Accelerated HIV testing for PMTCT in maternity and labour wards is vital to capture mothers at a critical point in the programme at district level

TORONTO AIDS Conference 200

Differences in time until dispersal between cryptic species of a marine nematode species complex

Co-occurrence of closely related species may be achieved in environments with fluctuating dynamics, where competitively inferior species can avoid competition through dispersal. Here we present an experiment in which we compared active dispersal abilities (time until first dispersal, number and gender of dispersive adults, and nematode densities at time of dispersal) in Litoditis marina, a common bacterivorous nematode species complex comprising four often co-occurring cryptic species, Pm I, II, III, and IV, as a function of salinity and food distribution. The experiment was conducted in microcosms consisting of an inoculation plate, connection tube, and dispersal plate. Results show species-specific dispersal abilities with Pm I dispersing almost one week later than Pm III. The number of dispersive adults at time of first dispersal was species-specific, with one dispersive female in Pm I and Pm III and a higher, gender-balanced, number in Pm II and Pm IV. Food distribution affected dispersal: in absence of food in the inoculation plate, all species dispersed after ca four days. When food was available Pm I dispersed later, and at the same time and densities irrespective of food conditions in the dispersal plate (food vs no food), suggesting density-dependent dispersal. Pm III dispersed faster and at a lower population density. Salinity affected dispersal, with slower dispersal at higher salinity. These results suggest that active dispersal in Litoditis marina is common, density-dependent, and with species, gender- and environment-specific dispersal abilities. These differences can lead to differential responses under suboptimal conditions and may help to explain temporary coexistence at local scales

To stay or go: differential dispersal rates in cryptic species of a marine nematode

Behind the morphological similarity of many species, a hidden genetic diversity can be found. This cryptic diversity has been well documented in the marine nematode Rhabditis (Pellioditis) marina, a common bactivore associated with decomposing macro-algae in the littoral zone of coastal and estuarine environments. Four cryptic species of R. marina (Pm I, Pm II, Pm III and Pm IV) co-occur along the south-western coast and estuaries of The Netherlands. This coexistence challenges traditional competition theory, which states that competition will be most severe between closely related species. A previous study showed that competition between the four cryptic species occurred, but interspecific interactions were affected by environmental conditions such as salinity. One of the most important mechanisms to react to competition is dispersal. Most organisms have at least one stage in which dispersal occurs over a specific spatial scale. Meiobenthic species, however, lack any pelagic stage. But, recent research shows more and more evidence that meiofauna is also able to actively disperse with lateral sinusoidal movements in the interstitial spaces as one of the most common modes of dispersal of nematodes over short distances. Dispersal is a process triggered partially by the internal conditions of organisms and partially by environmental conditions. In the current experiment, species-specific differences in active dispersal rates and the effects of salinity and food distribution on dispersal behaviour were tested in four cryptic species of R. marina (Pm I, Pm II, Pm III and Pm IV), The results of the experiment showed that dispersal is a species-specific behaviour with Pm III being the most rapid disperser (first dispersal event occurred after an average of 3 days), and Pm I the slowest disperser (average of 10 days). An effect of food distribution on the dispersal rates of all cryptic species was found with the most rapid dispersal if no food was present at the start situation. Salinity also had an effect with a higher dispersal rate at lower salinity for all the species. Moreover, the number of dispersive organisms differed between the species, with only one female disperser in Pm I and Pm III and a mix of female and male dispersers for Pm II and Pm IV at the first dispersal event. This species-specific dispersal behaviour and the influence of external conditions on dispersal can influence the reaction on competition. In a future experiment the effect of competition on dispersal will be studied by using competition cultures, where all four cryptic species can influence each other, but the organisms have the chance to disperse away. These results are important to better understand the processes behind the coexistence of cryptic species

Journal Staff

Primary Immunodeficiencies (PID) are genetically inherited disorders characterized by defects of the immune system, leading to increased susceptibility to infection. Due to the variety of clinical symptoms and the complexity of current diagnostic procedures, accurate diagnosis of PID is often difficult in daily clinical practice. Thanks to the advent of "next generation'' sequencing technologies and target enrichment methods, the development of multiplex diagnostic assays is now possible. In this study, we applied a selector-based target enrichment assay to detect disease-causing mutations in 179 known PID genes. The usefulness of this assay for molecular diagnosis of PID was investigated by sequencing DNA from 33 patients, 18 of which had at least one known causal mutation at the onset of the experiment. We were able to identify the disease causing mutations in 60% of the investigated patients, indicating that the majority of PID cases could be resolved using a targeted sequencing approach. Causal mutations identified in the unknown patient samples were located in STAT3, IGLL1, RNF168 and PGM3. Based on our results, we propose a stepwise approach for PID diagnostics, involving targeted resequencing, followed by whole transcriptome and/or whole genome sequencing if causative variants are not found in the targeted exons

Meta-Analysis of Neurobehavioral Outcomes in Very Preterm and/or Very Low Birth Weight Children

OBJECTIVE: Sequelae of academic underachievement, behavioral problems, and poor executive function (EF) have been extensively reported for very preterm (≤33 weeks' gestation) and/or very low birth weight (VLBW) (≤1500 g) children. Great variability in the published results, however, hinders the field in studying underlying dysfunctionsanddeveloping intervention strategies. We conductedaquantitative meta-analysis of studies publishedbetween1998and 2008 on academic achievement, behavioral functioning, and EF with the aim of providing aggregated measures of effect size for these outcome domains. METHODS: Suitable for inclusion were 14 studies on academic achievement, 9 studies on behavioral problems, and 12 studies on EF, which compared a total of 4125 very preterm and/or VLBW children with 3197 term-born controls. Combined effect sizes for the 3 outcome domains were calculated in terms of Cohen's d. Q-test statistics were performed to test homogeneity among the obtained effect sizes. Pearson's correlation coefficients were calculated to examine the impact of mean birth weight and mean gestational age, as well as the influence of mean age at assessment on the effect sizes for academic achievement, behavioral problems, and EF. RESULTS: Combined effect sizes show that very preterm and/or VLBW children score 0.60 SD lower on mathematics tests, 0.48 SD on reading tests, and 0.76 SD on spelling tests than term-born peers. Of all behavioral problems stacked, attention problems were most pronounced in very preterm and/or VLBW children, with teacher and parent ratings being 0.43 to 0.59 SD higher than for controls, respectively. Combined effect sizes for parent and teacher ratings of internalizing behavior problems were small ( 0.51). CONCLUSIONS: Very preterm and/or VLBW children have moderate-toseveredeficits inacademicachievement,attentionproblems, andinternalizing behavioral problems and poor EF, which are adverse outcomes that were strongly correlated to their immaturity at birth. During transition to young adulthood these children continue to lag behind term-born peers

Black Dialect in Children\u27s Books

Black non-Standard English is different in grammar (syntax) from Standard English. The advent of the 60\u27s produced authors who explored the full possibilities of language to deal with their themes. The increased use of dialect by black authors, particularly children\u27s authors, was a sign that the nature of the black experience as they wanted to convey it did not have to rely on traditional forms, and literary devices; that they could treat familiar, realistic ideas and situations using a familiar dialect and relate that idea more effectively