Features cell cycle rat hepatocytes under the experimental hypothyroidism and hyperthyroidism

Thyroid hormones are necessary for normal development, growth and functioning of organs. The experiment investigated the changing phases of the cell cycle of liver cells in rats under simulatedhypothyroidism and hyperthyroidism. The experiment was conducted on 40 white laboratory rats with body mass 120-150g. The animals were divided into groups: №1 - 15 animals - model hypothyroidism, administration Merkazolil 10 mg / kg, №2 - 15 animals - model hyperthyroidism due tointroduction of the L-thyroxine 200 mg / kg. Drugs were administered intragastric with 1% starch slurry once a day. Group №3, control - 10 rats wereinjected only solvent. After the withdrawal of animals from the experiment by decapitation under ketaminanesthesia, part of the liver size 1x1 cm was removedfromsame location. Cytofluorometric analysis was conducted on the multifunctional research flow cytometer "PartecPAS" company Partec (Germany) SIC VNMU named after Pirogov.In experimental hypothyroidism significant increase of the number of cells in a state of apoptosis and reduced proliferation activity was observed compared to controls. In experimental hyperthyroidism growing number of nuclei of cells in a state of apoptosis and increased proliferation activity wasobserved compared to controls, this indicats activation of reparative regeneration processes

THE INFLUENCE OF MILK-CLOTTING ENZYMES ON THE FUNCTIONAL PROPERTIES OF PIZZA-CHEESES

The effect of the type and dose of milk-clotting enzymes (Chy-max® M based on recombinant camel chymosin, Fromase® TL based on Rhizomucor miehei protease) on the physicochemical, functional properties and shelf life of pizza-cheeses was studied. When using a low dose of milk-clotting enzymes (MCE) for milk coagulation (250–1100 IMCU per 100 kg of milk), cheeses were obtained with an increased moisture content (55–57%), excessive acidity (pH 4.8–4.9) and texture defects (incoherent, crumbly, with separation of free moisture). This is due to the formation of a weak curd, which releases moisture poorly during processing. The use of an increased dose of MCE makes it possible to obtain a denser curd, better releasing moisture. Cheese produced with a high dose of milk-clotting enzymes (2000–2800 IMCU per 100 kg of milk) had a lower moisture content (52–53%) and lower acidity (pH 5.0–5.1). The protein matrix is more hydrated in these cheeses, which ensures its better water holding capacity and a more homogeneous and cohesive texture. The use of an increased dose of MCE with a high total proteolytic activity (Fromase) gives undesirable consequences in the form of accelerated proteolysis of cheese mass proteins, rapid loss of functional properties of the cheese, and a decrease in the shelf life of cheese (less than 60 days). Cheese production using an increased dose of MCE with a low level of total proteolytic activity (Chy-max M) allows achieving a low level of proteolysis during cheese ripening and increasing its shelf life

Synthesis of Nanostructured Catalysts by Surfactant-Templating of Large-Pore Zeolites

Zeolites and related crystalline molecular sieves are utilised in a wide range of reactions and processes due to their regular microporous structure, strong acidity, shape selectivity and ion-exchange properties. However, their practical applications can be limited by the small size of the channels and cavities of the microporous structures, and therefore, a great deal of effort have been devoted to enhancing the transport of large-sized molecules in the host pores. Several commercially available zeolites, including faujasite (FAU), mordenite (MOR), beta (BEA), ZSM-5 (MFI) and zeolite L (LTL) have been exposed to a variety of acid and base treatments in the presence of a surfactant (cetyltrimethyl ammonium bromide, CTAB), which led to the controlled introduction of intracrystalline mesoporosity. A detailed characterisation of the obtained mesostructured zeolites has been carried out using FTIR spectroscopy, high resolution TEM, XRD, N2 adsorption, 29Si and 27Al MAS NMR. This work demonstrates a successful application of the supramolecular templating approach for generating tuneable mesoporosity in a range of zeolites possessing 12-membered ring channels, which has been applied to zeolite L for the first time, thus producing hierarchical meso-microporous materials with improved accessibility of active sites and enhanced catalytic performance in dealkylation of tri-isopropylbenzene

Diffusion doping route to plasmonic Si/SiOx nanoparticles

International audienceSemiconductor nanoparticles (SNPs) are a valuable building block for functional materials. Capabilities for engineering of electronic structure of SNPs can be further improved with development of techniques of doping by diffusion, as post-synthetic introduction of impurities does not affect the nucleation and growth of SNPs. Diffusion of dopants from an external source also potentially allows for temporal control of radial distribution of impurities. In this paper we report on the doping of Si/SiOx SNPs by annealing particles in gaseous phosphorus. The technique can provide efficient incorporation of impurities, controllable with precursor vapor pressure. HRTEM and X-ray diffraction studies confirmed that obtained particles retain their nanocrystallinity. Elemental analysis revealed doping levels up to 10%. Electrical activity of the impurity was confirmed through thermopower measurements and observation of localized surface plasmon resonance in IR spectra. The plasmonic behavior of etched particles and EDX elemental mapping suggest uniform distribution of phosphorus in the crystalline silicon cores. Impurity activation efficiencies up to 34% were achieved, which indicate high electrical activity of thermodynamically soluble phosphorus in oxide-terminated nanosilicon

Classicism as a Reversing Stage in the Development of Art

The subject of the research is classicism as a reversible stage in the development of art and artistic culture. At the end of the study, the author puts forward the following assumption: since the attitude and worldview of the same N. Poussin or Ingres, as it should be assumed was different than that of Рolyclet and Phidias, and the logic of their creative path should be different. However, classicism, fulfilling the mission of the great style, at the same time proved to be a reversible stage in the development of art.Предметом исследования является классицизм как реверсивный этап в развитии искусства и художественной культуры. В конце исследования авторы выдвигают следующее предположение: поскольку мироощущение и мировосприятие того же Н. Пуссена или Энгра, как надо полагать, было иным, нежели у Поликлета и Фидия, то и логика их творческого пути должна быть различной. Однако классицизм, выполнив миссию великого стиля, в то же время проявил себя как реверсивный этап в развитии искусства

Cardiovascular Toxicity in Copper Production Workers Exposed to Heavy Metals

Introduction. Cardiovascular diseases (CVD) are a leading cause of morbidity and mortality. The role of occupational hazards in the CVD prevalence remains to be clarified. Material and methods. Here we report the results of the study of risk factors and CVD prevalence in 590 workers at the largest copper production plants in the Sverdlovsk region, exposed to heavy metals in the workplace. The workers' health information was obtained during a regular medical examination in 2018. The lead concentration increase to 1.3-1.8 occupational exposure limits was registered in the working areas of the concentrating mill (for bunkerman) and copper smelting workshops (transporter, smelter, converter, non-ferrous metal spreader, repairman, electrician). Results. We studied the exposure indices (Pb level in blood), the response markers (reticulocyte count, erythrocytes basophilic stippling, coproporphyrin, and aminolevulinic acid in the urine), and their correlation to a working tenure. Based on this analysis, we attributed CVD risk factors and cardiovascular diseases to the occupation, in order to potentially modify some of those risk factors and ultimately inform the risk management. Hypertension occurred in 57% of the examined workers, which is higher than in the general population. We calculated relative risk, confidence intervals and attributable fraction. We developed a predictive mathematical model (stepwise logistic regression) to predict high-stage hypertension and identified the risk factors associated with its development. Conclusions. Correlation analysis revealed direct correlations between stages 2 and 3 hypertension and a working tenure over 20 years. We think it's reasonable to consider the documented CVDs as related to the toxic effects of heavy metals (lead and cadmium). © 2020 Izdatel'stvo Meditsina. All rights reserved.The work of Solodushkin was supported by Act 211 Government of the Russian Federation, contract No 02.A03.21.0006

Abdominal obesity diagnostics in clinical practice

According to the WHO, there are 2 bilUon of obese people worldwide. Obesity prevalence is increasing in developed countries, and also tends to increase in developing regions. Some researchers have demonstrated that abdominal obesity (АО), and not general obesity with increased body mass index (MBI), is associated with raised cardiovascular risk. АО is diagnosed by computed tomography and anthropometry: measurement of waist circumference, waist/hips circumference ratio, sagittal abdominal diameter (SAD). SAD is linked to coronary risk and insulin resistance; therefore, is can be used for identifying obese patients with high risk of type 2 diabetes mellitus and coronary heart disease

Self-assembly of multi-hierarchically structured spongy mesoporous silica particles and mechanism of their formation

International audienceHere we report on self-assembly of novel multi-hierarchically structured meso(nano)porous colloidal silica particles which have cylindrical pores of 4-6 nm, overall size of 10 gm and "cracks" of 50-200 nm. These cracks make particles look like micro-sponges. The particles were prepared through a modified templated sol-gel self-assembly process. The mechanism of assembly of these particles is investigated. Using encapsulated fluorescent dye, we demonstrate that the spongy particles are advantageous to facilitate dye diffusion out of particles. This multi-hierarchically geometry of particles can be used to improve the particle design for multiple applications to control drug release, rate of catalysis, filtration, utilization of particles as hosts for functional molecules (e.g., enzymes), etc. (C) 2016 Elsevier Inc. All rights reserved