Goal-oriented behavioural and environmental enrichment in aquarium species

Environmental enrichment is a common tool used in zoo and aquarium settings to provide mental and physical stimulation to animals under human care. This paper aims to present a primary approach to goal-oriented environmental and behavioural enrichment following a simplified version of the SPIDER method for a diverse range of aquarium-housed species. The aim of the proposed goal-oriented environmental-enrichment programme is to stimulate behavioural diversity by providing controlled stimuli. This programme is easy to implement and can be used by animal care staff; it encompasses an easy evaluation form for daily monitoring and seeks to increase species-specific behavioural diversity. It is suggested that environmental enrichment programmes should be designed for the benefit of animal welfare; understanding how they affect animals under human care is essential to take informed decisions about which behaviours or situations to stimulate. An environmental enrichment protocol was developed which considers the ethology and biology of the species under consideration prior to implementation. Specific goals (i.e., behaviours and/or actions) were assigned to particular stimuli (enrichment devices) and the efficacy of the enrichment devices were assessed by reporting on the reactions of the animals. Over time, this protocol has made it possible to define a validated catalogue of enrichment devices for which specific goals and expected reactions are consistent. The increased behavioural diversity achieved with this programme is providing aquatic species with the choice to display species-specific behaviours

Clinical, radiographic and histological findings of seven teeth from two California sea lions (Zalophus californianus) housed under professional care

Seven teeth extracted from two adult California sea lions (Zalophus californianus) due to pulp exposure and/or to gain access to the mandibular canine teeth were histologically evaluated, and the findings were compared with clinical and radiographic findings. Three teeth were diagnosed with pulp exposure, and two of these showed no radiographic signs of endodontic disease and were histologically vital with prominent coronal pulpitis and a pulp polyp. Another tooth with pulp exposure was showing clinical and radiographic signs of endodontic disease and was histologically confirmed with pulp necrosis. A discoloured incisor tooth was showing radiographic signs of endodontic disease and was also histologically non-vital. Two clinically and radiographically healthy mandibular first premolar teeth and one second incisor tooth had no evidence of pulpitis or pulp necrosis but had pulp canal obliteration. Regular clinical and radiographic follow-up for 5 months to 3 years after the procedures confirmed uneventful healing of the extraction sites, despite initial flap’s dehiscence. Although extractions of affected teeth in California sea lions are considered the most practical and beneficial therapy, these are associated with the risks of extensive trauma and anaesthesia and the need to perform these surgical procedures on-site under variable conditions. As California sea lions can be trained to allow conscious dental radiographic re-checks, monitoring teeth with clinical signs of pulp polyp formation and without radiographic signs of endodontic disease warrant further evaluation/reconsideration from previous recommendations. Endodontic treatment of abscessed teeth in California sea lions is reportedly unsuccessful and is discouraged. However, vital pulpectomy could be an alternative treatment to extraction in teeth with pulp polyps as it was found to be highly successful in humans, but the possibility of endodontic failure and need for further treatments should be weighted in the treatment choice

Dolphin-WET—Development of a Welfare Evaluation Tool for Bottlenose Dolphins (Tursiops truncatus) under Human Care

Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor’s Five Domains Model and the Welfare Quality®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively

Table_1_Clinical, radiographic and histological findings of seven teeth from two California sea lions (Zalophus californianus) housed under professional care.DOCX

Seven teeth extracted from two adult California sea lions (Zalophus californianus) due to pulp exposure and/or to gain access to the mandibular canine teeth were histologically evaluated, and the findings were compared with clinical and radiographic findings. Three teeth were diagnosed with pulp exposure, and two of these showed no radiographic signs of endodontic disease and were histologically vital with prominent coronal pulpitis and a pulp polyp. Another tooth with pulp exposure was showing clinical and radiographic signs of endodontic disease and was histologically confirmed with pulp necrosis. A discoloured incisor tooth was showing radiographic signs of endodontic disease and was also histologically non-vital. Two clinically and radiographically healthy mandibular first premolar teeth and one second incisor tooth had no evidence of pulpitis or pulp necrosis but had pulp canal obliteration. Regular clinical and radiographic follow-up for 5 months to 3 years after the procedures confirmed uneventful healing of the extraction sites, despite initial flap’s dehiscence. Although extractions of affected teeth in California sea lions are considered the most practical and beneficial therapy, these are associated with the risks of extensive trauma and anaesthesia and the need to perform these surgical procedures on-site under variable conditions. As California sea lions can be trained to allow conscious dental radiographic re-checks, monitoring teeth with clinical signs of pulp polyp formation and without radiographic signs of endodontic disease warrant further evaluation/reconsideration from previous recommendations. Endodontic treatment of abscessed teeth in California sea lions is reportedly unsuccessful and is discouraged. However, vital pulpectomy could be an alternative treatment to extraction in teeth with pulp polyps as it was found to be highly successful in humans, but the possibility of endodontic failure and need for further treatments should be weighted in the treatment choice.</p

Dolphin Welfare Assessment under Professional Care: ‘Willingness to Participate’, an Indicator Significantly Associated with Six Potential ‘Alerting Factors’

International audienceIn dolphinaria, dolphins and their trainers build relationships and bonds due to the nature,closeness and repeatability of their interactions, hence training sessions are deemed appropriate toevaluate dolphin welfare. Qualitative Behavioural Assessments (QBAs) have been used to studyhuman–animal relationships and are included in several animal welfare assessments. We introducehere the first QBA aiming to analyse dolphin–trainer interactions during training sessions in terms ofdolphin welfare. Our results show that “Willingness to Participate” (WtP) was significantly associatedto six other parameters: high-speed approach, high level of excitement, high number of positiveresponses to trainers’ signals, rare refusal to perform certain behaviours, rare spontaneous departurebehaviours and fast approach once the trainer entered into the pool. Therefore, we suggest usingWtP and those “alerting factors” when assessing dolphin–trainer interactions under professionalcare. The evaluation should also consider the time of day, the dolphin’s age, trainer experience level,the nature of the training sessions and to a lesser extent the sex of the dolphins, as contributing andmodulating factors. The factor eye contact has been used in various HARs studies and has beenproven to be a valid indicator in welfare research works, hence potentially deserving further research.These results demonstrate the pertinence and feasibility of this approach, the ease of use of thismethodology by professionals in zoo/aquarium settings and the appropriateness of the obtainedresults within the holistic frame of animal welfare

Cross-sectional anatomy, computed tomography and magnetic resonance imaging of the head of common dolphin (Delphinus delphis) and striped dolphin (Stenella coeruleoalba)

Computed tomography (CT) and low-field magnetic resonance imaging (MRI) were used to scan seven by-caught dolphin cadavers, belonging to two species: four common dolphins (Delphinus delphis) and three striped dolphins (Stenella coeruleoalba). CT and MRI were obtained with the animals in ventral recumbency. After the imaging procedures, six dolphins were frozen at 20°C and sliced in the same position they were examined. Not only CT and MRI scans, but also cross sections of the heads were obtained in three body planes: transverse (slices of 1 cm thickness) in three dolphins, sagittal (5 cm thickness) in two dolphins and dorsal (5 cm thickness) in two dolphins. Relevant anatomical structures were identified and labelled on each cross section, obtaining a comprehensive bi-dimensional topographical anatomy guide of the main features of the common and the striped dolphin head. Furthermore, the anatomical cross sections were compared with their corresponding CT and MRI images, allowing an imaging identification of most of the anatomical features. CT scans produced an excellent definition of the bony and air-filled structures, while MRI allowed us to successfully identify most of the soft tissue structures in the dolphin’s head. This paper provides a detailed anatomical description of the head structures of common and striped dolphins and compares anatomical cross sections with CT and MRI scans, becoming a reference guide for the interpretation of imaging studies

Behavioural diversity study in bottlenose dolphin (Tursiops truncatus) groups and its implications for welfare assessments

In the recent past, animal welfare studies have tried to determine the best animal welfare measures and indicators. Expression of behavioural diversity is considered a potential positive welfare indicator, and to the authors’ knowledge, it has not been validated nor studied in cetaceans. For the first time, a behavioural diversity study on bottlenose dolphins (Tursiops truncatus) groups was conducted at six European facilities. The study was carried out by the animal care staff, biologists and veterinarians and included 54 dolphins housed in several group compositions at the different participating facilities. The goal of our study was to analyse behavioural diversity in bottlenose dolphins at the group level to investigate how particular factors might impact the diversity of behaviours within the group and to discuss its implications for dolphin welfare assessments. Eight factors (i.e., “observer location”, “number of individuals”, “age class”, “sex”, “social grouping”, “presence/absence of leading male”, “presence/absence of visitors” and “enrichment provision”) impacted the behavioural diversity of the observed groups, while no significant impact of the factors “time of day” and “activity before/after observation” could be found. Our study showed the feasibility of this kind of approach for cetaceans under professional care and the relevance to considering this parameter in dolphin welfare studies, despite certain limitations that warrant further research

Dolphin-WET—Development of a Welfare Evaluation Tool for Bottlenose Dolphins (<i>Tursiops truncatus</i>) under Human Care

Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor’s Five Domains Model and the Welfare Quality®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively

Dolphin-WET-Development of a Welfare Evaluation Tool for Bottlenose Dolphins (Tursiops truncatus) under Human Care

The welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus) under human care named Dolphin-WET. The tool encompasses 49 indicators (i.e., 37 animal-based and 12 resource-based indicators) inspired by Mellor's Five Domains Model and the Welfare Quality ®. The Dolphin-WET is a species-specific and individual-based welfare assessment tool that provides a holistic approach to evaluating bottlenose dolphins' welfare. Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor's Five Domains Model and the Welfare Quality ®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively