Extraordinary Writs: A Powerful Tool for the Florida Practitioner

This article explores the distinctive nature of extraordinary writs and their availability in the Florida courts. Recent decisional and statutory law in this area is analyzed in detail. The authors\u27 presentation affords Florida practitioners valuable guidelines for the effective invocation of extraordinary writs

Investigating what level of visual information inspires trust in a user of a highly automated vehicle

The aim of this research is to investigate whether visual feedback alone can affect a driver’s trust in an autonomous vehicle, and in particular, what level of feedback (no feedback vs. moderate feedback vs. high feedback) will evoke the appropriate level of trust. Before conducting the experiment, the Human Machine Interfaces (HMI) were piloted with two sets of six participants (before and after iterations), to ensure the meaning of the displays can be understood by all. A static driving simulator experiment was conducted with a sample of 30 participants (between 18 and 55). Participants completed two pre-study questionnaires to evaluate previous driving experience, and attitude to trust in automation. During the study, participants completed a trust questionnaire after each simulated scenario to assess their trust level in the autonomous vehicle and HMI displays, and on intention to use and acceptance. The participants were shown 10 different driving scenarios that lasted approximately 2 minutes each. Results indicated that the ‘high visual feedback’ group recorded the highest trust ratings, with this difference significantly higher than for the ‘no visual feedback’ group (U = .000; p = <0.001 < α) and the ‘moderate visual feedback’ group (U = .000; p = <0.001 < α). There is an upward inclination of trust in all groups due to familiarity to both the interfaces and driving simulator over time. Participants’ trust level was also influenced by the driving scenario, with trust reducing in all displays during safety verses non-safety-critical situations

Screening for post-traumatic stress symptoms in looked after children

Purpose: Many children who are looked after by the state have experienced adverse and traumatic life circumstances prior to being removed from their biological parents. Previous research has highlighted that many of them experience barriers to accessing psychological therapies. The purpose of this paper is to investigate the feasibility of assessing post-traumatic stress disorder (PTSD)-like symptoms using a screening tool, and through this to determine the prevalence of PTSD-like symptoms in looked after children presenting with emotional and/or behavioural problems.   Design/methodology/approach: The Child Revised Impact of Events Scale (CRIES-8) was identified as a suitable screening tool for PTSD-like symptoms. This measure was piloted for three months, and the prevalence of PTSD-like symptoms amongst respondents ( n=27) was recorded.   Findings: Prevalence of PTSD-like symptoms was found to be high 75 per cent amongst respondents. The psychometric properties of the CRIES-8 were similar to those found in a previous study assessing PTSD following a single-incident trauma. Health care professionals reported finding the CRIES-8 to be a clinically useful measure.   Originality/value: Prevalence of PTSD-like symptoms may be high amongst looked after children, and the CRIES-8 appears to have good psychometric properties when used with this population. It is likely that this highly treatable condition is under-detected: thus, recommendations are made for clinical practice and further research

Smart Systems and Collaborative Innovation Networks for Productivity Improvement in SMEs

The adoption of Smart Manufacturing Systems in manufacturing companies is often seen as a strategy towards achieving improvements in productivity. However, there is little evidence to indicate that UK manufacturing SMEs are prepared for the implementation of such systems. Through the employment of a triangulation research approach involving the detailed examination of 36 UK manufacturing SMEs from three manufacturing sectors, this study investigates the level of awareness and understanding within SMEs of Smart Manufacturing Systems. The development of a profiling tool is shown and is subsequently used to audit company awareness and understanding of the key technologies, collaborative networks and systems of SMS. Further information obtained from semi-structured interviews and observations of manufacturing operations provide further contextual information. The findings indicate that whilst the priority technologies and systems differ between manufacturing sectors, the key issues around the need for developing appropriate collaborative networks and knowledge management systems are common to all sectors

Avian Resistance to Campylobacter jejuni Colonization Is Associated with an Intestinal Immunogene Expression Signature Identified by mRNA Sequencing

peer-reviewedThis research was funded by the The Irish Department of Agriculture and Food’s Food Institutional Research Measure (http://www.agriculture.gov.ie/ research/foodinstitutionalresearchmeasurefirm) – Grant No: 06_RDD_486.Campylobacter jejuni is the most common cause of human bacterial gastroenteritis and is associated with several post-infectious manifestations, including onset of the autoimmune neuropathy Guillain-Barré syndrome, causing significant morbidity and mortality. Poorly-cooked chicken meat is the most frequent source of infection as C. jejuni colonizes the avian intestine in a commensal relationship. However, not all chickens are equally colonized and resistance seems to be genetically determined. We hypothesize that differences in immune response may contribute to variation in colonization levels between susceptible and resistant birds. Using high-throughput sequencing in an avian infection model, we investigate gene expression associated with resistance or susceptibility to colonization of the gastrointestinal tract with C. jejuni and find that gut related immune mechanisms are critical for regulating colonization. Amongst a single population of 300 4-week old chickens, there was clear segregation in levels of C. jejuni colonization 48 hours post-exposure. RNAseq analysis of caecal tissue from 14 C. jejuni-susceptible and 14 C. jejuni-resistant birds generated over 363 million short mRNA sequences which were investigated to identify 219 differentially expressed genes. Significantly higher expression of genes involved in the innate immune response, cytokine signaling, B cell and T cell activation and immunoglobulin production, as well as the renin-angiotensin system was observed in resistant birds, suggesting an early active immune response to C. jejuni. Lower expression of these genes in colonized birds suggests suppression or inhibition of a clearing immune response thus facilitating commensal colonization and generating vectors for zoonotic transmission. This study describes biological processes regulating C. jejuni colonization of the avian intestine and gives insight into the differential immune mechanisms incited in response to commensal bacteria in general within vertebrate populations. The results reported here illustrate how an exaggerated immune response may be elicited in a subset of the population, which alters host-microbe interactions and inhibits the commensal state, therefore having wider relevance with regard to inflammatory and autoimmune disease

Characterization of a Novel Clade of Transporters in Phytophthora

The oomycete Phytophthora parasitica has a worldwide distribution and is an economically important pathogen of more than 100 species4. RNA-seq analysis showed that one gene, PPTG_16698 has the 5th highest level of expression of all transport proteins in the zoospore stage, and is highly conserved throughout Phytophthora species. This project attempts to characterize the important biological role that PPTG_16698 plays in P. parasitica and other oomycetes. Three strategies have been implemented to accomplish this goal: growth analysis by heterologous expression in yeast, metabolite analysis in yeast, and construction of a GFP fusion protein to enable localization of the gene in oomycete hyphae by confocal microscopy. Confocal microscopy is expected to confirm the vacuolar localization of this gene. If this gene is localized to a vacuolar membrane, then heterologous expression in yeast should result in differential accumulation of metabolites mobilized by this transporter. In preliminary growth assays, expression of this gene did not inhibit the growth of yeast. Therefore, expression of the gene does not result in sequestering of a growth-limiting metabolite. To determine whether expression of the transporter results in the accumulation of polyamines, which are organic compounds necessary for growth in eukaryotes, polyamine levels will be measured by dansylation of amines and separation by HPLC. Other metabolites will be assayed by liquid chromatography mass spectroscopy analysis

Randomized phase II study investigating pazopanib versus weekly paclitaxel in relapsed or progressive urothelial cancer

Purpose: Two previous single-arm trials have drawn conflicting conclusions regarding the activity of pazopanib in urothelial cancers after failure of platinum-based chemotherapy. Patients and Methods: This randomized (1:1) open-label phase II trial compared the efficacy of pazopanib 800 mg orally with paclitaxel (80 mg/m2 days 1, 8, and 15 every 28 days) in the second-line setting. The primary end point was overall survival (OS). Results: Between August 2012 and October 2014, 131 patients, out of 140 planned, were randomly assigned. The study was terminated early on the recommendation of the independent data monitoring committee because of futility. Final analysis after the preplanned number of deaths (n = 110) occurred after a median follow-up of 18 months. One hundred fifteen deaths had occurred at the final data extract presented here. Median OS was 8.0 months for paclitaxel (80% CI, 6.9 to 9.7 months) and 4.7 months for pazopanib (80% CI, 4.2 to 6.4 months). The hazard ratio (HR) adjusted for baseline stratification factors was 1.28 (80% CI, 0.99 to 1.67; one-sided P = .89). Median progression-free survival was 4.1 months for paclitaxel (80% CI, 3.0 to 5.6 months) and 3.1 months for pazopanib (80% CI, 2.7 to 4.6 months; HR, 1.09; 80% CI, 0.85 to 1.40; one-sided P = .67). Discontinuations for toxicity occurred in 7.8% and 23.1% for paclitaxel and pazopanib, respectively. Conclusion: Pazopanib did not have greater efficacy than paclitaxel in the second-line treatment of urothelial cancers. There was a trend toward superior OS for paclitaxel

The European road accident observatory

The European road accident observator