A Wireless Method for Stimulating Characeae Internofal Cells

　車軸藻節間細胞を電気刺激するための，ピエゾ素子を用いる方法を開発した．この方法は電線の接続を要しない非接触のものであるので，遠心顕微鏡観察のもとで細胞を電気刺激することができた．またこの刺激方法は，オジギソウ，アメーバ，ラッパムシにも適用できた

Metabolomics of a Single Vacuole Reveals Metabolic Dynamism in an Alga Chara australis1[W][OA]

Metabolomics is the most reliable analytical method for understanding metabolic diversity in single organelles derived from single cells. Although metabolites such as phosphate compounds are believed to be localized in different organelles in a highly specific manner, the process of metabolite compartmentalization in the cell is not thoroughly understood. The analysis of metabolites in single organelles has consequently presented a significant challenge. In this study, we used a metabolomic method to elucidate the localization and dynamics of 125 known metabolites isolated from the vacuole and cytoplasm of a single cell of the alga Chara australis. The amount of metabolites in the vacuole and the cytoplasm fluctuated asynchronously under various stress conditions, suggesting that metabolites are spatially regulated within the cell. Metabolite transport across the vacuolar membrane can be directly detected using the microinjection technique, which may reveal a previously unknown function of the vacuole