Molekularni dokaz infekcija uzrokovanih hemoplazmama u pasa u južnom Iranu i njihovo razlikovanje na osnovi polimorfizma dužine restrikcijskog fragmenta.

Two hemoplasma species are known in dogs: Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp). The aim of the present study was to develop a novel restriction fragment length polymorphism (RFLP)-PCR method based on the 16S rDNA gene, using endonuclease Hind III, for detection and differentiation of canine hemoplasmas. Also, analysis of risk factors, clinical features and hematologic changes of positive cases was performed in dogs living in the Shiraz area of Iran. Blood samples were collected from anemic (packed cell volume (PCV) ≤35; n = 26) and control dogs (PCV >35; n = 27) and were examined for the presence of canine hemoplasmas, using RFLP-PCR and 16S rDNA Sanger sequencing. The presence of Mhc (4 out of 53 cases; 7.5%) and CMhp (3 out of 53 cases; 5.7%) was confirmed by RFLP analysis of the amplified 16S rDNA and sequencing. No association was found between hemoplasma infection and anemia, health status, age, breed, gender, type of housing or the presence of other dogs in this study. Only the platelet number in Mhc infected dogs was statistically higher compared to CMhp positive and hemoplasma negative dogs. The present report documents the occurrence of Mhc and CMhp in southern Iran, and these hemotropic Mycoplasma infections must be expected even in the absence of clinical symptoms or hematologic abnormalities in dogs. For the first time, it has been indicated that RFLP-PCR assay is able to successfully distinguish hemotropic Mycoplasma in dogs.U pasa su poznate dvije vrste hemoplazama: Mycoplasma haemocanis (Mhc) i Candidatus Mycoplasma haematoparvum (CMhp). Cilj je ovog istraživanja bio razviti novu metodu za dokaz i razlikovanje pasjih hemoplazama temeljenu na određivanju polimorfizma dužine restrikcijskog fragmenta (PDRF) gena 16S rDNA uporabom endonukleaze Hind III. Analizirani su i rizični čimbenici, kliničke osobitosti i hematološke promjene u inficiranih pasa na području Shiraza u Iranu. Uzorci krvi bili su prikupljeni od anemičnih (hematokrit ≤35; n = 26) i kontrolnih pasa (hematokrit >35; n = 27) te pretraženi na prisutnost pasjih hemoplazama metodom RFLPPCR i Sangerovom metodom sekvenciranja 16S rDNA. Prisutnost Mhc (4 od 53 slučaja; 7,5%) i CMhp (3 od 53 slučaja; 5,7%) bila je potvrđena analizom polimorfizma restrikcijskog fragmenta 16S rDNA i sekvenciranjem. Nije ustanovljena veza između infekcije hemoplazmama i anemije te zdravstvenog stanja, dobi, pasmine, spola, načina držanja i prisutnosti drugih pasa. Jedino je broj trombocita u pasa inficiranih vrstom Mycoplasma haemocanis bio statistički značajno veći od onih inficiranih Candidatus Mycoplasma haematoparvum i pasa negativnih na hemoplazme. Ovo izvješće potkrepljuje prisutnost Mhc i CMhp u južnom Iranu. Infekcije hemotropnim mikoplazmama mogu se očekivati i u pasa bez kliničkih znakova ili hematoloških poremećaja. Prvi put je pokazano da se metodom RFLP-PCR mogu uspješno razlikovati hemotropne mikoplazme u pasa

Petrology and geochemistry of Plio-Quaternary high-Nb basalts from Shahr-e-Babak area:Insights into post-collision magmatic processes in the Kerman Cenozoic Magmatic Arc

Post-collision Pliocene-Quaternary basaltic rocks outcrop in the Kerman Cenozoic Magmatic Arc (KCMA) to the northwest and east of Shahr-e-Babak city. These porphyritic and vesicular basaltic rocks are composed essentially of clinopyroxene, olivine, and plagioclase. These basalts display alkaline affinity and negative Ta, Zr, Rb anomaly, but slightly negative Nb anomaly, relative to elements with similar compatibility, and positive Ba, K, Sr anomaly, suggesting their magma source related to subduction-accretion with implication of subducted slab derived components to the source. In the primitive mantle and chondrite normalized diagrams, these rocks show trace elements (except depletion in Nb, Ta) and Rare Earth Element (REE) patterns similar to the Ocean Island Basalts (OIB) and share trace and major element characteristics similar to High-Nb Basalts (HNBs). Geochemical analyses for major and trace elements suggest that the Shahr-e-Babak HNBs have undergone insignificant crustal contamination and minor olivine + Fe-Ti oxide ±clinopyroxene fractional crystallization. These HNBs derived from a partial melting (~5%) of garnet-peridotite mantle wedge, which have already metasomatized by overlying sediments, fluids, and adakitic (slab-derived) melts as major metasomatic agents in post-collision setting in the KCMA. We conclude that asthenospheric upwelling arising from slab break-off followed by the roll-back of subducting Neotethys slab also triggered metasomatized peridotite mantle wedge and caused its partial melting in the subduction zone

Psychological needs of men under methadone maintenance treatment: A mixed method study

Background: The psychosocial needs of participants who are responding to the methadone maintenance treatment method can be considered an important issue in the healing process. This study has had the aim of determining the psychosocial needs of patients who were receiving methadone therapy. Methods: This was a simultaneous mixed method study. On the qualitative side of the study, using the snowball sampling method, 19 male clients who were interested in participating in the study with at least one month of methadone therapy were selected, after which in-depth, semi-structured individual and face-to-face interviews were conducted. On the quantitative side, a descriptive study was carried out; to initiate the study, 136 male clients from 12 substance abuse treatment clinics in Kermanshah City located in the west of Iran were selected by applying the convenience sampling method and analysed using the Basic Needs Satisfaction scale. The content analysis method was used to analyse qualitative data, whereas quantitative data were analysed by SPSS 18 software. Results: The qualitative results comprised 15 subthemes and 5 main themes. The main categories included the need for support, the need for tranquillity, fear of ostracism, inadequate self-esteem and a vague self-image. The quantitative results indicated dependence on methadone, together with a weak sense of self-determination and competence; these findings were consistent with the qualitative results. The main limitation of the study was disagreement by some participants over the issue of recording interviews. Conclusions: The results showed that drug-using participants need the support of their family and community during treatment to satisfy their psychosocial needs

Molekularni dokaz infekcija uzrokovanih hemoplazmama u pasa u južnom Iranu i njihovo razlikovanje na osnovi polimorfizma dužine restrikcijskog fragmenta.

Two hemoplasma species are known in dogs: Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp). The aim of the present study was to develop a novel restriction fragment length polymorphism (RFLP)-PCR method based on the 16S rDNA gene, using endonuclease Hind III, for detection and differentiation of canine hemoplasmas. Also, analysis of risk factors, clinical features and hematologic changes of positive cases was performed in dogs living in the Shiraz area of Iran. Blood samples were collected from anemic (packed cell volume (PCV) ≤35; n = 26) and control dogs (PCV >35; n = 27) and were examined for the presence of canine hemoplasmas, using RFLP-PCR and 16S rDNA Sanger sequencing. The presence of Mhc (4 out of 53 cases; 7.5%) and CMhp (3 out of 53 cases; 5.7%) was confirmed by RFLP analysis of the amplified 16S rDNA and sequencing. No association was found between hemoplasma infection and anemia, health status, age, breed, gender, type of housing or the presence of other dogs in this study. Only the platelet number in Mhc infected dogs was statistically higher compared to CMhp positive and hemoplasma negative dogs. The present report documents the occurrence of Mhc and CMhp in southern Iran, and these hemotropic Mycoplasma infections must be expected even in the absence of clinical symptoms or hematologic abnormalities in dogs. For the first time, it has been indicated that RFLP-PCR assay is able to successfully distinguish hemotropic Mycoplasma in dogs.U pasa su poznate dvije vrste hemoplazama: Mycoplasma haemocanis (Mhc) i Candidatus Mycoplasma haematoparvum (CMhp). Cilj je ovog istraživanja bio razviti novu metodu za dokaz i razlikovanje pasjih hemoplazama temeljenu na određivanju polimorfizma dužine restrikcijskog fragmenta (PDRF) gena 16S rDNA uporabom endonukleaze Hind III. Analizirani su i rizični čimbenici, kliničke osobitosti i hematološke promjene u inficiranih pasa na području Shiraza u Iranu. Uzorci krvi bili su prikupljeni od anemičnih (hematokrit ≤35; n = 26) i kontrolnih pasa (hematokrit >35; n = 27) te pretraženi na prisutnost pasjih hemoplazama metodom RFLPPCR i Sangerovom metodom sekvenciranja 16S rDNA. Prisutnost Mhc (4 od 53 slučaja; 7,5%) i CMhp (3 od 53 slučaja; 5,7%) bila je potvrđena analizom polimorfizma restrikcijskog fragmenta 16S rDNA i sekvenciranjem. Nije ustanovljena veza između infekcije hemoplazmama i anemije te zdravstvenog stanja, dobi, pasmine, spola, načina držanja i prisutnosti drugih pasa. Jedino je broj trombocita u pasa inficiranih vrstom Mycoplasma haemocanis bio statistički značajno veći od onih inficiranih Candidatus Mycoplasma haematoparvum i pasa negativnih na hemoplazme. Ovo izvješće potkrepljuje prisutnost Mhc i CMhp u južnom Iranu. Infekcije hemotropnim mikoplazmama mogu se očekivati i u pasa bez kliničkih znakova ili hematoloških poremećaja. Prvi put je pokazano da se metodom RFLP-PCR mogu uspješno razlikovati hemotropne mikoplazme u pasa

The barriers to achieve financial protection in Iranian health system: a qualitative study in a developing country

Background: The move to universal health coverage and consequently health promotion is influenced by political, socio-economic and other contextual factors in a country. Iran, as a developing country with an upper-middle national income, has developed policies to achieve universal health coverage through financial protection. This study aims to investigate barriers to develop financial protection as a requirement to achieve universal health coverage.Materials and Methods: This qualitative study was conducted using 20 in-depth interviews with experts in social welfare, health insurance and financing. The framework analysis method was used to analyze the data.Results: The results have been categorized in three major themes that were extracted from ten sub-themes. The major themes included the political, social and economic context of the country, the context and structure of healthcare system and dimensions of UHC.Conclusion: Achieving financial protection as a long-term objective should be considered as a priority among Iranian policy makers that requires an inter-sectoral collaboration with a defined in-charge body. Health policy makers in Iran should develop a more comprehensive benefits package for diseases and health conditions with catastrophic consequences. They also should develop a plan to cover the poor people.Keywords: Universal Health Coverage, Financial Protection, Catastrophic Health Expenditures, Impoverishing Health Expenditure

Petrology and geochemistry of Plio-Quaternary high-Nb basalts from Shahr-e-Babak area : insights into post-collision magmatic processes in the Kerman Cenozoic Magmatic Arc

Post-collision Pliocene-Quaternary basaltic rocks outcrop in the Kerman Cenozoic Magmatic Arc (KCMA) to the northwest and east of Shahr-e-Babak city. These porphyritic and vesicular basaltic rocks are composed essentially of clinopyroxene, olivine, and plagioclase. These basalts display alkaline affinity and negative Ta, Zr, Rb anomaly, but slightly negative Nb anomaly, relative to elements with similar compatibility, and positive Ba, K, Sr anomaly, suggesting their magma source related to subduction-accretion with implication of subducted slab derived components to the source. In the primitive mantle and chondrite normalized diagrams, these rocks show trace elements (except depletion in Nb, Ta) and Rare Earth Element (REE) patterns similar to the Ocean Island Basalts (OIB) and share trace and major element characteristics similar to High-Nb Basalts (HNBs). Geochemical analyses for major and trace elements suggest that the Shahr-e-Babak HNBs have undergone insignificant crustal contamination and minor olivine + Fe-Ti oxide ±clinopyroxene fractional crystallization. These HNBs derived from a partial melting (~5%) of garnet-peridotite mantle wedge, which have already metasomatized by overlying sediments, fluids, and adakitic (slab-derived) melts as major metasomatic agents in post-collision setting in the KCMA. We conclude that asthenospheric upwelling arising from slab break-off followed by the roll-back of subducting Neotethys slab also triggered metasomatized peridotite mantle wedge and caused its partial melting in the subduction zone

The Effects of Parental Socioeconomic Status on Children’ Physical Activity: Mediating Role of Motivation

Background: An important determinant of health and wellbeing is socioeconomic status as it can influence an individual’s exposure to several risk factors across the lifespan. Previous studies, using the self-report scales, have demonstrated that parental socioeconomic status is associated with physical activity in children. However, due to limitations of self-reporting methods, we aimed to assess the association between the parental socioeconomic statuses with accelerometer-measured physical activity among children. Motivation was included as a mediator.Method: 126 participants (45 girls, mean age of 10.92±1.89 years) wore the accelerometer for seven consecutive days. Parental socioeconomic status was measured by two items, namely, the parents’ education level and household income. The Intrinsic Motivation Scale was applied to measure motivation. Structural equation modeling was performed to analyze data.Results: 74% of our sample was at medium level of parental socioeconomic status. Accelerometer data demonstrated that children spent 74.29% of the total time in sedentary behaviors, 17.20% in light physical activity, and 8.81% in Moderate-to-Vigorous Physical Activity (MVPA). On average, the daily time spent in MVPA was 45.19 minutes, which is below the WHO guideline. In fact, 27.7% (n=35) of children fulfilled the guideline. The results showed that parental socioeconomic status had significant effects on motivation (T=4.129) and MVPA (T=5.097). Moreover, motivation had a significant effect on MVPA (T=2.679). Finally, motivation significantly mediated the association between socioeconomic status and MVPA (P<0.001).Conclusion: As demonstrated in the findings, the socioeconomic statuses of parents and physical activity have become critical concerns about children. Accordingly, in order to increase the level of MVPA among children, large scale programs should be developed by the stakeholders to increase the parents’ socioeconomic status level

Comparing the Effectiveness of Face-to-Face and Non-Face-to-Face Training on Oral and Dental Health Behaviors in Patients with Type 2 Diabetes: A Randomized Clinical Trial

Objective: Diabetes is closely related to oral and dental health. Several oral diseases and disorders are associated with diabetes. The present study was conducted to compare the effectiveness of face-to-face and non-face-to-face training of oral and dental health behaviors based on the Health Belief Model (HBM) in patients with type 2 diabetes. Materials and methods: This study was conducted among patients with type 2 diabetes referring to comprehensive health centers in Andimeshk in 2021. One hundred twenty patients with type 2 diabetes were randomly divided into two groups: a face-to-face (60 patients) training group and a non-face-to-face (60 patients) training group. For the face-to-face training group, training programs were conducted through lectures with questions and answers, group discussions, and videos in three 60-minute sessions; and for the other group through the presentation of patient education pamphlets. The results were collected in two stages before and two months after the intervention using a questionnaire. Then the data were analyzedusing a chi-squared test, t-test, and paired t-tests in SPSS Statistics 22.0. Results: A total of 120 patients participated in this study. The patients’ mean age was 51.5 ± 8.57 and 51.9 ± 10.1 years in the control and the intervention group, respectively. In both groups, 60% of the participants were female and 95% were married. The duration of diabetes in 57.5% of them was 5–10 years. After the implementation of the intervention, a significant increase was observed in the mean scores of all HBM constructs in the face-to-face training group (p &lt; 0.001). However, there was no significant change in the mean scores of the HBM constructs in the non-face-to-face training group after the intervention (p &gt; 0.05). Conclusions: This study showed that the design and implementation of an in-person training program had a positive effect on improving oral and dental health behaviors in patients with diabetes based on the HBM

Investigation of nitrate and nitrite concentration and other physicochemical parameters of drinking water sources in Saveh city during the year of 2018

Abstract   Background and Purpose:Qualitative parameters of drinking water such as concentration of nitrate, nitrite, sulfate, total soluble solids, sodium, magnesium, fluoride, total hardness and electrical conductivity can play an important role in groundwater resources and are mainly related to agriculture, waste disposal areas and sewage. The aim of this study was to determine these parameters in drinking groundwater resource of Saveh city using Geographic Information System during the year of 2018 and investigation of contaminant’s in the region’s aquifer. Materials and Methods:This research is a descriptive-analytic study. 120 samples of water from 12 drinking water wells were prepared in spring and two times in the morning and afternoon in different parts of the city of Saveh. The quality parameters of drinking water, including concentration of nitrate, nitrite, sulfate, total soluble solids, sodium, magnesium, fluoride, total hardness and electrical conductivity were entered into the GIS software and stored in a database and then processed by the information system software, color mapping was prepared and geographical maps (GIS) were mapped to qualitative status. Also, reverse interpolation was used to estimate the conditions of the whole region. Findings:The average concentration of chlorine, sulfate, electrical conductivity, total soluble solids, total hardness and sodium exceeds the permissible limits, and the non-qualitative water conditions are quite evident. Also, the amount of two magnesium and sulfate ions was above the standard 1053. It should be kept in mind that the high levels of these two ions can interfere with the digestive system. Fluoride and nitrate levels were also acceptable range in all areas. Conclusion:The highest deviation level from 1053 standard was for total soluble solids, sodium, magnesium, and sulfate. It is better to consider a comprehensive program to solve the problem, including use of nanotechnology, filtering or ion exchange