Rethinking Ephemeral Architecture. Advanced Geometry for Citizen-Managed Spaces

In recent years there have been a high amount of citizen initiatives that address the complex problems of the contemporary city. There are empty or disused spaces that have been reused for urban gardens, for social use, to encourage integration and civic activities activation, etc. Traditional architectural processes do not provide realistic solutions to these initiatives that, along with limited financial resources, have led to the emergence of architectures and self-constructed facilities, almost as an emergency mode, without necessary planning, media and constructive knowledge. The democratization of technology, thanks to laboratories of digital production, combined with knowledge of the properties of different surfaces through the CAD-CAM tools, offers new opportunities for the development of a lightweight, flexible and low impact architecture, very according to the needs of citizens' initiatives that naturally arise in our cities. The new existing scenario contemplates the figure of the architect, or engineer, not only as an agent of the market, but as a professional able to propose efficient solutions to problems from within, bringing their specific knowledge and serving as bridges between the new technological solutions and the challenges of society

La vida del lado de fuera de las paredes: La manera española de vivir los espacios públicos

The paper analyzes the importance of public spaces as structuring element of sociability and organization of the territory in Spanish cities. Taking as a case study a path and a plaza in the city of Madrid, it aims to explore how these different typologies and scales of public space influence the spatial organization, the mobility and the sociability of these cities. To perform the analysis of these spaces were explored the concepts of imageability, liveability and local management, through the methodologies developed respectively by Lynch (1960), Gehl (2013) and Carmona (2008). The paper looks to comprehend how each typology of public space contributes with local dynamics, and which are the characteristics that should be more studied in order to achieve dynamics public spaces in the city.El paper analiza la importancia de los espacios públicos como elementos estructurantes de la sociabilidad y la organización del territorio en las ciudades españolas. Tomando como caso de estudio un camino y una plaza en la ciudad de Madrid, se pretende explorar cómo esas diferentes tipologías y escalas de espacio público influencian la organización espacial, la movilidad y la sociabilidad de estas ciudades. Para realizar el análisis de estos espacios fueron explorados los conceptos de imageability, liveability y local management, a través de las metodologías desarrolladas respectivamente por Lynch (1960), Gehl (2013) y Carmona (2008). El trabajo quiere comprender cómo cada tipología de espacio público contribuye con la dinámica local, y cuáles son las características que deben ser estudiadas más profundamente a fin de lograr espacios públicos más dinámicos en la ciudad

Repensando la arquitectura efímera. Geometría avanzada para espacios de gestión ciudadana.

En los últimos años se han desarrollado una gran cantidad de iniciativas ciudadanas que hacen frente a los complejos problemas de la ciudad contemporánea. Existen espacios vacíos o en desuso que han sido reutilizados para huertos urbanos, para uso social, para fomentar actividades de integración y activación ciudadana, etc. Los procesos arquitectónicos tradicionales no proporcionan soluciones realistas a estas iniciativas que, junto a los escasos recursos económicos, han propiciado la aparición de unas arquitecturas e instalaciones autoconstruidas, casi a modo de emergencia, sin la planificación, los medios y el conocimiento constructivo necesarios. La democratización de la tecnología, gracias a los laboratorios de fabricación digital, ofrece nuevas oportunidades para el desarrollo de una arquitectura liviana, flexible y de bajo impacto, muy acorde a las necesidades de las iniciativas ciudadanas que van surgiendo de forma natural en nuestras ciudades. El nuevo escenario existente contempla la figura del arquitecto, o del ingeniero, no sólo como un agente al servicio del mercado, sino como un profesional capaz de proponer soluciones eficientes a los problemas desde dentro, aportando su conocimiento específico y sirviendo como puente entre las nuevas soluciones tecnológicas y los retos de la sociedad. En este contexto, el objetivo de este trabajo es proponer nuevas formas de producir arquitectura efímera. Aportando, mediante el conocimiento de la geometría avanzada, el diseño paramétrico y la fabricación digital, herramientas para el desarrollo de nuevos dispositivos arquitectónicos necesarias en los espacios de gestión ciudadana

Immunoassays for scarce tumour-antigens in exosomes: Detection of the human NKG2D-Ligand, MICA, in tetraspanin-containing nanovesicles from melanoma

Abstract Background Tumour-derived exosomes can be released to serum and provide information on the features of the malignancy, however, in order to perform systematic studies in biological samples, faster diagnostic techniques are needed, especially for detection of low abundance proteins. Most human cancer cells are positive for at least one ligand for the activating immune receptor NKG2D and the presence in plasma of NKG2D-ligands can be associated with prognosis. Methods Using MICA as example of a tumour-derived antigen, endogenously expressed in metastatic melanoma and recruited to exosomes, we have developed two immunocapture-based assays for detection of different epitopes in nanovesicles. Although both techniques, enzyme-linked immunosorbent assay (ELISA) and Lateral flow immunoassays (LFIA) have the same theoretical basis, that is, using capture and detection antibodies for a colorimetric read-out, analysis of exosome-bound proteins poses methodological problems that do not occur when these techniques are used for detection of soluble molecules, due to the presence of multiple epitopes on the vesicle. Results Here we demonstrate that, in ELISA, the signal obtained was directly proportional to the amount of epitopes per exosome. In LFIA, the amount of detection antibody immobilized in Au-nanoparticles needs to be low for efficient detection, otherwise steric hindrance results in lower signal. We describe the conditions for detection of MICA in exosomes and prove, for the first time using both techniques, the co-existence in one vesicle of exosomal markers (the tetraspanins CD9, CD63 and CD81) and an endogenously expressed tumour-derived antigen. The study also reveals that scarce proteins can be used as targets for detection antibody in LFIA with a better result than very abundant proteins and that the conditions can be optimized for detection of the protein in plasma. Conclusions These results open the possibility of analyzing biological samples for the presence of tumour-derived exosomes using high throughput techniques.This work has been supported by Grants from Madrid Regional Government [IMMUNOTHERCAN-CM (S2010/BMD-2326)] and the Spanish Ministry of Economy [SAF2015-69169-R (MINEICO/FEDER) and MAT2017-84959-C2-1-R.; the Network of Excellence for Research in Exosomes, Rediex (MINEICO/FEDER); the Consejería de Economía y Empleo del Principado de Asturias (Plan de Ciencia, Tecnología e Innovación 2013-2017), under the Grant GRUPIN14-022. SL-C was a recipient of a FPU fellowship (MECD), and a travel fellowship from the Spanish Group of Extracellular Vesicles (GEIVEX); CC-S was a recipient of a master’s fellowship from “Postgrado de la Fundación Ramón Areces-UAM

Carbon-Coated Superparamagnetic Nanoflowers for Biosensors Based on Lateral Flow Immunoassays

Superparamagnetic iron oxide nanoflowers coated by a black carbon layer (Fe3O4@C) were studied as labels in lateral flow immunoassays. They were synthesized by a one-pot solvothermal route, and they were characterized (size, morphology, chemical composition, and magnetic properties). They consist of several superparamagnetic cores embedded in a carbon coating holding carboxylic groups adequate for bioconjugation. Their multi-core structure is especially efficient for magnetic separation while keeping suitable magnetic properties and appropriate size for immunoassay reporters. Their functionality was tested with a model system based on the biotin–neutravidin interaction. For this, the nanoparticles were conjugated to neutravidin using the carbodiimide chemistry, and the lateral flow immunoassay was carried out with a biotin test line. Quantification was achieved with both an inductive magnetic sensor and a reflectance reader. In order to further investigate the quantifying capacity of the Fe3O4@C nanoflowers, the magnetic lateral flow immunoassay was tested as a detection system for extracellular vesicles (EVs), a novel source of biomarkers with interest for liquid biopsy. A clear correlation between the extracellular vesicle concentration and the signal proved the potential of the nanoflowers as quantifying labels. The limit of detection in a rapid test for EVs was lower than the values reported before for other magnetic nanoparticle labels in the working range 0–3 × 107 EVs/μL. The method showed a reproducibility (RSD) of 3% (n = 3). The lateral flow immunoassay (LFIA) rapid test developed in this work yielded to satisfactory results for EVs quantification by using a precipitation kit and also directly in plasma samples. Besides, these Fe3O4@C nanoparticles are easy to concentrate by means of a magnet, and this feature makes them promising candidates to further reduce the limit of detectionThis work was supported in part by Spanish Ministry of Economy and Competitiveness under projects MAT2017-84959-C2-1-R and the Principality of Asturias (Spain) under project IDI/2018/000185 and the Consellería de Educación Program for Development of a Strategic Grouping in Materials (AEMAT) at the University of Santiago de Compostela under Grant No. ED431E208/08, Xunta de Galicia. Amanda Moyano was supported by a “Severo Ochoa” fellowship (Consejería de Educación y Cultura del Gobierno del Principado de Asturias, grant BP17-152)S

Enseñar en Educación Secundaria, un compromiso con la construcción de aprendizajes relevantes y duraderos. Unidades didácticas, secuencias y proyectos, y sus propuestas de evaluación (2019). Parte 4. Propuestas para Lenguajes y Comunicación

Fil: Ferreyra, Horacio Ademar. Universidad Católica de Córdoba. Facultad de Educación; ArgentinaFil: Vidales, Silvia Noemí. Universidad Católica de Córdoba. Facultad de Educación; Argentin

La renovación de la palabra en el bicentenario de la Argentina : los colores de la mirada lingüística

El libro reúne trabajos en los que se exponen resultados de investigaciones presentadas por investigadores de Argentina, Chile, Brasil, España, Italia y Alemania en el XII Congreso de la Sociedad Argentina de Lingüística (SAL), Bicentenario: la renovación de la palabra, realizado en Mendoza, Argentina, entre el 6 y el 9 de abril de 2010. Las temáticas abordadas en los 167 capítulos muestran las grandes líneas de investigación que se desarrollan fundamentalmente en nuestro país, pero también en los otros países mencionados arriba, y señalan además las áreas que recién se inician, con poca tradición en nuestro país y que deberían fomentarse. Los trabajos aquí publicados se enmarcan dentro de las siguientes disciplinas y/o campos de investigación: Fonología, Sintaxis, Semántica y Pragmática, Lingüística Cognitiva, Análisis del Discurso, Psicolingüística, Adquisición de la Lengua, Sociolingüística y Dialectología, Didáctica de la lengua, Lingüística Aplicada, Lingüística Computacional, Historia de la Lengua y la Lingüística, Lenguas Aborígenes, Filosofía del Lenguaje, Lexicología y Terminología

Carbon-Coated Superparamagnetic Nanoflowers for Biosensors Based on Lateral Flow Immunoassays

Superparamagnetic iron oxide nanoflowers coated by a black carbon layer (Fe3O4@C) were studied as labels in lateral flow immunoassays. They were synthesized by a one-pot solvothermal route, and they were characterized (size, morphology, chemical composition, and magnetic properties). They consist of several superparamagnetic cores embedded in a carbon coating holding carboxylic groups adequate for bioconjugation. Their multi-core structure is especially efficient for magnetic separation while keeping suitable magnetic properties and appropriate size for immunoassay reporters. Their functionality was tested with a model system based on the biotin–neutravidin interaction. For this, the nanoparticles were conjugated to neutravidin using the carbodiimide chemistry, and the lateral flow immunoassay was carried out with a biotin test line. Quantification was achieved with both an inductive magnetic sensor and a reflectance reader. In order to further investigate the quantifying capacity of the Fe3O4@C nanoflowers, the magnetic lateral flow immunoassay was tested as a detection system for extracellular vesicles (EVs), a novel source of biomarkers with interest for liquid biopsy. A clear correlation between the extracellular vesicle concentration and the signal proved the potential of the nanoflowers as quantifying labels. The limit of detection in a rapid test for EVs was lower than the values reported before for other magnetic nanoparticle labels in the working range 0–3 × 107 EVs/μL. The method showed a reproducibility (RSD) of 3% (n = 3). The lateral flow immunoassay (LFIA) rapid test developed in this work yielded to satisfactory results for EVs quantification by using a precipitation kit and also directly in plasma samples. Besides, these Fe3O4@C nanoparticles are easy to concentrate by means of a magnet, and this feature makes them promising candidates to further reduce the limit of detection

Lipid–Polymer Hybrids Encapsulating Iron-Oxide Nanoparticles as a Label for Lateral Flow Immunoassays

The feasibility of using Superparamagnetic Iron Oxide Nanoparticles (SPIONs) encapsulated by lipid–polymer nanoparticles as labels in lateral flow immunoassays (LFIA) was studied. First, nanoparticles were synthesized with average diameters between 4 and 7 (nm) through precipitation in W/O microemulsion and further encapsulated using lipid–polymer nanoparticles. Systems formulated were characterized in terms of size and shape by DLS (Nanozetasizer from Malvern) and TEM. After encapsulation, the average size was around (≈20 and 50 nm). These controlled size agglomerates were tested as labels with a model system based on the biotin–neutravidin interaction. For this purpose, the encapsulated nanoparticles were conjugated to neutravidin using the carbodiimide chemistry, and the LFIA was carried out with a biotin test line. The encapsulated SPIONs showed that they could be promising candidates as labels in LFIA test. They would be useful for immunomagnetic separations, that could improve the limits of detection by means of preconcentration

Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications

Superparamagnetic nanoparticles have seen increased potential in medical and environmental applications. Their preparation is traditionally made by the coprecipitation method, with limited control over the particle size distribution. Microemulsion methods could be advantageous due to the efficient control of the size, shape, and composition of the nanoparticles obtained. Water-in-oil (W/O) microemulsions consist of aqueous microdomains dispersed in a continuous oil phase, stabilized by surfactant molecules. These work as nanoreactors where the synthesis of the desired nanoparticles takes place through a co-precipitation chemical reaction. In this work, superparamagnetic magnetite nanoparticles with average diameters between 5.4 and 7.2 nm and large monodispersity have been synthesized through precipitation in a W/O microemulsion, with Cetyl Trimethyl Ammonium Bromide (CTAB) as a main surfactant, 1-butanol as a cosurfactant, and with 1-hexanol as the continuous oily phase. The optimization of the corresponding washing protocol has also been established since a strict control is required when using these materials for bioapplications. Their applicability in those has been proved by their encapsulation in liposomes, being tested as signal enhancers for lateral flow immunoassays by using the affinity neutravidin-biotin model system. Due to their magnetic behaviour, they were also tested for magnetic separation. These novel materials have been found to be useful for analytical applications requiring high sensitivity and the removal of interferences