one of the major insect pests of pearl millet in the Sahel. The native parasitoid, Habrobracon hebetor Say (Hymenoptera: Braconidae), is currently being promoted for augmentative biological control of the pest in the Sahel. The current study was carried out to identify the right time for releases of the parasitoid using either pearl millet growing stage, or pest occurrence as reference, and to determine the optimal number of parasitoids needed to cover a given area. Our results indicate that release of parasitoids at the panicle emergence stage or six weeks after first sight of eggs of H. albipunctella lead to highest parasitism of H. albipunctella larvae by H. hebetor. The dose of 800 parasitoids for a distance of 3 km radius was enough for controlling H. albipunctella. The implications of the results are discussed toward cost effective and practical recommendation adapted to the Sahelian conditions

The parasitoid Trichogrammatoidea armigera Nagaraja (Hymenoptera: Trichogrammatidae) is a potential candidate for biological control of the millet head miner Heliocheilus albipunctella (de Joannis) (Lepidoptera: Noctuidae) in the Sahel

Pearl millet, Pennisetum glaucum (L.) R. Br., is a crop grown throughout West Africa, especially in the Sahel. Pearl millet is the major staple food for the population of the Sahel, particularly for household use. It is one of the world’s most resilient drought-tolerant cereal crops, surviving even in the poorest soils in the driest regions and in the hottest climates. Despite this extreme climatic adaptation, pearl millet suffers from many biotic constraints, including insect pests (Nwanze and Harris, 1992). Among these, the stem borer (MSB) Coniesta ignefusalis (Hampson) (Lepidoptera: Crambidae) and the millet head miner (MHM) Heliocheilus albipunctella (de Joannis) (Lepidoptera: Noctuidae) are the major chronic insect pests of millet in the Sahel, including Niger. The MSB develops on many species of the Poaceae family; in the Sahel, it develops 2–3 generations per year on pearl millet during the rainy season and diapauses in leftover pearl millet stems during the rest of the year (Youm et al., 1996). The damage from C. ignefusalis is due to the feeding of developing larvae in millet stalks; first generation larvae cause dead hearts and stand loss, while the second and third generations cause lodging, disruption of the vascular system, and inhibition of grain formation (Harris, 1962; Youm et al., 1996). The MHM is a univoltine and monophagous species, which develops on millet in the Sahel during the rainy season between July and October and spends the remainder of the season in diapause in the soil (Gahukar et al., 1986). Infestations of H. albipunctella are more severe in the drier zones of the Sahel (Nwanze and Harris, 1992). The damage from H. albipunctella is due to larvae that feed on the panicle and prevent grain formation (Nwanze and Harris, 1992). Almost every year, outbreaks of the MHM are observed in the Sahel, especially on millet planted early or earlymaturing cultivars, while millet planted later or late-maturing cultivars is more affected by MSB (Gahukar et al., 1986; Youm et al., 1996). Both insect pests inflict significant yield losses ranging from 15% to total crop failure for C. ignefusalis (Harris, 1962; Ajayi, 1990) and from 40% to 85% for H. albipunctella (Gahukar et al., 1986; Krall et al., 1995)..

Heart and en-bloc thymus transplantation in miniature swine

BackgroundDonor-specific tolerance to organ allografts might be induced by cotransplantation of a sufficient amount of vascularized donor thymus. To facilitate donor thymus-induced cardiac allograft tolerance, we have developed a novel technique for heart and en-bloc thymus transplantation in swine.MethodsDonor heart and en-bloc thymus grafts were prepared by a technique that preserves the entire arterial supply and venous drainage of the right thymic lobe. En-bloc grafts (n = 4) were transplanted heterotopically into the abdomens of major histocompatibility complex-matched miniature swine. Recipients received 12 days of cyclosporine intravenously. Grafts were monitored by palpation, electrocardiographic monitoring, and periodic open biopsy. Engraftment of the donor thymus was demonstrated by measuring the proportion of recipient-type thymocytes in the donor thymus with flow cytometry.ResultsAll of the heart and en-bloc thymus grafts had normal cardiac contractility and immediate perfusion of the thymus. All en-bloc grafts were accepted for more than 200 days without significant acute cellular rejection or cardiac allograft vasculopathy. Thymic tissue of en-bloc grafts displayed normal architecture and supported thymopoiesis of recipient-type cells.ConclusionWe have validated a new technique of donor thymus transplantation that could have utility in human heart transplantation

Native parasitoids recruited by the invaded fall army worm in Niger

Surveys of fall army worm Spodoptera frugiperda (J. S. Smith) on maize and sorghum in Niger revealed the occurrence of egg parasitoids (Trichogrammatoidea sp., Trichogramma sp., and Telenomus sp.), egg-larval parasitoids (Chelonus sp.), and larval parasitoids (Cotesia sp., and Charops sp.)