Modulación local de un canal operado por depósito

In T lymphocytes, the Ca2+ entry mainly occurs through a particular type of store-operated Ca2+ (SOC) channel known as ICRAC. These channels open as consequence of Ca2+ stores emptying which is induced by inositol trisphosphate action next to recognition of an antigen. Local Ca2+ microdomains act as negative feedback regulators of CRAC channels promoting inactivation processes. Mitochondria by serving as intracellular Ca2+ buffers have been indicated to control the activity of ICRAC, however, the possibility of a factor released from mitochondria have been also pointed out, but no direct evidences have obtained at this respect yet. Thus, metabolically competent respiring mitochondria were able to reduce Ca2+-mediated inhibition of CRAC channels in the presence of high concentrations of different intracellular chelators and inhibitors of the endoplasmic reticulum Ca2+-ATPase. Recently, our group has shown that this effect is mainly mediated by a soluble mitochondrial factor (ATP) than can act as calcium chelator. In this context, we have begun to study other elements than could to exert a local control in the activity of CRAC channels as protein kinase C, plasma membrane calcium ATPase or the contribution of the lipidic composition of plasma membrane

Multi-sensor system using plastic optical fibers for intrinsically safe level measurements

A system for measuring liquid level in multiple tanks using optical fibe technology has been developed. Oil fiel service industry or any sector requiring liquid level measurements in flammabl atmospheres can be benefite from this intrinsically safe technology. The device used a single lens for the emitting and receiving fibe and it is based on amplitude variations as a function of the liquid distance and not in time of fligh or phase detection. Being the firs fiber-opti liquid level sensor with those characteristics for long ranges (>200 cm). A simple model to describe their behavior has been derived and tested on two prototypes. A Monte-Carlo method is used to fi the experimental data and obtain the model parameters. High accuracy between experimental data and fitte curve is obtained. The prototypes have a good linearity, better than 1.5% FS (full scale). Sensor heads are made of plastic optical fiber (POF) that are easy to handle, flexible and economical. They are excited by 650 nm lasers, housed in ST-connectors to obtain compact and rough prototypes. Optical multiplexing is used to increase the measuring safety area. Frequency division multiplexing is used to address each sensor head. A discussion about the influenc of tilts and aberrations is also included.Publicad

Fed-batch control based upon the measurement of intracellular NADH

A series of experiments demonstrating that on-line measurements of intracellular NADH by culture fluorescence can be used to monitor and control the fermentation process are described. A distinct advantage of intercellular NADH measurements over other monitoring techniques such as pH and dissolved oxygen is that it directly measures real time events occurring within the cell rather than changes in the environment. When coupled with other measurement parameters, it can provide a finer degree of sophistication in process control

Investigación de mercado, elaboración de estrategias para la realización del relanzamiento con presupuestos reales caso: relanzamiento rapid juice

CEALCO, con su producto Pulpa de Fruta Congelada, que se comercializa con el nombre de “Rapid Juice”, tiene ya dos años en el mercado ecuatoriano. Entre los competidores más fuertes están: Maria Morena, La Jugosa y los jugos concentrados de Facundo. Para poder competir en el mercado, ante estos reconocidos competidores, Rapid Juice debe aplicar una estrategia que le permitirá aumentar sus ventas y su nivel de aceptación y reconocimiento de la marca por parte de los consumidores. Según este planteamiento, es sumamente importante para CEALCO manejar información de calidad, que sirva para lograr conocer aspectos fundamentales respecto a las características del mercado como: Establecer la situación real del mercado en la actualidad. Determinar si los actuales esfuerzos mercadológicos están correctamente encaminados. Identificar de manera exacta la dimensión del mercado. Reconocer las necesidades y las preferencias de los consumidores. Teniendo todos estos aspectos claros y basándose en los resultados obtenidos, se ha planteado dentro del proyecto, realizar un relanzamiento del producto mejorando sus características y atributos actuales así como la implementación de un nuevo plan de mercadeo, que permitirá mejorar su situación dentro del mercado de pulpa de frutas congeladas

Amadis of Gaul, Books I and II: A Novel of Chivalry of the 14th Century Presumably First Written in Spanish

Includes a new Introduction by John E. Keller In the long history of European prose fiction, few works have been more influential and more popular than the romance of chivalry Amadis of Gaul . Although its original author is unknown, it was probably written during the early fourteenth century. The first great bestseller of the age of printing, Amadis of Gaul was translated into dozens of languages and spawned sequels and imitators over the centuries. A handsome, valiant, and undefeatable knight, Amadis is perhaps best known today as Don Quixote’s favorite knight-errant and model. This exquisite English translation restores a masterpiece to print. A most welcome arrival. It\u27s availability opens up the possibility of a wider, general audience, and of teaching Amadis not only to the graduate comparatist, but also to the large undergraduate audience. -- Bulletin of Spanish Studies What made the original and its sequels international bestsellers in the early age of printing is bound to appeal to many modern readers. Amadis combines the sentimental with the chivalric, while adding bountiful measures of pure adventure and fantasy. -- La Coronica Set in and around England after the Roman era, but before the advent of Arthur, it tells the complex interwoven story of the adventures of Amadis, the greatest knight of all time. . . . This 16th century bestseller presents an exciting, past-paced and ultimately very entertaining story that modern readers should have little difficulty in appreciating. -- SF Sitehttps://uknowledge.uky.edu/upk_spanish_literature/1002/thumbnail.jp

Metformin-mediated increase in DICER1 regulates microRNA expression and cellular senescence

Metformin, an oral hypoglycemic agent, has been used for decades to treat type 2 diabetes mellitus. Recent studies indicate that mice treated with metformin live longer and have fewer manifestations of age-related chronic disease. However, the molecular mechanisms underlying this phenotype are unknown. Here, we show that metformin treatment increases the levels of the microRNA-processing protein DICER1 in mice and in humans with diabetes mellitus. Our results indicate that metformin upregulates DICER1 through a post-transcriptional mechanism involving the RNA-binding protein AUF1. Treatment with metformin altered the subcellular localization of AUF1, disrupting its interaction with DICER1 mRNA and rendering DICER1 mRNA stable, allowing DICER1 to accumulate. Consistent with the role of DICER1 in the biogenesis of microRNAs, we found differential patterns of microRNA expression in mice treated with metformin or caloric restriction, two proven life-extending interventions. Interestingly, several microRNAs previously associated with senescence and aging, including miR-20a, miR-34a, miR-130a, miR-106b, miR-125, and let-7c, were found elevated. In agreement with these findings, treatment with metformin decreased cellular senescence in several senescence models in a DICER1- dependent manner. Metformin lowered p16 and p21 protein levels and the abundance of inflammatory cytokines and oncogenes that are hallmarks of the senescence-associated secretory phenotype (SASP). These data lead us to hypothesize that changes in DICER1 levels may be important for organismal aging and to propose that interventions that upregulate DICER1 expression (e.g., metformin) may offer new pharmacotherapeutic approaches for age-related disease

Cal Poly Rose Float Overheight Mechanism

The Cal Poly Universities jointly build and enter a floral entry, commonly known as a float, into the Pasadena Tournament of Roses Rose Parade. At the end of this parade route, there is a 16’6” bridge all floats must drive under. The scope of our project is to design and build a mechanism roughly described as an “overheight” mechanism, as its function is to raise and lower large heavy structures so the float is able to pass under the bridge. This hydraulic mechanism is powered via the float animation system

Multi-criteria analysis applied to multi-objective optimal pump scheduling in water systems

[EN] This work presents a multi-criteria-based approach to automatically select specific non-dominated solutions from a Pareto front previously obtained using multi-objective optimization to find optimal solutions for pump control in a water supply system. Optimal operation of pumps in these utilities is paramount to enable water companies to achieve energy efficiency in their systems. The Fuzzy Technique for Order of Preference by Similarity to Ideal Solution (FTOPSIS) is used to rank the Pareto solutions found by the non-dominated sorting genetic algorithm (NSGA-II) employed to solve the multi-objective problem. Various scenarios are evaluated under leakage uncertainty conditions, resulting in fuzzy solutions for the Pareto front. This paper shows the suitability of the approach for quasi real-world problems. In our case-study, the obtained solutions for scenarios including leakage represent the best trade-off among the optimal solutions, under some considered criteria, namely, operational cost, operational lack of service, pressure uniformity and network resilience. Potential future developments could include the use of clustering alternatives to evaluate the goodness of each solution under the considered evaluation criteria.Carpitella, S.; Brentan, BM.; Montalvo Arango, I.; Izquierdo Sebastián, J.; Certa, A. (2019). Multi-criteria analysis applied to multi-objective optimal pump scheduling in water systems. Water Science & Technology: Water Supply. 19(8):2338-2346. https://doi.org/10.2166/ws.2019.115S23382346198Ancău, M., & Caizar, C. (2010). The computation of Pareto-optimal set in multicriterial optimization of rapid prototyping processes. Computers & Industrial Engineering, 58(4), 696-708. doi:10.1016/j.cie.2010.01.015Aşchilean, I., Badea, G., Giurca, I., Naghiu, G. S., & Iloaie, F. G. (2017). Choosing the Optimal Technology to Rehabilitate the Pipes in Water Distribution Systems Using the AHP Method. Energy Procedia, 112, 19-26. doi:10.1016/j.egypro.2017.03.1109Brentan, B., Meirelles, G., Luvizotto, E., & Izquierdo, J. (2018). Joint Operation of Pressure-Reducing Valves and Pumps for Improving the Efficiency of Water Distribution Systems. Journal of Water Resources Planning and Management, 144(9), 04018055. doi:10.1061/(asce)wr.1943-5452.0000974Certa, A., Enea, M., Galante, G. M., & La Fata, C. M. (2017). ELECTRE TRI-based approach to the failure modes classification on the basis of risk parameters: An alternative to the risk priority number. Computers & Industrial Engineering, 108, 100-110. doi:10.1016/j.cie.2017.04.018Chen, C.-T. (2000). Extensions of the TOPSIS for group decision-making under fuzzy environment. Fuzzy Sets and Systems, 114(1), 1-9. doi:10.1016/s0165-0114(97)00377-1Cruz-Reyes, L., Fernandez, E., Sanchez, P., Coello Coello, C. A., & Gomez, C. (2017). Incorporation of implicit decision-maker preferences in multi-objective evolutionary optimization using a multi-criteria classification method. Applied Soft Computing, 50, 48-57. doi:10.1016/j.asoc.2016.10.037Farmani, R., Ingeduld, P., Savic, D., Walters, G., Svitak, Z., & Berka, J. (2007). Real-time modelling of a major water supply system. Proceedings of the Institution of Civil Engineers - Water Management, 160(2), 103-108. doi:10.1680/wama.2007.160.2.103Hadas, Y., & Nahum, O. E. (2016). Urban bus network of priority lanes: A combined multi-objective, multi-criteria and group decision-making approach. Transport Policy, 52, 186-196. doi:10.1016/j.tranpol.2016.08.006Hamdan, S., & Cheaitou, A. (2017). Supplier selection and order allocation with green criteria: An MCDM and multi-objective optimization approach. Computers & Operations Research, 81, 282-304. doi:10.1016/j.cor.2016.11.005Ho, W. (2008). Integrated analytic hierarchy process and its applications – A literature review. European Journal of Operational Research, 186(1), 211-228. doi:10.1016/j.ejor.2007.01.004Jowitt, P. W., & Germanopoulos, G. (1992). Optimal Pump Scheduling in Water‐Supply Networks. Journal of Water Resources Planning and Management, 118(4), 406-422. doi:10.1061/(asce)0733-9496(1992)118:4(406)Jowitt, P. W., & Xu, C. (1990). Optimal Valve Control in Water‐Distribution Networks. Journal of Water Resources Planning and Management, 116(4), 455-472. doi:10.1061/(asce)0733-9496(1990)116:4(455)Kurek, W., & Ostfeld, A. (2013). Multi-objective optimization of water quality, pumps operation, and storage sizing of water distribution systems. Journal of Environmental Management, 115, 189-197. doi:10.1016/j.jenvman.2012.11.030Lima, G. M., Luvizotto, E., & Brentan, B. M. (2017). Selection and location of Pumps as Turbines substituting pressure reducing valves. Renewable Energy, 109, 392-405. doi:10.1016/j.renene.2017.03.056Mala-Jetmarova, H., Sultanova, N., & Savic, D. (2017). Lost in optimisation of water distribution systems? A literature review of system operation. Environmental Modelling & Software, 93, 209-254. doi:10.1016/j.envsoft.2017.02.009Montalvo, I., Izquierdo, J., Pérez-García, R., & Herrera, M. (2014). Water Distribution System Computer-Aided Design by Agent Swarm Optimization. Computer-Aided Civil and Infrastructure Engineering, 29(6), 433-448. doi:10.1111/mice.12062Odan, F. K., Ribeiro Reis, L. F., & Kapelan, Z. (2015). Real-Time Multiobjective Optimization of Operation of Water Supply Systems. Journal of Water Resources Planning and Management, 141(9), 04015011. doi:10.1061/(asce)wr.1943-5452.0000515Ostfeld, A., Uber, J. G., Salomons, E., Berry, J. W., Hart, W. E., Phillips, C. A., … Walski, T. (2008). The Battle of the Water Sensor Networks (BWSN): A Design Challenge for Engineers and Algorithms. Journal of Water Resources Planning and Management, 134(6), 556-568. doi:10.1061/(asce)0733-9496(2008)134:6(556)Todini, E. (2000). Looped water distribution networks design using a resilience index based heuristic approach. Urban Water, 2(2), 115-122. doi:10.1016/s1462-0758(00)00049-2Zaidan, A. A., Zaidan, B. B., Al-Haiqi, A., Kiah, M. L. M., Hussain, M., & Abdulnabi, M. (2015). Evaluation and selection of open-source EMR software packages based on integrated AHP and TOPSIS. Journal of Biomedical Informatics, 53, 390-404. doi:10.1016/j.jbi.2014.11.012Żak, J., & Kruszyński, M. (2015). Application of AHP and ELECTRE III/IV Methods to Multiple Level, Multiple Criteria Evaluation of Urban Transportation Projects. Transportation Research Procedia, 10, 820-830. doi:10.1016/j.trpro.2015.09.03

Enfermería en Cartagena 1900-1920

With de objective of establishing the development and the process of turning nurses into professionals in Cartagena de Indias and to explain the place and challenges that women faced in the process to get this knowledge during the first two decades of the twentieth century, a qualitative research of historical type was conducted. The collection of information was centered on the systematic review of primary sources of the studied period (1920-1920), because this was the period of training nurses in the city and under the social-historical process of Cartagena.Con el fin de establecer el proceso y desarrollo de la profesionalización de la enfermería en Cartagena de Indias y explicar el lugar y los desafíos que la mujer enfrentó en los avances de este saber durante las dos primeras décadas del siglo XX, se realizó una investigación cualitativa de corte histórico. La recolección de información se circunscribió a la revisión sistemática de fuentes primarias del período en estudio (1900-1920), por ser este el período de formación de las Enfermeras en Cartagena, a la luz del proceso histórico-social de la ciudad de Cartagena

Volatility of methylglyoxal cloud SOA formed through OH radical oxidation and droplet evaporation

The volatility of secondary organic aerosol (SOA) formed through cloud processing (aqueous hydroxyl radical ( OH) oxidation and droplet evaporation) of methylglyoxal (MGly) was studied. Effective vapor pressure and effective enthalpy of vaporization (ΔH vap,eff ) were determined using 1) droplets containing MGly and its oxidation products, 2) a Vibrating Orifice Aerosol Generator (VOAG) system, and 3) Temperature Programmed Desorption Aerosol-Chemical Ionization Mass Spectrometry (TPD Aerosol-CIMS). Simulated in-cloud MGly oxidation (for 10–30 min) produces an organic mixture of higher and lower volatility components with an overall effective vapor pressure of (4 ± 7) × 10−7 atm at pH 3. The effective vapor pressure decreases by a factor of 2 with addition of ammonium hydroxide (pH 7). The fraction of organic material remaining in the particle-phase after drying was smaller than for similar experiments with glycolaldehyde and glyoxal SOA. The ΔH vap,eff of pyruvic acid and oxalic acid + methylglyoxal in the mixture (from TPD Aerosol-CIMS) were smaller than the theoretical enthalpies of the pure compounds and smaller than that estimated for the entire precursor/product mix after droplet evaporation. After 10–30 min of aqueous oxidation (one cloud cycle) the majority of the MGly +  OH precursor/product mix (even neutralized) will volatilize during droplet evaporation; neutralization and at least 80 min of oxidation at 10−12 M OH (or >12 h at 10−14 M) is needed before low volatility ammonium oxalate exceeds pyruvate