Cortical modulation of acute post-thoracotomy pain with transcranial direct current stimulation

Transkranijalna stimulacija jednosmernom strujom (tSJS) se primenjuje u terapiji različitih hroničnih bolnih stanja i u terapiji akutnog postoperativnog bola za određene tipove procedura i hirurških zahvata (endoskopska retrogradna holangiopankreatografija, hirurški zahvati na nivou lumbalne kičme, totalne artroplastike kolena i hirurškog rešavanja hallux valgus-a). Torakotomija se karakteriše visokim intenzitetom bola, a njegovo kompleksno poreklo zahteva primenu multimodalnog analgetskog režima. Uzevši u obzir neželjene efekte farmakoloških agenasa koji se primenjuju u terapiji bola, neophodna su ispitivanja efekata novih nefarmakoloških metoda. Transkranijalna stimulacija jednosmernom strujom je tehnika neinvazivne kortikalne stimulacije sa neuromodulatornim efektima, koja menja kortikalnu ekcitabilnost modulacijom ispod praga nadražljivosti membranskog potencijala neurona u mirovanju putem konstantne slabe električne struje. Prednosti primene tSJS u svakodnevnoj kliničkoj praksi su bezbednost pacijenta, jednostavnost primene i izostanak remećenja funkcionisanja ostalih medicinskih aparata

Tolerance of autochthonous lactic acid bacteria to different processing conditions in vitro

In this paper, the effect of different temperatures, pH, and NaCl concentration on the growth of autochthonous lactic acid bacteria isolated from traditionally made Serbian cheese (Sokobanja area) was investigated by using the spectrophotometric method. Growth of tested Lactobacillus (Lb. fermentum, Lb. plantarum, and Lb. brevis) and Lactococcus lactis subsp. lactis biovar. diacetylactis five isolates were better in acidic pH, while the growth of Enterococcus isolates (E. durans, E. faecium, and E. faecalis) was better in basic pH, at 37 °C. At 4 °C after 24 h, none of the tested bacteria showed growth. Since the autochthonous isolates were tolerant to a tested range of dairy processing conditions, further studies need to include the characterization of enzymatic activity of selected isolates, as well as the ability to use these isolates like starter cultures or food supplements in dairy or non-dairy products.Accepted for publishin

Effect of Different Types of Fly Ash on Properties of Asphalt Mixtures

In order to preserve natural resources, the use of waste and alternative materials in the construction and maintenance of roads is increasingly investigated. This paper presents the results of testing wearing course asphalt mixtures (AC 11s SURF 50/70) made with various percentages of fly ash, used as a partial or complete substitute for mineral filler. The properties of fly ash were determined to assess their suitability for use in asphalt mixtures. The experimental research was performed on asphalt samples containing fly ash from three different sources, with 25%, 50%, 75%, and 100% of mineral filler substitution. The control mixture was prepared with 100% of mineral filler. The paper presents the volumetric composition, stability, and flow of asphalt mixtures tested on standard Marshall's samples, water sensitivity, and resistance to permanent deformation. The results of this study indicate that a satisfactory volumetric composition can be achieved by adding fly ash, while the bulk density and voids of the mineral and asphalt mixture generally depend on the type of fly ash and its content. The stability and flow of mixtures with fly ash are favourable compared with the control mixture. The water sensitivity of mixtures with fly ash is generally lower compared with the control mixture and depends on the type and percentage of fly ash. The resistance to permanent deformation of the asphalt mixtures depends on the fly ash type and percentage. The results obtained in this study are an important step towards broader implementation of fly ash in asphalt mixtures

Contribution to the knowledge about the presence and role of Enterobacter gergoviae in sensory characteristics of dairy products

Enterobacter gergoviae KGPMF 20 was found in traditionally made cheese from Sokobanja (South-eastern Serbia). In this paper, the characteristics of the species were evaluated by investigation of adhesion to different solvents and co-aggregation ability with other species. Moreover, its enzymatic activity was evaluated by using spectrophotometric method, with the intention to detect the role of the isolate in the sensory characteristic of cheese. The results of enzymatic activity indicated that E. gergoviae KGPMF 20 has low, almost no enzymatic activity. It could be concluded that this isolate did not affect the sensory characteristic of cheese.Publishe

The Late Antique dietary habits in Western Serbia: a case study of the Čačak – Dvorište Gimnazije site

The analysed faunal material was collected during the rescue archaeological campaigns conducted between 2014 and 2018 by the National Museum Čačak at the Dvorište Gimnazije site in Čačak (Western Serbia). The Late Antique building remains, constructed in the 3rd–4th century, preserved at the foundation level were discovered at the site. After its destruction, building was reused during the late 4th and the 5th century but in a more primitive manner. Remains of a few pits, hearths, and probably primitive dwellings belong to this horizon. The 3rd horizon at this site dates to the Late Medieval period (the 15th and the first half of the 16th century), and it is represented by a Christian necropolis dug into the Roman building. Faunal material is more abundant in the Late Antique period than in the Late Medieval context. The remains of mammals form the majority of the faunal sample. Domestic species are more frequent within the assemblage and include cattle, pigs, sheep and goats, horses, dogs, and cats. Furthermore, wild species are represented by wild boar, red deer, roe deer, fox, and hare. The most common are the remains of cattle, followed by pigs and caprines. Among wild mammals, the most numerous remains belong to wild boar and red deer. The greater importance of domestic mammals is expected for the Late Antique and Medieval archaeological sites in Serbia. In addition, the bird remains were confirmed as well. Among them, the remains of chicken were the most numerous. Since this is a newly discovered site, additional archaeological excavations in the vicinity are needed to create a broader picture of this site and its role within the currently unnamed ancient settlement that lasted throughout the centuries by the Western Morava River. Moreover, new zooarchaeological research will enable a better understanding of human–animal relationships of the inhabitants of this settlement in the past

Species diversity of phytophagous and predatory mites of wild fruit trees in forest ecosystems of Serbia

Proučavanjem fitofagnih i predatorskih grinja samoniklih vrsta voćaka u šumskim ekosistemima Srbije u periodu 2000-2013 utvrđeno je prisustvo: - 37 vrsta iz natfamilije Eriophyoidea od kojih jedna vrsta pripada familiji Phytoptidae, 31 vrsta familiji Eriophyidae i pet vrsta familiji Diptilomiopidae, svrstanih u 18 rodova. Za faunu Srbije novih je 14 vrsta eriofida. Njihovo prisustvo ustanovljeno je na 41 istraživanom lokalitetu na 21 vrsti biljke hraniteljke. Za tri vrste Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. - 20 vrsta iz familije Tetranychidae, od toga šest vrsta pripada potfamiliji Bryobiinae a14 potfamiliji Tetranychinae, grupisanih u osam rodova. Za faunu Srbije zabeleženo je sedam novih vrsta paučinara iz dva roda. Za 13 vrsta paučinara zabeležene su nove biljke hraniteljke. Prikazani su rezultati sa 39 lokaliteta i 19 biljnih vrsta hraniteljki. - 26 vrsta iz familije Phytoseiidae. Potfamilija Amblyseiinae obuhvata osam vrsta, potfamilija Phytoseiinae šest vrsta i potfamilija Typhlodrominae 12 vrsta iz ukupno 11 rodova. Za faunu Srbije novih je 12 vrsta fitozeida. Vrste Phytoseius corniger i Typhloseiulus arzakanicus su prvi put zabeležene u fauni Evrope. Za vrstu Typhloseiulus arzakanicus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. Prikazani su rezultati sa 44 lokaliteta i 23 biljne vrste. Za sve tri grupe grinja dati su podaci o distribuciji nađenih vrsta u svetu i u Srbiji, odnos prema biljci hraniteljki i moguća štetnost ili korist. Za svaku vrstu dat je taksonomski validni status i tipski podaci, naveden je pun naziv i sinonimi, ukoliko postoje, kao i najznačajnije karakteristike...By studying phytophagous and predatory mites on wild fruit species of forest ecosystems of Serbia in the period 2000-2013 it was determined the presence of: - 37 species from the superfamily Eriophyoidea of which 1 species belongs to the family Phytoptidae, 31 species belong to the family Eriophyidae and 5 species belong to the family Diptilomiopidae grouped into 18 genera. There are 14 new species of eriophyoid mites for fauna of Serbia. Their presence was determined at 41 studying localities on 21 host plants. For 3 species Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Serbia is the second locality after the type locality. - 20 species from the family Tetranychidae of which 6 species belong to the subfamily Bryobiinae and 14 species to the subfamily Tetranychinae that were grouped into 8 genera. 7 new species of spider mites from 2 genera were recorded for the fauna of Serbia. The new host plants were determined for 13 species. The results from 39 localities and 19 host plants are presented. - 26 species from the family Phytoseiidae include 8 species of subfamily Amblyseiinae, 6 species of subfamily Phytoseiinae and 12 species of subfamily Typhlodrominae from a total of 11 genera. For our country 12 species of phytoseiid mites were recorded for the first time. For the fauna of Europe 2 species Phytoseius corniger and Typhloseiulus arzakanicus were recorded for the first time. For one species Typhloseiulus arzakanicus Serbia is the second locality after the type locality. The results from 44 localities and 23 host plants are presented. Data on the distribution of species found in the world and in Serbia, the relationship with the host plant and the possible harm and benefit were given for all three groups of mites..

Uticaj elektrofilterskog pepela na karakteristike asfaltnih mješavina

U radu su prikazani rezultati ispitivanja asfaltnih mješavina AB 11s spravljenih sa različitim procentima elektrofilterskog pepela, pri čemu je pepeo korišten kao djelimična ili potpuna zamjena za kameno brašno filer. U radu su predstavljeni rezultati ispitivanja svojstava elektrofilterskih pepela kako bi se utvrdila njihova podobnost za primjenu u asfaltnim mjršavinama. Eksperiment je izveden na asfaltnim uzorcima spravljenim sa pepelima iz tri različita izvora, a procenti u kojima je vršena zamjena kamenog brašna su 25%, 50%, 75% i 100%, dok je kontrolna mješavina spravljena sa 100% kamenog brašna. U radu je prikazana zapreminska struktura mješavina korištenih u eksperimentu. Izvedeni zaključci ukazuju da se dodavanjem elektrofilterskog pepela može postići zadovoljavajuća zapreminska struktura, pri čemu zapreminska masa i šupljine u mineralnoj i asfaltnoj mješavini generalno zavise od vrste pepela i njegovog sadržaja. Stabilnost i tečenje mješavina sa elektrofilterskim pepelom su poboljšani u odnosu na kontrolnu mješavinu. Sa povećanjem udjela elektrofilterskog pepela povećava se otpornost asfaltne mješavine na trajnu deformaciju. Osjetljivost uzoraka na vodu je uglavnom manja kod mješavina sa pepelom u odnosu na kontrolnu mješavinu što zavisi od vrste i količine pepela

Uticaj elektrofilterskog pepela na karakteristike asfaltnih mješavina

U radu su prikazani rezultati ispitivanja asfaltnih mješavina AB 11s spravljenih sa različitim procentima elektrofilterskog pepela, pri čemu je pepeo korišten kao djelimična ili potpuna zamjena za kameno brašno filer. U radu su predstavljeni rezultati ispitivanja svojstava elektrofilterskih pepela kako bi se utvrdila njihova podobnost za primjenu u asfaltnim mjršavinama. Eksperiment je izveden na asfaltnim uzorcima spravljenim sa pepelima iz tri različita izvora, a procenti u kojima je vršena zamjena kamenog brašna su 25%, 50%, 75% i 100%, dok je kontrolna mješavina spravljena sa 100% kamenog brašna. U radu je prikazana zapreminska struktura mješavina korištenih u eksperimentu. Izvedeni zaključci ukazuju da se dodavanjem elektrofilterskog pepela može postići zadovoljavajuća zapreminska struktura, pri čemu zapreminska masa i šupljine u mineralnoj i asfaltnoj mješavini generalno zavise od vrste pepela i njegovog sadržaja. Stabilnost i tečenje mješavina sa elektrofilterskim pepelom su poboljšani u odnosu na kontrolnu mješavinu. Sa povećanjem udjela elektrofilterskog pepela povećava se otpornost asfaltne mješavine na trajnu deformaciju. Osjetljivost uzoraka na vodu je uglavnom manja kod mješavina sa pepelom u odnosu na kontrolnu mješavinu što zavisi od vrste i količine pepela

Escherichia coli biofilm formation and control by phenolic compounds from Salvia officinalis L. extracts

Biofilm is recognized as one of the virulence factors of uropathogenic Escherichia coli. It offers bacteria significantly increased tolerance to antibiotics and makes difficulties in infection eradication. In this study, the biofilm formation ability of clinical isolates of uropathogenic E. coli as well as the antibiofilm activity of the common Garden sage, Salvia officinalis L. extracts were investigated in vitro. The influence of growth conditions (nutrient composition and incubation period) on biofilm formation of E. coli strains was evaluated using crystal violet staining procedure. The tested strains better formed biofilms after a longer incubation period (48 h). In addition, biofilm formation depended on nutrient medium composition. The eight strains were slime producers (Congo red agar assay). The water, ethanol, acetone and diethyl ether S. officinalis extracts were screened for their ability to inhibit the formation and metabolic activity of E. coli biofilms using crystal violet and resazurin assay, respectively. The water extract was not active, while ethanol, acetone and diethyl ether extract exhibited antibiofilm activity (MBIC= 2.5-10 mg/mL). The microscopic visualization of treated E. coli LM1 biofilm has shown morphological and density changes. In addition, the concentrations of phenolic compounds were determined spectrophotometrically. The highest content of total phenolics was found in acetone extract while the flavonoids in water extract.

In vitro interaction between Agrimonia eupatoria L. extracts and antibiotic

Synergistic activity between water, acetone, ethanol and diethyl ether extract of Agrimonia eupatoria L. and commonly used antibiotic (ampicillin) were evaluated. Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa were used. Interaction between plant extracts and antibiotic were tested by checkerboard method and expressed as fractional inhibitory concentration (FIC) index showed indifferent, additive and synergistic effects. Synergism was observed against E. coli for every combination of agents. FICI values were ranged from 0.03 to 0.29. Inhibitory concentration (IC50) was evaluated for every combination of tested extracts and antibiotic and the best combinations for every tested bacteria were combination of diethyl ether extract + ampicillin and combination of acetone extract + ampicillin