1,912 research outputs found
Entanglement-free Heisenberg-limited phase estimation
Measurement underpins all quantitative science. A key example is the
measurement of optical phase, used in length metrology and many other
applications. Advances in precision measurement have consistently led to
important scientific discoveries. At the fundamental level, measurement
precision is limited by the number N of quantum resources (such as photons)
that are used. Standard measurement schemes, using each resource independently,
lead to a phase uncertainty that scales as 1/sqrt(N) - known as the standard
quantum limit. However, it has long been conjectured that it should be possible
to achieve a precision limited only by the Heisenberg uncertainty principle,
dramatically improving the scaling to 1/N. It is commonly thought that
achieving this improvement requires the use of exotic quantum entangled states,
such as the NOON state. These states are extremely difficult to generate.
Measurement schemes with counted photons or ions have been performed with N <=
6, but few have surpassed the standard quantum limit and none have shown
Heisenberg-limited scaling. Here we demonstrate experimentally a
Heisenberg-limited phase estimation procedure. We replace entangled input
states with multiple applications of the phase shift on unentangled
single-photon states. We generalize Kitaev's phase estimation algorithm using
adaptive measurement theory to achieve a standard deviation scaling at the
Heisenberg limit. For the largest number of resources used (N = 378), we
estimate an unknown phase with a variance more than 10 dB below the standard
quantum limit; achieving this variance would require more than 4,000 resources
using standard interferometry. Our results represent a drastic reduction in the
complexity of achieving quantum-enhanced measurement precision.Comment: Published in Nature. This is the final versio
A Compromise between Neutrino Masses and Collider Signatures in the Type-II Seesaw Model
A natural extension of the standard gauge
model to accommodate massive neutrinos is to introduce one Higgs triplet and
three right-handed Majorana neutrinos, leading to a neutrino mass
matrix which contains three sub-matrices ,
and . We show that three light Majorana neutrinos (i.e., the mass
eigenstates of , and ) are exactly massless in this
model, if and only if
exactly holds. This no-go theorem implies that small but non-vanishing neutrino
masses may result from a significant but incomplete cancellation between
and terms in the Type-II
seesaw formula, provided three right-handed Majorana neutrinos are of TeV and experimentally detectable at the LHC. We propose three simple
Type-II seesaw scenarios with the flavor symmetry to
interpret the observed neutrino mass spectrum and neutrino mixing pattern. Such
a TeV-scale neutrino model can be tested in two complementary ways: (1)
searching for possible collider signatures of lepton number violation induced
by the right-handed Majorana neutrinos and doubly-charged Higgs particles; and
(2) searching for possible consequences of unitarity violation of the neutrino mixing matrix in the future long-baseline neutrino oscillation
experiments.Comment: RevTeX 19 pages, no figure
Activin promotes skin carcinogenesis by attraction and reprogramming of macrophages.
Activin has emerged as an important player in different types of cancer, but the underlying mechanisms are largely unknown. We show here that activin overexpression is an early event in murine and human skin tumorigenesis. This is functionally important, since activin promoted skin tumorigenesis in mice induced by the human papillomavirus 8 oncogenes. This was accompanied by depletion of epidermal γδ T cells and accumulation of regulatory T cells. Most importantly, activin increased the number of skin macrophages via attraction of blood monocytes, which was prevented by depletion of CCR2-positive monocytes. Gene expression profiling of macrophages from pre-tumorigenic skin and bioinformatics analysis demonstrated that activin induces a gene expression pattern in skin macrophages that resembles the phenotype of tumor-associated macrophages in different malignancies, thereby promoting angiogenesis, cell migration and proteolysis. The functional relevance of this finding was demonstrated by antibody-mediated depletion of macrophages, which strongly suppressed activin-induced skin tumor formation. These results demonstrate that activin induces skin carcinogenesis via attraction and reprogramming of macrophages and identify novel activin targets involved in tumor formation
The formation of professional identity in medical students: considerations for educators
<b>Context</b> Medical education is about more than acquiring an appropriate level of knowledge and developing relevant skills. To practice medicine students need to develop a professional identity – ways of being and relating in professional contexts.<p></p>
<b>Objectives</b> This article conceptualises the processes underlying the formation and maintenance of medical students’ professional identity drawing on concepts from social psychology.<p></p>
<b>Implications</b> A multi-dimensional model of identity and identity formation, along with the concepts of identity capital and multiple identities, are presented. The implications for educators are discussed.<p></p>
<b>Conclusions</b> Identity formation is mainly social and relational in nature. Educators, and the wider medical society, need to utilise and maximise the opportunities that exist in the various relational settings students experience. Education in its broadest sense is about the transformation of the self into new ways of thinking and relating. Helping students form, and successfully integrate their professional selves into their multiple identities, is a fundamental of medical education
The catalytic subunit of the system L1 amino acid transporter (S<i>lc7a5</i>) facilitates nutrient signalling in mouse skeletal muscle
The System L1-type amino acid transporter mediates transport of large neutral amino acids (LNAA) in many mammalian cell-types. LNAA such as leucine are required for full activation of the mTOR-S6K signalling pathway promoting protein synthesis and cell growth. The SLC7A5 (LAT1) catalytic subunit of high-affinity System L1 functions as a glycoprotein-associated heterodimer with the multifunctional protein SLC3A2 (CD98). We generated a floxed Slc7a5 mouse strain which, when crossed with mice expressing Cre driven by a global promoter, produced Slc7a5 heterozygous knockout (Slc7a5+/-) animals with no overt phenotype, although homozygous global knockout of Slc7a5 was embryonically lethal. Muscle-specific (MCK Cre-mediated) Slc7a5 knockout (MS-Slc7a5-KO) mice were used to study the role of intracellular LNAA delivery by the SLC7A5 transporter for mTOR-S6K pathway activation in skeletal muscle. Activation of muscle mTOR-S6K (Thr389 phosphorylation) in vivo by intraperitoneal leucine injection was blunted in homozygous MS-Slc7a5-KO mice relative to wild-type animals. Dietary intake and growth rate were similar for MS-Slc7a5-KO mice and wild-type littermates fed for 10 weeks (to age 120 days) with diets containing 10%, 20% or 30% of protein. In MS-Slc7a5-KO mice, Leu and Ile concentrations in gastrocnemius muscle were reduced by ∼40% as dietary protein content was reduced from 30 to 10%. These changes were associated with >50% decrease in S6K Thr389 phosphorylation in muscles from MS-Slc7a5-KO mice, indicating reduced mTOR-S6K pathway activation, despite no significant differences in lean tissue mass between groups on the same diet. MS-Slc7a5-KO mice on 30% protein diet exhibited mild insulin resistance (e.g. reduced glucose clearance, larger gonadal adipose depots) relative to control animals. Thus, SLC7A5 modulates LNAA-dependent muscle mTOR-S6K signalling in mice, although it appears non-essential (or is sufficiently compensated by e.g. SLC7A8 (LAT2)) for maintenance of normal muscle mass
Bacteriophage Lysin Mediates the Binding of Streptococcus mitis to Human Platelets through Interaction with Fibrinogen
The binding of bacteria to human platelets is a likely central mechanism in the pathogenesis of infective endocarditis. We have previously found that platelet binding by Streptococcus mitis SF100 is mediated by surface components encoded by a lysogenic bacteriophage, SM1. We now demonstrate that SM1-encoded lysin contributes to platelet binding via its direct interaction with fibrinogen. Far Western blotting of platelets revealed that fibrinogen was the major membrane-associated protein bound by lysin. Analysis of lysin binding with purified fibrinogen in vitro confirmed that these proteins could bind directly, and that this interaction was both saturable and inhibitable. Lysin bound both the Aα and Bβ chains of fibrinogen, but not the γ subunit. Binding of lysin to the Bβ chain was further localized to a region within the fibrinogen D fragment. Disruption of the SF100 lysin gene resulted in an 83±3.1% reduction (mean ± SD) in binding to immobilized fibrinogen by this mutant strain (PS1006). Preincubation of this isogenic mutant with purified lysin restored fibrinogen binding to wild type levels. When tested in a co-infection model of endocarditis, loss of lysin expression resulted in a significant reduction in virulence, as measured by achievable bacterial densities (CFU/g) within vegetations, kidneys, and spleens. These results indicate that bacteriophage-encoded lysin is a multifunctional protein, representing a new class of fibrinogen-binding proteins. Lysin appears to be cell wall-associated through its interaction with choline. Once on the bacterial surface, lysin can bind fibrinogen directly, which appears to be an important interaction for the pathogenesis of endocarditis
A comparison of laboratory and in situ methods to determine soil thermal conductivity for energy foundations and other ground heat exchanger applications
Soil thermal conductivity is an important factor in the design of energy foundations and other ground heat exchanger systems. It can be determined by a field thermal response test, which is both costly and time consuming, but tests a large volume of soil. Alternatively, cheaper and quicker laboratory test methods may be applied to smaller soil samples. This paper investigates two different laboratory methods: the steady-state thermal cell and the transient needle probe. U100 soil samples were taken during the site investigation for a small diameter test pile, for which a thermal response test was later conducted. The thermal conductivities of the samples were measured using the two laboratory methods. The results from the thermal cell and needle probe were significantly different, with the thermal cell consistently giving higher values for thermal conductivity. The main difficulty with the thermal cell was determining the rate of heat flow, as the apparatus experiences significant heat losses. The needle probe was found to have fewer significant sources of error, but tests a smaller soil sample than the thermal cell. However, both laboratory methods gave much lower values of thermal conductivity compared to the in situ thermal response test. Possible reasons for these discrepancies are discussed, including sample size, orientation and disturbance
Macrophage-derived human resistin is induced in multiple helminth infections and promotes inflammatory monocytes and increased parasite burden.
Parasitic helminth infections can be associated with lifelong morbidity such as immune-mediated organ failure. A better understanding of the host immune response to helminths could provide new avenues to promote parasite clearance and/or alleviate infection-associated morbidity. Murine resistin-like molecules (RELM) exhibit pleiotropic functions following helminth infection including modulating the host immune response; however, the relevance of human RELM proteins in helminth infection is unknown. To examine the function of human resistin (hResistin), we utilized transgenic mice expressing the human resistin gene (hRetnTg+). Following infection with the helminth Nippostrongylus brasiliensis (Nb), hResistin expression was significantly upregulated in infected tissue. Compared to control hRetnTg- mice, hRetnTg+ mice suffered from exacerbated Nb-induced inflammation characterized by weight loss and increased infiltration of inflammatory monocytes in the lung, along with elevated Nb egg burdens and delayed parasite expulsion. Genome-wide transcriptional profiling of the infected tissue revealed that hResistin promoted expression of proinflammatory cytokines and genes downstream of toll-like receptor signaling. Moreover, hResistin preferentially bound lung monocytes, and exogenous treatment of mice with recombinant hResistin promoted monocyte recruitment and proinflammatory cytokine expression. In human studies, increased serum resistin was associated with higher parasite load in individuals infected with soil-transmitted helminths or filarial nematode Wuchereria bancrofti, and was positively correlated with proinflammatory cytokines. Together, these studies identify human resistin as a detrimental factor induced by multiple helminth infections, where it promotes proinflammatory cytokines and impedes parasite clearance. Targeting the resistin/proinflammatory cytokine immune axis may provide new diagnostic or treatment strategies for helminth infection and associated immune-mediated pathology
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