Role of Sex Peptide in Drosophila Males

Drosophila male sex peptide ACP70A is a small peptide mainly produced in the accessory glands. It elicits a high number of post-mating responses in mated females; yet its function in male physiology is not well known. Here, we explore its role in male sex behavior and pheromone biosynthesis, using males either mutant or RNAi knocked-down for Acp70A. Courtship was severely affected in both Acp70A mutants and Acp70A knocked-down males, with only 2% of the males succeeding copulation. Cuticular hydrocarbon amounts were moderately affected with 25% decrease in sp0 mutant (without Acp70A expression) and 10–22% increase in flies overexpressing Acp70A. Acp70A knock-down either ubiquitously or in the testes surprisingly resulted in an overproduction of hydrocarbons, whose amounts were double of the controls. We tested eight putative “off-target” genes but none of these led to an increase in hydrocarbon amounts. These results show that male courtship behavior is largely dependent on the presence of Acp70A and independent of cuticular hydrocarbons. The presence of potential “off-target” genes explaining the hydrocarbon phenotype is discussed

Regionalizing rainfall at very high resolution over la Réunion island using a regional climate model.

23 pagesInternational audienceRegional climate models (RCMs) should be evaluated with respect to their ability to downscale large-scale climate information to the local scales, which are sometimes strongly modulated by surface conditions. This is the case for La Reunion (southwest Indian Ocean) because of its island context and its complex topography. Large-scale atmospheric configurations such as tropical cyclones (TCs) may have an amplifying effect on local rainfall patterns that only a very high-resolution RCM, forced by the large scales and resolving finescale processes, may simulate properly. This paper documents the capability of the Weather Research and Forecasting Model (WRF) RCM to regionalize rainfall variability at very high resolution (680 m) over La Reunion island for daily to seasonal time scales and year-to-year differences. Two contrasted wet seasons (November-April) are selected: 2000-01 (abnormally dry) and 2004-05 (abnormally wet). WRF rainfall is compared to a dense network of rain gauge records interpolated onto the WRF grid through the regression-kriging (RK) technique. RK avoids the point-to-grid comparison issue, but produces imperfect estimates due to sampling, so its quality also needs to be tested. Seasonal rainfall amounts and contrasts produced by WRF are fairly realistic. At intraseasonal and daily time scales, differences to RK are more sizable. These differences are not easy to interpret in sectors where the rain gauge network is less dense and the quality of RK more uncertain, as over the eastern slopes of Piton de la Fournaise volcano where WRF seems to simulate more realistic rainfall than RK. Finally, the heavy rainfall associated with TC Ando on 6 January 2001, is documented. WRF shows weak disagreements with RK, indicating its capability to regionalize rainfall during extreme events

Laparoscopic versus open sigmoid resection for diverticulitis: long-term results of a prospective, randomized trial

Background: Elective laparoscopic sigmoid resection for diverticulitis has proven short-term benefits, but little data are available from prospective randomized trials regarding long-term outcome, quality of life, and functional results. Methods: Of 113 patients randomized to undergo laparoscopic (LAP) versus open (OP) sigmoid resection for diverticulitis, 105 (93%, LAP=54, OP=51) patients were examined and answered the Gastrointestinal Quality of Life Index (GIQLI) questionnaire, with a median follow-up of 30 (range, 9-63) months after surgery. Results: Incisional hernias were detected in five (9.8%) patients in the OP group versus seven (12.9%) in the LAP group, P=0.84). Overall satisfaction with the operation on a scale of 0 (very poor) to 10 (excellent) was 9 (range, 2-10) in the OP group versus 9 (range, 2-10) in the LAP group (P=0.78). Median GIQLI score was 115 (range, 57-144) in the OP group versus 110 (range, 61-134) in the LAP group (P=0.17). Overall satisfaction with the cosmetic aspect of the scar on a scale of 0 (very poor) to 10 (excellent) was 8 (range, 1-10) in the OP group versus 9 (range, 0-10) in the LAP group (P=0.01). Finally, median hospital cost (including reoperations for hernias) was 11,606 (5,230-147,982) CHF in the LAP group versus 12,138 (6,098-39,786) CHF in the OP group (P=0.47). Conclusions: Both open and laparoscopic approaches for sigmoid resection achieve good long-term results in terms of gastrointestinal function, quality of life, and patients' satisfaction. Significant long-term benefits of laparoscopic surgery are restricted to cosmetic (ClinicalTrials.gov protocol #NCT00453830

The duration of postoperative ileus after elective colectomy is correlated to surgical specialization

Aim: Postoperative ileus is an important factor of complications following gastrointestinal procedures. Its pathophysiology and the parameters, which may impact on its duration, remain unclear. The aim of this study was to measure the role of various clinical determinants on restoration of intestinal function after elective colorectal surgery. Methods: From July 2002 to September 2003, all patients who underwent laparotomy for colectomy (laparoscopic resections excluded) with either an ileotransverse, colocolic, or high colorectal anastomosis were entered in this prospective study. The intervals in hours between the end of the surgical procedure and passing of flatus (PG) and passing of stool (PS) were recorded by an independent investigator. PG and PS were eventually correlated with the following parameters: type of colectomy, early removal of nasogastric tube (NGT), mechanical bowel preparation (MBP), type of underlying disease, systemic administration of opiates, and surgical training (colorectal fellowship or other). Results: One hundred twenty-four patients were entered in this study. Four patients (3.2%) developed septic complications (3 anastomotic leaks and 1 intraabdominal abscess) and were excluded from the analysis. Median age in this population was 68 (range 30-95) years. Mean duration of postoperative ileus was 70±28h (PG) and 99±34h (PS). The type of colectomy, underlying disease, MBP, and early NGT removal failed, in univariate analysis, to correlate with the duration of postoperative ileus. By contrast, time intervals PG and PS were statistically shorter in the group of patients treated by a colorectal surgeon [56±23 vs 74±28h (PG); 82±26 vs 103±35h (PS), p=0.004], as well as in patients who received systemic opiates for less than 2days [64±27 vs 75±28h (PG), p=0.04; 88±32 vs 108±33h (PS), p=001]. Conclusion: Restoration of normal intestinal function after elective open colectomy takes 3 (PG) to 4 (PS) days. In our series, specialized training in colorectal surgery has a positive impact on the duration of postoperative ileus. Surgical specialization should be considered an important parameter in future clinical trials aiming to minimize postoperative ileu

Results of emergency Hartmann's operation for obstructive or perforated left-sided colorectal cancer

<p>Abstract</p> <p>Background</p> <p>Up to 15% of colorectal cancer (CRC) patients present with obstructive or perforated tumours, and require emergency surgery. The Hartmann's procedure (HP) provides the opportunity to achieve a potentially curative (R0) resection, while minimizing surgical trauma in poor-risk patients. The aim of this study was to assess the surgical (operative mortality), and oncological (long-term survival after curative resection) results of emergency HP for obstructive or perforated left-sided CRC.</p> <p>Methods</p> <p>A retrospective review of 50 patients who underwent emergency HP for perforated/obstructive CRC in our institution between 1995 and 2006.</p> <p>Results</p> <p>Median age of patients was 75 (range 22–95) years and the indications for HP were obstruction (32) and perforation (18 patients). Operative mortality and morbidity were 8% and 26% respectively. 35 patients (70%) were operated with a curative intent; in this group, overall 1-, 3- and 5-year survival rates were 80%, 54% and 40%. In univariate analysis, the presence of lymph node metastases was associated with poor 5-year survival (62% [Stage II] vs. 27% [Stage III], log-rank test, p = 0.02). Eleven patients (22%) had their operation reversed with a median delay of 225 (range 94–390) days. In this subgroup, two patients died from distant metastases, but there were no instances of loco-regional recurrence.</p> <p>Conclusion</p> <p>Hartmann's operation remains a good option to palliate symptoms in 30% of patients with left-sided CRC who are not candidates to a curative resection. For those who have a curative resection, the oncological outcome is acceptable, especially stage II patients, who appear to benefit the most from this surgical strategy.</p

Incidence, consequences, and risk factors for anastomotic dehiscence after colorectal surgery: a prospective monocentric study

Background: Anastomotic dehiscence is the most severe surgical complication after large bowel resection. This study was designed to assess the incidence, to observe the consequences, and to identify the risk factors associated with anastomotic leakage after colorectal surgery. Materials and methods: All procedures involving anastomoses of the colon or the rectum, which were performed between November 2002 and February 2006 in a single institution, were prospectively entered into a computerized database. Results: One thousand eighteen colorectal resections and 811 anastomoses were performed over this 40-month period. The most frequent procedures were sigmoid (276) and right colectomies (217). The overall anastomotic leak rate was 3.8%. The mortality rate associated with anastomotic leak was 12.9%. In univariate analysis, the following parameters were associated with an increased risk for anastomotic dehiscence: (1) ASA score ≥ 3 (p = 0.004), (2) prolonged (>3h) operative time (p = 0.02), (3) rectal location of the disease (p  25 (p = 0.04). In multivariate analysis, ASA score ≥ 3 (OR = 2.5; 95% CI 1.5-4.3, p  3h [OR = 3.0; 95% CI 1.1-8.0, p = 0.02), and rectal location of the disease (OR = 3.75; 95% CI 1.5-9.0 (vs left colon), p = 0.003; OR = 7.69; 95% CI 2.2-27.3 (vs right colon), p = 0.001] were factors significantly associated with a higher risk of anastomotic dehiscence. Conclusions: Three risk factors for anastomotic leak have been identified, one is patient-related (ASA score), one is disease-related (rectal location), the third being surgery-related (prolonged operative time). These factors should be considered in perioperative decision-making regarding defunctioning stoma formatio

Superior antigen-specific CD4+ T-cell response with AS03-adjuvantation of a trivalent influenza vaccine in a randomised trial of adults aged 65 and older

BACKGROUND: The effectiveness of trivalent influenza vaccines may be reduced in older versus younger adults because of age-related immunosenescence. The use of an adjuvant in such a vaccine is one strategy that may combat immunosenescence, potentially by bolstering T-cell mediated responses. METHODS: This observer-blind study, conducted in the United States (US) and Spain during the 2008-2009 influenza season, evaluated the effect of Adjuvant System AS03 on specific T-cell responses to a seasonal trivalent influenza vaccine (TIV) in >/=65 year-old adults.Medically-stable adults aged >/=65 years were randomly allocated to receive a single dose of AS03-adjuvanted TIV (TIV/AS03) or TIV. Healthy adults aged 18-40 years received only TIV. Blood samples were collected on Day 0, Day 21, Day 42 and Day 180. Influenza-specific CD4+ T cells, defined by the induction of the immune markers CD40L, IL-2, IFN-gamma, or TNF-alpha, were measured in ex vivo cultures of antigen-stimulated peripheral blood mononuclear cells. RESULTS: A total of 192 adults were vaccinated: sixty nine and seventy three >/=65 year olds received TIV/AS03 and TIV, respectively; and fifty 18 - 40 year olds received TIV. In the >/=65 year-old group on Day 21, the frequency of CD4+ T cells specific to the three vaccine strains was superior in the TIV/AS03 recipients to the frequency in TIV (p /=65 year-old recipients of TIV/AS03 than in the 18 - 40 year old recipients of TIV on Days 21 (p = 0.006) and 42 (p = 0.011). CONCLUSION: This positive effect of AS03 Adjuvant System on the CD4+ T-cell response to influenza vaccine strains in older adults could confer benefit in protection against clinical influenza disease in this population. TRIAL REGISTRATION: (Clinicaltrials.gov.). NCT00765076

Impact de la troposphère sur le transport isentropique dans la stratosphère via les ondes planétaires de Rossby

PARIS-BIUSJ-Thèses (751052125) / SudocPARIS-BIUSJ-Physique recherche (751052113) / SudocSudocFranceF

Validation and comparison of incoming solar radiation satellite databases on the Atlantic coast of Central Africa

International audienceSolar radiation incident on the Earth's surface is important for the functioning of tropical forests, as it affects the availability of light and water. Due to the lack of in-situ data in tropical forest environments, satellite products and reanalyses are the only ways to estimate solar radiation on a regional scale. An intercomparison of five satellite databases including CERES-EBAF, CERES-SYN1deg, CMSAF-SARAH, CMSAF-CLARA, CAMS-JADE as well as the ERA5 reanalysis, is carried out for the Atlantic coast of Central Africa by evaluating them against two in-situ data sets: the monthly FAOCLIM2 database and original infra-daily data from meteorological stations set up within the framework of ecoclimatic projects. From this inter-comparison we show the differences between these six products and with in-situ data from monthly to daily scales. We also show that the Atlantic coast of Central Africa receives the least amount of solar radiation in all products compared to other regions of Central Africa

Validation et inter-comparaison des bases de données satellitaires d’estimation de rayonnement solaire incident sur la façade atlantique d’Afrique Centrale

International audienceLe rayonnement solaire incident à la surface de la Terre est important pour le fonctionnement des forêts tropicalescar il entre en jeu dans la disponibilité en lumière et en eau. En raison du manque de données in-situ dans les milieux forestierstropicaux, les produits satellitaires et les réanalyses sont les seuls moyens d'estimer le rayonnement solaire à l'échelle régionale.Une inter-comparaison de cinq bases de données satellitaires dont CERES-EBAF, CERES-SYN1deg, CMSAF-SARAH,CMSAF-CLARA, CAMS-JADE ainsi que les réanalyses ERA5, est effectuée pour la façade atlantique d’Afrique Centrale enles évaluant par rapport à deux ensembles de données in-situ : la base de données mensuelles FAOCLIM2 et des donnéesoriginales infra-journalières issues de stations météorologiques implantées dans le cadre de projets écoclimatiques. À partir decette inter-comparaison, nous montrons les différences existantes entre ces six produits et avec les données in-situ, aux échellesmensuelles à journalières. Nous montrons également que la façade atlantique d’Afrique Centrale reçoit le moins derayonnement solaire dans l’ensemble des produits par rapport aux autres régions d’Afrique Centrale