Examining the varying influences of built environment on bike-sharing commuting: Empirical evidence from Shanghai

Commute behaviors, as the primary part of urban mobility, remains largely underexplored, especially for bike-sharing users. Recent development in data availability open up new possibilities to delve into bike-sharing commuting over long-term periods on a large scale. This study proposes a methodological framework that enables a logical identification of bike-sharing commuting activities and a comprehensive examination of urban built environment effects on shaping commuting patterns. To this end, a series of data mining methods are developed in support of the identification of regular bike-sharing commuting, and the concepts of home-work balance and mobility trend are proposed to describe underlying commuting patterns. The XGBoost model and Necessary Condition Analysis (NCA) method are then adopted respectively to test the sufficiency and necessity of built environment on commuting patterns. The results confirm the massive existence of individual-level bike-sharing commuting activities and the pivotal role of bike-sharing in urban commuting. Also, the spatial distributions of home-work balance and mobility trend driven by job-housing separation show different clustering patterns. Besides, the synergy of sufficiency analysis and necessity analysis investigates the complex interplay of built environment-commuting patterns. This critical analysis of bike-sharing commute provides insights into sustainable transit planning and urban design

Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from <i>Penicillium</i> sp. OUCMDZ-1435

Meleagrin and oxaline, which belong to the roquefortine alkaloids with a unique dihydroindole spiroamide framework, have significant bioactivities, especially tumor cell inhibitory activity. In order to discover the requefortine alkaloids, Penicillium sp. OUCMDZ-1435 was fished and identified from marine fungi using molecular probe technology. Meleagrin (1) and oxaline (2) were isolated from it. In addition, we first reported that compounds 1 and 2 could effectively inhibit the proliferation and metastasis of the human HepG2 cell and induce HepG2 cell apoptosis and cell cycle arrest in the G2/M phase. Additionally, the fermentation of Meleagrin (1) was optimized to increase its yield to 335 mg/L. These results provided bioactive inspiration and fungus resources for roquefortine alkaloid development