Modeling of leptospirosis outbreaks in relation to hydroclimatic variables in the northeast of Argentina

The transmission of leptospirosis is conditioned by climatic variables. In northeastern Argentina leptospirosis outbreaks occur mainly in coincidence with periods of abundant precipitation and high hydrometric level. A Susceptible-Infectious-Recovered Epidemiological Model (SIR) is proposed, which incorporates hydroclimatic variables for the three most populated cities in the area (Santa Fe, Paraná and Rosario), during the 2009–2018 period. Results obtained by solving the proposed SIR model for the 2010 outbreak are in good agreement with the actual data, capturing the dynamics of the leptospirosis outbreak wave. However, the model does not perform very well in the last months of the year when isolated cases appear outside the outbreak periods, probably due to non- climatic factors not explicitly considered in the present version of the model. Nevertheless, the dynamic modeling of infectious diseases considering hydroclimatic variables constitutes a climatic service for the public health system, not yet available in Argentina.Fil: Gomez, Andrea Alejandra. Universidad Nacional del Litoral. Facultad de Ingenieria y Ciencias Hidricas. Centro de Estudios de Variabilidad y Cambio Climatico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lopez, Maria Soledad. Universidad Nacional del Litoral. Facultad de Ingenieria y Ciencias Hidricas. Centro de Estudios de Variabilidad y Cambio Climatico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Muller, Gabriela Viviana. Universidad Nacional del Litoral. Facultad de Ingenieria y Ciencias Hidricas. Centro de Estudios de Variabilidad y Cambio Climatico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: López, Leonardo Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional; ArgentinaFil: Sione, Walter Fabian. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; ArgentinaFil: Giovanini, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional; Argentin

DETERMINANTES DA ERRADICAÇÃO DA FEBRE AFTOSA NO BRASIL

Foot and mouth disease (FMD) has been a problem for the Brazilian livestock since the XIX century. Once it affects the livestock productivity and has always been a problem for the country exports due to the imposition of sanitary barriers. In 1992, when no state was free from FMD, the National Program for Eradication of FMD (PNEFA) was implemented. Since then good results has been obtained with control and eradication of the disease in some states. However, in other states the problem still persists. It is important to determine why some states have faced difficulties in reaching the status of free from FMD while others not. This work aimed to identify the factors which discriminate the populations of states free from FMD from those not free. The results showed that the private efforts in controlling the disease are the most important variables for differentiating the states free from the non-free ones, together with the lack of public investment. The bad climatic conditions of most of the non-free states seem to contribute to the lack of interest to eradicate the disease. It is suggested attention of the authorities in relation to the state of Alagoas which has presented sufficient scores to be considered free from FMD.foot and mouth disease, Brazil, discriminant analysis, livestock, PNEFA.,

Sugar cane silage as compared to traditional supplemental sources of forage in the performance of high production cows

O experimento foi conduzido com o objetivo de avaliar o desempenho de animais de alta produção alimentados com rações com diferentes fontes de volumosos. Foram availados os seguintes tratamentos: cana-de-açúcar in natura (CA); silagem de cana-de-açúcar inoculada com L. buchneri (SCA); silagem de milho (SM); e mistura de cana-de-açúcar in natura e silagem de milho (CASM). O experimento foi conduzido em quadrado latino 4×4 com períodos de 21 dias, de modo que, nos sete últimos dias, realizou-se a coleta de amostras. Além da avaliação de desempenho, foram feitas análises bromatológicas dos volumosos, das rações e das sobras e análises da composição do leite e da estabilidade aeróbia dos volumosos e das rações. Observaram-se diferenças quanto ao consumo de MS, que foi maior quando os animais foram alimentados com a SCA (23,5 kg/dia) e a CASM (23,5 kg/dia). Todas as rações propiciaram aos animais elevada produção leiteira (24,4 a 25,5 kg/dia), que não diferiu entre os volumosos. A composição do leite variou somente quanto ao teor de gordura, que foi maior quando os animais foram alimentados com SM (36,1%) e CASM (34,8%). Os resultados de estabilidade aeróbia comprovaram efeito positivo da aditivação bacteriana na SCA, que apresentou a maior estabilidade entre os volumosos (13,63 horas). A ração contendo SM como fonte de volumoso apresentou a menor estabilidade, o que provavelmente explica o baixo consumo dos animais nesse tratamento. A SCA é uma alternativa tecnicamente viável à utilização da planta in natura e ambas podem proporcionar elevadas produções de leite, desde que as rações sejam corretamente balanceadas. A inoculação com L. buchneri melhorou o valor nutritivo e reduziu as perdas fermentativas.The experiment was carried out to evaluate the performance of high produce animals fed rations with different sources of forage, what resulted in the treatment: fresh sugarcane (SC), sugarcane silage inoculated with L. buchneri (SCS), corn silage (CS) and mixture of fresh sugarcane and corn silage (SCCS). The experiment was carried out in a 4×4 latin square design with periods of 21 days, and the last 7 days, of each period was used for sample collections. Besides the performance evaluation, chemical analyses of forages, rations and orts were performed and analyses of milk composition and aerobic stability of forages and rations were also made. There was differences for DM intake, which was higher for SCS (23.5 kg/day) and SCSM (23.5 kg/day). All rations provided to the animals high milk production (24.4-25.5 kg/day), which did not differ among the animals. The milk composition varied only as for fat content, which was higher in the animals fed CS (36.1%) and in the SCCS (34.8%). The aerobic stability results showed positive effect of the bacterial aditivation in SCS, which presented the highest aerobic stability among the forages (13.63 hours). The ration with CS as source of forage presented the worst stability, which could explain the lower intake by the animals in this treatment. The SCS is a technical viable alternative to the use of fresh plant and both can proportionate high milk production, as long as, the rations are properly balanced. The inoculation with L. buchneri showed capable to bring benefits for nutritional value and fermentative losses

Effect of moisture absorbents, chemical and microbial additives on the nutritional value, fermentative profile and losses of Brachiaria brizantha cv. Palisadegrass silages

Objetivou-se avaliar o valor nutritivo, o perfil fermentativo e as perdas de silagens de capim-marandu submetidas aos efeitos de absorventes de umidade e aditivos. Utilizou-se o delineamento inteiramente casualizado, em arranjo fatorial 3 × 5, com três teores de matéria seca (obtidos com adição de polpa cítrica (PCP) ou casca de soja (CSP), ambas peletizadas, em comparação à ausência de aditivos) e cinco aditivos (ausência ou presença de inoculante bacteriano, benzoato de sódio ou ácido fórmico 62% ou 44%). O arranjo resultou em 15 silagens, com 4 repetições, portanto 60 silos experimentais. Avaliaram-se o valor nutritivo, as perdas por gases e efluente e a recuperação de matéria seca (MS). A adição de polpa cítrica ou casca de soja peletizada elevou os teores de MS (29,4 e 28,9%), o que reduziu a produção de efluente (4,1 e 3,8 kg/t MV) e disponibilizou substratos fermentescíveis aos microrganismos, fato confirmado pela maior capacidade fermentativa e pela digestibilidade das silagens. Os aditivos à base de ácido fórmico foram mais efetivos em preservar carboidratos solúveis e proteína, o que culminou em maior digestibilidade. Esses aditivos também reduziram as perdas por gases e elevaram a recuperação de MS. O inoculante contendo bactérias homoláticas não diferiu desses aditivos quanto à digestibilidade das silagens e ainda reduziu as perdas por gases. Entre os aditivos, o benzoato foi o menos efetivo em alterar o padrão fermentativo das silagens de capim-marandu. O valor nutritivo e as perdas da silagem com teor de umidade original, não aditivada, podem ser considerados satisfatórios, contudo, a polpa cítrica e principalmente a casca de soja peletizadas se mostraram opção interessante e devem ser mais estudadas. O uso de aditivos contendo ácido fórmico também deve ser mais explorado com gramíneas tropicais, pois promove benefícios ao longo de todo processo fermentativo.The objective of this trial was to evaluate the nutritional value, fermentation profile and dry matter losses of Palisadegrass silages ensiled with either dried citrus pulp, soybean hulls, chemical or microbial additives. The trial was carried out in a completely randomized experimental design and in a factorial arrangement (3 × 5), with three dry matter levels (wet forage or forage ensiled with pelleted citrus pulp or pelleted soybean hulls) and five additives (without or with the presence of bacterial inoculants or the addition of: sodium benzoate, formic acid in the concentration of 62% or 44%), totalizing 15 treatments and 60 experimental silos. The variables analyzed were: nutritional value, losses due to gases and effluents, and dry matter recovery. The use of dried citrus pulp or soybean hulls at the ensiling time increased the dry matter content (29.4 and 28,9%) and decreased the effluent production (4.1 and 3.8 kg/t of fresh matter), also providing fermentable substrate to microorganisms, resulting in increased fermentation coefficient and digestibility of silages. The use of formic acid resulted in silages with higher digestibility and increased water-soluble carbohydrates and crude protein content. This additive was also effective in reducing the losses due to gases and, as a result, increased the total dry matter recovery. The treatment containing homolactic bacteria showed similar trend of increasing the digestibility and reducing the losses due to gases. The use of sodium benzoate was less effective in altering the fermentation pattern of tropical silages. The nutritional value and total dry matter losses of silages ensiled without additives can be considered satisfactory. However, wet forage ensiled with dried citrus pulp and, mainly, with soybeans hulls showed the best results. Treatments containing formic acid had a beneficial effect on the fermentation profile of tropical grass silages.FAPES

Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Reversion or promotion of malignancy by inducing melanogenesis or metastasis

Advanced melanoma is the most aggressive form of skin cancer. It is highlymetastatic and dysfunctional in melanogenesis; two processes that are induced byH2O2. This work presents a melanoma cell model with low levels of H2O2 inducedby catalase overexpression to study differentiation/dedifferentiation processes.Three clones (A7, C10 and G10) of human A375 amelanotic melanoma cells withquite distinct phenotypes were obtained. These clones faced H2O2 scavenging by two main strategies. One developed by clone G10 where ROS increased. This resulted in G10 migration and metastasis associated with the increased of cofilin-1 and CAP1. The other strategy was observed in clone A7 and C10, where ROS levels were maintained reversing malignant features. Particularly, C10 was not tumorigenic, while A7 reversed the amelanotic phenotype by increasing melanin content and melanocytic differentiation markers. These clones allowed the study of potential differentiation and migration markers and its association with ROS levels in vitro and in vivo, providing a new melanoma model with different degree of malignancy.Fil: Bracalente, María Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Investigación y Aplicaciones no Nucleares. Gerencia de Desarrollo Tecnológico y Proyectos Especiales. Departamento de Micro y Nanotecnología; ArgentinaFil: Salguero, Noelia. Comisión Nacional de Energía Atómica. Gerencia de Área de Investigación y Aplicaciones no Nucleares. Gerencia de Desarrollo Tecnológico y Proyectos Especiales. Departamento de Micro y Nanotecnología; ArgentinaFil: Notcovich, Cintia Karina. Comisión Nacional de Energía Atómica. Gerencia de Área de Investigación y Aplicaciones no Nucleares. Gerencia de Desarrollo Tecnológico y Proyectos Especiales. Departamento de Micro y Nanotecnología; ArgentinaFil: Muller, Carolina. Universidade Federal do Rio Grande do Sul; BrasilFil: Da Motta, Leonardo L.. Universidade Federal do Rio Grande do Sul; BrasilFil: Klamt, Fabio. Universidade Federal do Rio Grande do Sul; BrasilFil: Ibañez, Irene Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Investigación y Aplicaciones no Nucleares. Gerencia de Desarrollo Tecnológico y Proyectos Especiales. Departamento de Micro y Nanotecnología; ArgentinaFil: Duran, Hebe Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Investigación y Aplicaciones no Nucleares. Gerencia de Desarrollo Tecnológico y Proyectos Especiales. Departamento de Micro y Nanotecnología; Argentin

Development of cardiorespiratory fitness standards for working memory using receiver operating curves in 15-year-old adolescents

Background: Working memory performance is associated with better academic achievements in children and adolescents, and it is positively related to CRF. However, what level of cardiorespiratory fitness (CRF) discriminates higher working memory performance is not known. The purpose of this study was to identify CRF thresholds linked to working memory in adolescents. Methods: Data of 141 adolescents (53.2 % girls) were collected (14.9 years) from a cross-sectional study during the year 2019. CRF was assessed by the 20-m shuttle run test, and maximal oxygen uptake was calculated using the Mahar ' s equation. Working memory was evaluated by the Corsi blocks test and performance was classified by percentiles. Receiver operating characteristic (ROC) curve analysis was used to identify CRF thresholds. Results: The ROC analysis indicated that CRF could be used to discriminate working memory in adolescents. CRF thresholds of >= 45.03 ml.kg(-1).min(-1)for boys and >= 36.63 ml.kg(-1).min(-1)for girls were found to be indicative of "normal" working memory performance. Conclusions: CRF could discriminate low and normal working memory performance in 14-16- year-old adolescents. These thresholds could allow for earlier identification and intervention of low working memory performance using CRFThe authors would like to thank the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the research productivity grant (ERVR) and the CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for the PhD/MSc scholarship (VMRW, DZF, MROB)

The association of self-reported awake bruxism with anxiety, depression, pain threshold at pressure, pain vigilance, and quality of life in patients undergoing orthodontic treatment

This study aimed to evaluate whether the presence of awake bruxism was associated with temporomandibular dysfunction symptoms, pain threshold at pressure, pain vigilance, oral health-related quality of life (OHRQoL), and anxiety and depression symptoms in patients undergoing orthodontic treatment. Methodology: This observational study followed patients who had started receiving orthodontic treatment for six months. The following variables were measured three times (at baseline, one month, and six months): pressure pain threshold (PPT) in the right and left masseter, anterior temporalis, and temporomandibular joint (TMJ), and right forearm; pain vigilance and awareness questionnaire; and shortened form of the oral health impact profile (OHIP-14). Anxiety and depression symptoms were measured using the Beck anxiety inventory and the Beck depression inventory, respectively. The patients were divided into two main groups according to the presence (n=56) and absence (n=58) of possible awake bruxism. The multi-way analysis of variance (ANOVA) was applied on the date (p=0.050). Results: TMJ and/or muscle pain were not observed in both groups. Time, sex, age group, and awake bruxism did not affect the PPT in the masticatory muscles and pain vigilance (p>0.050). However, the primary effect of awake bruxism was observed when anxiety (ANOVA: F=8.61, p=0.004) and depression (ANOVA: F=6.48, p=0.012) levels were higher and the OHRQoL was lower (ANOVA: F=8.61, p=0.004). Conclusion: The patients with self-reported awake bruxism undergoing an orthodontic treatment did not develop TMJ/masticatory muscle pain. The self-reported awake bruxism is associated with higher anxiety and depression levels and a poorer OHRQoL in patients during the orthodontic treatment

Academic development in technical high school courses in the north region of Brazil: overcoming obstacles and promoting engagement

Continuous development in academic learning can be hindered by rigid rules, resulting in a disconnection between students and the learning process. Promoting socialization and a sense of belonging in the academic environment contributes to student satisfaction. The ability to manage emotions is crucial when facing social challenges, preventing the emergence of emotional instabilities. This article presents a literature review that addresses the teaching and learning process. Based on this review, a structured questionnaire was developed and administered to 108 students from the integrated technical courses at the Tarauacá campus of IFAC, with the purpose of collecting relevant data on their perceptions, opinions, and experiences. Through this process, the psychological consequences of these aspects and their impact on academic performance and learning were aimed to be understood. Upon analyzing the selected studies and the questionnaire results, it is evident that there are practical, cognitive, and emotional challenges that affect students' learning process. To overcome these obstacles, it is important to address issues such as social pressure, differences in learning pace, and inappropriate practices, creating a positive learning environment and adapting pedagogical approaches to students' needs. The constructive approaches proposed in the reviewed studies positively influence students' academic performance, stimulating essential skills and promoting a holistic education. However, it is necessary to rethink the structure of education to keep students motivated, considering issues such as school dropout caused by challenges like remote campus location and financial limitations.Continuous development in academic learning can be hindered by rigid rules, resulting in a disconnection between students and the learning process. Promoting socialization and a sense of belonging in the academic environment contributes to student satisfaction. The ability to manage emotions is crucial when facing social challenges, preventing the emergence of emotional instabilities. This article presents a literature review that addresses the teaching and learning process. Based on this review, a structured questionnaire was developed and administered to 108 students from the integrated technical courses at the Tarauacá campus of IFAC, with the purpose of collecting relevant data on their perceptions, opinions, and experiences. Through this process, the psychological consequences of these aspects and their impact on academic performance and learning were aimed to be understood. Upon analyzing the selected studies and the questionnaire results, it is evident that there are practical, cognitive, and emotional challenges that affect students' learning process. To overcome these obstacles, it is important to address issues such as social pressure, differences in learning pace, and inappropriate practices, creating a positive learning environment and adapting pedagogical approaches to students' needs. The constructive approaches proposed in the reviewed studies positively influence students' academic performance, stimulating essential skills and promoting a holistic education. However, it is necessary to rethink the structure of education to keep students motivated, considering issues such as school dropout caused by challenges like remote campus location and financial limitations