Keefektifan Vaksin Aeromonas Hydrophila Untuk Mengendalikan Penyakit Mas(motile Aeromonas Septicemia) Pada Gurami (Osphronemus Gouramy Lac.) Efficacy of Aeromonas Hydrophila Vaccine to Control Motile Aeromonas Septicemia (Mas) Disease on Gouramy (Osphronemus Gouramy Lac.)

The study was aimed to find an antigen (Ag) O and Ag H of Aeromonas hydrophila vaccines as immunogenic antigen and to evaluate the efficacy of these vaccines to control Motile Aeromonas Septicemia (MAS) disease on gouramy. The research was carried out by Completely Randomized Design with three treatments and five replicates, i.e., Ag O of A. hydrophila vaccine, Ag H of A. hydrophila vaccine, and control. Ten gouramy fishes with length of 10-12 cm and average 8 weight of 28 g were used. Soaking method was used with dosage of 10 for 90 minutes. Booster was conducted a week after vaccination and challenging test was conducted a weeks after booster. Parameters observed were antibody titer, Relative Percent Survival (RPS), survival rate, mean time to death (MTD), and water quality. Data were analyzed by Analysis of Variance followed by Duncan\u27s Multiple Range Test (DMRT). The results showed that Ag O and Ag H of A. hydrophila vaccines could increase antibody titer, survival rate, and RPS (

Fermentasi Tepung Bulu Ayam Dengan Bacillus Licheniformis B2560 Untuk Meningkatkan Kualitas Bahan Baku Pakanikan

- The purpose of this study is to examine fermentation of chicken feather meal with Bacillus licheniformis B2560 to improve the quality of fish feed ingredients. The study was conducted using an experimental method to completely randomized design (CRD) 4 treatments, 3 replications, ie P0 = non- fermented ; P1 = 5 ml inoculum fermentation with B. licheniformis B2560 ; P2 = 10 ml inoculum fermentation with B. licheniformis B2560 ; and P3 = 15 ml inoculum fermentation with B. licheniformis B2560, respectively for chicken feather meal as much as 2 g .Parameters measured were the levels of protein and organoleptic parameters that support, in the form of the physical properties of chicken feather meal, include color, texture, and smell. Data were analyzed using a protein content analysis of variance (ANOVA) with a confidence level of 95 %, followed by Duncans Multiple Range Test Test (DMRT) with a confidence level of 95 % , while for the data results of the organoleptic qualitatively analyzed descriptively.The results showed that the P0 treatment was significantly different (P < 0.05) with P1, P2, and P3, and between treatments P1, P2, and P3 respectively were significantly different (P < 0.05). The treatment gives the best results is the treatment that P1 has the highest protein content of 84.08 % with a change in the physical properties of white -yellow (color), soft (texture), and less typical sting (smell)

Ptk (Penelitian Tindakan Kelas) Dengan Pembelajaran Berbasis Kearifan Lokal Dan Penulisan Artikel Ilmiah Di SD Negeri Kalisube, Banyumas

IbM activity aims to increase knowledge about local knowledge, improve skills develop proposals PTK Based Local Wisdom, compiling reports PTK (class action research) based Local Wisdom, and improve the skills of writing scientific articles. The method used in this activity is participatory learning and action (PLA), include: 1). coordination with partners in preparation for the implementation of the program, contact the team of experts, 2). preparation of several training modules to guide the activities, 3). training local wisdom Banyumas 4). technical training prepare proposals PTK Based Local Wisdom, 5). technical training prepare reports PTK Based Local Wisdom, 6). training technique of writing scientific articles, and 7). mentoring programs and evaluation. The result of this activity is the partner has insight into local wisdom Banyumas, and understand that PTK can be done using local wisdom as PTK Banyumas specific needs and character of students. Partners have the knowledge and skills in the preparation of proposals, the implementation of the PTK in the classroom, and the preparation of reports based on local wisdom PTK, partners have the knowledge and skill in preparing scientific articles, the partners have managed to draw up a report PTK and scientific articles have been published in scientific journals of education. Keywords: scientific articles, Banyumas, local knowledge, PTK (Classroom Action Research), SD Negeri Kalisub

Kandungan Antioksidan Daun Mahang Damar (Macaranga Triloba (Bl.) Muell Arg.)

In the year 2015, the plants that initially grow a lot of wild is increasing in popularity because many contested by the citizens. The reason is because the plant has an economic value. Based on the literature review it is known that apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanolic extract of Macaranga gigantia leaf. Compounds that have potential as antioxidants are generally compounds of flavonoids, phenolics, and alkaloids. Flavonoid and polyphenolic compounds are antioxidant, antidiabetic, anticancer, antiseptic, and anti-inflammatory, while alkaloids have antineoplastic properties that also inhibit the growth of cancer cells. Phenol content, antioxidant activity and antibacterial activity were also found in Macaranga gigantea, M. hosei, M. hypoleuca, M. kingii, M. pruinosa and M. triloba (Lim et.al., 2014). This leads to the notion that mahang leaves were obtained and collected from the Central Kalimantan region where this study carried out also contained similar content and activity. Determination of the value of antioxidant activity in this study using DPPH method performed on n-hexane extract ofmahang leaf obtained by maceration. N-hexane extract of mahang leaf have antioxidant activity, this can be seen from the preliminary TLC test results where the spots produced when sprayed with DPPH fluoresce when viewed at 366 nm uv. The average value of IC50n-hexane extract of mahang leaves was 141.3 ppm

Modifikasi Mesin Reeling Sutera Melalui Penambahan Sistem Sirkulasi Air Panas Otomatis Dan Sistem Pengereman Haspel

Studi ini telah dilakukan dengan mendesain sistem sirkulasi air panas otomatis dan rem haspel sebagai modifikasi pada mesin reeling sutera dengan tujuan untuk konservasi energi melalui peningkatan efisiensi proses. Pada mesin reeling konvensional dapat terjadi inefisiensi pada sistem penyediaan air panas manual yang menyebabkan pelepasan energi panas dan temperatur air yang tidak konstan, serta mekanisme penyambungan benang putus yang dapat mengurangi waktu operasional produktif dan meningkatkan biaya produksi. Metode desain dalam studi ini adalah modifikasi mesin reeling sutera konvensional melalui penambahan sistem sirkulasi air panas otomatis dan sistem pengereman haspel. Berdasarkan uji coba melalui pengoperasian mesin reeling secara kontinyu selama 20 hari, kedua sistem yang dirancang dapat beroperasi dengan baik. Hasil perhitungan analisis ekonomi berdasarkan waktu operasional selama 25 hari memperlihatkan bahwa mesin reeling yang telah dimodifikasi memerlukan biaya produksi benang lebih rendah dan pencapaian waktu break even point yang lebih cepat dibandingkan dengan mesin reeling konvensional. Aplikasi mesin reeling sutera yang telah dimodifikasi ini membutuhkan biaya investasi lebih tinggi, namun demikian produksi benang jauh akan lebih efisien sehingga biaya produksi dalam jangka panjang menjadi lebih rendah dibandingkan dengan mesin reeling konvensional

Spatial and Temporal Variation of Indonesian Throughflow in the Makassar Strait

Using outputs of INDESO model, this study investigated vertical structure, spatial and temporal variation of the Indonesian Throughflow in Makassar Strait (M-ITF). It was shown that the main axis of persistent southward jet of M-ITF formed a unique path following the western shelf slope along the strait, which was associated with a high kinetic energy (KE) region from near-surface down to the thermocline layer. Furthermore, a drastic jump of KE appeared in the narrow and deep Libani Chan-nel (near 3°S) where the strait's width shrinks significantly, thus an elevated flow velocity was needed to maintain transport volume balance. Here, maximum southward velocity at thermocline exceeded 1.2 m/s. Spatial pattern of M-ITF can be described by the first EOF mode which accounts for 79 % of the total variances. It exhibited that contours of the flow amplitudes were similar to M-ITF path, and the largest amplitude was located near the Libani Channel. Out-of-phase relationship of the flow was found between M-ITF and eddies circulation that developed in the edges of the strait. Corresponding temporal fluctuation of the first EOF mode indicated that M-ITF variabilities varied from intra-seasonal to inter-annual scales. Annual fluctuation of M-ITF was seen from EOF mode-2 (at thermocline layer) and mode-3 at lower-thermocline. Cross-spectra analysis revealed that variability of M- ITF (e.g. on annual scale) at northern entrance was highly coherent to the fluctuations of North Equatorial Current (NEC) and Mindanao Current (MC), suggesting that variability of M-ITF was remotely influenced by the Pacific low-latitude western boundary currents

In vitro Study of the Survival, Reproduction and Morphology of Daphnia pulicaria irradiated with a Low Energy Laser

Daphnia is a genus of crustaceans that is representative of freshwater communities. The species exhibit a high sensitivity to a wide range of toxic compounds so that they have been used internationally as biomonitors in toxicity tests to evaluate ecosystem conditions such as water quality. It is also a model genus in genetics, epigenetics and reproductive ecology. In this work, we used Daphnia pulicaria as a model to measure the effects of low-energy laser irradiation on survival, reproduction, and morphology variables of parental organisms and their offspring. We used (1) a single clone line of organisms to eliminate interindividual genetic variability; (2) individuals from more than 50 generations after the clone line was established, and offspring from the third brood onwards to dissipate maternal and epigenetic effects, and (3) neonates, those individuals of the species that have less than 48 hours of life, because they are the most sensitive stage to optical stimuli. We analyzed number of deaths, longevity, age at first reproduction, number of offspring per week, number of total offspring during all their life cycle, body size, size of the antennules, and length of the apical spine of the 4th and 5th brood of the irradiated individuals, who were exposed to a blue laser stimulus of 405 nm for 25 minutes with a power of 40 mW at a distance of 50 cm, compared to those of the control (non-irradiated) group