A COMPARISON OF POLLEN GRAINS OF POTENTILLA RECTA L. (ROSACEAE) GROUPS A, B & C IN TURKEY

WOS: 000279569300009

Pollinarium morphology of Vincetoxicum (Apocynaceae: Asclepiadoideae) in Turkey

Coskuncelebi, Kamil/0000-0001-5713-6628; Pinar, Nur Munevver/0000-0001-5466-795XWOS: 000366458500002In this study, the pollen morphology of 20 representatives of ten taxa of Vincetoxicum from Turkey was observed under a scanning electron microscope and light microscope. Observations showed that each flower contained five pollinaria, including a pair of pollinia and caudicles attached to a central corpusculum. in the investigated taxa, the shape of the pollinium varied from ovate, elliptical, and obovate, to clavate, pollen cell surfaces exhibited gemmate or rugulate ornamentation, and the shape of the corpuscula was ovate or oblong. Numerical analysis showed that the shape of pollinia and corpuscula, size and surface ornamentation of pollen cells, and size of corpuscula and caudicles are valuable traits in delimiting the examined taxa. A key to Turkish Vincetoxicum based on pollinarium morphology is presented.RTEUBAPRecep Tayyip Erdogan University [2013.102.03.1]The authors extend their thanks to RTEUBAP (Project number 2013.102.03.1) for the financial support and two anonymous reviewers for comments on a prior version of the manuscript

Nurses' attitudes towards professional containment methods used in psychiatric wards and perceptions of aggression in Turkey

Aims and objective. The aim of this study was to determine nurses' attitudes towards professional containment methods and to explore the relationship between those attitudes and nurses' perception of aggression

Leaf Micromorphology of Some Tanacetum L. (Asteraceae) Taxa in Turkey

WOS: 000418818000003In this study we have examined leaf micromorphology of eight taxa of Tanacetum L. which are distributed in Turkey. The taxa are T. balsamita L. subsp. balsamita, T. balsamita L. subsp. balsamitoides, T. argenteum subsp. flabellifolium, T. argenteum subsp. argenteum, T. argenteum subsp. canum, T. depauperatum, T. haradjanii, T. tomentellum. Although the leaf characteristic has rather limited systematic value, the combination of some of these features could be systematically relevant, especially for the identification of species. According to our findings the leaves of eight taxa are amphistomatic and covering trichomes very frequent and unicellar. Our goal is contribute to taxonomy of Tanatcetum L. which has systematic problems

Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

Pinar, Nur Munevver/0000-0001-5466-795X; SAHIN, AYDAN ACAR/0000-0002-5350-5534WOS: 000439144300010Objective: Cupressaceae pollen has commonly been reported to be an important aeroallergen and causal factor of spring, autumn and winter pollinosis in many countries. The aim of this study was to compare of the structure and allergenic protein content of Cupressus arizonica Greene., Cupressus sempervirens L. and Juniperus oxycedrus L. pollen in detail and contribute to Cupressaceae pollen allergen diagnosis and therapy studies in Turkey. Methods: The pollen structure were examined by LM and SEM. Pollen protein content was investigated by Bradford protein assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot analysis and two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), respectively. Pectate lyase (PL) enzyme activities were compared. Immunoblotting was carried out by using extracts of the three taxa pollen collected from Turkey. Results: All three taxa was found very similar in terms of pollen morphology however, intine thickness was prominently different. Cupressus arizonica pollen extracts showed the lowest PL activity. Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions: As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to improve the Cupressaceae pollen allergen diagnosis and therapy.BAP Research Council in Kirikkale University [2013/26]This study was funded BAP Research Council (Project no: 2013/26) in Kirikkale University. The author thanks Zeynep Misirligil for technical assistance and the Department of Immunology and Allergy Diseases, Ankara University School of Medicine, for giving access to patient sera and data. The authors also thanks to TUBITAK MAM Genetic Engineering and Biotechnology Institute, Kocaeli, Turkey for providing antibodies

A new method to quantify atmospheric Poaceae pollen DNA based on the trnT-F cpDNA region

WOS: 000481592100003Background: Pollen, mold spores, bacteria and viruses are the main biological substances in the atmosphere causing allergic symptoms and disease. Distinguishing pollen and spores is quite time consuming and requires a trained expert. There is a different approach to identification of these substances such as microscopic analysis. However, DNA based identification of these is becoming popular recently. Objective: We evaluated the correlation between the quantity of DNA, which was amplified using trnT-F cpDNA specific primers in samples obtained from a high volume air sampler (HVAS), and concentration of Poaceae pollen collected with a Burkard trap. Materials and methods: Here, we present a method for identifying and quantifying airborne Poaceae pollen using a single step polymerase chain reaction (PCR) technique. Forty daily air samples were collected by HVAS. The method was optimised using two different methods (M1 and M2) and the trnT-F cpDNA region was amplified using a Poaceae specific primer pair. The correlation between the quantity of DNA and pollen concentration was tested using R statistical programming language. Results: Although a significant correlation was obtained between the M1 and M2 methods (R-2 = 0.655, p <0.01), the M2 method was more correlated with pollen concentration. The correlation between pollen and DNA content changed due to episodes that were observed during the pollen season. DNA concentrations from the PCR data were significantly correlated with pollen concentrations determined by light microscopy (R-2 =0.767, p <0.01) in episode II using the M2 method and during the entire season (R-2 =0.469, p <0.01) using M2. Conclusions: The M2 method correctly identified Poaceae pollen in mixed air samples from Zonguldak Province. The non-coding trnT-F cpDNA region was used for the first time in aerobiological samples to identify Poaceae pollen. Use of this method that does not require DNA extraction may be a crucial step for real-time pollen monitoring devices to be developed in the future. The correlation strength between pollen and amplified DNA content could be improved using a sampler that has a lower absorption rate, and a more sensitive technique, such as qPCR.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KBAG-113Z762]This research was partly supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Grant No: KBAG-113Z762