182 research outputs found
The relationship between truncation and phosphorylation at the C-terminus of tau protein in the paired helical filaments of Alzheimer's disease
Acknowledgements: Authors want to express their gratitude to Dr. P. Davies (Albert Einstein College of Medicine, Bronx, NY, USA) and Lester I. Binder (NorthWestern, Chicago, IL, USA) for the generous gift of mAbs (TG-3, Alz-50, and MC1), and (TauC-3), respectively, and to M. en C. Ivan J. GalvĂĄn-Mendoza for his support in confocal microscopy, and Ms. Maricarmen De Lorenz for her secretarial assistance. We also want to express our gratitude to the Mexican Families who donate the brain of their loved ones affected with Alzheimer's disease, and made possible our research. This work was financially supported by CONACyT grant, No. 142293 (For R.M).Peer reviewedPublisher PD
Cerebrospinal Fluid Cytokines in Multiple System Atrophy: a Cross-Sectional Catalan MSA Registry Study
Introduction: Neuroinflammation is a potential player in neurodegenerative conditions, particularly the aggressive ones, such as multiple system atrophy (MSA). Previous reports on cytokine levels in MSA using serum or cerebrospinal fluid (CSF) have been inconsistent, including small samples and a limited number of cytokines, often without comparison to Parkinson's disease (PD), a main MSA differential diagnosis.
Methods: Cross-sectional study of CSF levels of 38 cytokines using a multiplex assay in 73 participants: 39 MSA patients (19 with parkinsonian type [MSAp], 20 with cerebellar type [MSAc]; 31 probable, 8 possible), 19 PD patients and 15 neurologically unimpaired controls. None of the participants was under non-steroidal anti-inflammatory drugs at the time of the lumbar puncture.
Results: There were not significant differences in sex and age among participants. In global non-parametric comparisons FDR-corrected for multiple comparisons, CSF levels of 5 cytokines (FGF-2, IL-10, MCP-3, IL-12p40, MDC) differed among the three groups. In pair-wise FDR-corrected non-parametric comparisons 12 cytokines (FGF-2, eotaxin, fractalkine, IFN-α2, IL-10, MCP-3, IL-12p40, MDC, IL-17, IL-7, MIP-1ÎČ, TNF-α) were significantly higher in MSA vs. non-MSA cases (PD + controls pooled together). Of these, MCP-3 and MDC were the most significant ones, also differed in MSA vs. PD, and were significant MSA-predictors in binary logistic regression models and ROC curves adjusted for age. CSF levels of fractalkine and MIP-1α showed a strong and significant positive correlation with UMSARS-2 scores.
Conclusion: Increased CSF levels of cytokines such as MCP-3, MDC, fractalkine and MIP-1α deserve consideration as potential diagnostic or severity biomarkers of MSA.info:eu-repo/semantics/publishedVersio
A first approach to assess the impact of bottom trawling over Vulnerable Marine Ecosystems on the High Seas of the Southwest Atlantic
VersiĂłn del edito
Hacia una reforma municipal genuina
El gobierno local es cada vez mĂĄs importante en un entorno cultural de globalizaciĂłn. AhĂ estĂĄ la responsabilidad de gestar las bases de un desarrollo sustentable, de promover la igualdad de oportunidades y de educar al ciudadano en las responsabilidades polĂticas y cĂvicas congruentes con los nuevos retos.
Este trabajo reĂșne la discusiĂłn y reflexiones de investigadores, funcionarios municipales y estatales, analistas y otros interesados en los gobiernos locales que intentan identificar los cambios administrativos, polĂticos, sociales y jurĂdicos para una reforma de los gobiernos locales capaz de resolver los problemas actuales de los municipios y conducir al desarrollo sustentable.ITESO, A.C
Three-dimensional transesophageal echocardiography of the atrial septal defects
Transesophageal echocardiography has advantages over transthoracic technique in defining morphology of atrial structures. Even though real time three-dimensional echocardiographic imaging is a reality, the off-line reconstruction technique usually allows to obtain higher spatial resolution images. The purpose of this study was to explore the accuracy of off-line three-dimensional transesophageal echocardiography in a spectrum of atrial septal defects by comparing them with representative anatomic specimens
Hepatic safety of tipranavir/ritonavir (TPV/r)-based antiretroviral therapy: effect of hepatitis virus co-infection and baseline liver fibrosis
Cistouretropexia laparoscĂłpica tipo Burch para el tratamiento de la incontinencia urinaria de esfuerzos: seguimiento a largo plazo
Objetivos: Conocer las complicaciones, las tasas de cura subjetiva y valoraciĂłn con el Ăndice de severidad de Sandvik a largo plazo de pacientes en quienes se realizĂł cistouretropexia laparoscĂłpica tipo Burch para la correcciĂłn de su incontinencia urinaria de esfuerzo (IUE). MĂ©todo: AnĂĄlisis descriptivo, retrospectivo. Nivel de evidencia III. Se incluyeron las pacientes con diagnĂłstico de IUE tipo Blaivas IIB, a quienes se les practicĂł Burch laparoscĂłpico en la ClĂnica del Prado y Profamilia MedellĂn, entre los años 2003 y 2007. Las variables principales a evaluar fueron edad, tiempo quirĂșrgico, cura subjetiva, Ăndice de severidad de Sandvik y complicaciones. Resultados: Se analizaron 72 pacientes con promedio de edad de 46,57 ± 9,54 años. El tiempo promedio de seguimiento fue de 24,4 meses (rango: 9,0-55,8 meses). El tiempo quirĂșrgico promedio fue de 79,83 minutos (rango: 44-160 minutos). Refirieron cura subjetiva 56 pacientes (77,8%). El Ăndice de severidad de Sandvik muestra que a largo plazo 56,9% pacientes estaban secas con remisiĂłn completa de los sĂntomas y el 13,8% pacientes tenĂan pĂ©rdidas leves. Conclusiones: El Burch laparoscĂłpico es una opciĂłn efectiva para el manejo de la incontinencia urinaria de esfuerzo tipo Blaivas IIB. Provee tasas de curaciĂłn subjetiva a largo plazo similares a otros tipos de tratamiento. Es una opciĂłn mĂĄs a tener en cuenta en el manejo de la IUE, en especial aquellas pacientes con patologĂas asociadas que se beneficien del abordaje por laparoscopia
Confirmation of beach accretion by grain-size trend analysis: Camposoto beach, CĂĄdiz, SW Spain
An application of the grain size trend analysis
(GSTA) is used in an exploratory approach to characterize
sediment transport on Camposoto beach (CĂĄdiz, SW Spain).
In May 2009 the mesotidal beach showed a well-developed
swash bar on the upper foreshore, which was associated
with fair-weather conditions prevailing just before and during
the field survey. The results were tested by means of an
autocorrelation statistical test (index I of Moran). Two sedimentological
trends were recognized, i.e. development towards
finer, better sorted and more negatively skewed
sediment (FBâ), and towards finer, better sorted and less
negatively or more positively skewed sediment (FB+). Both
vector fields were compared with results obtained from
more classical approaches (sand tracers, microtopography
and current measurements). This revealed that both trends
can be considered as realistic, the FB+ trend being identified
for the first time in a beach environment. The data demonstrate
that, on the well-developed swash bar, sediment
transported onshore becomes both finer and better sorted
towards the coast. On the lower foreshore, which exhibits a
steeper slope produced by breaking waves, the higherenergy
processes winnow out finer particles and thereby
produce negatively skewed grain-size distributions. The upper
foreshore, which has a flatter and smoother slope, is
controlled by lower-energy swash-backwash and overwash
processes. As a result, the skewness of the grain-size distributions
evolves towards less negative or more positive
values. The skewness parameter appears to be distributed
as a function of the beach slope and, thus, reflects variations
in hydrodynamic energy. This has novel implications for
coastal management
El grupu neandertal de la Cueva d' El Sidrón (Borines, Piloña)
El artĂculo estĂĄ en la lengua asturianaPeer reviewe
Molecular Tools for Monitoring the Ecological Sustainability of a Stone Bio-Consolidation Treatment at the Royal Chapel, Granada
Background:
Biomineralization processes have recently been applied in situ to protect and consolidate decayed ornamental stone of the Royal Chapel in Granada (Spain). While this promising method has demonstrated its efficacy regarding strengthening of the stone, little is known about its ecological sustainability.Methodology/Principal Findings:
Here, we report molecular monitoring of the stone-autochthonous microbiota before and at 5, 12 and 30 months after the bio-consolidation treatment (medium/long-term monitoring), employing the well-known molecular strategy of DGGE analyses. Before the bio-consolidation treatment, the bacterial diversity showed the exclusive dominance of Actinobacteria (100%), which decreased in the community (44.2%) after 5 months, and Gamma-proteobacteria (30.24%) and Chloroflexi (25.56%) appeared. After 12 months, Gamma-proteobacteria vanished from the community and Cyanobacteria (22.1%) appeared and remained dominant after thirty months, when the microbiota consisted of Actinobacteria (42.2%) and Cyanobacteria (57.8%) only. Fungal diversity showed that the Ascomycota phylum was dominant before treatment (100%), while, after five months, Basidiomycota (6.38%) appeared on the stone, and vanished again after twelve months. Thirty months after the treatment, the fungal population started to stabilize and Ascomycota dominated on the stone (83.33%) once again. Members of green algae (Chlorophyta, Viridiplantae) appeared on the stone at 5, 12 and 30 months after the treatment and accounted for 4.25%, 84.77% and 16.77%, respectively.Conclusions:
The results clearly show that, although a temporary shift in the bacterial and fungal diversity was observed during the first five months, most probably promoted by the application of the bio-consolidation treatment, the microbiota tends to regain its initial stability in a few months. Thus, the treatment does not seem to have any negative side effects on the stone-autochthonous microbiota over that time. The molecular strategy employed here is suggested as an efficient monitoring tool to assess the impact on the stone-autochthonous microbiota of the application of biomineralization processes as a restoration/conservation procedure.This work was supported by the European Regional Development Fund (ERDF), Junta de AndalucĂa (Spain) and the âFortalecimiento de la I+D+iâ program from the University of Granada, co-financed by grant RNM-3493 and Research Group BIO-103 from Junta de AndalucĂa, as well as by the Spanish Government through âJosĂ© Castillejoâ program from the âMinisterio de EducaciĂłn, Cultura y Deporteâ (I+D+i 2008-2011), and by the Austrian Science Fund (FWF) under Grant âElise-Richter V194-B20â
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