Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines

Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell susceptibility. Calbindin D28k (CbD28k) is highly expressed in Purkinje cells and has been used as a marker for normal and degenerative Purkinje cells. The goal of this study was to describe S bonariense-induced disease by ascertaining Purkinje cell-specific degenerative changes using CbD28k expression and to correlate this with apoptosis in Purkinje cells, as determined using TUNEL (transferase-mediated dUTP-biotin nick end-labeling) and ultrastructural changes. In all cases, an increase in both dose and duration of S bonariense intoxication resulted in a decrease in the number of Purkinje cells. CbD28k immunohistochemistry was an excellent marker for Purkinje cells because immunoreactivity did not change in normal or degenerative tissues. This finding suggests that excessive calcium excitatory stimulation does not induce rapid neuronal degeneration and death. As found in previous studies, TUNEL tests and electron microscopy suggest that Purkinje cell degeneration and death are not occurring via an apoptotic process. These findings suggest that S bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Facultad de Ciencias Veterinaria

Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines

Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell susceptibility. Calbindin D28k (CbD28k) is highly expressed in Purkinje cells and has been used as a marker for normal and degenerative Purkinje cells. The goal of this study was to describe S bonariense-induced disease by ascertaining Purkinje cell-specific degenerative changes using CbD28k expression and to correlate this with apoptosis in Purkinje cells, as determined using TUNEL (transferase-mediated dUTP-biotin nick end-labeling) and ultrastructural changes. In all cases, an increase in both dose and duration of S bonariense intoxication resulted in a decrease in the number of Purkinje cells. CbD28k immunohistochemistry was an excellent marker for Purkinje cells because immunoreactivity did not change in normal or degenerative tissues. This finding suggests that excessive calcium excitatory stimulation does not induce rapid neuronal degeneration and death. As found in previous studies, TUNEL tests and electron microscopy suggest that Purkinje cell degeneration and death are not occurring via an apoptotic process. These findings suggest that S bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Facultad de Ciencias Veterinaria

Immunohistochemical analysis of cytoskeleton and ubiquitin in cerebellum of poisoned cattle by Solanum bonariense

El Solanum bonariense L., es un arbusto perenne, autóctono en Uruguay, Argentina y sur de Brasil. Al ser ingerido por los bovinos en pastoreo, les ocasiona una degeneración cortical cerebelosa, afectando específicamente a las células de Purkinje. Estas presentan el pericario extensamente vacuolado con desplazamiento del núcleo, con progresiva muerte neuronal. En los axones de estas neuronas se observa presencia de esferoides axonales en la sustancia blanca cerebelosa, que finalmente son sustituidos por microcavitaciones en la sustancia blanca. El objetivo del presente trabajo fue describir el patrón de inmunoreactividad contra diferentes elementos del citoesqueleto y la ubiquitina conjugada a proteínas marcadas para degradarse dentro de las células de Purkinje de cerebelos de bovinos intoxicados natural o experimentalmente por ingestión de Solanum bonariense L.Facultad de Ciencias Veterinaria

Calbindin d 28k expression in the cerebellum of normal and solanum bonariense L. Intoxicated bovines

La ingestión natural o experimental de Solanum bonariense L. ocasiona una degeneración cortical cerebelosa en bovinos. Esta lesión se debe a la degeneración específica de las células de Purkinje, postulándose que la misma se debe a alteraciones metabólicas específicas, demostradas previamente por estudios ultraestructurales e inmunohistoquímicos. La Calbindina-28kD (Cb28k) ha sido considerada como un marcador específico de células de Purkinje. La alteración de esta inmunoreacción se vincula al desarrollo de diferentes enfermedades neurodegenerativas en humanos. El objetivo del presente trabajo fue analizar la expresión de Cb28k en cerebelos de bovinos normales y en los intoxicados con Solanum bonariense L.Facultad de Ciencias Veterinaria

Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines

Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell susceptibility. Calbindin D28k (CbD28k) is highly expressed in Purkinje cells and has been used as a marker for normal and degenerative Purkinje cells. The goal of this study was to describe S bonariense-induced disease by ascertaining Purkinje cell-specific degenerative changes using CbD28k expression and to correlate this with apoptosis in Purkinje cells, as determined using TUNEL (transferase-mediated dUTP-biotin nick end-labeling) and ultrastructural changes. In all cases, an increase in both dose and duration of S bonariense intoxication resulted in a decrease in the number of Purkinje cells. CbD28k immunohistochemistry was an excellent marker for Purkinje cells because immunoreactivity did not change in normal or degenerative tissues. This finding suggests that excessive calcium excitatory stimulation does not induce rapid neuronal degeneration and death. As found in previous studies, TUNEL tests and electron microscopy suggest that Purkinje cell degeneration and death are not occurring via an apoptotic process. These findings suggest that S bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Facultad de Ciencias Veterinaria

Tuberculosis is associated with expansion of a motile, permissive and immunomodulatory CD16(+) monocyte population via the IL-10/STAT3 axis

The human CD14+ monocyte compartment is composed by two subsets based on CD16 expression. We previously reported that this compartment is perturbed in tuberculosis (TB) patients, as reflected by the expansion of CD16+ monocytes along with disease severity. Whether this unbalance is beneficial or detrimental to host defense remains to be elucidated. Here in the context of active TB, we demonstrate that human monocytes are predisposed to differentiate towards an anti-inflammatory (M2-like) macrophage activation program characterized by theCD16+CD163+MerTK+pSTAT3+ phenotype and functional properties such as enhanced protease-dependent motility, pathogen permissivity and immunomodulation. This process is dependent on STAT3 activation, and loss-of-function experiments point towards a detrimental role in host defense against TB. Importantly, we provide a critical correlation between the abundance of the CD16+CD163+MerTK+pSTAT3+ cells and the progression of the disease either at the local level in a non-human primate tuberculous granuloma context, or at the systemic level through the detection of the soluble form of CD163 in human sera. Collectively, this study argues for the pathogenic role of the CD16+CD163+MerTK+pSTAT3+ monocyte-to-macrophage differentiation program and its potential as a target for TB therapy,and promotes the detection of circulating CD163 as a potential biomarker for disease progression and monitoringof treatment efficacy.Fil: Lastrucci, Claire. Centre National de la Recherche Scientifique; FranciaFil: Bénard, Alan. Centre National de la Recherche Scientifique; FranciaFil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pingris, Karine. Centre National de la Recherche Scientifique; FranciaFil: Souriant, Shanti. Centre National de la Recherche Scientifique; FranciaFil: Poincloux, Renaud. Centre National de la Recherche Scientifique; FranciaFil: Al Saati, Talal. Inserm; FranciaFil: Rasolofo, Voahangy. Pasteur Institute in Antananarivo; MadagascarFil: González Montaner, Pablo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas ; ArgentinaFil: Inwentarz, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas ; ArgentinaFil: Moraña, Eduardo José. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas ; ArgentinaFil: Kondova, Ivanela. Biomedical Primate Research Centre; Países BajosFil: Verreck, Franck A. W.. Biomedical Primate Research Centre; Países BajosFil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Neyrolles, Olivier. Centre National de la Recherche Scientifique; FranciaFil: Maridonneau Parini, Isabel. Centre National de la Recherche Scientifique; FranciaFil: Lugo Villarino, Geanncarlo. Centre National de la Recherche Scientifique; FranciaFil: Cougoule, Celine. Centre National de la Recherche Scientifique; Franci

Sepharadim/conversos and premodern Global Hispanism

Sepharadim participated in the Hispanic vernacular culture of the Iberian Peninsula. Even in the time of al-Andalus many spoke Hispano-Romance, and even their Hebrew literature belies a deep familiarity with and love of their native Hispano-Romance languages. However, since the early sixteenth century the vast majority of Sepharadim have never lived in the Hispanic world. Sepharadim lived not in Spanish colonies defined by Spanish conquest, but in a network of Mediterranean Jewish communities defined by diasporic values and institutions. By contrast, the conversos, those Sepharadim who converted to Catholicism, whether in Spain or later in Portugal, Italy, or the New World, lived mostly in Spanish Imperial lands, were officially Catholic, and spoke normative Castilian. Their connections, both real and imagined, with Sephardic cultural practice put them at risk of social marginalization, incarceration, even death. Some were devout Catholics whose heritage and family history doomed them to these outcomes. Not surprisingly, many Spanish and Portugese conversos sought refuge in lands outside of Spanish control where they might live openly as Jews. This exodus (1600s) from the lands formerly known as Sefarad led to a parallel Sephardic community of what conversos who re-embraced Judaism in Amsterdam and Italy by a generation of conversos trained in Spanish universities. The Sephardic/Converso cultural complex exceeds the boundaries of Spanish imperial geography, confuses Spanish, Portuguese, Catholic, and Jewish subjectivities, and defies traditional categories practiced in Hispanic studies, and are a unique example of the Global Hispanophone

Effectiveness of community-based initiatives for mitigation of land degradation after wildfires

Local communities, informed about the main post-fire environmental threats, applied land restoration techniques after the 2017 Ponte Caldelas wildfire (Galicia, Spain). Volunteers crews, supervised by local researchers/technicians iden- tified high-severity burned areas and applied: post-fire mulch by using corn mulch (chopped corn at a rate of 4 Mg ha−1), corn strip mulch (chopped corn on 1-m wide contourline-strips at the bottom of plots, at 1 Mg ha−1) and wheat mulch (wheat straw, at 2 Mg ha−1), as well as acorn seeding with native oak (Quercus robur) acorns. Further- more, the mulch effect on seed germination/root elongation was tested in the laboratory using direct topsoil samples and <0.45 microns-filtered soil solutions. Soil erosion during the first post-fire year was 27-11 Mg ha−1 for the Parada-Laforet sites, respectively. Corn, corn strip, and wheat mulching were very effective at reducing soil erosion, leading to 95, 76% and 93% less erosion than in untreated plots. During post-fire year 2 mulching was still effective at reducing soil erosion in the case of corn (87%) and corn strip mulch (73%), but less effective with straw mulch (36%). For the third year no differences were observed. Acorn seedling establishment failed in 96% of the cases. Still, survival rate of the germinated acorns was significantly higher on the mulched (50%) than on the untreated spots (26%; p<0.05). The laboratory experiments also showed higher germination rates, shoot length and biomass in mulched than untreated soils. The results demon- strated that the mulching measures applied by the local volunteers were effective for preventing soil erosion after for- est fires. The success of ecosystem restoration with acorn seeding was low due to the low germination and the high predation by rodents, and further research is needed to scale acorn seeding to natural recruitment rates.he contribution of the first author has been funded through FCT - Foundation for Science and Technology (Portugal) under the Project UIDB/05183/2020. The first author was supported by Research contracts (CDL-CTTRI-88-ARH/2018 REF.-138-88-ARH/2018) and Auxiliary research contract (CEECIND/01473/2020), funded by national funds (OE), through the Portuguese Foundation for Science and Technology (FCT/MCTES)