860 research outputs found
High VEGF with Rapid Growth and Early Metastasis in a Mouse Osteosarcoma Model
A murine model of osteosarcoma was developed to investigate the association between the expression of VEGF and the progression of osteosarcoma. Two human osteosarcoma cell lines with distinct VEGF expressions were introduced into proximal tibiae of immuno-deficient SCID mice, either by direct injection through the cortical bone or surgical exposing and drilling on the tibial metaphysis to seed tumor cells. Bone tumors were obvious on microCT within 4 weeks following osteosarcoma cell inoculation through surgical delivery. In contrast, direct injection without drilling often resulted in periosteal tumors. Although neoplasms were developed regardless of VEGF levels, orthotopic tumors derived from high VEGF-expressing cells were detected 2 weeks earlier on CT images than the ones from VEGF negative cells. At sacrifice, high VEGF tumors were distinctively larger in size and more frequently invaded the adjacent bone tissue. Multiple metastatic lesions were found in all the lung tissues at 8 weeks from high VEGF group, whereas only 1 of 7 VEGF negative tumors exhibited pulmonary metastasis. Overall, this model developed with the surgical tumor cell delivery results in histological and radiographic features more consistent with primary osteosarcoma. Interestingly, VEGF expression correlates with the early establishment, rapid tumor growth, and the development of pulmonary metastasis
Surface polaritons on left-handed cylinders: A complex angular momentum analysis
We consider the scattering of electromagnetic waves by a left-handed cylinder
-- i.e., by a cylinder fabricated from a left-handed material -- in the
framework of complex angular momentum techniques. We discuss both the TE and TM
theories. We emphasize more particularly the resonant aspects of the problem
linked to the existence of surface polaritons. We prove that the long-lived
resonant modes can be classified into distinct families, each family being
generated by one surface polariton propagating close to the cylinder surface
and we physically describe all the surface polaritons by providing, for each
one, its dispersion relation and its damping. This can be realized by noting
that each surface polariton corresponds to a particular Regge pole of the
matrix of the cylinder. Moreover, for both polarizations, we find that there
exists a particular surface polariton which corresponds, in the large-radius
limit, to the surface polariton which is supported by the plane interface.
There exists also an infinite family of surface polaritons of
whispering-gallery type which have no analogs in the plane interface case and
which are specific to left-handed materials.Comment: published version. v3: reference list correcte
Low field vortex dynamics over seven time decades in a Bi_2Sr_2CaCu_2O_{8+\delta} single crystal for temperatures 13 K < T < 83 K
Using a custom made dc-SQUID magnetometer, we have measured the time
relaxation of the remanent magnetization M_rem of a Bi_2Sr_2CaCu_2O_{8+\delta}
single crystal from the fully critical state for temperatures 13 K < T < 83 K.
The measurements cover a time window of seven decades 10^{-2} s < t < 10^5 s,
so that the current density j can be studied from values very close to j_c down
to values considerably smaller than j_c. From the data we have obtained: (i)
the flux creep activation barriers U as a function of current density j, (ii)
the current-voltage characteristics E(j) in a typical range of 10^{-7} V/cm to
10^{-15} V/cm, and (iii) the critical current density j_c(0) at T = 0. Three
different regimes of vortex dynamics are observed: For temperatures T < 20 K
the activation barrier U(j) is logarithmic, no unique functional dependence
U(j) could be found for the intermediate temperature interval 20 K < T < 40 K,
and finally for T > 40 K the activation barrier U(j) follows a power-law
behavior with an exponent mu = 0.6. From the analysis of the data within the
weak collective pinning theory for strongly layered superconductors, it is
argued that for temperatures T < 20 K pancake-vortices are pinned individually,
while for temperatures T > 40 K pinning involves large collectively pinned
vortex bundles. A description of the vortex dynamics in the intermediate
temperature interval 20 K < T < 40 K is given on the basis of a qualitative low
field phase diagram of the vortex state in Bi_2Sr_2CaCu_2O_{8+\delta}. Within
this description a second peak in the magnetization loop should occur for
temperatures between 20 K and 40 K, as it has been observed in several
magnetization measurements in the literature.Comment: 12 pages, 10 figure
Regulation of neutrophil senescence by microRNAs
Neutrophils are rapidly recruited to sites of tissue injury or infection, where they protect against invading pathogens. Neutrophil functions are limited by a process of neutrophil senescence, which renders the cells unable to respond to chemoattractants, carry out respiratory burst, or degranulate. In parallel, aged neutrophils also undergo spontaneous apoptosis, which can be delayed by factors such as GMCSF. This is then followed by their subsequent removal by phagocytic cells such as macrophages, thereby preventing unwanted inflammation and tissue damage. Neutrophils translate mRNA to make new proteins that are important in maintaining functional longevity. We therefore hypothesised that neutrophil functions and lifespan might be regulated by microRNAs expressed within human neutrophils. Total RNA from highly purified neutrophils was prepared and subjected to microarray analysis using the Agilent human miRNA microarray V3. We found human neutrophils expressed a selected repertoire of 148 microRNAs and that 6 of these were significantly upregulated after a period of 4 hours in culture, at a time when the contribution of apoptosis is negligible. A list of predicted targets for these 6 microRNAs was generated from http://mirecords.biolead.org and compared to mRNA species downregulated over time, revealing 83 genes targeted by at least 2 out of the 6 regulated microRNAs. Pathway analysis of genes containing binding sites for these microRNAs identified the following pathways: chemokine and cytokine signalling, Ras pathway, and regulation of the actin cytoskeleton. Our data suggest that microRNAs may play a role in the regulation of neutrophil senescence and further suggest that manipulation of microRNAs might represent an area of future therapeutic interest for the treatment of inflammatory disease
Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall
Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape. The extent of these shape changes is limited by the mechanical properties of the cells, which will be largely dependent on the structure of the cell walls. Although it has long been observed that guard cells are anisotropic due to differential thickening and the orientation of cellulose microfibrils, our understanding of the composition of the cell wall that allows them to undergo repeated swelling and deflation remains surprisingly poor. Here, we show that the walls of guard cells are rich in unesterified pectins. We identify a pectin methylesterase gene, PME6, which is highly expressed in guard cells and required for stomatal function. pme6-1 mutant guard cells have walls enriched in methyl-esterified pectin and show a decreased dynamic range in response to triggers of stomatal opening/closure, including elevated osmoticum, suggesting that abrogation of stomatal function reflects a mechanical change in the guard cell wall. Altered stomatal function leads to increased conductance and evaporative cooling, as well as decreased plant growth. The growth defect of the pme6-1 mutant is rescued by maintaining the plants in elevated CO2, substantiating gas exchange analyses, indicating that the mutant stomata can bestow an improved assimilation rate. Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, stomatal function, and plant growth. Our results establish a link between gene expression in guard cells and their cell wall properties, with a corresponding effect on stomatal function and plant physiology
Importance of Polaronic Effects for Charge Transport in CdSe Quantum Dot Solids
We developed an accurate model accounting for electron-phonon interaction in colloidal
quantum dot supercrystals that allowed us to identify the nature of charge carriers and the
electrical transport regime. We find that in experimentally analyzed CdSe nanocrystal solids the electron-phonon interaction is sufficiently strong that small polarons localized to single dots are formed. Charge-carrier transport occurs by small polaron hopping between the dots, with mobility that decreases with increasing temperature. While such a temperature dependence of mobility is usually considered as a proof of band transport, we show that the same type of dependence occurs in the system where transport is dominated by small polaron hopping
ACCESS: Design and Sub-System Performance
Establishing improved spectrophotometric standards is important for a broad range of missions and is relevant to many astrophysical problems. ACCESS, "Absolute Color Calibration Experiment for Standard Stars", is a series of rocket-borne sub-orbital missions and ground-based experiments designed to enable improvements in the precision of the astrophysical flux scale through the transfer of absolute laboratory detector standards from the National Institute of Standards and Technology (NIST) to a network of stellar standards with a calibration accuracy of 1% and a spectral resolving power of 500 across the 0.35 -1.7 micrometer bandpass
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