A Simple Method to Reconstruct Firing Rates from Dendritic Calcium Signals

Calcium imaging using fluorescent reporters is the most widely used optical approach to investigate activity in intact neuronal circuits with single-cell resolution. Calcium signals, however, are often difficult to interpret, especially if the desired output quantity is membrane voltage or instantaneous firing rates. Combining dendritic intracellular electrophysiology and multi-photon calcium imaging in vivo, we recently investigated the relationship between optical signals recorded with the fluorescent calcium indicator Oregon Green BAPTA-1 (OGB-1) and spike output in principal neurons in the locust antennal lobe. We derived from these experiments a simple, empirical and easily adaptable method requiring minimal calibration to reconstruct firing rates from calcium signals with good accuracy and 50-ms temporal resolution

Distance and Regional Effects on the Value of Wild Bee Conservation

Many wild bee species are threatened across Europe, and with them the pollination function they provide. While numerous studies have assessed the value of bees as pollinators of crops, little is known about the non-marked value of bees. Using a choice modelling experiment, we examine these non-market values in Germany by identifying citizens’ willingness to pay (WTP) for wild bee conservation initiatives in four states. Effects of distance, state and regional affiliation are scrutinised, as previous research found these to affect respondents’ choices. Random parameter logit and latent class models are used to capture preference heterogeneity. Overall, we find strong support of wild bee conservation and a clear preference for improvement relative to the status quo, particularly in natural areas and for rare or endangered species. The yearly WTP for conservation initiatives ranges from 227 to 447€ per household. Our results show distance and regional effects on WTP. Initiatives in respondents’ home states are preferred, and increasing distance to initiatives in other states result in a slightly reduced WTP. Additionally, we observe regional preferences within an eastern and a western home region. These preferences are not explainable by socio-demographic characteristics, home state or distance and probably linked to social and cultural affiliations. We conclude that for widespread support in society and effective conservation initiatives, policy proposals must address this spatial heterogeneity from distance and regional effects. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.The authors thank the anonymous reviewers for their extensive and valuable comments on this manuscript. The authors would further like to thank Julian Sagebiel from the Swedish University of Agricultural Sciences for his support in the analysis of the survey data. They thank Alice Rogowski, Bennet Bergmann, Helena Leinweber, Jan Peters, Jean Paul Moreaux, Thomas Prossliner, as well as the focus groups participants for their valuable comments on the early version of the questionnaire. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. C.M., B.D. and C.R. thank the Danish National Research Foundation for its support of the Center for Macroecology, Evolution and Climate (Grant No. DNRF96)

Can existing assessment tools be used to track equity in protected area management under Aichi Target 11?

Aichi Target 11 (AT11) includes the commitment of 194 governments to equitably manage protected areas (PAs) by 2020. Here we evaluate whether existing PA Management Effectiveness (PAME) and social and governance assessment tools can be used to determine if AT11 meets equity goals. We find that PAME assessment conditions are insufficiently inclusive of relevant actors and do not satisfactorily allow for a diversity of perspectives to be expressed and accounted for, both of which are essential for equitable PA management. Furthermore, none of the analysed PAME tools fully cover multidimensional equity and thus they are inadequate for assessing progress towards equitable management in PAs. The available social and governance PA assessment tools stipulate more inclusive and participatory conditions within their guidelines, and the IUCN Governance Guidelines comprehensively capture equity dimensions in PA management, but results are not comparable across sites. We conclude that available assessment tools do not provide a reliable way to track equity in PAs at global scale. The IUCN Governance Guidelines could be adjusted to achieve this goal, providing that the information collected is made globally comparable, while ensuring transparency, accountability and room for contestation, including by communities whose livelihoods are directly implicated. Ultimately, developing and deploying globally comparable measures to evaluate equity is problematic, as the process of gathering comparable data inevitably obscures information that is highly relevant to resolving equity issues at local scales. This challenge must be met, however, if nations are to achieve and report on their success at meeting AT11 by 2020. © 2018 Elsevier LtdWe thank Jens Friis Lund from the University of Copenhagen for his great support in the writing of this publication. We also thank the following for their assistance in the data collection and analysis: April Eassom, Lauren Coad, Kathryn Knights, Jonas Geldmann, Murielle Misrachi and Naomi Kingston from UNEP-WCMC, PA Solutions, University of Oxford and University of Copenhagen, Phil Franks, Kate Schreckenberg and Dilys Roe from IIED, Marc Hockings, Fiona Leverington from IUCN WCPA/University of Queensland. N.Z-C. and N.B. acknowledge the funding provided by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 659881 to N.Z-C. and the Danish National Research Foundation for funding for the Centre for Macroecology, Evolution and Climate; grant number DNRF96 . We thank Jens Friis Lund from the University of Copenhagen for his great support in the writing of this publication. We also thank the following for their assistance in the data collection and analysis: April Eassom, Lauren Coad, Kathryn Knights, Jonas Geldmann, Murielle Misrachi and Naomi Kingston from UNEP-WCMC, PA Solutions, University of Oxford and University of Copenhagen, Phil Franks, Kate Schreckenberg and Dilys Roe from IIED, Marc Hockings, Fiona Leverington from IUCN WCPA/University of Queensland. N.Z-C. and N.B. acknowledge the funding provided by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 659881 to N.Z-C. and the Danish National Research Foundation for funding for the Centre for Macroecology, Evolution and Climate; grant number DNRF96

Semi-kinematic mount of the FIREBALL large optics

In the context of the NASA CNES FIREBALL balloon borne experiment, we present the design of a semi-kinematic mount to hold the 1 meter class mirrors of this mission. To maintain these large optics in a reasonable mass and price budgets we choose thin ULE mirrors with a thickness over diameter ratio of 1/16. Such thin mirrors require a multi support mount to reduce self weight deflection. Classical multi support mount used for ground based telescope would not survive the level of shock observed in a balloon experiment either at parachute opening or landing. To firmly maintain these mirrors in several points without noticeably deforming them we investigated the design of a two stages semi-kinematic mount composed of 24 monopods. We present the detailed design of this innovative mirror mount, the finite element modeling with the deduced optical wavefront deformation. During the FIREBALL integration and flight campaign in July 2007 at CSBF, we confirmed the validity of the mechanical concept by obtaining an image quality well within the required specifications. Variants of this approach are potentially applicable to large thin mirrors on ground-based observatories

Membrane Potential Dynamics of CA1 Pyramidal Neurons during Hippocampal Ripples in Awake Mice

Ripples are high-frequency oscillations associated with population bursts in area CA1 of the hippocampus that play a prominent role in theories of memory consolidation. While spiking during ripples has been extensively studied, our understanding of the subthreshold behavior of hippocampal neurons during these events remains incomplete. Here, we combine in vivo whole-cell and multisite extracellular recordings to characterize the membrane potential dynamics of identified CA1 pyramidal neurons during ripples. We find that the subthreshold depolarization during ripples is uncorrelated with the net excitatory input to CA1, while the post-ripple hyperpolarization varies proportionately. This clarifies the circuit mechanism keeping most neurons silent during ripples. On a finer timescale, the phase delay between intracellular and extracellular ripple oscillations varies systematically with membrane potential. Such smoothly varying delays are inconsistent with models of intracellular ripple generation involving perisomatic inhibition alone. Instead, they suggest that ripple-frequency excitation leading inhibition shapes intracellular ripple oscillations

Patterned photostimulation via visible-wavelength photonic probes for deep brain optogenetics

Optogenetic methods developed over the past decade enable unprecedented optical activation and silencing of specific neuronal cell types. However, light scattering in neural tissue precludes illuminating areas deep within the brain via free-space optics; this has impeded employing optogenetics universally. Here, we report an approach surmounting this significant limitation. We realize implantable, ultranarrow, silicon-based photonic probes enabling the delivery of complex illumination patterns deep within brain tissue. Our approach combines methods from integrated nanophotonics and microelectromechanical systems, to yield photonic probes that are robust, scalable, and readily producible en masse. Their minute cross sections minimize tissue displacement upon probe implantation. We functionally validate one probe design in vivo with mice expressing channelrhodopsin-2. Highly local optogenetic neural activation is demonstrated by recording the induced response—both by extracellular electrical recordings in the hippocampus and by two-photon functional imaging in the cortex of mice coexpressing GCaMP6

APRIL is overexpressed in cancer: link with tumor progression

<p>Abstract</p> <p>Background</p> <p>BAFF and APRIL share two receptors – TACI and BCMA – and BAFF binds to a third receptor, BAFF-R. Increased expression of BAFF and APRIL is noted in hematological malignancies. BAFF and APRIL are essential for the survival of normal and malignant B lymphocytes, and altered expression of BAFF or APRIL or of their receptors (BCMA, TACI, or BAFF-R) have been reported in various B-cell malignancies including B-cell non-Hodgkin's lymphoma, chronic lymphocytic leukemia, Hodgkin's lymphoma, multiple myeloma, and Waldenstrom's macroglobulinemia.</p> <p>Methods</p> <p>We compared the expression of <it>BAFF, APRIL, TACI and BAFF-R </it>gene expression in 40 human tumor types – brain, epithelial, lymphoid, germ cells – to that of their normal tissue counterparts using publicly available gene expression data, including the Oncomine Cancer Microarray database.</p> <p>Results</p> <p>We found significant overexpression of <it>TACI </it>in multiple myeloma and thyroid carcinoma and an association between TACI expression and prognosis in lymphoma. Furthermore, <it>BAFF and APRIL </it>are overexpressed in many cancers and we show that <it>APRIL </it>expression is associated with tumor progression. We also found overexpression of at least one proteoglycan with heparan sulfate chains (HS), which are coreceptors for APRIL and TACI, in tumors where APRIL is either overexpressed or is a prognostic factor. APRIL could induce survival or proliferation directly through HS proteoglycans.</p> <p>Conclusion</p> <p>Taken together, these data suggest that APRIL is a potential prognostic factor for a large array of malignancies.</p

Cold season emissions dominate the Arctic tundra methane budget

Arctic terrestrial ecosystems are major global sources of methane (CH4); hence, it is important to understand the seasonal and climatic controls on CH4 emissions from these systems. Here, we report year-round CH4 emissions from Alaskan Arctic tundra eddy flux sites and regional fluxes derived from aircraft data. We find that emissions during the cold season (September to May) account for >= 50% of the annual CH4 flux, with the highest emissions from noninundated upland tundra. A major fraction of cold season emissions occur during the "zero curtain" period, when subsurface soil temperatures are poised near 0 degrees C. The zero curtain may persist longer than the growing season, and CH4 emissions are enhanced when the duration is extended by a deep thawed layer as can occur with thick snow cover. Regional scale fluxes of CH4 derived from aircraft data demonstrate the large spatial extent of late season CH4 emissions. Scaled to the circumpolar Arctic, cold season fluxes from tundra total 12 +/- 5 (95% confidence interval) Tg CH4 y(-1), similar to 25% of global emissions from extratropical wetlands, or similar to 6% of total global wetland methane emissions. The dominance of late-season emissions, sensitivity to soil environmental conditions, and importance of dry tundra are not currently simulated in most global climate models. Because Arctic warming disproportionally impacts the cold season, our results suggest that higher cold-season CH4 emissions will result from observed and predicted increases in snow thickness, active layer depth, and soil temperature, representing important positive feedbacks on climate warming.Peer reviewe

Nanophotonic Neural Probes for in vivo Light Sheet Imaging

We present implantable silicon neural probes with nanophotonic waveguide routing networks and grating emitters for light sheet imaging. Fluorescein beam profiles, fluorescent bead imaging, and fluorescence brain imaging in vivo are presented

Nanophotonic Neural Probes for in vivo Light Sheet Imaging

We present implantable silicon neural probes with nanophotonic waveguide routing networks and grating emitters for light sheet imaging. Fluorescein beam profiles, fluorescent bead imaging, and fluorescence brain imaging in vivo are presented