S-Mbank: Secure Mobile Banking Authentication Scheme Using Signcryption, Pair Based Text Authentication, and Contactless Smartcard

Nowadays, mobile banking becomes a popular tool which consumers can conduct financial transactions such as shopping, monitoring accounts balance, transferring funds and other payments. Consumers dependency on mobile needs, make people take a little bit more interest in mobile banking. The use of the one-time password which is sent to the user mobile phone by short message service (SMS) is a vulnerability which we want to solve with proposing a new scheme called S-Mbank. We replace the authentication using the one-time password with the contactless smart card to prevent attackers to use the unencrypted message which is sent to the user's mobile phone. Moreover, it deals vulnerability of spoofer to send an SMS pretending as a bank's server. The contactless smart card is proposed because of its flexibility and security which easier to bring in our wallet than the common passcode generators. The replacement of SMS-based authentication with contactless smart card removes the vulnerability of unauthorized users to act as a legitimate user to exploit the mobile banking user's account. Besides that, we use public-private key pair and PIN to provide two factors authentication and mutual authentication. We use signcryption scheme to provide the efficiency of the computation. Pair based text authentication is also proposed for the login process as a solution to shoulder-surfing attack. We use Scyther tool to analyze the security of authentication protocol in S-Mbank scheme. From the proposed scheme, we are able to provide more security protection for mobile banking service.Comment: 6 page

MODEL SISTEM INFORMASI MANAJEMEN PRAKTIK KERJA LAPANGAN BERBASIS WEBSITE PADA PROGRAM STUDI MANAJEMEN PENDIDIKAN UNIVERSITAS NEGERI JAKARTA

Model Sistem Informasi Manajemen Praktik Kerja Lapangan pada Program Studi Manajemen Pendidikan Universitas Negeri Jakarta merupakan suatu terobosan ide pengembangan model sistem berbasis Website yang membantu Program Studi Manajemen Pendidikan dalam manajemen data dan informasi praktik kerja lapangan yang sebelumnya bersifat konvensional menjadi sistem digital yang efektif dan efisien. Sistem Informasi Manajemen Praktik Kerja Lapangan Berbasis Website pada Program Studi Manajemen Pendidikan Universitas Negeri Jakarta bertujuan membantu Tenaga Kependidikan, Mahasiswa, dan Pembimbing dalam merencanakan, mengelola dan melaporkan kegiatan Praktik Kerja Lapangan. Metode penelitian yang digunakan adalah Penelitian dan Pengembangan (Research and Devevelopment) Borg & Gall melalui sepuluh langkah yaitu: 1) penelitian pendahuluan, 2) perencanaan, 3) pengembangan produk, 4) uji coba lapangan tahap awal, 5) revisi produk tahap awal, 6) uji coba lapangan tahap akhir, 7) revisi produk tahap akhir, 8) uji coba operasional, 9) revisi produk final dan 10) implementasi. Hasil penelitian ini adalah terciptanya sebuah model sistem informasi manajemen praktik kerja lapangan berbasis website yang efektif dan efisien dengan tahapan perencanaan, pengelolaan, pelaporan, dan monitoring baik admin, mahasiswa maupun pembimbing Program Studi Manajemen Pendidikan; dan Hasil uji coba sistem menunjukan hasil yang memuasakan. Hal ini berdasarkan hasil uji coba produk tahap awal, uji coba produk tahap akhir hingga uji coba operasional lapangan. ***** The Website-Based Fieldwork Practice Management Information System Model for Department of Educational Management Universitas Negeri Jakarta is a breakthrough in the idea of developing a Website-based system model that helps the Department of Educational Management in data and information management of fieldwork practices that were previously conventional into an effective and efficient digital system. The Website-Based Fieldwork Practice Management Information System Model for Department of Educational Management Universitas Negeri Jakarta aims to assist Education Personnel, Students, and Supervisors in planning, managing and reporting fieldwork practice activities. The research method used is Research and Development (Research and Devevelopment) Borg & Gall through ten steps namely, namely: 1) preliminary research, 2) planning, 3) product development, 4) early-stage field trials, 5) early-stage product revision, 6) final field stage trials, 7) late-stage product revisions, 8) operational trials, 9) final product revisions and 10) implementation. The result of this study is the creation of an effective and efficient website-based fieldwork practice management information system model with the stages of planning, managing, reporting, and monitoring both admins, students and supervisors of the Education Management Study Program; and The results of system trials showed impressive results. This is based on the results of early-stage product trials, late-stage product trials to field operational trials

Analisis Pelaksanaan Pembelajaran Pemrosesan Informasi Dalam Mata Pembelajaran Sejarah Di Kelas XI Madrasah Aliyah Al-Muttaqin Kabupaten Kapuas Hulu

The purpose of this study was to determine the Implementation of Information Processing Learning in History Subjects in Class XI Madrasah Aliyah Al-Muttaqin, Kapuas Hulu Regency for the 2012/2013 academic year. based on a population of only 93 people, the entire population was taken as a sample so that this study was a population study. The data collection tools used were observation guides, questionnaires, interview guides, syllabus and lesson plans. Data processing and data analysis is known. Preparation of the implementation of information processing learning by history teachers is classified as very good with the results obtained by 85.7%. The implementation of information processing learning by history teachers is classified as very good with a result of 80.1%. Evaluation of the implementation of information processing learning by history teachers is classified as good with a result of 74.8. For this reason, information processing learning in history subjects needs to be continuously applied and developed in other materials so that the planned learning objectives can be achieved effectively

Protease from Courgette (Luffa Acutangula L (Roxb)): Isolation, Purification, and Some Characteristics

The Courgette or oyong (Luffa acutangula L. (Roxb)) is member of Cucurbitaceae mainly used as vegetable. Beside used as vegetables, courgette aslo used as keratolytic agent. This fact is supposed that this vegetables contain protease. This research is succeed to purified courgette’s protease by four step. That was precipitate by 70% ammonium sulphate saturation, purification using DEAE cellulose ion exchange chromatography and gel filtration chromatography using sephadex G-100 and G-75. Purified courgette’s protease had 81,922 U/mg for specific activity and 34 kDa molecular weight. This enzyme had the characteristic such as activated optimally at 37oC, pH 7 and 10 minute duration time. This enzyme activity can decrease by PMSF and H2O2, its remarkable that courgette protease is serine protease and had the thiol group in its structure. The ability to digest food proteins materials like boiled meat and boiled white egg by courgette protease proves that the courgette protease enzyme is could be used in enzyme replacement therapy in mild digestion problem.&nbsp

Characterization of Trypsin-Like Protease of Lactobacillus plantarum FNCC 0270

Trypsin is an enzyme that has a unique mechanism of cutting peptide bonds specifically at the carboxyl side of lysine or arginine amino acids, with another amino acid. This study aims to analyze a trypsin-like protease (TLP) found in Lactobacillus plantarum FNCC 0270,  by performing partial proteomic tests, i.e. MALDI-TOF/TOF, and standard bioinformatics tools. SDS-PAGE analysis showed 4 protein bands. Two bands of the (P1 and P2) showed molecular weights equivalent to 47.35 and 38.42 kD, each generating 8 and 11 peptide fragments respectively. According to information in www.ncbi.nlm.nih.gov/genbank/structures, the structure  of serine protease HtrA (subs. plantarum L. plantarum ST–III) consists of three domains. Using Clone Manager® software by aligning two sequences we obtained eleven. The Lactobacillus produces of the trypsin-like serine protease has 40-90% similarity. Using the Clustal W2 software we passed the 11 sequences through multiple alignments, and found that the isolate L. plantarum is closely related to L. buchneri, L. brevis, and L. malefermentans on the phylogenetic tree. Alignment analysis results showed that all 8 peptide fragments of band 1 and 11 peptide fragments of band 2,  of the SDS-PAGE, were located in the active domain region of  the fourth trypsin-like serine protease producing Lactobacilli

HDAC2 and PCNA expression is correlated to decreasing of endoxifen sensitivity in human breast cancer stem cells ALDH+

Latar belakang: Sel punca kanker payudara (breast cancer stem cells/BCSC) adalah subpopulasi sel kanker yang memiliki kemampuan menghasilkan tumor baru dan bersifat seperti sel punca. Penelitian kami sebelumnya menggunakan jaringan kanker payudara mengungkapkan bahwa ekspresi gen histone deacetylase 2 (HDAC2) dan proliferating cell nuclear antigen (PCNA) ditemukan perbedaan signifikan setelah terapi neoajuvan hormon dan kemoterapi. Penelitian ini bertujuan untuk menganalisis hubungan antara ekspresi HDAC2 dan PCNA dengan kelangsungan hidup sel punca kanker payudara dengan penanda aldehyde dehydrogenase + (ALDH+) yang diberi perlakuan endoksifen. Metode: Sampel adalah BCSC primer manusia ALDH+ yang diberi perlakuan endoksifen 4 uM masingmasing selama 2, 4, 6, 8, 10, 12, 14 hari. Viabilitas sel dilihat dengan menggunakan trypan blue dan ekspresi mRNA HDAC2 dan PCNA ditentukan menggunakan qRT-PCR. Hasil: Viabilitas BCSCs ALDH + menurun setelah 2 sampai 4 hari pemberian endoksifen. Pada periode ini juga didapatkan ekspresi mRNA HDAC2 dan PCNA mengalami penurunan. Tetapi setelah pemberian endoksifen selama 8 hari, viabilitas BCSCs ALDH + mengalami peningkatan dan ditemukan peningkatan yang signifikan pada hari ke-14 pemberian endoksifen. Ekspresi mRNA HDAC2 dan PCNA juga menunjukkan peningkatan mulai pada hari ke-8 dan terus meningkat hingga hari ke-14 pemberian endoksifen. Penelitian ini menunjukkan pola yang sama antara ekspresi mRNA HDAC2 dan PCNA dan viabilitas sel. Kesimpulan: Induksi endoksifen yang lama menurunkan sensitivitas efek endoksifen pada BCSC manusia dan ekspresi HDAC2 dan PCNA berkorelasi dengan viabilitas BCSC manusia setelah induksi endoksifen. (Health Science Journal of Indonesia 2019;10(2):77-81) Kata kunci: sel punca kanker payudara, viabilitas sel, HDAC2, PCNA, endoksifen   Abstract Background: Breast cancer stem cells (BCSCs) are subpopulation of cancer cells that has the ability to generate new tumor and similar properties to stem cell. Our previous study using breast cancer patients revealed that gene expression of histone deacetylase 2 (HDAC2) and proliferating cell nuclear antigen (PCNA) were significantly altered after neoadjuvant hormone and chemotherapy. This study aimed to analyze the correlation between HDAC2 and PCNA expressions with the viability of breast cancer stem cells aldehyde dehydrogenase + (BCSC ALDH+) treated by endoxifen. Method: Samples are human primary BCSCs ALDH+ that treated with 4 uM of endoxifen for 2, 4, 6, 8, 10, 12, 14 days, respectively. Cell viability was measured using trypan blue exclusion assay and the mRNA expressions of HDAC2 and PCNA were determined using qRT-PCR. Results: The viability of BCSCs ALDH+ was decreased after 2 days until 4 days-endoxifen treatment. It also demonstrated that mRNA expression of HDAC2 and PCNA were decreased in this period. But after 8 days endoxifen treatment, the viability of BCSCs ALDH+ was increased. The increasing of viability was higher in 14 days-endoxifen treatment. The mRNA expression of HDAC2 and PCNA also showed increasing begin on 8 days and continued to increase until 14-days endoxifen treatment. We found a similar pattern between HDAC2 and PCNA expression and cell viability. Conclusion: Prolonge endoxifen treatment decrease sensitivity of endoxifen effect in human BCSC and the expression of HDAC2 and PCNA are correlated to human BCSCs viability after endoxifen treatment. (Health Science Journal of Indonesia 2019;10(2):77-81) Keywords: human breast cancer stem cells, viability, HDAC2, PCNA, endoxife

Oxidative Stress, Hypoxia-Inducible Factor-1α, and Nuclear Factor-Erythroid 2-Related Factor 2 in the Hearts of Rats Exposed to Intermittent Hypobaric Hypoxia

Hypobaric hypoxia is situation that might occur to helicopter pilots in Indonesia who must fly at an altitude of more than 3,048 m such as in Papua. It can be dangerous because hypoxic condition can affect person's performance. So far, the heart is known as an aerobic organ and very sensitive to hypoxic conditions. Hitherto, the effects of hypobaric hypoxia exposure on biomolecular aspects of the heart are still unclear. Therefore, this study assessed cardiac response in rats exposed to intermittent hypobaric hypoxia (IHH) (equivalent to 3,048 meters/10,000 feet). Sprague-Dawley rats were divided into six groups: control; acute hypobaric hypoxia (AHH); and IHH, for 7; 14; 21; and 28 days. We measured super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH), malondialdehyde (MDA), cytoglobin, myoglobin, HIF-1α, and Nrf2 level as our parameters. Activities of SOD, CAT, GPx and GSH increased while the levels of MDA, cytoglobin, myoglobin, HIF-1α, and Nrf2 decreased in all IHH groups compared with the AHH group. A biphasic pattern was observed as IHH sessions increased from 14 to 21 or 28. Where the IHH treatment for more than 14 sessions caused a decrease in endogenous antioxidants, but the response to hypoxia and oxidative stress increased. Our findings presented the molecular alterations of cardiac rats exposed to intermittent hypobaric hypoxia

RESPONSES OF BRAIN TISSUES AGAINST HYPOXIC CONDITION IN HEMORRHAGIC STROKE PATIENTS: NEUROGLOBIN EXPRESSION IN BRAIN TISSUE AND PLASMA

Objective: Strokes remain a significant health concern and are the highest cause of mortality and physical or mental disability in productive and the elderly hospitalized patients in Indonesia. Neuroglobin (Ngb) mostly located in the central and peripheral nervous system, predicted enhanced neuronal survival under hypoxic condition, such as in a stroke. The aim of this study is to observe the response of the brain tissue of hemorrhagic stroke patients against hypoxic/ischemic conditions. The objectives are to recognize the pattern of Ngb expression in the brain tissue and plasma of hemorrhagic stroke patients, and furthermore, to compare the level of Ngb in the brain tissue and plasma of hemorrhagic stroke patients.Methods: This is an observational study with consecutive sampling methods using cerebral cortex and the blood of hemorrhagic stroke patients, who underwent craniotomies to evacuate hematomas at Cipto Mangunkusumo Hospital (RSCM) and other hospitals in Jakarta. Ngb expression was measured in brain tissue and blood using real time reverse transcription polymerase chain reaction, while the ELISA method was adopted to measure Ngb protein in plasma.Results: Hypoxia/ischemia in the brain tissue of hemorrhagic stroke patients increased the expression of Ngb in brain tissue compared to the blood. The level of Ngb protein in plasma of hemorrhagic stroke patients increased significantly compared to normal subjects; however, there is no significant difference between the plasma and brain tissue of hemorrhagic stroke patients.Conclusion: Hypoxia/ischemia in hemorrhagic stroke patients increases the expression of Ngb mRNA and protein level.Keywords: Neuroglobin, Hypoxia, Hemorrhagic stroke