Cryo-EM reveals the conformation of a substrate analogue in the human 20S proteasome core

The proteasome is a highly regulated protease complex fundamental for cell homeostasis and controlled cell cycle progression. It functions by removing a wide range of specifically tagged proteins, including key cellular regulators. Here we present the structure of the human 20S proteasome core bound to a substrate analogue inhibitor molecule, determined by electron cryo-microscopy (cryo-EM) and single-particle analysis at a resolution of around 3.5 Å. Our map allows the building of protein coordinates as well as defining the location and conformation of the inhibitor at the different active sites. These results open new prospects to tackle the proteasome functional mechanisms. Moreover, they also further demonstrate that cryo-EM is emerging as a realistic approach for general structural studies of protein–ligand interactions

Experimental study of laboratory-heated CM2 chondrites Mighei and Murchison

We conducted experimental heating of two CM2 chondrites, Murchison and Mighei, to study changes in their oxygen isotopic compositions and mineralogy and explore possible genetic relationships between MCCs and normal CMs

A Review of Trimen’s False Tiger Moth, Agoma trimenii (Lepidoptera: Agaristidae): Seasonal Biology, Potential Monitoring and Control Techniques

Trimen’s false tiger moth, Agoma trimenii (Lepidoptera: Agaristidae), has recently been found to occur in vineyards in the Northern Cape and Limpopo (Groblersdal area) provinces of South Africa. As little is known about the biology and behaviour of the moth, no official monitoring methods or economic thresholds relating to it, exist. Consequently, management and registered control options still require development. The first aim in the current review, was to gather and critically discuss all the available information on A. trimenii in the context of the information gained from field observations conducted in the Northern Cape, South Africa, during the 2016/2017 and 2017/2018 seasons. The paper also includes reporting on field observations made with regard to various aspects of the seasonal life cycle and ecology of A. trimenii, with a view to investigate, in future research, the potential biological control options available. Potential monitoring strategies of A. trimenii in the field were investigated. Various life stages of A. trimenii were identified, peak flight times were established, overlapping generations were determined, and the behavioural traits of all life stages were documented. Ultraviolet blue light traps proved to be the most promising potential monitoring strategy, with the prospect for an A. trimenii pheromone lure holding potential as an alternative monitoring strategy in future. With summarising all current information on A. trimenii, recommendations for growers to monitor and control A. trimenii are presented, towards the development of an integrated pest management system for the moth

Stepped vitrification technique for human ovarian tissue cryopreservation

The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation. Ovarian cortex was taken from 7 fertile adult women. Samples were subjected to an SV protocol performed in an automatic freezer, which allowed sample transfer to ever higher concentrations of dimethyl sulfoxide (DMSO) as the temperature was reduced. Histological evaluation of the vitrified-warmed tissue showed large numbers of degenerated follicles after 24 hours of in vitro culture. We therefore evaluated DMSO perfusion rates by X-ray computed tomography, ice crystal formation by freeze-substitution, and cell toxicity by transmission electron microscopy, seeking possible reasons why follicles degenerated. Although cryoprotectant perfusion was considered normal and no ice crystals were formed in the tissue, ultrastructural analysis detected typical signs of DMSO toxicity, such as mitochondria degeneration, alterations in chromatin condensation, cell vacuolization and extracellular matrix swelling in both stromal and follicular cells. The findings indicated that the method failed to preserve follicles due to the high concentrations of DMSO used. However, adaptations can be made to avoid toxicity to follicles caused by elevated levels of cryoprotectants.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 2016/22947-

Super black hole as spinning particle: Supersymmetric baglike core

We consider particlelike solutions to supergravity based on the Kerr-Newman black hole (BH) solution. The BH singularity is regularized by means of a phase transition to a new vacuum state near the core region confining a dual gauge field. Supersymmetric BPS-saturated domain wall model is suggested which can provide this phase transition and formation the stable charged superconducting core. For spinning particle the core takes the form of thin, relativistically rotaiting disk.Comment: 9 pages, Latex, plenary talks given at the School-Workshop Praha-Spin-2001 (Prague,July 15-28,2001) and at the XXIV International Workshop on Fundamental Problems of HEP and Field Theory (IHEP, June 2001, Protvino

Interacting dark matter contribution to the Galactic 511 keV gamma ray emission: constraining the morphology with INTEGRAL/SPI observations

We compare the full-sky morphology of the 511 keV gamma ray excess measured by the INTEGRAL/SPI experiment to predictions of models based on dark matter (DM) scatterings that produce low-energy positrons: either MeV-scale DM that annihilates directly into e+e- pairs, or heavy DM that inelastically scatters into an excited state (XDM) followed by decay into e+e- and the ground state.By direct comparison to the data, we find that such explanations are consistent with dark matter halo profiles predicted by numerical many-body simulations for a Milky Way-like galaxy. Our results favor an Einasto profile over the cuspier NFW distribution and exclude decaying dark matter scenarios whose predicted spatial distribution is too broad. We obtain a good fit to the shape of the signal using six fewer degrees of freedom than previous empirical fits to the 511 keV data. We find that the ratio of flux at Earth from the galactic bulge to that of the disk is between 1.9 and 2.4, taking into account that 73% of the disk contribution may be attributed to the beta decay of radioactive 26Al.Comment: 7 pages, 4 figures. Includes minor corrections, and a discussion of threshold energies in XDM models. Published in JCA

Thermodynamics of non-local materials: extra fluxes and internal powers

The most usual formulation of the Laws of Thermodynamics turns out to be suitable for local or simple materials, while for non-local systems there are two different ways: either modify this usual formulation by introducing suitable extra fluxes or express the Laws of Thermodynamics in terms of internal powers directly, as we propose in this paper. The first choice is subject to the criticism that the vector fluxes must be introduced a posteriori in order to obtain the compatibility with the Laws of Thermodynamics. On the contrary, the formulation in terms of internal powers is more general, because it is a priori defined on the basis of the constitutive equations. Besides it allows to highlight, without ambiguity, the contribution of the internal powers in the variation of the thermodynamic potentials. Finally, in this paper, we consider some examples of non-local materials and derive the proper expressions of their internal powers from the power balance laws.Comment: 16 pages, in press on Continuum Mechanics and Thermodynamic

Revisiting consistency conditions for quantum states of systems on closed timelike curves: an epistemic perspective

There has been considerable recent interest in the consequences of closed timelike curves (CTCs) for the dynamics of quantum mechanical systems. A vast majority of research into this area makes use of the dynamical equations developed by Deutsch, which were developed from a consistency condition that assumes that mixed quantum states uniquely describe the physical state of a system. We criticise this choice of consistency condition from an epistemic perspective, i.e., a perspective in which the quantum state represents a state of knowledge about a system. We demonstrate that directly applying Deutsch's condition when mixed states are treated as representing an observer's knowledge of a system can conceal time travel paradoxes from the observer, rather than resolving them. To shed further light on the appropriate dynamics for quantum systems traversing CTCs, we make use of a toy epistemic theory with a strictly classical ontology due to Spekkens and show that, in contrast to the results of Deutsch, many of the traditional paradoxical effects of time travel are present.Comment: 10 pages, 6 figures, comments welcome; v2 added references and clarified some points; v3 published versio

Classical and Quantum Strings in compactified pp-waves and Godel type Universes

We consider Neveu-Schwarz pp-waves with spacetime supersymmetry. Upon compactification of a spacelike direction, these backgrounds develop Closed Null Curves (CNCs) and Closed Timelike Curves (CTCs), and are U-dual to supersymmetric Godel type universes. We study classical and quantum strings in this background, with emphasis on the strings winding around the compact direction. We consider two types of strings: long strings stabilized by NS flux and rotating strings which are stabilized against collapse by angular momentum. Some of the latter strings wrap around CNCs and CTCs, and are thus a potential source of pathology. We analyze the partition function, and in particular discuss the effects of these string states. Although our results are not conclusive, the partition function seems to be dramatically altered due to the presence of CNCs and CTCs. We discuss some interpretations of our results, including a possible sign of unitary violation.Comment: 42 pages, LaTeX, 2 figure

The implications of service quality gaps for strategy implementation

This article addresses the problem of service quality strategy implementation and proposes three interrelated models: a static model of the organisation; a comprehensive dynamic model of the implementation process, both synthesised from the literature; and a mixed model, which integrates static and dynamic models. The mixed model is combined with the service quality gaps (SQGs) model, drawn at a previous congress paper, to propose a map of the pattern of SQGs occurring at each implementation stage; the organisational variables that can be manipulated to eliminate SQGs; and several implications to practising managers