Probable association of T Tauri stars with the L1014 dense core

Using the Wide Field Grism Spectrograph 2 (WFGS2), we have carried out slit-less spectroscopy, g'r'i' photometry, and slit spectroscopy on the L1014 dense core. We detected three Halpha emission line stars. We interpret one as weak-line T Tauri star (WTTS) and the others as classical T Tauri stars (CTTS). Since their g'-i' colors and/or classified spectral types are consistent with those of T Tauri stars and two of them show less extinction than the cloud, these three stars are likely to be T Tauri stars associated with L1014. Adopting an age range for T Tauri stars, 1-10 Myr, the color-magnitude diagram suggests a distance of ~400-900 pc, rather than the previously assumed distance, 200 pc. This could strongly affect on the mass estimate of L1014-IRS, which is thought to be either a very young protostar or proto-brown dwarf.Comment: 5 pages, 5 figures, to be published in Vol.58, No.5, October 25, 200

EEG alpha power and laterality during dreaming in N-REM and REM sleep

In the present study，electroencephalic (EEG) alpha power，alpha frequency and their individual laterality were studied. The dream data used in this study were collected during N-REM and REM sleeps for 3 or 4 nights. In N-REM sleep，the alpha power spectrum tend to show higher value in dreaming than in non dreaming. On the other hand，the decrement of the alpha power in REM sleep was rather apparent during dreaming. The correlation between the alpha power and the mean alpha frequency was significantly positive，while as ignificantly negative correlation was seen in N-REM sleep. Finally，we discussed the relation between the cortical activation and the alpha activities in dreaming on the basis of the results we obtained

Two distinct prions in fatal familial insomnia and its sporadic form

Abstract Fatal familial insomnia is a genetic prion disease, which is associated with the aspartic acid to asparagine substitution at codon 178 of the prion protein gene. Although the hallmark pathological feature is thalamic and olivary degeneration, there is a patient with an atypical fatal familial insomnia without the hallmark feature. The cause of the pathological variability is unclear. We analysed a Japanese fatal familial insomnia kindred and compared one atypical clinicopathological fatal familial insomnia phenotype case and typical fatal familial insomnia phenotype cases with transmission studies using multiple lines of knock-in mice and with protein misfolding cyclic amplification. We also analysed the transmissibility and the amplification properties of sporadic fatal insomnia. Transmission studies revealed that the typical fatal familial insomnia with thalamic and olivary degeneration showed successful transmission only using knock-in mice expressing human–mouse chimeric prion protein gene. The atypical fatal familial insomnia with spongiform changes showed successful transmission only using knock-in mice expressing bank vole prion protein gene. Two sporadic fatal insomnia cases with thalamic and olivary degeneration showed the same transmissibility as the typical fatal familial insomnia phenotype. Interestingly, one sporadic fatal insomnia case with thalamic/olivary degeneration and spongiform changes showed transmissibility of both the typical and atypical fatal familial insomnia phenotypes. Protein misfolding cyclic amplification could amplify both typical fatal familial insomnia cases and sporadic fatal insomnia cases but not the atypical fatal familial insomnia phenotype or other sporadic Creutzfeldt–Jakob disease subtypes. In addition to clinical findings and neuropathological features, the transmission properties and the amplification properties were different between the typical and atypical fatal familial insomnia phenotypes. It is suggested that two distinct prions were associated with the diversity in the fatal familial insomnia phenotype, and these two prions could also be detected in sporadic fatal insomnia

Development of 1 MJ Conduction-Cooled LTS Pulse Coil for UPS-SMES

A 1 MW, 1 s UPS-SMES is being developed for a protection from a momentary voltage drop and an instant power failure. As a key technology of the UPS-SMES, we developed a prototype LTS pulse coil with a stored energy of 100 kJ and conducted cooling and excitation tests in 2005. The operation test of the prototype UPS-SMES using this 100 kJ coil with power converters have been performed in 2006. A 1 MJ coil was designed before the fabrication of the 100 kJ prototype coil. The superconductor, the electric insulation technique, the winding method, and the cooling structure used for the 100 kJ coil were based upon the 1 MJ coil design. The successful performance test results of the prototype 100 kJ coil validated the design concept and fabrication technique of the 1 MJ coil. According to the achievement of the prototype 100 kJ UPS-SMES, the 1 MJ conduction-cooled LTS pulse coil has been fabricated successfully. The successful experimental results of the 100 kJ prototype coil with power converters and the fabrication procedure of the 1 MJ full size coil are described

Evaluation of Source Generators in Partial Seizures : Availability of Neuroradiological Images Superimposed on the Dipole Tracing

To clarify the anatomical localization of epileptic foci in childhood partial seizures, the source generators of interictal focal spikes were analyzed using a dipole tracing method and the effectiveness of a composite image diagnostic technique using neuroradiological images obtained by magnetic resonance imaging and single photon emission computed tomography was evaluated. The localization of electric source generators approximately reflects the abnormal lesions on neuroradiological images. However, the source generators tended to be located in the deep and/or peripheral parts of the abnormal regions. Even though systemic shifts in the position of optimal dipoles from their true positions is a limitation that must be taken into consideration, composite image diagnostic technique may be useful for evaluating the epileptogenic regions in partial seizures, and particularly, in children where noninvasive methods are preferable

¿De vuelta a Los ocho años? A propósito de La guerra de Figueres, de Guillermo Villegas Hoffmaister

El libro de Guillermo Villegas Hoffmeister, “La guerra de Figueres”, tiene sin duda el subtítulo exacto Crónica de ocho años, porque eso es lo que es. Basado en alguna documentación escrita, en sus propios recuerdos personales y en una revisión de los periodos de la década de 1940, la obra se presenta ante todo como un producto de entrevistas realizadas por el autor a distintos personajes de la época. El resultado final es una narrativa descriptiva y parcializada, que combina alguna información interesante con datos sin importancia y algunas notables inexactitudes históricas: por ejemplo, el autor afirma que ¡el partido republicano nacional fue fundado por Maximiliano Fernández en 1909!, y en lo que parece ser producto de un lamentable descuido de él y de la EUNED, fecha la batalla de tejar el 13 de marzo en vez del 13 de abril de 1948…UCR::Vicerrectoría de Investigación::Unidades de Investigación::Artes y Letras::Centro de Investigación en Identidad y Cultura Latinoamérica (CIICLA

PACAP centrally mediates emotional stress-induced corticosterone responses in mice

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide widely distributed in the nervous system. Recently, PACAP was shown to be involved in restraint stress-induced corticosterone release and concomitant expression of the genes involved in hypothalamic–pituitary–adrenal (HPA) axis activation. Therefore, in this study, we have addressed the types of stressors and the levels of the HPA axis in which PACAP signaling is involved using mice lacking PACAP (PACAP−/−). Among four different types of stressors, open-field exposure, cold exposure, ether inhalation, and restraint, the corticosterone response to open-field exposure and restraint, which are categorized as emotional stressors, but not the other two, was markedly attenuated in PACAP−/− mice. Peripheral administration of corticotropin releasing factor (CRF) or adrenocorticotropic hormone induced corticosterone increase similarly in PACAP and wild-type mice. In addition, the restraint stress-induced c-Fos expression was significantly decreased in the paraventricular nucleus (PVN) and medial amygdala (MeA), but not the medial prefrontal cortex, in PACAP−/− mice. In the PVN of PACAP−/− mice, the stress-induced c-Fos expression was blunted in the CRF neurons. These results suggest that PACAP is critically involved in activation of the MeA and PVN CRF neurons to centrally regulate the HPA axis response to emotional stressors

Immunochemical Approach for Monitoring of Structural Transition of ApoA-I upon HDL Formation Using Novel Monoclonal Antibodies

Apolipoprotein A-I (apoA-I) undergoes a large conformational reorganization during remodeling of high-density lipoprotein (HDL) particles. To detect structural transition of apoA-I upon HDL formation, we developed novel monoclonal antibodies (mAbs). Splenocytes from BALB/c mice immunized with a recombinant human apoA-I, with or without conjugation with keyhole limpet hemocyanin, were fused with P3/NS1/1-Ag4-1 myeloma cells. After the HAT-selection and cloning, we established nine hybridoma clones secreting anti-apoA-I mAbs in which four mAbs recognize epitopes on the N-terminal half of apoA-I while the other five mAbs recognize the central region. ELISA and bio-layer interferometry measurements demonstrated that mAbs whose epitopes are within residues 1–43 or 44–65 obviously discriminate discoidal and spherical reconstituted HDL particles despite their great reactivities to lipid-free apoA-I and plasma HDL, suggesting the possibility of these mAbs to detect structural transition of apoA-I on HDL. Importantly, a helix-disrupting mutation of W50R into residues 44–65 restored the immunoreactivity of mAbs whose epitope being within residues 44–65 against reconstituted HDL particles, indicating that these mAbs specifically recognize the epitope region in a random coil state. These results encourage us to develop mAbs targeting epitopes in the N-terminal residues of apoA-I as useful probes for monitoring formation and remodeling of HDL particles

Технологічні рішення і технічні засоби підвищення екологічної безпеки проведення бурових робіт

Рассмотрено влияние проведения буровых работ на окружающую среду. Приведены рекомендации для повышения экологической безопасности проведения буровых работ. Описаны технологические решения и технические средства уменьшения неблагоприятного влияния бурения нефтегазовых скважин на окружающую среду. В комплексе решены и эффективно внедрены на практике вопросы экологической безопасности проведения буровых работ, утилизации, размещения и захоронения отходов бурения. Дказано, что безамбарное бурение на месторождениях возможно при наличии шламонакопителя и нагнетательной скважины.The environmental impacts of drilling works are considered. A range of recommendations for environmental safety enhancement of drilling works is given. Technological solutions and technical means to reduce adverse environmental impacts of oil and gas wells drilling are described. The issues of drilling works environmental safety, utilization, disposal and burial of waste are jointly solved and successfully implemented. The pit less drilling is proved to be possible for any field under the condition of slurry pond and injection well use