Master of Science

thesisRecent neurobiological, epidemiological, and psychological research has provided evidence for a dissociative subtype of PTSD. However, much of the current evidence base has only included samples of adults with acute trauma exposure, and the literature has not yet determined if there are systematic differences between individuals who do and do not meet criteria for the dissociative subtype. Therefore, additional research is needed to determine whether the dissociative subtype is valid in youth with chronic trauma exposure and what factors affect the likelihood of dissociative subtype membership in youth. Using a sample of 248 adolescents (90 girls, 158 boys) between the ages of 13 to 19, this study sought to confirm the dissociative subtype of PTSD in a sample of traumatized adolescents and to investigate whether peritraumatic dissociation, emotion dysregulation, gender, and betrayal trauma exposure affected the likelihood of dissociative subtype membership. Results indicated that approximately half of participants (50.8%) met criteria for the dissociative subtype. Furthermore, results of logistic regression indicated that adolescents with greater levels of peritraumatic dissociation and emotion dysregulation were at an increased risk of subtype membership. This study has important implications concerning those adolescents who may be particularly likely to receive the dissociative subtype label

Comparing Traumatic Symptoms at Home and in Therapy for Preschoolers Exposed to Intimate Partner Violence (IPV)

Psychologyhttp://deepblue.lib.umich.edu/bitstream/2027.42/85305/1/crosbymo.pd

Polyvictimization, Emotion Dysregulation, Symptoms of Posttraumatic Stress Disorder, and Behavioral Health Problems among Justice-Involved Youth: a Latent Class Analysis

Among the 90% of adolescents involved in juvenile justice who have experienced traumatic victimization, a sub-group may be at highest risk due to histories of multiple types of interpersonal and non-interpersonal trauma, termed polyvictims. Latent class analyses (LCA) have identified polyvictimized subgroups in several studies of adolescents and adults, but only one study of traumatic victimization has been conducted with justice-involved youth (Ford et al. 2013). The current investigation replicates and extends that study’s findings using LCA to assess a wider range of victimization- and nonvictimization-related adversities and emotion dysregulation, DSM-5 symptom clusters of posttraumatic stress disorder (PTSD), and behavioral health problems, such as substance use, anger, depression, somatic complaints, and suicide ideation. In a sample of juvenile detainees three latent classes were identified: mixed adversity (MA; n = 327), violent environment (VE; n = 337), and polyvictimization (PV; n = 145). In contrast to MA youth, PV youth were more likely to report exposure to all forms of adversity, and in contrast to both MA and VE youth, exposure to maltreatment and family violence, and higher levels of emotion dysregulation, PTSD, and depression/anxiety symptoms, somatic complaints, and suicidality. VE youth (vs. MA youth) were more likely to report exposure to violence and non-interpersonal traumas, and were higher on some forms of emotion dysregulation, PTSD symptoms, anger and substance use. Findings suggest that most justice-involved youth have experienced substantial adversity, with almost one in five identified as a polyvictim having experienced multiple adversities, including impaired caregivers, and evidencing the most severe problems in emotion dysregulation and PTSD, internalizing, and externalizing symptoms

Glial cell line-derived neurotrophic factor influences proliferation of osteoblastic cells

Little is known about the role of neurotrophic growth factors in bone metabolism. This study investigated the short-term effects of glial cell line-derived neurotrophic factor (GDNF) on calvarial-derived MC3T3-E1 osteoblasts. MC3T3-E1 expressed GDNF as well as its canonical receptors, GFRα1 and RET. Addition of recombinant GDNF to cultures in serum-containing medium modestly inhibited cell growth at high concentrations; however, under serum-free culture conditions GDNF dose-dependently increased cell proliferation. GDNF effects on cell growth were inversely correlated with its effect on alkaline phosphatase (ALP) activity showing a significant dose-dependent inhibition of relative ALP activity with increasing concentrations of GDNF in serum-free culture medium. Live/dead and lactate dehydrogenase assays demonstrated GDNF did not significantly affect cell death or survival under serum-containing and serum-free conditions. The effect of GDNF on cell growth was abolished in the presence of inhibitors to GFR α 1 and RET indicating that GDNF stimulated calvarial osteoblasts via its canonical receptors. Finally, this study found that GDNF synergistically increased tumor necrosis factor-α (TNF-α)-stimulated MC3T3-E1 cell growth suggesting that GDNF interacted with TNF-α-induced signaling in osteoblastic cells. In conclusion, this study provides evidence for a direct, receptor-mediated effect of GDNF on osteoblasts highlighting a novel role for GDNF in bone physiology. \ud \u

Syndecan-1 Enhances Proliferation, Migration and Metastasis of HT-1080 Cells in Cooperation with Syndecan-2

Syndecans are transmembrane heparan sulphate proteoglycans. Their role in the development of the malignant phenotype is ambiguous and depends upon the particular type of cancer. Nevertheless, syndecans are promising targets in cancer therapy, and it is important to elucidate the mechanisms controlling their various cellular effects. According to earlier studies, both syndecan-1 and syndecan-2 promote malignancy of HT-1080 human fibrosarcoma cells, by increasing the proliferation rate and the metastatic potential and migratory ability, respectively. To better understand their tumour promoter role in this cell line, syndecan expression levels were modulated in HT-1080 cells and the growth rate, chemotaxis and invasion capacity were studied. For in vivo testing, syndecan-1 overexpressing cells were also inoculated into mice. Overexpression of full length or truncated syndecan-1 lacking the entire ectodomain but containing the four juxtamembrane amino acids promoted proliferation and chemotaxis. These effects were accompanied by a marked increase in syndecan-2 protein expression. The pro-migratory and pro-proliferative effects of truncated syndecan-1 were not observable when syndecan-2 was silenced. Antisense silencing of syndecan-2, but not that of syndecan-1, inhibited cell migration. In vivo, both full length and truncated syndecan-1 increased tumour growth and metastatic rate. Based on our in vitro results, we conclude that the tumour promoter role of syndecan-1 observed in HT-1080 cells is independent of its ectodomain; however, in vivo the presence of the ectodomain further increases tumour proliferation. The enhanced migratory ability induced by syndecan-1 overexpression is mediated by syndecan-2. Overexpression of syndecan-1 also leads to activation of IGF1R and increased expression of Ets-1. These changes were not evident when syndecan-2 was overexpressed. These findings suggest the involvement of IGF1R and Ets-1 in the induction of syndecan-2 synthesis and stimulation of proliferation by syndecan-1. This is the first report demonstrating that syndecan-1 enhances malignancy of a mesenchymal tumour cell line, via induction of syndecan-2 expression

Doctor of Philosophy

dissertationPrevious research has indicated that youth with a history of maltreatment are more likely to evidence offending behaviors and experience formal involvement in the juvenile justice system compared to nonmaltreated youth. In recent years, increased efforts have been made towards identifying the specific characteristics and correlates of youth known to both the child welfare and juvenile justice systems, a population often referred to as "crossover youth." However, despite identifying risk factors associated with the crossover population, there are a number of limitations to the extant literature, many of which stem from the fact that the majority of studies involving crossover youth thus far have been descriptive in nature. This study investigated the influence of theoretically- and developmentally-relevant constructs, including attachment- and nonattachment-related trauma, emotion dysregulation, posttraumatic risk-seeking (PTRS), and other posttraumatic stress symptoms (PTSS), in the trajectory from maltreatment exposure to committing offending behaviors. Specifically, the current study investigated the direct and indirect associations among attachment- and nonattachment-related trauma, emotion dysregulation, PTSS, PTRS, and offending behavior, and whether these relations were moderated by gender or by system status. Additionally, the study investigated the unique contribution of PTRS in predicting offending behavior. Results demonstrated a number of significant differences in means, direct, and indirect effects between crossover and JJ-only youth and boys and girls. Additionally, results of hierarchical Poisson regression demonstrated that PTRS did account for significantly more variance in offending behavior when offending behavior was measured via self-report. Finally, results of mixture modeling identified two distinct risk-seeking groups, and, as predicted, high risk-seekers evidenced significantly greater levels of attachment-related trauma exposure, emotion dysregulation, and PTSS. This study has clinical, research, and theoretical implications for better understanding the risk factors and mechanisms associated with the crossover trajectory

La calpaïne - 6 (une nouvelle cible thérapeutique dans les ostéosarcomes)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Régulation du syndécan-2 dans l'ostéosarcome (interaction avec l'action des agents cytotoxiques)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

The development of a fully automated procedure to produce a technology transfer directory of people to facilitate the linker function in the technology transfer process

http://archive.org/details/developmentofful00mod