Macaque cardiac physiology is sensitive to the valence of passively viewed sensory stimuli.

Autonomic nervous system activity is an important component of affective experience. We demonstrate in the rhesus monkey that both the sympathetic and parasympathetic branches of the autonomic nervous system respond differentially to the affective valence of passively viewed video stimuli. We recorded cardiac impedance and an electrocardiogram while adult macaques watched a series of 300 30-second videos that varied in their affective content. We found that sympathetic activity (as measured by cardiac pre-ejection period) increased and parasympathetic activity (as measured by respiratory sinus arrhythmia) decreased as video content changes from positive to negative. These findings parallel the relationship between autonomic nervous system responsivity and valence of stimuli in humans. Given the relationship between human cardiac physiology and affective processing, these findings suggest that macaque cardiac physiology may be an index of affect in nonverbal animals

An Open System for Social Computation

Part of the power of social computation comes from using the collective intelligence of humans to tame the aggregate uncertainty of (otherwise) low veracity data obtained from human and automated sources. We have witnessed a surge in development of social computing systems but, ironically, there have been few attempts to generalise across this activity so that creation of the underlying mechanisms themselves can be made more social. We describe a method for achieving this by standardising patterns of social computation via lightweight formal specifications (we call these social artifacts) that can be connected to existing internet architectures via a single model of computation. Upon this framework we build a mechanism for extracting provenance meta-data across social computations

The impact of nonlocal response on metallo-dielectric multilayers and optical patch antennas

We analyze the impact of nonlocality on the waveguide modes of metallo-dielectric multilayers and optical patch antennas, the latter formed from metal strips closely spaced above a metallic plane. We model both the nonlocal effects associated with the conduction electrons of the metal, as well as the previously overlooked response of bound electrons. We show that the fundamental mode of a metal-dielectric-metal waveguide, sometimes called the gap-plasmon, is very sensitive to nonlocality when the insulating, dielectric layers are thinner than 5 nm. We suggest that optical patch antennas, which can easily be fabricated with controlled dielectric spacer layers and can be interrogated using far-field scattering, can enable the measurement of nonlocality in metals with good accuracy

Poly(vinyl alcohol) hydrogel coatings with tunable surface exposure of hydroxyapatite.

International audienceInsufficient bone anchoring is a major limitation of artificial substitutes for connective osteoarticular tissues. The use of coatings containing osseoconductive ceramic particles is one of the actively explored strategies to improve osseointegration and strengthen the bone-implant interface for general tissue engineering. Our hypothesis is that hydroxyapatite (HA) particles can be coated robustly on specific assemblies of PVA hydrogel fibers for the potential anchoring of ligament replacements. A simple dip-coating method is described to produce composite coatings made of microscopic hydroxyapatite (HA) particles dispersed in a poly(vinyl alcohol) (PVA) matrix. The materials are compatible with the requirements for implant Good Manufacturing Practices. They are applied to coat bundles of PVA hydrogel fibers used for the development of ligament implants. By means of optical and electronic microscopy, we show that the coating thickness and surface state can be adjusted by varying the composition of the dipping solution. Quantitative analysis based on backscattered electron microscopy show that the exposure of HA at the coating surface can be tuned from 0 to over 55% by decreasing the weight ratio of PVA over HA from 0.4 to 0.1. Abrasion experiments simulating bone-implant contact illustrate how the coating cohesion and wear resistance increase by increasing the content of PVA relative to HA. Using pullout experiments, we find that these coatings adhere well to the fiber bundles and detach by propagation of a crack inside the coating. These results provide a guide to select coated implants for anchoring artificial ligaments

What's in a name? Exploiting URIs to enrich provenance explanations in plain English

Provenance allows decision-makers to evaluate the importance of pieces of data. PROV is the standardised model of provenance for use on the web, particularly suited for situations where data is generated by systems under distributed control, such as in coalition operations. If human decision-makers are to make effective use of provenance data, they need to understand it, and this work establishes techniques for explaining PROV graphs to human users in natural English.In this paper, we demonstrate the potential role of exploiting the linguistic information that is informally encoded in the URIs used to denote provenance data resources to generate these more natural English explanations of provenance. We show how this additional linguistic information allows us to generate richer, more readable explanation texts, thus enabling better decision-making and increasing the value of preexisting provenance data.<br/

Methods to analyze SNARE-dependent vesicular fusion events that regulate autophagosome biogenesis.

Autophagy is an important catabolic pathway that preserves cellular homeostasis. The formation of autophagosomes is a complex process requiring the reorganization of membranes from different compartments. Here we describe methods to analyze SNARE-dependent vesicular fusion events involving the homotypic and heterotypic fusion of autophagosome precursor structures. These two steps are essential for the maturation of small single-membrane autophagic precursors containing ATG16L1 and mATG9 proteins into double-membrane autophagosomes. The techniques described in this review are mostly based on live cell imaging, microscopy, and biochemistry using an in vitro fusion assay, and should help researchers to study autophagosome biogenesis.We are grateful to the Wellcome Trust (Principal Research Fellowship to DCR), a Wellcome Trust/MRC Strategic award in neurodegeneration, and a Wellcome Trust Strategic Grant to Cambridge Institute for Medical Research for funding.This is the final version. It was first published by Elsevier at http://www.sciencedirect.com/science/article/pii/S104620231400365

G2MARS a collaborative approach to improve fodder quality of European sorghum germplasm

The ability of sorghum to maintain a high level of biomass production in stressful environments makes it an interesting crop for animal feed as fodder, and for biomaterials and bioenergy production. Sorghum hybrids with improved agronomical behavior and high biomass quality are being developed for the European market. However the development of such hybrids requires a lot of field testing and biomass analyses. The current marker assisted breeding strategies offer the possibilities to better understand the genetic architecture of biomass quality and accelerate the creation of improved varieties. The G2MARS design developed in the frame of the “Biomass For the Future” project is gathering the know-how of both private companies and publics institutes to deliver new genetic marker and improved germplasm for the European biomass market. Seven biparental connected populations were developed in both genetic pools (3 in the male, 3 in the female and one male-female populations). These populations involved elite commercial cultivars and high biomass quality sorghum landraces. A total of 964 hybrids from these 7 populations were characterized in 2016 and 2017 in 2 locations. Agronomical traits and biomass quality related traits were measured. A QTL detection taking advantage of the connected design was performed with MCQTL and allowed the detection of 17 and 6 QTL respectively in the male and female pools. For each QTL the parental alleles were ranked allowing to define 2 target molecular ideotypes. The crossing strategy aiming to combine the most efficiently the favorable alleles was defined using Optimas. The G2MARS project offered the opportunity to take advantage of the specific skills of both private and public partners and develop a joint breeding action do develop hybrids suitable to fit the expectations of the biomass value chains. IN addition, this design improved the understanding of the genetic architecture of biomass quality traits that was mobilized to optimize the recombination strategy. This work was supported by the Biomass For the Future project (ANR-11-BTBR-0006-BFF) funded by the French National Research Agency (ANR)

Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections.

Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific oysters.This work was partially funded through the EU project Bivalife (FP7 KBBE, contract n 266157), the Poitou Charentes Region and DPMA (Direction des p^eches maritimes et de l’aquaculture, AESTU project). David Rubinsztein is aWellcome Trust Prinicipal Research Fellow.This is the final published version. It first appeared at http://www.tandfonline.com/doi/full/10.1080/15548627.2015.1017188