Analysis of the functional connection between iron uptake regulation and ethylene signalling in Arabidopsis thaliana

Eisen ist durch die Fähigkeit zur reversiblen Redoxreaktion der wichtigste Mikronährstoff für Pflanzen. Es ist vor allem als Kofaktor von Enzymen beteiligt an lebenswichtigen Reaktionen. Eisen muss von der Pflanze aktiv aus dem Boden gelöst, reduziert und aufgenommen werden. Die Aufnahme in die Pflanze wird aufgrund der hohen Reaktivität und der damit verbundenen Risiken genau gesteuert. In Arabidopsis thaliana, der wichtigsten Modellpflanze der Molekularbiologie, sind für Reduktion und Aufnahme aus dem Boden die beiden Proteine AtFRO2 als Reduktase und AtIRT1 als Transporter zuständig. Sie werden transkriptionell in Abhängigkeit des Eisenstatus der Pflanze durch den BHLH Transkriptionsfaktor AtFIT gesteuert. AtFIT jedoch wird nur schwach transkriptionell reguliert, eine weitere Regulation findet auf posttranskriptioneller Ebene statt. Eine Möglichkeit dafür ist die Dimerisierung mit anderen Proteinen. In der vorliegenden Arbeit konnte eine Proteininteraktion von AtFIT mit den Transkriptionsfaktoren AtEIN3 und AtEIL1 aus dem Signalweg des Pflanzenhormons Ethylen in planta verifiziert werden. Die Untersuchung des Einflusses von AtEIN3 und AtEIL1 ergab, dass diese mit AtFIT additiv als positive Regulatoren in der Eisenmangelantwort agieren. Weitere Untersuchungen mit Faktoren aus dem Ethylensignalweg sowie mit Ethylen ergaben, dass Ethylen eine wichtige Rolle in der Regulation der Eisenhomöostase spielt.Iron is, due to its ability for reversible redox reactions, the most important micronutrient for plants. It serves primarily as cofactor in enzymes which are involved in pivotal reactions. Iron needs actively to be solved from the soil, be reduced and taken up by the plant. This mechanism has to be tightly controlled due to the high reactivity of iron and the resulting risks. In the model plant Arabidopsis thaliana, the proteins AtFRO2 as reductase and AtIRT1 as transporter are responsible for the reduction and uptake from the soil. Both are regulated in a transcriptional manner dependent on the iron status of the plant by the BHLH transcription facor AtFIT. AtFIT itself is only weakly regulated on transcriptional level, further regulation takes place at the posttranscriptional level. One possibility for this regulation is the dimerisation with other proteins. In the present work, a protein interaction of AtFIT with the transcription factors AtEIN3 and AtEIL1 from the signalling pathway of the hormone ethylene could be verified in planta. Analysis of the influence of AtEIN3 and AtEIL1 revealed their roles as positive regulators together with AtFIT in an additive manner. Further experiments with other factors from the ethylene signalling pathway and with ethylene itself showed that ethylene plays an important role in the iron homeostasis of the plant

Because Data Shall Grow (and we With it)

Rakers J, Miller B, Mohrbacher J, et al. Because Data Shall Grow (and we With it). Presented at the 1st Conference on Research Data Infrastructure - Connecting Communities, Karlsruhe, Germany.Poster for conference abstract: https://doi.org/10.52825/cordi.v1i.278 Research data are a valuable asset of their own and individual researchers as well as the research community as a whole can benefit through data sharing practices and open science. These benefits include but are not limited to higher data quality or the more efficient use of time and financial resources. Despite these potential gains, data sharing is not widespread yet and processes of cultural change are needed to reap the benefits of data sharing. Against this background, the NFDI can function as a platform for discussion and provides a network that extends beyond individual research bubbles in the name of common interests and facilitate cultural change processes towards data sharing. A first collaborative workshop in April 2023 identified four central clusters of interest including 1. policies, strategies and funding; 2. communities, workshops and multipliers; 3. publications and 4. collaboration, communication and error cultures. Departing from this, we contemplate developing a vision on how to further encourage cultural change in the NFDI through a series of workshops. As a result, a vision of cultural change within and with the NFDI will be published by the end of 2023 to further guide the processes to facilitate cultural change. A first step could be to change the guiding metaphor for data: Not &ldquo;Gold&rdquo; or &ldquo;Oil&rdquo;, but &ldquo;Humus&rdquo; for healthy growth of a collaborative data ecosystem.</p

Interaction between the bHLH Transcription Factor FIT and ETHYLENE INSENSITIVE3/ETHYLENE INSENSITIVE3-LIKE1 Reveals Molecular Linkage between the Regulation of Iron Acquisition and Ethylene Signaling in Arabidopsis[C][W]

A direct molecular link between ethylene signaling component EIN3/EIL1 and the iron deficiency response regulator FIT is shown. EIN3 and EIL1 were found to be required for Fe deficiency responses and FIT abundance. In the absence of EIN3/EIL1, FIT was more prone to proteasomal degradation. The ein3 eil1 transcriptome was affected to a greater extent upon iron deficiency compared with the wild type