Eotaxin, RANTES and tumor necrosis factor alpha levels in allergic rhinitis

AbstractObjectivesThe objectives of this study were to estimate the levels of eotaxin, RANTES and tumor necrosing factor-∞ in allergic rhinitis and their relation to disease severity.Study designProspective study.SettingMansoura University Hospital.Patients and methodsTwenty nine patients suffering from allergic rhinitis were included in this study (19 patients with allergic rhinitis and 10 patients control group). The patients underwent estimation of eotaxin, RANTES, TNF-∞ in the nasal wash using Elisa technique. The patients were divided according to the disease severity into mild allergic rhinitis, severe allergic rhinitis and control groups.ResultsThe mean values of eotaxin, RANTES, TNF-∞ in severe allergic rhinitis (33.6±11.07pg/ml, 72.17±87.61pg/ml, 25.47±4.04pg/ml) were statistically higher than in mild allergic rhinitis (9.80±6.79pg/ml, 10.50±6.90pg/ml, 12.99±3.27pg/ml) and the mean values of all these parameters were higher in both groups compared to control group (0.6±0.69pg/ml, 0.65±0.74pg/ml, 0.63±0.54pg/ml).ConclusionThis study suggests the role of local chemokines in the pathogenesis of allergic rhinitis as well as their possible relation to the severity of the disease which may direct the attention to therapeutic trials against these locally produced chemokines

Modified dome shaped proximal tibial osteotomy for treatment of infantile tibia vara

Background: Tibial deformity in childhood is not always one plane deformity, but often combines torsional and angular malalignment.Methods: In this prospective study, dome shaped osteotomy was performed in 36 tibiae in 24 patients. The osteotomy was held with K-wires and a plaster cast. The mean age at surgery was 5.5 years and the mean follow-up time was 30 months, range (24-42) months.Results: All osteotomies united and no compartment syndrome occurred. Postoperatively, one leg (2.7%) had temporary weakness of external hallucis longus muscle. Thirty five of thirty six legs had good clinical and radiological correction of alignment. Recurrent deformity was seen in one leg. ‎ Conclusions: Dome shaped osteotomy of the tibia is a simple, safe and effective method for correction of uni-planar varus deformity. For bi-planar deformities in childhood with infantile tibia vara we may need to step cut the lateral cortex to allow good bone contact to help in union with minimum morbidity.

Ultrastructure of the anterior adhesive apparatus of the gill parasite Macrogyrodactylus clarii and skin parasite M. congolensis (Monogenea; Gyrodactylidae) from the catfish Clarias gariepinus

Transmission electron microscopy (TEM) was used for the first time to study the anterior adhesive apparatus of the monogeneans Macrogyrodactylus clarii Gussev, 1961 and M. congolensis (Prudhoe, 1957) Yamaguti, 1963 inhabiting gills and skin respectively of the same catfish Clarias gariepinus. Despite the different microhabitats occupied by these parasites, the present study revealed that they have a similar anterior adhesive system. In both parasites, the anterior adhesive apparatus consists of three types of gland cells: G1 cells that produce rod-shaped bodies (S1), G2 cells manufacture irregularly shaped bodies (S2) and G3 cells form mucoid-like secretions (S3). In the cytoplasm of G1 cells, a single layer of microtubules encloses each developing rod-shaped body. A unique feature of S1 secretory bodies is that some fully developed S1 bodies are attached to each other, forming large condensed globules in the cytoplasm of G1 gland cells and terminal portion of the G1 ducts, but none were detected in the adhesive sacs outside the ducts. In the adhesive sacs, G1 ducts open with multiple apertures whereas each of the G2 and G3 ducts have a single opening. The adhesive sacs are lined with two types of tegument (st1 and st2). A third tegument type (st3) connects the st2 tegument with the general body tegument. Only st1 has microvilli. Each adhesive sac is provided with a spike-like sensillum and single uniciliated sense organ. The possible functions of microvilli in increasing the surface area and assistance in spreading and mixing of the adhesive secretion, and the role of sense organs associated with the adhesive sacs are discussed

Surgical Management of Split Depression Fracture of The Lateral Tibial Plateau

Background: Tibial plateau fractures are one of the commonest intra-articular fractures resulting from indirect coronal or direct axial compressive forces. Fractures of tibial plateau constitute 1% of all fractures and 8% of fractures in the elderly. Objective: In the present study, we aimed to prevent the development of osteoarthritis and to correlate the radiological findings with this type of tibial plateau fracture.Patients and methods: This study was an interventional study, where 18 patients were conducted with tibial plateau fracture Schatzker type 2 and were operated in the Department of Orthopedics, Faculty of Medicine, at Zagazig University Hospital. Results: Most of cases (55.6%) were operated upon 2 days after occurrence of fracture, followed by 27.8%, 16.6% were operated upon after 7 days and 10 days respectively. Seventeen (94.4%) cases were fixed by buttress plate while 1 case (5.6%) was fixed by proximal tibial plate and 16 cases (88.9%) needed bone graft. Significant improvement in time regard function score (knee society score) and radiological score (Rasmussen Assessment score) were found. No complication was found in 66.7% of studied group; 16.7% had infection, 11.1% had valgus deformity and only one case 5.6% had stiffness.Conclusion: It could be concluded that the surgical management of tibial plateau fractures is challenging and gives excellent anatomical reduction and rigid fixation to restore articular congruity, facilitate early knee motion by reducing post-traumatic osteoarthritis and thus achieving optimal knee function

Interferon-induced protein with tetratricopeptide repeats 5 of black fruit bat (Pteropus alecto) displays a broad inhibition of RNA viruses

Bats are natural host reservoirs and have adapted a unique innate immune system that permits them to host many viruses without exhibiting symptoms. Notably, bat interferon stimulated genes (ISGs) have been shown to play antiviral roles. Interferon induced protein with tetratricopeptide repeats 5 (IFIT5) is a well-characterised ISG in humans with antiviral activities against negative-sense RNA viruses via inhibiting viral transcription. Here, we aim to investigate if Pteropus alecto (pa) IFIT5 (paIFIT5) possess the ability to inhibit negative-sense RNA viruses. Initially, gene syntenic and comparative structural analyses of multiple animals highlighted a high level of similarity between Pteropus alecto and human IFIT5 proteins. Our results showed that paIFIT5 was significantly inducible by viral and dsRNA stimulation. Transient overexpression of paIFIT5 inhibited the replication of vesicular stomatitis virus (VSV). Using minireplicon and transcription reporter assays, we demonstrated the ability of paIFIT5 specifically to inhibit H17N10 polymerase activity. Mechanistically, we noticed that the antiviral potential of paIFIT5 against negative sense RNA viruses was retributed to its interaction with 5’ppp containing RNA. Taken together, these findings highlight the genetic and functional conservation of IFIT5 among mammal

Deep Structural Analysis of Myriads of Omicron Sub-Variants Revealed Hotspot for Vaccine Escape Immunity

The emergence of the Omicron variant has reinforced the importance of continued SARS-CoV-2 evolution and its possible impact on vaccine effectiveness. Specifically, mutations in the receptor-binding domain (RBD) are critical to comprehend the flexibility and dynamicity of the viral interaction with the human agniotensin-converting enzyme 2 (hACE2) receptor. To this end, we have applied a string of deep structural and genetic analysis tools to map the substitution patterns in the S protein of major Omicron sub-variants (n = 51) with a primary focus on the RBD mutations. This head-to-head comparison of Omicron sub-variants revealed multiple simultaneous mutations that are attributed to antibody escape, and increased affinity and binding to hACE2. Our deep mapping of the substitution matrix indicated a high level of diversity at the N-terminal and RBD domains compared with other regions of the S protein, highlighting the importance of these two domains in a matched vaccination approach. Structural mapping identified highly variable mutations in the up confirmation of the S protein and at sites that critically define the function of the S protein in the virus pathobiology. These substitutional trends offer support in tracking mutations along the evolutionary trajectories of SAR-CoV-2. Collectively, the findings highlight critical areas of mutations across the major Omicron sub-variants and propose several hotspots in the S proteins of SARS-CoV-2 sub-variants to train the future design and development of COVID-19 vaccines

The structural complexity of the Gammaproteobacteria flagellar motor is related to the type of its torque-generating stators

The bacterial flagellar motor is a cell-envelope-embedded macromolecular machine that functions as a propeller to move the cell. Rather than being an invariant machine, the flagellar motor exhibits significant variability between species, allowing bacteria to adapt to, and thrive in, a wide range of environments. For instance, different torque- generating stator modules allow motors to operate in conditions with different pH and sodium concentrations and some motors are adapted to drive motility in high-viscosity environments. How such diversity evolved is unknown. Here we use electron cryo-tomography to determine the in situ macromolecular structures of the flagellar motors of three Gammaproteobacteria species: Legionella pneumophila, Pseudomonas aeruginosa, and Shewanella oneidensis MR-1, providing the first views of intact motors with dual stator systems. Complementing our imaging with bioinformatics analysis, we find a correlation between the stator system of the motor and its structural complexity. Motors with a single H+-driven stator system have only the core P- and L-rings in their periplasm; those with dual H+-driven stator systems have an extra component elaborating their P-ring; and motors with Na+- (or dual Na+-H+)- driven stator systems have additional rings surrounding both their P- and L-rings. Our results suggest an evolution of structural complexity that may have enabled pathogenic bacteria like L. pneumophila and P. aeruginosa to colonize higher-viscosity environments in animal hosts

Chickens Expressing IFIT5 Ameliorate Clinical Outcome and Pathology of Highly Pathogenic Avian Influenza and Velogenic Newcastle Disease Viruses

Innate antiviral immunity establishes first line of defense against invading pathogens through sensing their molecular structures such as viral RNA. This antiviral potential of innate immunity is mainly attributed to a myriad of IFN-stimulated genes (ISGs). Amongst well-characterized ISGs, we have previously shown that antiviral potential of chicken IFN-induced proteins with tetratricopeptides repeats 5 (chIFIT5) is determined by its interaction potential with 5′ppp containing viral RNA. Here, we generated transgenic chickens using avian sarcoma-leukosis virus (RCAS)-based gene transfer system that constitutively and stably express chIFIT5. The transgenic chickens infected with clinical dose (EID50 104 for HPAIV and 105 EID50 for vNDV) of high pathogenicity avian influenza virus (HPAIV; H5N1) or velogenic strain of Newcastle disease virus (vNDV; Genotype VII) showed marked resistance against infections. While transgenic chickens failed to sustain a lethal dose of these viruses (EID50 105 for HPAIV and 106 EID50 for vNDV), a delayed and lower level of clinical disease and mortality, reduced virus shedding and tissue damage was observed compared to non-transgenic control chickens. These observations suggest that stable expression of chIFIT5 alone is potentially insufficient in providing sterile protection against these highly virulent viruses; however, it is sufficient to ameliorate the clinical outcome of these RNA viruses. These findings propose the potential of innate immune genes in conferring genetic resistance in chickens against highly pathogenic and zoonotic viral pathogens causing sever disease in both animals and humans