AIP and MEN1 mutations and AIP immunohistochemistry in pituitary adenomas in a tertiary referral center.

Background: Pituitary adenomas have a high disease burden due to tumor growth/ invasion and disordered hormonal secretion. Germline mutations in genes such as MEN1 and AIP are associated with early onset of aggressive pituitary adenomas that can be resistant to medical therapy. Aims: We performed a retrospective screening study using published risk criteria to assess the frequency of AIP and MEN1 mutations in pituitary adenoma patients in a tertiary referral center. Methods: Pituitary adenoma patients with pediatric/adolescent onset, macroadenomas occurring ≤30 years of age, familial isolated pituitary adenoma (FIPA) kindreds and acromegaly or prolactinoma cases that were uncontrolled by medical therapy were studied genetically. We also assessed whether immunohistochemical staining for AIP (AIP-IHC) in somatotropinomas was associated with somatostatin analogs (SSA) response. Results: Fifty-five patients met the study criteria and underwent genetic screening for AIP/MEN1 mutations. No mutations were identified and large deletions/duplications were ruled out using MLPA. In a cohort of sporadic somatotropinomas, low AIP-IHC tumors were significantly larger (P = 0.002) and were more frequently sparsely granulated (P = 0.046) than high AIP-IHC tumors. No significant relationship between AIP-IHC and SSA responses was seen. Conclusions: Germline mutations in AIP/MEN1 in pituitary adenoma patients are rare and the use of general risk criteria did not identify cases in a large tertiary-referral setting. In acromegaly, low AIP-IHC was related to larger tumor size and more frequent sparsely granulated subtype but no relationship with SSA responsiveness was seen. The genetics of pituitary adenomas remains largely unexplained and AIP screening criteria could be significantly refined to focus on large, aggressive tumors in young patients

Relative localization for aerial manipulation with PL-SLAM

The final publication is available at link.springer.comThis chapter explains a precise SLAM technique, PL-SLAM, that allows to simultaneously process points and lines and tackle situations where point-only based methods are prone to fail, like poorly textured scenes or motion blurred images where feature points are vanished out. The method is remarkably robust against image noise, and that it outperforms state-of-the-art methods for point based contour alignment. The method can run in real-time and in a low cost hardware.Peer ReviewedPostprint (author's final draft

Technological properties of maize tortillas produced by microwave nixtamalization with variable alkalinity

This research was conducted to determine the quality, physicochemical, textural, compositional, nutritional, viscoamylographic and sensory properties of maize tortillas produced with a Modified tortilla-making process (MTMP) of variable alkalinity (0.125, 0.25 and 0.5% Ca(OH)2 w/w) and compared to the commercial brand MASECA ®. In general, tortillas from MTMP showed higher pH, total color difference (ΔE), tensile strength/cutting force, protein, lipids, crude fibre, lysine, tryptophan, in vitro protein digestibility and lower Hunter L value, loss of weight during cooking and moisture content than MASECA® tortillas. No significant differences were found in the sensory analysis of 22 descriptors of tortillas made from MASECA® and MTMP with Ca(OH)2 concentrations of 0.125 and 0.25% (w/w). However, panelist identified principal effects on changes in four attributes (aroma, appearance, flavor, and after taste flavor) and seven descriptors in tortillas from MTMP prepared with the maximum lime concentration (0.5% w/w). Microwave nixtamalization produce tortillas with acceptable physicochemical, textural, quality, compositional/nutritional and pasting properties.Key words: Maize, modified nixtamalization, tortillas, technological properties

Stokes theory of thin-film rupture

The structure of the flow induced by the van der Waals destabilization of a non-wetting liquid film placed on a solid substrate is unraveled by means of theory and numerical simulations of the Stokes equations. Our analysis reveals that lubrication theory, which yields $h_{\text{min}} \propto \tau^{1/5}$ where $h_{\text{min}}$ is the minimum film thickness and $\tau$ is the time until breakup, cannot be used to describe the local flow close to rupture. Instead, the slender lubrication solution is shown to experience a crossover to a universal self-similar solution of the Stokes equations that yields $h_{\text{min}} \propto \tau^{1/3}$, with an opening angle of $37^{\circ}$ off the solid.Comment: 12 pages, 7 figure

Calidad en el deporte de élite. Análisis de fortalezas y debilidades psicológicas en jugadores de balonmano

Determinar el perl psicológico a través de la baremación por modalidades deportivas, en este caso jugadores de balonmano, permite concretar mejor las habilidades psicológicas de cada deporte, ya que cada uno demanda unas diferentes. Por lo que el objeto de estudio es conocer la inuencia de las habilidades psicológicas en la ejecución deportiva en juga- dores de balonmano categoría élite y determinar los puntos fuertes/débiles psicológicos. Aplicando el Inventario Psicológico de Ejecución Deportiva en una muestra (n=484) con una edad que osciló entre los 12-16 años con un promedio de 13,8 años (dt=1,0) de los cuales el 47,5% eran chicos y el 52,5 chicas. Se realiza un análisis descriptivo para variables cualitativas, frecuencias absolutas y relativas, además de valores mínimos, máximos, me- dia y desviación típica. Se utiliza el coeciente de relación de Pearson para conocer las correlaciones entre variables. Para determinar las correlaciones del Inventario según genero, edad y años de práctica, se hizo la prueba MANOVA. Los resultados destacan que los jugadores de categoría élite de balonmano del estudio tienen un alto control en: afrontamiento positi- vo, control viso-imaginativo y control actitudinal, mientras que es bajo su control atencional.

Associations between macronutrient intake and serum lipid profile depend on body fat in European adolescents: The Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study

© 2014 The Authors. The present study aimed to investigate the relationships between macronutrient intake and serum lipid profile in adolescents from eight European cities participating in the HELENA (Healthy Lifestyle in Europe by Nutrition in Adolescence) cross-sectional study (2006-7), and to assess the role of body fat-related variables in these associations. Weight, height, waist circumference, skinfold thicknesses, total cholesterol, HDL-cholesterol (HDL-C), LDL-cholesterol, TAG, apoB and apoA1 were measured in 454 adolescents (44 % boys) aged 12·5-17·5 years. Macronutrient intake (g/4180 kJ per d (1000 kcal per d)) was assessed using two non-consecutive 24 h dietary recalls. Associations were evaluated by multi-level analysis and adjusted for sex, age, maternal education, centre, sum of four skinfolds, moderate-to-vigorous physical activity, sedentary behaviours and diet quality index for adolescents. Carbohydrate intake was inversely associated with HDL-C (β =-0·189, P< 0·001). An inverse association was found between fat intake and TAG (β =-0·319, P< 0·001). Associations between macronutrient intake and serum lipids varied according to adiposity levels, i.e. an inverse association between carbohydrate intake and HDL-C was only observed in those adolescents with a higher waist:height ratio. As serum lipids and excess body fat are the major markers of CVD, these findings should be considered when developing strategies to prevent the risk of CVD among adolescents.TheHELENA study was financially supported by the European Community Sixth RTD Framework Programme (contract FOOD-CT-2005-007034).Peer Reviewe