Agricultural diffuse pollution in a chalk aquifer (Trois Fontaines, France): Influence of pesticide properties and hydrodynamic constraints

International audienceThe characterization of the transfer of pesticides to and in groundwater is essential for effective water resource management. Intensive monitoring, from October 1989 to May 2006, of a weakly karstified chalk aquifer system in a 50 km2 agricultural catchment, enabled the characterization of the temporal variability of pesticide concentrations in the groundwater of the main outlet. Atrazine and its metabolite deethylatrazine were quantified 394 and 393 times in 476 samples with concentrations ranging from the quantification limit (0.025 μg L−1) to 5.3 and 1.86 μg L−1, respectively. This common presence, compared to the rare detections of isoproturon (in 108 of 476 samples), the pesticide most widely used in the catchment during at least the past decade, highlighted the significant effect of pesticide properties in the time series of concentrations observed in the groundwater. The use of geochemical tracers (nitrate, chloride) analysed in the groundwater and the hydrodynamic monitoring of the system (discharge, water levels) enabled identification of various infiltration mechanisms governing the functioning of the system. The hydrodynamic study showing that the relative contribution of the infiltration mechanisms varies with time, made it possible to explain major variations observed in the pesticide-concentration time series recorded at the spring

Vertical small scale variations of sorption and mineralization of three herbicides in subsurface limestone and sandy aquifer

International audienceVertical variation in sorption and mineralization potential of mecoprop (MCPP), isoproturon and acetochlor were investigated at low concentrations (μg-range) at the cm-scale in unsaturated sub-surface limestone samples and saturated sandy aquifer samples from an agricultural catchment in Brévilles, France. From two intact core drills, four heterogenic limestone sections were collected from 4.50 to 26.40 m below surface (mbs) and divided into 12 sub-samples of 8-25 cm length, and one sandy aquifer section from 19.20 to 19.53 m depth divided into 7 sub-samples of 4-5 cm length. In the sandy aquifer section acetochlor and isoproturon sorption increased substantially with depth; in average 78% (acetochlor) and 61% (isoproturon) per 5 cm. Also the number of acetochlor and isoproturon degraders (most-probable-number) was higher in the bottom half of the aquifer section (93-> 16 000/g) than in the upper half (4-71/g). One 50 cm long limestone section with a distinct shift in color showed a clear shift in mineralization, number of degraders and sorption: In the two brown, uppermost samples, up to 31% mecoprop and up to 9% isoproturon was mineralized during 231 days, the numbers of mecoprop and isoproturon degraders were 1300 to > 16 000/g, and the sorption of both isoproturon and acetochlor was more than three times higher, compared to the two deeper, grayish samples just below where mineralization (≤ 4%) and numbers of degraders (1-520/g) were low for all three herbicides. In both unsaturated limestone and sandy aquifer, variations and even distinct shifts in both mineralization, number of specific degraders and sorption were seen within just 4-15 cm of vertical distance. A simple conceptual model of herbicides leaching to groundwater through a 10 m unsaturated limestone was established, and calculations showed that a 30 cm active layer with the measured sorption and mineralization values hardly impacted the fate of the investigated herbicides, whereas a total thickness of layers of 1 m would substantially increase natural attenuation

Report on the tracer tests (experimental setup, results and interpretation)

Tracer experiments were performed in the Brévilles test site in order to highlight vertical variations in groundwater fluxes related to vertical variations hydraulic conductivity, to estimate contaminant travel time from several locations in the catchment to the springs and to identify transport processes affecting the fate of solutes in the saturated part of the aquifer. Following a first tracer experiment with uranine and sulforhodamine G in 2003, four tracer injections were performed in November 2005 in different piezometers, using uranine, sulforhodamine B, iodide and lithium. Tracer concentrations were monitored in the injection wells and at the basin outlet (spring and gauging station). Using the FVPDM method, concentration evolutions monitored in the injection wells allowed one to estimate local Darcy fluxes. At the basin outlet, only two tracers were recovered. Analyses of breakthrough curves confirm the stratification of the aquifer with more permeable levels in the lower part. They also suggest the probable occurrence of vertical interactions within the aquifer.AquaTerra - Integrated Modelling of the river-sediment-soil-groundwater system; advanced tools for the management of catchment areas and river basins in the context of global chang

Case Report: Central Retinal Artery Occlusion in a COVID-19 Patient.

We report a case of central retinal artery occlusion (CRAO) in a patient with a previous history of severe COVID-19 disease. This disease has been associated with inflammatory-induced homeostasis changes leading to endothelial dysfunction and a procoagulant state with multi-organ involvement, but the burden of thromboembolic complications in COVID-19 patients is currently unknown. The pathogenesis of retinal artery occlusions is a multifactorial process where inflammation and hypercoagulation state are established risk factors. Even if our experience may represent a coincidental relationship, it is likely that COVID-19 patients could be at risk of developing retinal vascular occlusions. A focused ophthalmological surveillance is advisable to prevent and manage this possible cause of severe vision loss that has an important impact in health care system

Prédiction des propriétés de rétention en eau des sols d'un bassin versant à l'aide de fonctions de pédotransfert : influence de la densité apparente et de la teneur en éléments grossiers

L'objectif est d'étudier la qualité de l'estimation des propriétés de rétention en eau faite à l'aide de fonctions de pédotransfert (FPT) pour les sols d'un bassin versant de 250 hectares. Ce bassin versant, situé à proximité de Mantes-la-Jolie, au nord-est de Paris, possède des sols limoneux à limoneux-argileux profonds sur la moitié de sa surface, l'autre moitié correspondant à des sols caillouteux. Après une prospection pédologique détaillée, 15 horizons types ont été définis (Tableau 1), puis 22 unités typologiques de sol (UTS) correspondant à la superposition de plusieurs horizons types et enfin 11 unités cartographiques de sols (UCS) regroupant plusieurs UTS (Tableau 2). Les propriétés de rétention en eau des horizons types ont été déterminées au laboratoire pour 8 valeurs de potentiels variant de –10 à –15000 hPa et, en parallèle, estimées à l'aide des FPT proposées par Hall et al. (1977), Jamagne et al. (1977), Rawls et al. (1982), Vereecken (1989), Bastet (1999) et Bruand et al. (2002) pour des valeurs de potentiel appartenant à ce même domaine. L'aptitude des FPT à rendre compte des variations des propriétés de rétention en eau mesurées pour les horizons types est très variable selon les FPT considérées (Tableau 4). Comme d'autres études l'ont déjà montré (Bastet, 1999, Tietje et Tapkenhinrichs, 1993, Wösten et al., 2001), la proximité géographique de la zone d'étude par rapport à celles ayant servi de base aux FPT et la similitude des matériaux parentaux sont des éléments importants à prendre en compte pour apprécier l'aptitude potentielle de FPT à estimer les propriétés de rétention en eau de sol. Une classification par texture, ou par texture et densité apparente, préalablement à l'établissement de FPT, permet d'améliorer la qualité des estimations. La texture et la densité apparente apparaissent donc comme des grandeurs déterminantes pour estimer correctement les propriétés de rétention en eau des horizons de sols étudiés. Toutefois, la densité apparente à l'échelle de l'horizon peut conduire à prendre en compte un volume de pores qui ne contribuent pas à la réserve en eau du sol, car de trop grande taille, et donc conduire certaines FPT à surestimer largement les valeurs de teneurs en eau. Pour les sols qui possèdent des éléments grossiers, dont la présence n'est pas prise en compte par les FPT, une correction des teneurs en eau mesurées et calculées, visant à les exprimer par rapport à la seule fraction fine (< 2 mm) des horizons, ne permet toutefois à aucune FPT de donner des résultats satisfaisants pour les sols caillouteux. Ces sols caillouteux, le plus souvent peu épais, sont ceux pour lesquels la mesure des propriétés de rétention en eau demeure la plus difficile à obtenir, aucune méthodologie adaptée n'étant disponible à l'heure actuelle

Two dechlorinated chlordecone derivatives formed by in situ chemical reduction are devoid of genotoxicity and mutagenicity and have lower proangiogenic properties compared to the parent compound

Chlordecone (CLD) is a chlorinated hydrocarbon insecticide, now classified as a persistent organic pollutant. Several studies have previously reported that chronic exposure to CLD leads to hepatotoxicity, neurotoxicity, raises early child development and pregnancy complications, and increases the risk of liver and prostate cancer. In situ chemical reduction (ISCR) has been identified as a possible way for the remediation of soils contaminated by CLD. In the present study, the objectives were (i) to evaluate the genotoxicity and the mutagenicity of two CLD metabolites formed by ISCR, CLD-5a-hydro, or CLD-5-hydro (5a- or 5- according to CAS nomenclature; CLD-1Cl) and tri-hydroCLD (CLD-3Cl), and (ii) to explore the angiogenic properties of these molecules. Mutagenicity and genotoxicity were investigated using the Ames\u27s technique on Salmonella typhimurium and the in vitro micronucleus micromethod with TK6 human lymphoblastoid cells. The proangiogenic properties were evaluated on the in vitro capillary network formation of human primary endothelial cells. Like CLD, the dechlorinated derivatives of CLD studied were devoid of genotoxic and mutagenic activity. In the assay targeting angiogenic properties, significantly lower microvessel lengths formed by endothelial cells were observed for the CLD-3Cl-treated cells compared to the CLD-treated cells for two of the three tested concentrations. These results suggest that dechlorinated CLD derivatives are devoid of mutagenicity and genotoxicity and have lower proangiogenic properties than CLD

Language attitudes towards Serbian Sign Language and experiences with deaf education in Serbia

In this paper, we report on data obtained from interviews with 28 deaf signers and three hearing teachers of deaf pupils regarding their experiences with and attitudes towards deaf education and Serbian Sign Language (SZJ). Following transcription of the data, we conducted thematic analyses of the deaf informants’ and teachers’ comments. Data, which indicate that a change in language attitudes among deaf people has taken place compared to the oralist ideology still prevalent among teachers, are considered within a broader historical context by giving the first account of SZJ, its place in education and its history. In the light of the results, we highlight the importance of teacher training for promoting a cultural-linguistic minority perspective in deaf education in Serbia that would allow for deafness to be viewed as human diversity rather than disability

Clinical Outcomes and Cataract Formation Rates in Eyes 10 Years After Posterior Phakic Lens Implantation for Myopia.

Intraocular collamer lenses (ICLs) are posterior chamber phakic lenses that provide a refractive surgery option for those with high myopia or astigmatism. The short-term and midterm results indicate good refraction stability, efficacy, and safety. Cataract has been suggested to be an important long-term complication of ICL implantation. To report the rates of cataract development and refractive outcomes 10 years after ICL implantation. The study included 133 eyes of 78 patients undergoing consecutive V4 model ICL implantations, which took place from January 1, 1998, through December 31, 2004, at Jules-Gonin Eye Hospital, Lausanne, Switzerland. Data analysis was performed from January 1, 2014, to May 31, 2014. The lenses implanted were as follows: 53 V4 model ICLs of -15.5 D or greater, 73 V4 model ICLs of less than -15.5 diopter (D), and 7 V4 model toric ICLs for myopia. Rate of cataract surgery, lens opacity, ocular hypertension, refractive safety, predictability, and stability. A total of 133 eyes of 78 patients (34 men and 44 women, with a mean [SD] age of 38.8 [9.2] years at enrollment) met the inclusion criteria. The rate of lens opacity development was 40.9% (95% CI, 32.7%-48.8%) and 54.8% (95% CI, 44.7%-63.0%) at 5 and 10 years, respectively. Phacoemulsification was performed in 5 eyes (4.9%; 95% CI, 1.0%-8.7%) and 18 eyes (18.3%; 95% CI, 10.1%-25.8%) at 5 and 10 years after ICL implantation, respectively. The vault height (distance between the posterior ICL surface and anterior lens surface) measured a mean (SD) of 426 (344) μm immediately postoperatively, decreasing to 213 (169) μm at 10 years. A smaller vault height was associated with the development of lens opacity and phacoemulsification (P = .005 and .008, respectively). The intraocular pressure was 15 mm Hg postoperatively, and there was no significant increase in intraocular pressure observed until the 10-year follow-up (16 mm Hg, P = .02). At 10 years, 12 eyes (12.9%; 95% CI, 5.6%-19.6%) had developed ocular hypertension that required topical medication. At 10 years, the mean (SD) safety index was 1.25 (0.57), with a manifest spherical equivalent of -0.5 D at 1-year postoperatively vs -0.7 D at 10 years postoperatively in eyes aimed at emmetropia. This retrospective single center study indicates that ICL implantation provides good long-term safety and stability of refraction in patients with high myopia compared with similar short-term studies. However, the rates of cataract formation and ocular hypertension at 10 years have important clinical implications, and as such this information should be part of the available patient information before ICL implantation

Natural transformation of chlordecone into 5b-hydrochlordecone in French West Indies soils: statistical evidence for investigating long-term persistence of organic pollutants

International audienceChlordecone (CLD) was an organochlorine insecticide whose previous use resulted in an extensive pollution of the environment with severe health effects and social consequences. A closely related compound, 5b-hydrochlordecone (5b-hydroCLD), has been searched for and often detected in environmental matrices from the geographical area where CLD was applied. The current consensus considered that its presence was not the result of a biotic or abiotic dechlorination of CLD in these matrices but rather the consequence of its presence as impurity (synthesis by-product) in the CLD released into the environment. The aim of the present study was to determine if and to what extent degradation of CLD into 5b-hydroCLD occurred in the field. To test this hypothesis, the ratios of 5b-hydroCLD and CLD concentrations in a dataset of 810 soils collected between 2006 and 2012 in Martinique were compared to the ratios measured in 3 samples of the CLD dust commercial formulations applied in the banana fields of French West Indies (FWI) and 1 sample of the technical-grade CLD corresponding to the active ingredient used in such formulations. Soil data were processed with a hierarchical Bayesian model to account for random measurement errors and data censoring. Any pathway of CLD transformation into 5b-hydroCLD occurring over the long term in FWI soils would indeed change the ratio of 5b-hydroCLD/CLD compared to what it was in the initially applied formulations. Results showed a significant increase of the 5b-hydroCLD/CLD ratio in the soils-25 times greater in soil than in commercial formulations-which suggested that natural CLD transformation into 5b-hydroCLD over the long term occurred in these soils. Results from this study may impact future decisions for the remediation of the polluted areas