Induction of expressions of HLA class I and class II antigens by interferon on human renal cell carcinoma or bladder cancer cell lines

Interferon-induced expressions of HLA class I and class II (DR, DP, DQ) antigens on human renal cell carcinoma (RCC) cell lines and bladder cancer cell lines were analyzed by flowcytometry. Class I antigen was present on all six different RCC cell lines and the expression was enhanced with IFN-γ treatment except one cell line, Caki-2. Although no RCC cell lines expressed class II antigens without IFN-γ treatment, the treatment induced the expression of DR antigen in all cell lines except Caki-2. DP antigen was induced on KPK-1 and KPK-13 with exposure to IFN-γ. On the other hand, bladder cancer cell lines studied showed surface expression of class I antigen and enhanced expression of class I antigen with IFN-γ treatment. However, IFN-γ didn't enhance the expression of class II antigens on any of those bladder cancer cell lines. The minimum concentrations of IFN-γ to increase the expression of class I and DR andtigens on both KPK-13 and ACHN were 4 IU/ml and 100 IU/ml, respectively. The incubation time with IFN-γ to get the maximal expression of class I and DR antigens on KPK-13 were 36 hr and 48 hr, respectively. Meanwhile, although the expression of class I antigen on RCC cell lines was enhanced by IFN-α, IFN-α could not give any effect in class II antigens. Our results demonstrate the obvious effect of IFN-γ to enhance the expression of not only HLA class I antigen but also class II antigens on RCC cell lines

腎クリーゼを起こした強皮症の1例

発症より10年後腎クリーゼにて急性腎不全を起こした進行性全身性硬化症の1例を経験した.急激な腎機能低下, コントロール不良な高血圧, 大量の消化管出血を認め, 治療開始約1カ月後死亡した.本症例では入院時すでにDICと腎不全が高度であったこと, 消化管出血によりアンギオテンシン変換酵素阻害剤であるenalaprilが経口投与できなかったことが予後不良であった原因と考えられた.剖検時の腎の光顕所見で動脈内膜の肥厚と軽度の腎硬化像が認められた.We report a case of progressive systemic sclerosis with scleroderma renal crisis 10 years after onset. The patient (female) had progressive renal dysfunction, hypertension which was difficult to control, and massive gastrointestinal bleeding. An angiotensin converting enzyme inhibitor (enalapril) could not control her hypertension. Only intravenous nicardipine had a slight effect on her hypertension. Hemodialysis and plasma exchange, did not reverse the renal crisis, and the patient died. Microscopic examination of her kidney showed thickening of the capillary walls and mild nephrosclerosis