Tenascin-C fragments are endogenous inducers of cartilage matrix degradation

Cartilage destruction is a hallmark of osteoarthritis (OA) and is characterized by increased protease activity resulting in the degradation of critical extracellular matrix (ECM) proteins essential for maintaining cartilage integrity. Tenascin-C (TN-C) is an ECM glycoprotein, and its expression is upregulated in OA cartilage. We aimed to investigate the presence of TN-C fragments in arthritic cartilage and establish whether they promote cartilage degradation. Expression of TN-C and its fragments was evaluated in cartilage from subjects undergoing joint replacement surgery for OA and RA compared with normal subjects by western blotting. The localization of TN-C in arthritic cartilage was also established by immunohistochemistry. Recombinant TN-C fragments were then tested to evaluate which regions of TN-C are responsible for cartilage-degrading activity in an ex vivo cartilage explant assay measuring glycosaminoglycan (GAG) release, aggrecanase and matrix metalloproteinase (MMP) activity. We found that specific TN-C fragments are highly upregulated in arthritic cartilage. Recombinant TN-C fragments containing the same regions as those identified from OA cartilage mediate cartilage degradation by the induction of aggrecanase activity. TN-C fragments mapping to the EGF-L and FN type III domains 3-8 of TN-C had the highest levels of aggrecan-degrading ability that was not observed either with full-length TN-C or with other domains of TN-C. TN-C fragments represent a novel mechanism for cartilage degradation in arthritis and may present new therapeutic targets for the inhibition of cartilage degradation

Drawing on the Victorians: The Palimpsest of Victorian and Neo-Victorian Graphic Texts

Late nineteenth-century Britain experienced an unprecedented explosion of visual print culture and a simultaneous rise in literacy across social classes. New printing technologies facilitated quick and cheap dissemination of images—illustrated books, periodicals, cartoons, comics, and ephemera—to a mass readership. This Victorian visual turn prefigured the present-day impact of the Internet on how images are produced and shared, both driving and reflecting the visual culture of its time. From this starting point, Drawing on the Victorians sets out to explore the relationship between Victorian graphic texts and today’s steampunk, manga, and other neo-Victorian genres that emulate and reinterpret their predecessors. Neo-Victorianism is a flourishing worldwide phenomenon, but one whose relationship with the texts from which it takes its inspiration remains underexplored. In this collection, scholars from literary studies, cultural studies, and art history consider contemporary works—Alan Moore’s League of Extraordinary Gentlemen, Moto Naoko’s Lady Victorian, and Edward Gorey’s Gashlycrumb Tinies, among others—alongside their antecedents, from Punch’s 1897 Jubilee issue to Alice in Wonderland and more. They build on previous work on neo-Victorianism to affirm that the past not only influences but converses with the present. Contributors: Christine Ferguson, Kate Flint, Anna Maria Jones, Linda K. Hughes, Heidi Kaufman, Brian Maidment, Rebecca N. Mitchell, Jennifer Phegley, Monika Pietrzak-Franger, Peter W. Sinnema, Jessica Straleyhttps://ohioopen.library.ohio.edu/oupress/1011/thumbnail.jp

Discovery of a transient radiation belt at Saturn

Radiation belts have been detected in situ at five planets. Only at Earth however has any variability in their intensity been heretofore observed, in indirect response to solar eruptions and high altitude nuclear explosions. The Cassini spacecraft's MIMI/LEMMS instrument has now detected systematic radiation belt variability elsewhere. We report three sudden increases in energetic ion intensity around Saturn, in the vicinity of the moons Dione and Tethys, each lasting for several weeks, in response to interplanetary events caused by solar eruptions. However, the intensifications, which could create temporary satellite atmospheres at the aforementioned moons, were sharply restricted outside the orbit of Tethys. Unlike Earth, Saturn has almost unchanging inner ion radiation belts: due to Saturn's near-symmetrical magnetic field, Tethys and Dione inhibit inward radial transport of energetic ions, shielding the planet's main, inner radiation belt from solar wind influences

The prevalence of, and factors associated with, paying for sex among men resident in Britain: findings from the third national survey of sexual attitudes and lifestyles (Natsal-3)

Objectives Men who pay for sex (MPS) are considered a bridging population for sexually transmitted infections (STI). However, the extent, characteristics and role of MPS in transmission is poorly understood. We investigate these questions using data from Britain's third National Survey of Sexual Attitudes and Lifestyles (Natsal-3). Methods We performed complex survey analyses of data from 6293 men aged 16–74 years resident in Britain who completed Natsal-3, a probability sample survey undertaken during 2010–2012, using computer-assisted personal interviewing and computer-assisted self-interview. Results 11.0% (95% CI10.1% to 11.9%) of all men reported ever paying for sex. Among MPS, 18.4% (95% CI 18.2% to 18.7%) of their lifetime sexual partners were paid. 3.6% (95% CI 3.1% to 4.2%) of men had paid for sex in the past 5 years. Partners of MPS constitute 14.7% of all reported partners and MPS report 15.6% of all reported STI diagnoses in the past 5 years. Paying for sex in the past 5 years was strongly associated with reporting larger numbers of sexual partners (adjusted OR, AOR for 5+ partners, past 5 years, 31.50, 95% CI 18.69 to 53.09). After adjusting for partner numbers, paying for sex remained strongly associated with reporting new foreign partners outside the UK (AOR 7.96; 95% CI 4.97 to 12.73) and STI diagnosis/es (AOR 2.34; 95% CI 1.44 to 3.81), all in the past 5 years. Among men ever paying for sex, 62.6% (95% CI 58.3% to 66.8%) reported paying for sex outside the UK, most often in Europe and Asia. Conclusions MPS in Britain remain at greater risk of STI acquisition and onward transmission than men who do not. They report high numbers of partners, but the minority are paid partners. They are an important core group in STI transmission

Kinematics of the ring-like nebula SuWt 2

We present the first detailed spatio-kinematical analysis and modelling of the Southern planetary nebula SuWt 2. This object presents a problem for current theories of planetary nebula formation and evolution, as it is not known to contain a central post-main sequence star. Deep narrowband [NII]6584 images reveal the presence of faint bipolar lobes emanating fromthe edges of the nebular ring. Longslit observations of the H-alpha and [NII]6584 emission lines were obtained using EMMI on the 3.6-m ESO-NTT. The spectra reveal the nebular morphology as a bright torus encircling the waist of an extended bipolar structure. By deprojection, the inclination of the ring is found to be 68\degr $\pm$ 2\degr (c.f. ~90\degr for the double A-type binary believed to lie at the centre of the nebula), and the ring expansion velocity is found to be 28 km/s. Our findings are discussed with relation to possible formation scenarios for SuWt 2. Through comparison of the nebular heliocentric systemic velocity, found here to be -25 $\pm$ 5 km/s, and the heliocentric systemic velocity of the double A-type binary, we conclude that neither component of the binary could have been the nebular progenitor. However, we are unable to rule out the presence of a third component to the system, which would have been the nebula progenitor.Comment: 7 pages, 4 figures. Accepted for publication in MNRA

Abell 41: shaping of a planetary nebula by a binary central star?

We present the first detailed spatio-kinematical analysis and modelling of the planetary nebula Abell 41, which is known to contain the well-studied close-binary system MT Ser. This object represents an important test case in the study of the evolution of planetary nebulae with binary central stars as current evolutionary theories predict that the binary plane should be aligned perpendicular to the symmetry axis of the nebula. Deep narrowband imaging in the light of [NII], [OIII] and [SII], obtained using ACAM on the William Herschel Telescope, has been used to investigate the ionisation structure of Abell 41. Longslit observations of the H-alpha and [NII] emission were obtained using the Manchester Echelle Spectrometer on the 2.1-m San Pedro M\'artir Telescope. These spectra, combined with the narrowband imagery, were used to develop a spatio-kinematical model of [NII] emission from Abell 41. The best fitting model reveals Abell 41 to have a waisted, bipolar structure with an expansion velocity of ~40km\s at the waist. The symmetry axis of the model nebula is within 5\degr of perpendicular to the orbital plane of the central binary system. This provides strong evidence that the close-binary system, MT Ser, has directly affected the shaping of its nebula, Abell 41. Although the theoretical link between bipolar planetary nebulae and binary central stars is long established, this nebula is only the second to have this link, between nebular symmetry axis and binary plane, proved observationally.Comment: 7 pages, 6 figures, Accepted for publication in MNRA

Trace Gas Emissions from Biomass Burning inferred from Aerosol Optical Depth

We have observed strong correlations between simultaneous and co-located measurements of aerosol optical depth and column amounts of carbon monoxide, hydrogen cyanide, formaldehyde and ammonia in bushfire smoke plumes over SE Australia during the Austral summers of 2001/2002 and 2002/2003. We show how satellite-derived aerosol optical depth maps may be used in conjunction with these correlations to determine the total amounts of these gases present in a fire-affected region. This provides the basis of a method for estimating total emissions of trace gases from biomass burning episodes using visible radiances measured by satellite

Future eDestination Marketing: Perspective of an Australian Tourism Stakeholder Network.

Tourism destinations are difficult to manage because of the complex relationships of their diverse public and private stakeholders. At the same time, strategic marketing efforts are important for destinations to foster positive consequences of tourism, particularly given the range of opportunities and challenges created by the emergence of social media that destinations can use advantageously. This article aims to explore future eDestination marketing from Australian tourism stakeholder network perspectives. Workshops were convened in July 2012 in Melbourne, Australia, for select stakeholders invited to contribute to the futures national tourism technology strategy. They presented a stakeholder network approach to futures strategy development that aims to contribute to that used in recent national tourism plans and strategies for Australia developed by the government. Building on theories of stakeholder networks and futures, the article demonstrates the value of a futures stakeholder network method compared to traditional government approaches by critically analyzing outcomes of both