Diversity and antibiotic resistance of aeromonas spp. in drinking and waste water treatment plants

The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene sequences. Resistance phenotypes were determined using the disc diffusion method. Aeromonas veronii prevailed in raw surface water, Aeromonas hydrophyla in ozonated water, and Aeromonas media and Aeromonas puntacta in waste water. No aeromonads were detected in ground water, after the chlorination tank or in tap water. Resistance to ceftazidime or meropenem was detected in isolates from the drinking water treatment plant and waste water isolates were intrinsically resistant to nalidixic acid. Most of the times, quinolone resistance was associated with the gyrA mutation in serine 83. The gene qnrS, but not the genes qnrA, B, C, D or qepA, was detected in both surface and waste water isolates. The gene aac(6’)-ib-cr was detected in different waste water strains isolated in the presence of ciprofloxacin. Both quinolone resistance genes were detected only in the species A. media. This is the first study tracking antimicrobial resistance in aeromonads in drinking, tap and waste water and the importance of these bacteria as vectors of resistance in aquatic environments is discussed.info:eu-repo/semantics/acceptedVersio

Synthesis of 7β-hydroxy-8-ketone opioid derivatives with antagonist activity at mu- and delta-opioid receptors

Despite extensive years of research, the direct oxidation of the 7,8-double bond of opioids has so far received little attention and knowledge about the effects of this modification on activity at the different opioid receptors is scarce. We herein report that potassium permanganate supported on iron(II) sulfate heptahydrate can be used as a convenient oxidant in the one-step, heterogeneous conversion of Delta(7.8)-opioids to the corresponding 7 beta-hydroxy-8-ketones. Details of the reaction mechanism are given and the effects of the substituent at position 6 of several opioids on the reaction outcome is discussed. The opioid hydroxy ketones prepared are antagonists at the mu- and delta-opioid receptors. Docking simulations and detailed structure-activity analysis revealed that the presence of the 7 beta-hydroxy-8-ketone functionality in the prepared compounds can be used to gain activity towards the delta opioid receptor. The 7 beta-hydroxy-8-ketones prepared with this method can also be regarded as versatile intermediates for the synthesis of other opioids of interest. (C) 2018 Elsevier Masson SAS. All rights reserved.Peer reviewe

Desafios do impresso ao digital: questões contemporâneas de informação e conhecimento

428 p., il. ; Documento TécnicoEsta publicação, como o próprio título traduz, concentra o seu foco em temas emergentes da sociedade da informação e do conhecimento, na qual, pelo impulso das tecnologias de informação e comunicação (TICs) e da globalização, informação e conhecimento são potencializados e assumem novo papel no mundo contemporâneo. Um dos objetivos desta coletânea é propiciar, aos interessados e estudiosos de questões de informação e conhecimento, uma perspectiva abrangente, às vezes instigante e não convencional de alguns de seus aspectos.UNESC

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Riscos e emergências anestésicas em procedimentos anestésicos de cães e gatos

Ministério da Educação, Universidade Federal Rural da Amazônia e Serviço de Documentação e Informação.Os procedimentos anestésicos nas intervenções cirúrgicas realizadas em caninos e felinos envolvem variados riscos, dentre os quais destacam-se vários fatores, principalmente os relacionados ao anestesista, aos agentes anestésicos e ao estado clínico do paciente, bem como as complicações que poderão advir envolvendo isolada ou conjuntamente esses fatores. O presente trabalho objetiva abordar os principais riscos durante os estados anestésicos, bem como os procedimentos que deverão ser adotados para prevenir ou reverter os acidentes provocados pelos agentes usados.The anesthetic procedures of surgery realized in canine and feline, involve several risks, with destak to several factors, mainly the ones related to the anesthetist, to the anesthetic agents, and to the clinicai status of the patient, as well as the complications that can come through involving the factors isolated orjointly. This work has the objective of dealing with the principal risks during the anesthetic state, as well as the procedures to be adopted to prevent or restore the accidents caused by the agent used

Lifetime reproductive efficiency of BALB/c mouse pairs after an environmental modification at 3 mating ages

The aim of this study was to evaluate the effects of an environment change and the age at which mating pairs were formed on the lifetime reproductive performance of BALB/c mice. We assigned 60 monogamous pairs to a randomized design in a 2 x 3 factorial arrangement (with or without an environmental modification and with 3 mating ages: 28, 45, or 60 d). Autoclaved cardboard tubes (length, 10 cm; diameter, 4 cm) were used as the environmental modification. Data were collected from a total of 456 litters over a period of 10 mo. The mice tore the cardboard tube and used its parts both as shelters and as nesting material. The presence of a cardboard tube decreased the preweaning litter mortality rate in the first 6 reproductive cycles. Mating at 28 or 45 d of age also decreased the preweaning mortality rate in the first 6 reproductive cycles, compared with monogamous pairs formed at 60 d of age. Treatments did not affect age at first parturition, number of litters, time between litters, or litter size and weight at birth and weaning. In addition to contributing to animal wellbeing, providing a cardboard tube improved productivity by decreasing the preweaning mortality rate. BALB/c siblings should be paired for mating when no older than 28 d, to reduce preweaning mortality of the offspring.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

29th Annual GP2A Medicinal Chemistry Conference

The 29th Annual GP(2)A (Group for the Promotion of Pharmaceutical chemistry in Academia) Conference was a virtual event this year due to the COVID-19 pandemic and spanned three days from Wednesday 25 to Friday 27 August 2021. The meeting brought together an international delegation of researchers with interests in medicinal chemistry and interfacing disciplines. Abstracts of keynote lectures given by the 10 invited speakers, along with those of the 8 young researcher talks and the 50 flash presentation posters, are included in this report. Like previous editions, the conference was a real success, with high-level scientific discussions on cutting-edge advances in the fields of pharmaceutical chemistry